##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936667_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253001 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58738420799345 31.0 31.0 34.0 30.0 34.0 2 31.658054542653996 33.0 31.0 34.0 30.0 34.0 3 31.680119967980872 33.0 31.0 34.0 30.0 34.0 4 35.29537007552268 37.0 35.0 37.0 33.0 37.0 5 35.31183055719828 37.0 35.0 37.0 33.0 37.0 6 35.297788269921575 37.0 35.0 37.0 33.0 37.0 7 35.23239726065662 37.0 35.0 37.0 32.0 37.0 8 35.19077478788923 37.0 35.0 37.0 32.0 37.0 9 36.72773764745599 39.0 37.0 39.0 32.0 39.0 10-11 36.602437268605534 39.0 37.0 39.0 32.0 39.0 12-13 36.515724648264445 39.0 37.0 39.0 32.0 39.0 14-15 37.65737816649788 40.0 37.0 41.0 32.0 41.0 16-17 37.55744488631693 40.0 37.0 41.0 32.0 41.0 18-19 37.54053907379164 40.0 37.0 41.0 32.0 41.0 20-21 37.42082847499722 40.0 37.0 41.0 32.0 41.0 22-23 37.540132050972026 40.0 37.0 41.0 32.0 41.0 24-25 37.58406218350983 40.0 37.0 41.0 32.0 41.0 26-27 37.487661621977956 40.0 37.0 41.0 32.0 41.0 28-29 37.35924951376735 40.0 37.0 41.0 31.5 41.0 30-31 37.129334693268405 39.5 36.5 41.0 31.0 41.0 32-33 36.94532685927625 39.0 36.0 41.0 31.0 41.0 34-35 36.75393515248591 39.0 36.0 41.0 30.0 41.0 36-37 36.559827166937616 39.0 35.0 41.0 30.0 41.0 38-39 36.35518247790704 39.0 35.0 40.5 30.0 41.0 40-41 36.148865802980204 39.0 35.0 40.0 30.0 41.0 42-43 35.91072034260148 39.0 35.0 40.0 29.0 41.0 44-45 35.609785227625515 38.0 35.0 40.0 27.5 41.0 46-47 35.388295380450614 38.5 35.0 40.0 26.5 41.0 48-49 35.195960737461505 39.0 35.0 40.0 26.0 41.0 50-51 34.231504603747325 38.0 34.0 39.5 24.0 40.5 52-53 34.33594665926044 38.0 33.5 39.5 24.0 40.5 54-55 34.77147344654952 38.0 34.0 40.0 25.0 41.0 56-57 34.65797553234195 38.0 34.0 40.0 24.5 41.0 58-59 34.42193062894603 38.0 34.0 40.0 24.0 41.0 60-61 34.13141569719417 38.0 34.0 40.0 23.0 41.0 62-63 33.77671965146077 37.0 33.0 40.0 22.0 41.0 64-65 33.426981303287064 37.0 33.0 40.0 22.0 41.0 66-67 32.976817656171065 36.0 32.5 39.0 20.0 41.0 68-69 32.583224993435756 36.0 32.0 39.0 19.0 40.5 70-71 32.17459443368361 35.0 31.5 38.5 18.0 40.0 72-73 31.793979015180355 35.0 31.0 38.0 16.5 40.0 74-75 31.369413112998313 35.0 31.0 37.0 12.5 39.0 76-77 30.91198650280407 35.0 31.0 36.5 8.0 39.0 78-79 30.418617383385964 34.5 30.0 36.0 7.0 39.0 80-81 29.94809102307181 34.0 30.0 36.0 3.5 37.5 82-83 29.53802550835953 34.0 29.0 35.0 2.0 37.0 84-85 29.17438852802192 34.0 29.0 35.0 2.0 36.5 86-87 28.828160153104427 34.0 29.0 35.0 2.0 36.0 88-89 28.411728322643 34.0 29.0 35.0 2.0 36.0 90-91 27.994015168383743 34.0 27.5 35.0 2.0 35.5 92-93 27.543920555530285 34.0 26.5 35.0 2.0 35.0 94-95 27.0599692258825 33.0 25.5 35.0 2.0 35.0 96-97 26.492841186878543 33.0 25.0 35.0 2.0 35.0 98-99 25.756858534031497 33.0 23.5 35.0 2.0 35.0 100-101 22.824889605036226 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36735184233950235 1101 2 0.48836140648758075 1101 3 0.4860584409951052 1101 4 0.4434463656671781 1101 5 0.4919117952794352 1101 6 0.46753414964535267 1101 7 0.4339656320260801 1101 8 0.3996497947204958 1101 9 0.505152457425396 1101 10-11 0.652675830015113 1101 12-13 0.725180122859598 1101 14-15 0.7618171877965239 1101 16-17 0.7974422700553845 1101 18-19 