##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936667_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253001 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.970030351133 31.0 31.0 34.0 30.0 34.0 2 32.07669028197105 33.0 31.0 34.0 30.0 34.0 3 32.19405810530079 33.0 31.0 34.0 30.0 34.0 4 35.92135920083064 37.0 35.0 37.0 35.0 37.0 5 35.79852530045866 37.0 35.0 37.0 35.0 37.0 6 35.85932014419781 37.0 35.0 37.0 35.0 37.0 7 35.792227619930074 37.0 35.0 37.0 33.0 37.0 8 35.76853170907286 37.0 35.0 37.0 33.0 37.0 9 37.38406992492424 39.0 37.0 39.0 34.0 39.0 10-11 37.282929542753756 39.0 37.0 39.0 34.0 39.0 12-13 37.156123578512705 39.0 37.0 39.0 33.0 39.0 14-15 38.237700129529024 40.0 38.0 41.0 33.0 41.0 16-17 38.22710596400162 40.0 37.5 41.0 33.0 41.0 18-19 38.39975865941049 40.0 38.0 41.0 33.5 41.0 20-21 38.4191856191655 40.0 38.0 41.0 34.0 41.0 22-23 38.330480183176235 40.0 38.0 41.0 34.0 41.0 24-25 38.21039807629842 40.0 38.0 41.0 33.5 41.0 26-27 38.000976056683115 40.0 37.0 41.0 33.0 41.0 28-29 38.00630047382245 40.0 37.0 41.0 33.0 41.0 30-31 37.85644624385775 40.0 37.0 41.0 33.0 41.0 32-33 37.632281618290804 40.0 36.5 41.0 33.0 41.0 34-35 37.42684363380396 40.0 36.0 41.0 32.5 41.0 36-37 37.22496350761092 39.0 36.0 41.0 32.0 41.0 38-39 37.009225850577934 39.0 36.0 41.0 31.0 41.0 40-41 36.76430585450451 39.0 35.0 41.0 31.0 41.0 42-43 36.467498030727825 39.0 35.0 40.5 30.0 41.0 44-45 36.12542487994822 39.0 35.0 40.0 30.0 41.0 46-47 35.661998274542476 38.5 35.0 40.0 28.5 41.0 48-49 35.181470725083216 38.0 35.0 40.0 26.5 41.0 50-51 34.846002916198785 38.0 34.0 40.0 26.0 41.0 52-53 34.57570265307051 38.0 34.0 40.0 24.5 41.0 54-55 34.24581983573836 38.0 34.0 40.0 24.0 41.0 56-57 33.89861700030566 38.0 33.0 40.0 23.0 41.0 58-59 33.520129273639846 37.0 33.0 40.0 22.0 41.0 60-61 33.14160683032176 36.5 32.0 39.0 21.5 40.5 62-63 32.83124913707172 36.0 32.0 39.0 20.0 40.0 64-65 32.51053710252425 36.0 31.5 39.0 19.0 40.0 66-67 32.06801830166137 35.0 31.0 38.5 18.0 40.0 68-69 31.616404136948013 35.0 31.0 38.0 14.5 40.0 70-71 31.103913324889604 35.0 30.0 37.5 10.5 39.5 72-73 30.547393417882347 34.0 29.5 37.0 8.0 39.0 74-75 30.02037149212171 34.0 29.0 36.0 7.0 39.0 76-77 28.67148031007158 32.5 27.0 35.0 6.5 37.0 78-79 29.42484323635815 34.0 29.0 35.0 2.0 37.0 80-81 29.24977513984426 34.0 29.0 35.0 2.0 37.0 82-83 28.846427097823543 34.0 29.0 35.0 2.0 36.5 84-85 28.40097214607171 33.5 28.0 35.0 2.0 36.0 86-87 27.98139666289173 33.0 27.0 35.0 2.0 36.0 88-89 27.48036394224745 33.0 26.0 35.0 2.0 35.0 90-91 26.88921517221455 32.0 25.5 34.0 2.0 35.0 92-93 26.316666147912095 32.0 25.0 34.0 2.0 35.0 94-95 25.57829602689862 32.0 23.5 34.0 2.0 35.0 96-97 24.74313308608692 31.0 19.0 34.0 2.0 35.0 98-99 23.60214556891814 31.0 2.0 34.0 2.0 35.0 100-101 19.873489725866143 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27876471967938343 1101 2 0.40506985154443953 1101 3 0.3233178388015219 1101 4 0.18864090273839906 1101 5 0.24253309526000777 1101 6 0.23560770256709418 1101 7 0.2993593499699472 