FastQCFastQC Report
Thu 26 May 2016
SRR936667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253001
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72440.5781320206448359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64370.5137266450705147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52720.4207498637271638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37000.2952910652106423No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23690.18906609013081394No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.15945717521374683No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15570.1242616725764784No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14290.11404619788811023No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12680.10119704613164714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63850.024.8521331
TACATGG66600.023.9667112
GAGTACT74900.023.59133712-13
ACATGGG65550.023.113983
GTACTTT78900.022.18638814-15
TACCTGG11350.022.1831632
GTACCTG11550.021.800781
GTATCAA135100.021.0995881
AGTACTT75850.021.04142412-13
GGTATCA106050.020.9210911
CATGGGG26800.020.2027034
ACTTTTT89250.019.66516716-17
GTGTACA11400.019.5871431
AGAGTAC119700.018.51107410-11
TAAGGTG5150.018.44435
GTGTAGC6200.018.3906751
GTATAGG3901.4551915E-1118.2727831
CATGGGA38550.018.2337824
TACTTTT86800.018.22428914-15
TATCAAC158350.017.970172
Produced by FastQC (version 0.11.5)