0.6843253180358886 1101 20-21 0.638921455250518 1101 22-23 0.65386084256847 1101 24-25 0.6047271215316812 1101 26-27 0.6648073408366386 1101 28-29 0.7398734892653565 1101 30-31 0.5911355615033074 1101 32-33 0.5038472004619976 1101 34-35 0.47537803656592104 1101 36-37 0.3746274234120932 1101 38-39 0.32430361844682665 1101 40-41 0.37069200668953073 1101 42-43 0.4164944454857107 1101 44-45 0.49512722421510347 1101 46-47 0.7990069914301543 1101 48-49 0.7319454064791984 1101 50-51 0.6556638820917584 1101 52-53 0.8937557380818646 1101 54-55 0.5738206753829544 1101 56-57 0.6633336508263241 1101 58-59 0.6144869840356435 1101 60-61 0.7679399853074784 1101 62-63 0.7050268941886202 1101 64-65 0.7605028304612418 1101 66-67 0.8327908762380716 1101 68-69 0.7657176662210006 1101 70-71 0.5574621294660815 1101 72-73 0.3272471870554092 1101 74-75 0.2923423086971866 1101 76-77 0.387390468640735 1101 78-79 0.4566555419745626 1101 80-81 0.4366033651895407 1101 82-83 0.4315859409438296 1101 84-85 0.20078186068713322 1101 86-87 0.3774182885767914 1101 88-89 0.4315446172195827 1101 90-91 0.18820037520996635 1101 92-93 0.17099534250096937 1101 94-95 0.06223904893458965 1101 96-97 0.08928089438915521 1101 98-99 0.35518891050463 1101 100-101 0.6534354235443054 1102 1 0.22212831455062343 1102 2 0.2715082261337045 1102 3 0.24932013018161214 1102 4 0.21340815328972695 1102 5 0.24956270493193955 1102 6 0.21620548796320804 1102 7 0.3739871185227983 1102 8 0.2542039799017104 1102 9 0.5364536949606418 1102 10-11 0.45353064172338264 1102 12-13 0.36835508636778513 1102 14-15 0.2986394278950044 1102 16-17 0.2801923467125462 1102 18-19 0.25698262640610636 1102 20-21 0.20024980955430038 1102 22-23 0.1756620272496363 1102 24-25 0.23624063755771374 1102 26-27 0.020598161619311384 1102 28-29 -0.1497270763108176 1102 30-31 0.1823230990353295 1102 32-33 0.09001453723846708 1102 34-35 0.15918485751652867 1102 36-37 0.022320661313187884 1102 38-39 -0.16010873786316182 1102 40-41 -0.21399785229877466 1102 42-43 -0.17071760284616744 1102 44-45 -0.0875468700120976 1102 46-47 0.3204728713081977 1102 48-49 0.5260624471603492 1102 50-51 0.537679511906255 1102 52-53 0.6460275265215927 1102 54-55 0.6218269893841892 1102 56-57 0.5679991294631535 1102 58-59 0.42120522529166493 1102 60-61 0.48218007479206904 1102 62-63 0.5034729091781855 1102 64-65 0.6493908030460958 1102 66-67 0.5445498226350267 1102 68-69 0.4393611343185029 1102 70-71 0.552971594095446 1102 72-73 0.29427804709254346 1102 74-75 0.23182175970444874 1102 76-77 0.3998915838243988 1102 78-79 0.34771053196227086 1102 80-81 0.5290072963337913 1102 82-83 0.29880617603579296 1102 84-85 -0.1437054959414823 1102 86-87 0.137965676947843 1102 88-89 0.09619933011695636 1102 90-91 0.14309335131466838 1102 92-93 0.17978149038294688 1102 94-95 -0.1041789661359509 1102 96-97 -0.0018768424364985492 1102 98-99 0.1702590340032586 1102 100-101 0.43445645946933453 1103 1 0.42404422988611046 1103 2 0.31223590239174825 1103 3 0.35443970722561957 1103 4 0.49353901904652275 1103 5 0.6377236361549379 1103 6 0.44919993774885114 1103 7 0.5485235314947374 1103 8 0.5517986093070135 1103 9 0.290899198387244 1103 10-11 0.60437762842853 1103 12-13 0.26622451886628085 1103 14-15 0.5648483115704437 1103 