1101 8 0.2184424694899505 1101 9 0.29700511644725225 1101 10-11 0.2669394211763745 1101 12-13 0.32168760523877893 1101 14-15 0.3911870448954744 1101 16-17 0.4497198442133836 1101 18-19 0.3487564739059792 1101 20-21 0.28867427902265064 1101 22-23 0.29841470592788966 1101 24-25 0.2120049445047556 1101 26-27 0.1437409966249561 1101 28-29 0.2994242746047533 1101 30-31 0.4582487981442469 1101 32-33 0.47316794930240036 1101 34-35 0.40683088124741573 1101 36-37 0.5260698986493537 1101 38-39 0.4873600238771516 1101 40-41 0.6205878071441688 1101 42-43 0.6874313119405215 1101 44-45 0.6752916977172774 1101 46-47 0.6808186723271916 1101 48-49 0.749216805076081 1101 50-51 0.8376157898377627 1101 52-53 0.7630971093596628 1101 54-55 1.007861127252042 1101 56-57 0.9593079901287425 1101 58-59 0.7152778877240777 1101 60-61 0.7435583768204097 1101 62-63 0.6415825135769495 1101 64-65 0.796933314959297 1101 66-67 0.7452319446930957 1101 68-69 0.6496430143097705 1101 70-71 0.6613302844020907 1101 72-73 0.8704013944941202 1101 74-75 0.778016367035093 1101 76-77 0.9400035042910524 1101 78-79 0.7485928945964382 1101 80-81 0.6348459374642701 1101 82-83 0.6688843630128076 1101 84-85 0.8123150337587717 1101 86-87 0.784630413377922 1101 88-89 0.6467556049859233 1101 90-91 0.761990941287312 1101 92-93 0.7737272370085222 1101 94-95 0.40577069813063105 1101 96-97 0.4363501931855289 1101 98-99 0.12487535599404964 1101 100-101 0.040333714989728264 1102 1 0.21798214938158011 1102 2 0.4132521134143019 1102 3 0.2792079203017721 1102 4 0.1660980740628446 1102 5 0.12814261529246096 1102 6 0.16736445983040227 1102 7 0.23901198244065114 1102 8 0.09176285294819309 1102 9 0.17477498902017885 1102 10-11 0.23624375648567764 1102 12-13 0.21165702484096727 1102 14-15 0.341051573508274 1102 16-17 0.34371929393115863 1102 18-19 0.3516214650857137 1102 20-21 0.2529479177301326 1102 22-23 0.2364629733260628 1102 24-25 0.18541071916942542 1102 26-27 0.29264444308601867 1102 28-29 0.34906569151402067 1102 30-31 0.4131950031835956 1102 32-33 0.3592242925045852 1102 34-35 0.4445059088552483 1102 36-37 0.4996445439834645 1102 38-39 0.4442436496822353 1102 40-41 0.49176085291130534 1102 42-43 0.4725650327704969 1102 44-45 0.5873005202257744 1102 46-47 0.5321251427915001 1102 48-49 0.5501872585191379 1102 50-51 0.5960124668528337 1102 52-53 0.5708997964462483 1102 54-55 0.6008104675506232 1102 56-57 0.6132899267773126 1102 58-59 0.6053230273600647 1102 60-61 0.4229560052089667 1102 62-63 0.37837255391848146 1102 64-65 0.20525434691089828 1102 66-67 0.4452572846647982 1102 68-69 0.5131241041684653 1102 70-71 0.2613472651819464 1102 72-73 0.2223336797922144 1102 74-75 0.581690625447326 1102 76-77 0.556668396659898 1102 78-79 0.41129631721693727 1102 80-81 0.5824441129863089 1102 82-83 0.6473675033100363 1102 84-85 0.5435507097400034 1102 86-87 0.6624750688555174 1102 88-89 0.3649277008789724 1102 90-91 0.2637801082244984 1102 92-93 0.4946824189952501 1102 94-95 0.6201789085813445 1102 96-97 0.8112344577378288 1102 98-99 0.4890682891407536 1102 