FastQCFastQC Report
Thu 26 May 2016
SRR936667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253001
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52820.4215479476871926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42970.3429366776243594No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39840.31795664967545917No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26960.2151634356237545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.18539490391468164No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17570.14022335177705364No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16290.1300078770886855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG61600.026.3762871
TACATGG62050.025.802182
ACATGGG62000.023.9063823
TACCTGG12000.023.7540842
GCGTTCG601.7188863E-423.73512634-35
GTACCTG12600.022.9999851
GAGTACT49000.022.40073412-13
CATGGGG26500.022.050964
GTATCAA107650.021.7571091
GTGTAGC6650.020.7178481
GTACTTT53550.020.45385214-15
AGTACTT51800.020.04324312-13
TAGACTG4850.019.591785
TACCGCT1507.7105896E-419.0040257
TATCAAC124050.018.7658212
ATCAACG123450.018.740833
AGAGTAC90650.018.68730710-11
TCAACGC123900.018.6351664
CAACGCA124050.018.5750775
ATGGGCG1559.61149E-418.3909935
Produced by FastQC (version 0.11.5)