16-17 0.608607244814209 1103 18-19 0.8018067307654562 1103 20-21 0.8152624229204619 1103 22-23 0.5318627797194111 1103 24-25 0.5252210626798046 1103 26-27 0.5498759172732477 1103 28-29 0.49028420407082507 1103 30-31 0.5058390706894116 1103 32-33 0.39555916972629745 1103 34-35 0.7358310517623678 1103 36-37 0.6779318180605074 1103 38-39 0.7232184581871977 1103 40-41 0.7635196972757541 1103 42-43 0.7093308678040131 1103 44-45 0.8652131994363899 1103 46-47 0.9740960065877999 1103 48-49 1.1796241401840035 1103 50-51 1.0200916140728609 1103 52-53 1.0448579575588042 1103 54-55 1.0769830985356208 1103 56-57 1.1268495635995563 1103 58-59 0.9635541439912672 1103 60-61 1.1394416330176682 1103 62-63 1.1695675727412222 1103 64-65 1.0579141144462696 1103 66-67 0.9843070444953099 1103 68-69 1.104136559748845 1103 70-71 1.1906364051365657 1103 72-73 0.9803787257482988 1103 74-75 0.9995333751650683 1103 76-77 0.9075041751963226 1103 78-79 0.8808354059535795 1103 80-81 0.9679547300886249 1103 82-83 0.8663991142169643 1103 84-85 0.9633317043244496 1103 86-87 1.0783148272535144 1103 88-89 0.9559849778724576 1103 90-91 0.7337998247874467 1103 92-93 0.9218943875081997 1103 94-95 0.9070268516750595 1103 96-97 1.0999817129081855 1103 98-99 1.0278217217666246 1103 100-101 0.6858673402888549 1104 1 0.4279664001948511 1104 2 0.3704476169364135 1104 3 0.3044062664130074 1104 4 0.45884322593875027 1104 5 0.4095689419958717 1104 6 0.5387704893132508 1104 7 0.636429642613237 1104 8 0.5413569055736147 1104 9 0.6292912463140112 1104 10-11 0.7063737694347552 1104 12-13 0.6693509023373991 1104 14-15 0.3606543871580783 1104 16-17 0.41778655787151564 1104 18-19 0.4007326346649265 1104 20-21 0.22814459345720906 1104 22-23 0.3938887894514451 1104 24-25 0.4734697426227328 1104 26-27 0.2902930344091956 1104 28-29 0.43214231622294363 1104 30-31 0.43484930813818323 1104 32-33 0.3281270061247241 1104 34-35 0.4659413684430689 1104 36-37 0.3540701357216278 1104 38-39 0.33012360598228696 1104 40-41 0.22551782383023067 1104 42-43 0.39168827408899176 1104 44-45 0.1688123143948843 1104 46-47 -0.09821405054658783 1104 48-49 0.20539565393179515 1104 50-51 -0.015082040646952066 1104 52-53 0.1442671857030433 1104 54-55 0.32817514000223014 1104 56-57 0.1475230159644525 1104 58-59 0.08835677669524244 1104 60-61 0.18953183770963733 1104 62-63 0.21420131984127977 1104 64-65 0.30230304059718094 1104 66-67 0.17596731433987145 1104 68-69 0.2750526005086371 1104 70-71 0.3153174348855856 1104 72-73 0.02000822624516374 1104 74-75 0.2788078615350891 1104 76-77 0.09162007421267049 1104 78-79 0.21803113730985046 1104 80-81 0.3484757892503616 1104 82-83 0.10465421631594651 1104 84-85 0.09542600171587878 1104 86-87 0.22538433854263928 1104 88-89 0.08023160956560815 1104 90-91 -7.026861235708282E-4 1104 92-93 -0.012847101709990483 1104 94-95 0.12666221381435605 1104 96-97 0.10366233984742124 1104 98-99 0.27859124046592854 1104 100-101 0.018211066241395457 1105 1 0.24866409526151756 1105 2 0.20597110888965986 1105 3 0.26692726130698574 1105 4 0.17552865688767838 1105 5 0.27968560977456036 1105 6 0.42200026447284245 1105 7 0.4776990502485745 1105 8 0.4780919414744176 1105 9 0.5346131400022216 1105 10-11 0.5384309039160797 1105 12-13 0.2515544822787632 