100-101 0.21640640922098342 1103 1 0.23114120609507083 1103 2 0.30057284782213145 1103 3 0.24784737631078002 1103 4 0.12565115767263535 1103 5 0.2835544491959183 1103 6 0.1613564485933452 1103 7 0.2365013893791641 1103 8 0.17098712259763715 1103 9 0.20285764072632162 1103 10-11 0.21932318403546702 1103 12-13 0.2694702303855223 1103 14-15 0.34250723427704344 1103 16-17 0.39578442022310867 1103 18-19 0.12485788488920235 1103 20-21 0.2928558084987145 1103 22-23 0.26625767704525316 1103 24-25 0.3745910086603459 1103 26-27 0.31560614310784985 1103 28-29 0.4323361940658472 1103 30-31 0.4689439572072942 1103 32-33 0.4563549183241591 1103 34-35 0.3404191865475781 1103 36-37 0.4881321397292453 1103 38-39 0.44402516518567836 1103 40-41 0.5170504854574531 1103 42-43 0.5787018436383207 1103 44-45 0.5746609109235408 1103 46-47 0.555727716784645 1103 48-49 0.6438781594000673 1103 50-51 0.758516403322794 1103 52-53 0.7718547132430231 1103 54-55 0.7301447500800222 1103 56-57 0.7530535636256701 1103 58-59 0.7233511016806133 1103 60-61 0.5001791318241757 1103 62-63 0.7498907566554323 1103 64-65 0.922379199241206 1103 66-67 0.6183315802241296 1103 68-69 0.6446178216457206 1103 70-71 0.5102583834393357 1103 72-73 0.5558120446200832 1103 74-75 0.8063726669797937 1103 76-77 0.8277174721518143 1103 78-79 0.7767992189576454 1103 80-81 0.9329157395773535 1103 82-83 0.8819523702698469 1103 84-85 0.9314081787022914 1103 86-87 0.8206872116781447 1103 88-89 0.6079289072299758 1103 90-91 0.6193783100283561 1103 92-93 0.5159620591560774 1103 94-95 0.5165266559207886 1103 96-97 0.7255160540534611 1103 98-99 0.5627446354967915 1103 100-101 0.28502595377349493 1104 1 0.13309597359592473 1104 2 0.21494299201663836 1104 3 0.27237269727608293 1104 4 0.21970819325859736 1104 5 0.07369339957241294 1104 6 0.13366185061461522 1104 7 0.09926342949674449 1104 8 0.07161660789412849 1104 9 0.1580811526576582 1104 10-11 0.13910038462144314 1104 12-13 0.2470743861665099 1104 14-15 0.29009374812932975 1104 16-17 0.2636879852797449 1104 18-19 0.2140873781658854 1104 20-21 0.1564771444971811 1104 22-23 0.11897844815999292 1104 24-25 0.15802445859318226 1104 26-27 0.30366744049737804 1104 28-29 0.31247249387793374 1104 30-31 0.3459979071182815 1104 32-33 0.24210689959730303 1104 34-35 0.3577402449492979 1104 36-37 0.5004686645067977 1104 38-39 0.6741585199262019 1104 40-41 0.4379586885267912 1104 42-43 0.6053314657381392 1104 44-45 0.7579987410328144 1104 46-47 0.5871996065515646 1104 48-49 0.5285610321938847 1104 50-51 0.5336742021573002 1104 52-53 0.67020984327268 1104 54-55 0.46870385956638216 1104 56-57 0.6028009999883679 1104 58-59 0.6237580440693122 1104 60-61 0.3477650281614544 1104 62-63 0.41403812043637345 1104 64-65 0.4940242402157011 1104 66-67 0.6030281333503709 1104 68-69 0.48928120946604636 1104 70-71 0.3134520760460653 1104 72-73 0.5621959622492838 1104 74-75 0.6345970928333422 1104 76-77 0.4206990816049192 1104 78-79 0.6942253499126316 1104 80-81 0.6643489634046915 1104 82-83 0.5791405869435131 1104 84-85 0.3677088933096542 1104 86-87 0.6185975884002985 1104 88-89 0.67290739870732 1104 90-91 0.8035312599450428 1104 92-93 0.8685025633903258 1104 94-95 0.9687706579500421 1104 96-97 1.0595242204519053 1104 98-99 0.8803266652401156 1104 100-101 0.4080254426610743 1105 1 0.1695237235789797 1105 2 0.26628322409131755 1105 3 0.14060330513900965 1105 4 0.10993004959337327 1105 5 0.13650182159171464 1105 6 0.1462558974950241 1105 7 0.2501382408250805 1105 8 0.21679804002828007 1105 9 0.17905687081831445 1105 10-11 0.2618668615087998 1105 12-13 0.23061312841146986 1105 14-15 0.3618848588505017 1105 16-17 0.31131861594350596 1105 18-19 0.290866565731136 1105 20-21 0.22493140018911362 1105 22-23 0.04010962178835342 1105 24-25 0.190240621023392 1105 26-27 0.273632593044951 1105 28-29 0.23406625128229308 1105 30-31 0.26057567924546277 1105 32-33 0.31164813522398305 1105 34-35 0.10882945768477725 1105 36-37 0.36476849234472297 1105 38-39 0.41236657387486986 1105 40-41 0.3853975084732113 1105 42-43 0.4421410509152821 1105 44-45 0.36872989658862565 1105 46-47 0.3878866787403723 1105 48-49 0.2562029476952361 1105 50-51 0.3061778127274337 1105 52-53 0.2912683589382681 1105 54-55 0.24979613517910337 1105 56-57 0.38382340207218135 1105 58-59 0.5068927281858038 1105 60-61 0.35532056316940697 1105 62-63 0.4815850709822058 1105 64-65 0.33016214639499 1105 66-67 0.3311428772428684 1105 68-69 0.21523039932713672 1105 70-71 -0.022607288083808186 1105 72-73 0.10520942367357833 1105 74-75 0.29913664466557677 1105 76-77 0.12252824450428434 1105 78-79 0.0771892904434246 1105 80-81 0.17366998708866532 1105 82-83 0.3157236873213023 1105 84-85 0.2534730286457503 1105 86-87 0.5019899639899528 1105 88-89 0.4501476990268998 1105 90-91 0.48654185287709595 1105 92-93 0.372080035534168 1105 94-95 0.4734049015480757 1105 96-97 0.5444247084633425 1105 98-99 0.5461066035517703 1105 100-101 0.26744069053610176 1106 1 -0.011098451161931422 1106 2 0.12918820817539256 1106 3 0.035582398827386896 1106 4 0.06436131555169311 1106 5 0.11538959612332178 1106 6 0.07643293495196701 1106 7 0.026117906227192123 1106 8 0.03108192012344091 1106 9 0.19763151095358467 1106 10-11 0.11805542045103579 1106 12-13 0.1937778667555321 1106 14-15 0.19453504271945832 1106 16-17 0.10741091621918741 1106 18-19 0.09424034296626616 1106 20-21 -0.025633651628503173 1106 22-23 0.015013569035652097 1106 24-25 0.2554238601208567 1106 26-27 0.1637351623652421 1106 28-29 0.17029594454334074 1106 30-31 0.07350597861996988 1106 32-33 0.03726793589937216 1106 34-35 0.061521054461856295 1106 36-37 0.07361418777634299 1106 38-39 0.06156599295557896 1106 40-41 0.14176902792359414 1106 42-43 0.3288726894565386 1106 44-45 0.37115725745142214 1106 46-47 0.05787643149841415 1106 48-49 0.30222923441452565 1106 50-51 0.38349997584027307 1106 52-53 0.4183002440916397 1106 54-55 0.2618224122083248 1106 56-57 0.38040159846069344 1106 58-59 0.447843601987266 1106 60-61 0.36865742126327206 1106 62-63 0.29239960559268496 1106 64-65 0.30437840196072585 1106 66-67 -0.008196175650340365 1106 68-69 