1105 14-15 0.4385515511859239 1105 16-17 0.43556674415540186 1105 18-19 0.3480419521686642 1105 20-21 0.3802989309484559 1105 22-23 0.1472896076547059 1105 24-25 0.2430914641488684 1105 26-27 -0.009041223216080141 1105 28-29 -0.06720202840801193 1105 30-31 0.11422463878970035 1105 32-33 0.027330395358625026 1105 34-35 0.16582536787777258 1105 36-37 0.3507892320520085 1105 38-39 0.10473629385933947 1105 40-41 0.0670023683835339 1105 42-43 0.07083139346397616 1105 44-45 -0.02063636244739797 1105 46-47 0.1293942416025189 1105 48-49 0.21445557127088222 1105 50-51 0.24364912961907947 1105 52-53 0.3502199375380144 1105 54-55 0.23473847089962874 1105 56-57 -0.0093341252415442 1105 58-59 0.19986363349496372 1105 60-61 0.14548498356826656 1105 62-63 0.11555303113856041 1105 64-65 0.014133049333999281 1105 66-67 0.10371756007929633 1105 68-69 0.2588075304013202 1105 70-71 0.20171699904287976 1105 72-73 0.09180248915561151 1105 74-75 0.1910932040125779 1105 76-77 0.08802762916977969 1105 78-79 0.10822830149530915 1105 80-81 0.11472440557061248 1105 82-83 0.10096195194603652 1105 84-85 0.04837012022978726 1105 86-87 0.02615599447697292 1105 88-89 -0.025866090439485845 1105 90-91 -0.16530948466032314 1105 92-93 -0.34166247220518287 1105 94-95 -0.06663003248300114 1105 96-97 0.017475706075604336 1105 98-99 0.18250436823099037 1105 100-101 0.32438321044985585 1106 1 0.3351146008405692 1106 2 0.27269385679781877 1106 3 0.22782543327653926 1106 4 0.27676195969731054 1106 5 0.32054618429841497 1106 6 0.33225816550822884 1106 7 0.45866958276600656 1106 8 0.4634472276462134 1106 9 0.3614256663470812 1106 10-11 0.49647535223655126 1106 12-13 0.45678003061522077 1106 14-15 0.6631142265155106 1106 16-17 0.6399005038515142 1106 18-19 0.5458621724238029 1106 20-21 0.326887604717939 1106 22-23 0.46937701687092925 1106 24-25 0.36780197350704924 1106 26-27 0.4568218949493925 1106 28-29 0.3707939817084167 1106 30-31 0.5896400396918438 1106 32-33 0.419974789414745 1106 34-35 0.5334663704117233 1106 36-37 0.38111458804070963 1106 38-39 0.3713965408590596 1106 40-41 0.4990777498086487 1106 42-43 0.48423493130609074 1106 44-45 0.23177494924802744 1106 46-47 0.23707809844152195 1106 48-49 0.38478477905975694 1106 50-51 0.06563297628351705 1106 52-53 0.15608406104756511 1106 54-55 0.26727467539872407 1106 56-57 0.27457534291932717 1106 58-59 0.2442253490237718 1106 60-61 0.2279846686412128 1106 62-63 0.43131979373686136 1106 64-65 0.3468341060354092 1106 66-67 0.2031397294906654 1106 68-69 0.6742523406456584 1106 70-71 0.5214015753690902 1106 72-73 0.153468538080741 1106 74-75 0.35205181775211614 1106 76-77 0.24028335478594443 1106 78-79 0.09978855915409213 1106 80-81 0.38712056442951237 1106 82-83 0.3221270488224448 1106 84-85 0.34937316290967146 1106 86-87 0.3420175380071626 1106 88-89 0.19591493108698899 1106 90-91 0.08615946837678479 1106 92-93 0.23699540557404575 1106 94-95 0.297354788032802 1106 96-97 0.5777597097190643 1106 98-99 0.9180116672711875 1106 100-101 0.9641959863138432 1107 1 0.21137151386218989 1107 2 0.11497762721251803 1107 3 0.22781732416027367 1107 4 0.278306746346459 1107 5 0.2980271684207594 1107 6 0.32140005882469325 1107 7 0.3034996156890202 1107 8 0.46668073775828134 1107 9 