0.09918159532320203 1106 70-71 0.06294022471918836 1106 72-73 0.10727825219337106 1106 74-75 0.15627694951497162 1106 76-77 0.0893097989842424 1106 78-79 -0.0811260557098592 1106 80-81 0.053321520298936775 1106 82-83 0.19380512745851775 1106 84-85 0.39215963746273985 1106 86-87 0.3711765316241724 1106 88-89 0.2849339362299581 1106 90-91 0.4148835317774662 1106 92-93 0.5140455195286044 1106 94-95 0.570328851508588 1106 96-97 0.7524921706842065 1106 98-99 0.8026483047392858 1106 100-101 0.7314121266519393 1107 1 0.049348928782603 1107 2 0.19754805831892241 1107 3 0.024035017261027747 1107 4 0.10041039192335433 1107 5 0.04291842403165447 1107 6 0.06724936077787902 1107 7 -0.02756647074940588 1107 8 0.07321283369647347 1107 9 0.0697629110736031 1107 10-11 0.12157883146969795 1107 12-13 0.20697646712206108 1107 14-15 0.10605647511375338 1107 16-17 0.18184550808736333 1107 18-19 0.3208161469851447 1107 20-21 0.19785562001067802 1107 22-23 0.1286425607481405 1107 24-25 0.1404791642938079 1107 26-27 0.2559682508032637 1107 28-29 0.23535538436559023 1107 30-31 0.26706855667021756 1107 32-33 0.13866027116268498 1107 34-35 0.23158037264736464 1107 36-37 0.21482836575522413 1107 38-39 0.3280609490852555 1107 40-41 -0.0015383156921018326 1107 42-43 0.07167179421011127 1107 44-45 -0.04377000377564144 1107 46-47 -0.15305992815711988 1107 48-49 0.05628277890924238 1107 50-51 -0.024509195570225017 1107 52-53 0.10424228012855252 1107 54-55 0.11901580652221355 1107 56-57 0.15620480642709111 1107 58-59 0.1532870490104088 1107 60-61 0.09921074681185615 1107 62-63 -0.23948239865649157 1107 64-65 -0.09429312206659546 1107 66-67 -0.062333649498441446 1107 68-69 0.15226184313779356 1107 70-71 -0.12826868233412014 1107 72-73 -0.09970694056032414 1107 74-75 0.008629201416557208 1107 76-77 -0.16405447062765433 1107 78-79 -0.053754747385038826 1107 80-81 0.04963085875722939 1107 82-83 -0.022337284841391636 1107 84-85 0.11173828778142791 1107 86-87 0.052155788504759215 1107 88-89 0.0968571753353622 1107 90-91 0.17289837956935727 1107 92-93 0.0803659269306074 1107 94-95 0.3141750459003241 1107 96-97 0.36159222096072696 1107 98-99 0.5098352117601621 1107 100-101 0.1829652251452636 1108 1 -0.06889287600438365 1108 2 0.22756095028924506 1108 3 -0.011257584554805078 1108 4 0.0017183234100457412 1108 5 0.0656678273800182 1108 6 0.1535433673075417 1108 7 0.042484837959598565 1108 8 0.024945326422994185 1108 9 -0.012781242628896905 1108 10-11 -0.013917183571173553 1108 12-13 0.05130759156697451 1108 14-15 -0.02300318116454747 1108 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-1.001159086420877 2316 62-63 -1.1368973384101366 2316 64-65 -1.0597688335100415 2316 66-67 -1.2782180357457307 2316 68-69 -1.539362279433906 2316 70-71 -1.1982851550723659 2316 72-73 -1.0244674641111686 2316 74-75 -1.4310116258250751 2316 76-77 -1.4294230883148202 2316 78-79 -1.6726983265100692 2316 80-81 -1.4080230963765636 2316 82-83 -1.4350038285563222 2316 84-85 -1.3294719205658794 2316 86-87 -1.2852335549329723 2316 88-89 -1.293916973503304 2316 90-91 -1.8515805425910195 2316 92-93 -1.8656135513069323 2316 94-95 -2.2741304975463486 2316 96-97 -2.2553299897114805 2316 98-99 -1.5927875723142364 2316 100-101 -1.2222980173155555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 16.0 5 38.0 6 108.0 7 227.0 8 477.0 9 957.0 10 1647.0 11 2736.0 12 4023.0 13 5605.0 14 7272.0 15 10065.0 16 12930.0 17 16977.0 18 15180.0 19 9566.0 20 8363.0 21 8651.0 22 9152.0 23 10174.0 24 11663.0 25 13929.0 26 16606.0 27 20250.0 28 25342.0 29 30849.0 30 38096.0 31 47470.0 32 59264.0 33 76291.0 34 100676.0 35 137621.0 36 191588.0 37 235146.0 38 119369.0 39 4666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.047176242024086 19.368647402608804 14.93150728667964 21.652669068687473 2 15.774224522357812 21.081067274975197 39.527230613158714 23.617477589508272 3 20.30054304623076 24.997605616266487 30.06084927194772 24.641002065555035 4 13.067969145926362 17.16591047849353 36.98753785928914 32.77858251629097 5 13.30585623047564 37.84369657488654 33.89237318843472 14.9580740062031 6 31.28281621483143 36.66621175880945 16.94946771790286 15.10150430845626 7 28.288974355086243 33.519314092531744 20.346098194967745 17.845613357414265 8 26.865846748126454 34.13768885128934 19.191441135861194 19.80502326472302 9 26.459994844377615 17.11650669073688 20.61929719130312 35.80420127358238 10-11 25.272645432844826 26.922644116006296 26.445788949889103 21.358921501259776 12-13 26.84135926467736 24.297785875669693 27.487966889092668 21.372887970560278 14-15 23.01396615385475 25.64490558011674 26.80273281545355 24.538395450574964 16-17 21.517619415516833 29.050725655580173 27.428735386324597 22.002919542578393 18-19 21.38555647204501 27.0331400724439 30.674449777109498 20.90685367840159 20-21 22.271078495923767 25.692879859855783 31.041544459475094 20.99449718474535 22-23 21.88551355531604 25.660626873759725 31.06778915008075 21.386070420843488 24-25 22.076894928992164 25.836964298754467 31.182594031662525 20.903546740590848 26-27 21.692138866719873 25.97873104549082 31.290103750997606 21.0390263367917 28-29 21.165226524160794 26.609475970091008 31.18728556481599 21.03801194093221 30-31 21.434930480540817 25.981279247434845 31.11470162079722 21.469088651227118 32-33 21.33125931790538 26.23758543741081 31.129226378411513 21.301928866272295 34-35 22.13181643146308 26.054625227206067 30.681229137444955 21.132329203885895 36-37 22.00010774292538 25.75155678550977 30.978086286121314 21.270249185443532 38-39 21.577355120394305 26.231308544545634 30.938496526784288 21.252839808275777 40-41 21.77736561158595 26.34093122867868 30.622014915397084 21.25968824433829 42-43 21.63520762726575 26.562147714426064 30.137109295969566 21.66553536233862 44-45 21.859094540633773 26.393335200983948 29.99207445700453 21.75549580137775 46-47 22.427954965718303 26.87072875440642 29.306999754988222 21.39431652488705 48-49 22.557577334438975 27.031517988901545 28.88495866211959 21.525946014539887 50-51 22.12321436694874 27.349126527250483 29.049862448692604 21.477796657108176 52-53 22.510758249055062 