0.4155236082533733 1107 10-11 0.5900140083938012 1107 12-13 0.4454830180136611 1107 14-15 0.27515079052076175 1107 16-17 0.433529576655161 1107 18-19 0.32654301382570594 1107 20-21 0.42976086733977326 1107 22-23 0.2004766941280991 1107 24-25 0.2217871986972071 1107 26-27 0.2662130624999506 1107 28-29 0.2838814871820645 1107 30-31 0.3566377610301714 1107 32-33 0.25625375923261373 1107 34-35 0.29137681333165943 1107 36-37 0.2852577382701966 1107 38-39 0.18283323298835086 1107 40-41 0.4617281739154322 1107 42-43 0.507528367787387 1107 44-45 0.632453479551792 1107 46-47 0.5402475465350633 1107 48-49 0.3449112407279671 1107 50-51 0.24169810870538555 1107 52-53 0.4842670919108585 1107 54-55 0.4629131872137009 1107 56-57 0.36026538816819453 1107 58-59 0.39440010047936624 1107 60-61 0.3842584891702501 1107 62-63 0.4205640646462143 1107 64-65 0.3630604476886887 1107 66-67 0.36874308828662805 1107 68-69 0.40580916933246414 1107 70-71 0.17682799324806098 1107 72-73 0.19156820722879786 1107 74-75 0.24872343097970528 1107 76-77 0.40138108585521337 1107 78-79 0.3337619044889166 1107 80-81 0.4342466936239511 1107 82-83 0.42237701233142033 1107 84-85 0.2693949764324337 1107 86-87 0.20371453276867157 1107 88-89 0.021673336186001535 1107 90-91 0.1647763369604469 1107 92-93 0.1953023842795112 1107 94-95 0.2206648482024427 1107 96-97 0.24897457697283087 1107 98-99 0.1581307090980104 1107 100-101 0.26716777469834696 1108 1 0.26040394964485003 1108 2 0.330906652509956 1108 3 0.3555455753470973 1108 4 0.4057884136092227 1108 5 0.17514914156606665 1108 6 0.3459473773911341 1108 7 0.3038350586848111 1108 8 0.30816800166158487 1108 9 0.4025042732598578 1108 10-11 0.5620045182449402 1108 12-13 0.1884174259493605 1108 14-15 0.3539758218250739 1108 16-17 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-2.104555110567908 2316 74-75 -2.0046182714547385 2316 76-77 -1.8796104360637287 2316 78-79 -2.8334912279859914 2316 80-81 -2.7422983765703144 2316 82-83 -2.800446811802889 2316 84-85 -2.4358320790970396 2316 86-87 -2.2714865911219704 2316 88-89 -2.5240357180916 2316 90-91 -2.7062441484753634 2316 92-93 -3.1879074384464445 2316 94-95 -3.2654957463250867 2316 96-97 -3.7848556630065993 2316 98-99 -4.912947968835503 2316 100-101 -5.642970923242775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2721.0 3 1077.0 4 1053.0 5 1075.0 6 1267.0 7 1387.0 8 1788.0 9 2440.0 10 2976.0 11 3792.0 12 4820.0 13 5944.0 14 7026.0 15 8555.0 16 10714.0 17 13695.0 18 10828.0 19 7887.0 20 7776.0 21 8396.0 22 9078.0 23 10314.0 24 11527.0 25 13484.0 26 15597.0 27 18363.0 28 21806.0 29 26456.0 30 32439.0 31 39879.0 32 50204.0 33 65225.0 34 88173.0 35 121760.0 36 173480.0 37 233167.0 38 188393.0 39 28432.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.961016321422186 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26.856630924494496 29.10671173346209 22.31524675666724 44-45 21.912419437287085 26.78514920587542 29.099265872216197 22.203165484621305 46-47 22.199401272634837 27.163323599291616 28.651722159217424 21.98555296885612 48-49 22.243256411780973 27.24087609882469 28.394240480643646 22.121627008750693 50-51 21.885168510450146 27.51301130373223 28.52602141028885 22.075798775528774 52-53 22.324296433512604 27.595034300898952 28.024778214389894 22.055891051198547 54-55 21.869500430587603 27.313340872939545 28.281145371003696 22.536013325469156 56-57 22.142191593325578 27.55654962020922 28.02091792249355 22.280340863971652 58-59 22.557845618115362 27.191999843566116 27.968462610107302 22.281691928211213 60-61 22.066931985377007 27.276043061282557 28.779125565611313 21.877899387729123 62-63 21.915539134449514 27.462331239283174 28.641187172010728 21.98094245425658 64-65 21.72485676273812 27.797765506695576 28.299684514892764 22.177693215673536 66-67 21.752471082566846 27.730603109377384 27.907089051565663 22.60983675649011 68-69 21.711719768006336 27.54214147014487 28.320656825672604 22.42548193617619 70-71 22.00447333685217 27.478881010996485 28.054657436782776 22.46198821536857 72-73 21.958609745954487 27.335415590749413 28.367178663593034 22.338795999703066 74-75 21.692287856270656 28.096487519923937 27.961006772839823 22.25021785096559 76-77 21.8386386415154 27.651267043225197 27.922290197543546 22.587804117715855 78-79 21.95589974470627 27.65752629397668 27.920845368019847 22.4657285932972 80-81 22.01223449949938 27.812658876961287 27.790932795719325 22.384173827820007 82-83 21.90419079915792 27.778152621280615 28.06373013822942 22.253926441332045 84-85 21.78675853723368 27.85878891454744 27.833205750105567 22.52124679811331 86-87 21.840037900906054 27.966366538002084 27.742537261308307 22.451058299783554 88-89 21.777845895702143 27.860244370392813 27.462832159696088 22.899077574208956 90-91 21.769333181634 27.585605330235474 27.816188314760144 22.82887317337038 92-93 21.991036774132773 27.600676632547653 27.929014252710598 22.47927234060897 94-95 21.701228418859703 27.341977762346048 28.04925648332176 22.90753733547249 96-97 21.414972947860974 27.32673140873874 28.328035722553135 22.93025992084715 98-99 21.405728859330498 27.547089058186895 27.81913521740137 23.228046865081232 100-101 21.281812406184525 27.42444746952282 28.02522590586966 23.268514218422993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 157.0 1 126.0 2 165.0 3 237.0 4 218.5 5 235.5 6 381.5 7 714.5 8 1201.0 9 1519.5 10 1476.5 11 1382.0 12 1423.0 13 1544.5 14 1674.0 15 1765.0 16 1858.0 17 1964.5 18 2100.0 19 2324.5 20 2585.5 21 2979.0 22 3541.0 23 4189.5 24 5051.5 25 6121.5 26 7689.5 27 9582.5 28 11346.5 29 13268.0 30 15788.5 31 18734.5 32 21978.0 33 24412.5 34 26441.0 35 28333.5 36 30653.0 37 33012.5 38 34269.0 39 35104.0 40 36162.0 41 38109.5 42 39797.5 43 41554.0 44 43132.0 45 44824.0 46 45710.0 47 48445.5 48 56481.5 49 55649.5 50 47510.5 51 43218.0 52 40151.0 53 39256.0 54 38346.5 55 35361.0 56 32093.0 57 28957.0 58 26279.0 59 23262.0 60 19566.0 61 16292.0 62 13431.0 63 10504.0 64 7867.0 65 6013.5 66 4439.0 67 3449.0 68 2570.0 69 1684.5 70 1226.0 71 953.5 72 765.5 73 658.0 74 511.0 75 418.0 76 328.0 77 196.5 78 118.0 79 77.0 80 45.5 81 34.5 82 25.0 83 11.0 84 7.5 85 8.0 86 5.5 87 5.5 88 7.5 89 4.5 90 2.0 91 1.5 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013487618924486093 2 0.012370301380445824 3 0.016201104388583888 4 0.01979248220871332 5 0.03024738208509012 6 0.049880247501797675 7 0.007422180828267496 8 0.026177153888943427 9 0.03727052093334323 10-11 0.04022343158544965 12-13 0.029768531709072857 14-15 0.025738207710927608 16-17 0.030526711471100185 18-19 0.024660794364888775 20-21 0.05602549399401916 22-23 0.013846756706499036 24-25 0.002673581266096356 26-27 0.0014365511280517733 28-29 0.0 30-31 0.0023543476820848505 32-33 0.0017956889100647167 34-35 0.0 36-37 4.389461780158196E-4 38-39 7.98083960028763E-5 40-41 9.577007520345156E-4 42-43 0.0036711862161323094 44-45 0.0013966469300503352 46-47 7.98083960028763E-4 48-49 0.0011572217420417062 50-51 0.004150036592149567 52-53 0.0036312820181308714 54-55 0.00494812055217833 56-57 6.783713660244485E-4 58-59 0.00602553389821716 60-61 0.003391856830122242 62-63 5.187545740186959E-4 64-65 7.98083960028763E-4 66-67 0.01675976316060402 68-69 0.04413404298959059 70-71 0.0552274100339904 72-73 0.015482828824558002 74-75 0.03387866410322099 76-77 0.02717475883897938 78-79 0.05730242833006518 80-81 0.08375891160501868 82-83 0.049999960095802 84-85 0.01787708070464429 86-87 0.020790087158749276 88-89 0.021667979514780916 90-91 0.04177969530750574 92-93 0.026097345492940547 94-95 0.013647235716491847 96-97 0.01875497306067593 98-99 0.05107737344184083 100-101 0.0698722507005182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.49845924418387 #Duplication Level Percentage of deduplicated Percentage of total 1 82.71769604282963 52.52446250948406 2 10.631953261885045 13.502253017717504 3 2.851455744318266 5.431891391015621 4 1.2158688484324023 3.0882319407383068 5 0.6521797044402687 2.0706203191142145 6 0.3848125901586258 1.46610039437018 7 0.2793311338726583 1.2415967633891263 8 0.19467917164017612 0.988946195686816 9 0.13924020699099565 0.7957384747892516 >10 0.8368848273852036 9.817149777469428 >50 0.06300533839441122 2.7468582975362086 >100 0.029994513771117375 3.5898539766723068 >500 0.0016383481068071334 0.7017397679216978 >1k 0.0011342409970203232 1.6118647970668076 >5k 1.2602677744670256E-4 0.4226923770284419 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5282 0.4215479476871926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4297 0.3429366776243594 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3984 0.31795664967545917 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2696 0.2151634356237545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2323 0.18539490391468164 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1757 0.14022335177705364 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1629 0.1300078770886855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.980839600287629E-5 0.0 0.0 0.0 0.0 7 7.980839600287629E-5 0.0 0.0 0.0 0.0 8 7.980839600287629E-5 0.0 0.0 0.0 7.980839600287629E-5 9 7.980839600287629E-5 0.0 0.0 0.0 7.980839600287629E-5 10-11 1.5961679200575258E-4 0.0 0.0 0.0 7.980839600287629E-5 12-13 1.5961679200575258E-4 0.0 0.0 0.0 7.980839600287629E-5 14-15 1.5961679200575258E-4 0.0 0.0 0.0 7.980839600287629E-5 16-17 1.9952099000719072E-4 0.0 0.0 3.9904198001438144E-5 7.980839600287629E-5 18-19 2.3942518800862888E-4 0.0 0.0 1.5961679200575258E-4 7.980839600287629E-5 20-21 2.3942518800862888E-4 0.0 0.0 1.5961679200575258E-4 7.980839600287629E-5 22-23 2.3942518800862888E-4 0.0 0.0 3.9904198001438143E-4 7.980839600287629E-5 24-25 3.192335840115052E-4 0.0 0.0 5.985629700215721E-4 7.980839600287629E-5 26-27 3.990419800143815E-4 0.0 0.0 8.379881580302011E-4 7.980839600287629E-5 28-29 3.990419800143815E-4 0.0 0.0 0.0018355931080661547 7.980839600287629E-5 30-31 3.990419800143815E-4 0.0 0.0 0.0037908988101366245 7.980839600287629E-5 32-33 3.990419800143815E-4 0.0 0.0 0.007980839600287629 7.980839600287629E-5 34-35 3.990419800143815E-4 0.0 0.0 0.011492409024414186 7.980839600287629E-5 36-37 3.990419800143815E-4 0.0 0.0 0.014565032270524924 7.980839600287629E-5 38-39 3.990419800143815E-4 0.0 0.0 0.019114110842688874 7.980839600287629E-5 40-41 4.7885037601725776E-4 0.0 0.0 0.025019932146901718 7.980839600287629E-5 42-43 4.7885037601725776E-4 0.0 0.0 0.03272144236117928 7.980839600287629E-5 44-45 4.7885037601725776E-4 0.0 0.0 0.04054266516946116 7.980839600287629E-5 46-47 4.7885037601725776E-4 0.0 0.0 0.05123699023384658 7.980839600287629E-5 48-49 4.7885037601725776E-4 0.0 0.0 0.0607740935561903 7.980839600287629E-5 50-51 4.7885037601725776E-4 0.0 0.0 0.07438142507468071 7.980839600287629E-5 52-53 4.7885037601725776E-4 0.0 0.0 0.10131675872565146 7.980839600287629E-5 54-55 4.7885037601725776E-4 0.0 0.0 0.1381882376789803 7.980839600287629E-5 56-57 4.7885037601725776E-4 0.0 0.0 0.1778530104924098 7.980839600287629E-5 58-59 4.7885037601725776E-4 0.0 0.0 0.23798863688057711 7.980839600287629E-5 60-61 4.7885037601725776E-4 0.0 0.0 0.30686328263105933 7.980839600287629E-5 62-63 4.7885037601725776E-4 0.0 0.0 0.35622477555883836 7.980839600287629E-5 64-65 4.7885037601725776E-4 0.0 0.0 0.3981640876583499 7.980839600287629E-5 66-67 4.7885037601725776E-4 0.0 0.0 0.5051472425002055 7.980839600287629E-5 68-69 5.586587720201341E-4 0.0 0.0 0.6233833811784668 7.980839600287629E-5 70-71 6.384671680230103E-4 0.0 0.0 0.6742612336303004 1.5961679200575258E-4 72-73 6.384671680230103E-4 0.0 0.0 0.7741813454259014 1.5961679200575258E-4 74-75 6.384671680230103E-4 0.0 0.0 0.9149234517769739 1.5961679200575258E-4 76-77 6.384671680230103E-4 0.0 0.0 1.0597756905221942 1.5961679200575258E-4 78-79 6.384671680230103E-4 0.0 0.0 1.209735666611599 1.5961679200575258E-4 80-81 6.384671680230103E-4 7.980839600287629E-5 0.0 1.3756573219015786 1.5961679200575258E-4 82-83 6.384671680230103E-4 7.980839600287629E-5 0.0 1.5305255143451602 1.5961679200575258E-4 84-85 6.384671680230103E-4 7.980839600287629E-5 0.0 1.6949707143090866 1.5961679200575258E-4 86-87 6.384671680230103E-4 7.980839600287629E-5 0.0 1.9726640282010948 1.5961679200575258E-4 88-89 6.384671680230103E-4 7.980839600287629E-5 0.0 2.3772925959356774 1.5961679200575258E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6160 0.0 26.376287 1 TACATGG 6205 0.0 25.80218 2 ACATGGG 6200 0.0 23.906382 3 TACCTGG 1200 0.0 23.754084 2 GCGTTCG 60 1.7188863E-4 23.735126 34-35 GTACCTG 1260 0.0 22.999985 1 GAGTACT 4900 0.0 22.400734 12-13 CATGGGG 2650 0.0 22.05096 4 GTATCAA 10765 0.0 21.757109 1 GTGTAGC 665 0.0 20.717848 1 GTACTTT 5355 0.0 20.453852 14-15 AGTACTT 5180 0.0 20.043243 12-13 TAGACTG 485 0.0 19.59178 5 TACCGCT 150 7.7105896E-4 19.004025 7 TATCAAC 12405 0.0 18.765821 2 ATCAACG 12345 0.0 18.74083 3 AGAGTAC 9065 0.0 18.687307 10-11 TCAACGC 12390 0.0 18.635166 4 CAACGCA 12405 0.0 18.575077 5 ATGGGCG 155 9.61149E-4 18.390993 5 >>END_MODULE