27.541955575135358 28.443397882827664 21.50388829298192 54-55 22.015651243276633 27.275310165726324 28.6340098300119 22.07502876098514 56-57 22.298533957946375 27.506547182416753 28.37857361220087 21.816345247436004 58-59 22.927002126151667 27.059629615441093 28.19920381047241 21.81416444793482 60-61 22.20351090023185 27.306367746646927 29.073669844487632 21.416451508633592 62-63 22.12406648856043 27.252927479903192 29.07962705432373 21.543378977212644 64-65 21.94506232107184 27.61748900791494 28.754248982598625 21.683199688414597 66-67 22.005956960481665 27.526161463637322 28.363240509885166 22.104641065995846 68-69 21.95552437651564 27.442450129830643 28.64789778914802 21.954127704505705 70-71 22.332040101106763 27.46403838034436 28.28770572728151 21.916215791267373 72-73 22.366591697407255 27.212165132430616 28.587470609549566 21.833772560612566 74-75 21.988463398787754 28.13521145646597 28.08911773016134 21.787207414584934 76-77 22.24550003332052 27.50607251583523 28.1330570323664 22.11537041847785 78-79 22.360001564384394 27.534242857906804 28.06218268338217 22.043572894326633 80-81 22.352868407610778 27.744221683054526 27.833091239943812 22.069818669390884 82-83 22.270450125023643 27.756406214708658 28.075330237205726 21.897813423061972 84-85 22.139397388144573 27.926126455431415 27.773609932808128 22.16086622361588 86-87 21.99528089376504 27.92500986638776 27.740970309854458 22.33873892999274 88-89 22.046377806332153 27.97716625298291 27.406442293111567 22.57001364757337 90-91 22.06101785823319 27.69776090663587 27.621298025254298 22.619923209876642 92-93 22.274936929905 27.543734052903062 27.820516197102723 22.360812820089212 94-95 22.083833635210397 27.356766016743105 27.86291389380711 22.696486454239388 96-97 21.885532231071213 27.283062476506366 28.17979104532406 22.65161424709836 98-99 21.78869529442139 27.646356739052102 27.543363223134392 23.021584743392115 100-101 21.400483968542044 27.927913907231915 27.556078577847586 23.115523546378455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 341.0 1 263.5 2 241.5 3 333.5 4 306.5 5 283.5 6 467.0 7 914.0 8 1482.0 9 1858.0 10 1866.5 11 1745.5 12 1778.0 13 1929.0 14 2094.5 15 2207.0 16 2406.0 17 2645.0 18 2867.0 19 3100.0 20 3317.5 21 3720.0 22 4204.0 23 4794.0 24 5612.0 25 6623.0 26 8143.5 27 10100.5 28 12018.5 29 14096.5 30 16526.5 31 19230.5 32 21789.5 33 24038.0 34 26464.5 35 28271.0 36 30528.5 37 33027.0 38 34323.5 39 34978.0 40 35712.0 41 37583.5 42 39359.0 43 40688.0 44 41944.5 45 43294.5 46 44144.5 47 47420.0 48 56102.0 49 55230.5 50 46677.5 51 42374.0 52 39728.5 53 38843.0 54 37330.0 55 34375.0 56 31379.0 57 28509.5 58 26062.5 59 23132.5 60 19590.5 61 16376.0 62 13537.5 63 10648.0 64 7864.5 65 6048.5 66 4621.5 67 3585.0 68 2640.5 69 1718.5 70 1241.5 71 970.5 72 797.5 73 704.0 74 563.0 75 452.0 76 344.5 77 225.5 78 142.5 79 94.5 80 69.0 81 42.5 82 22.5 83 17.0 84 12.5 85 6.5 86 3.5 87 1.5 88 0.0 89 0.0 90 0.5 91 1.0 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013328002132480341 2 0.010215474688368167 3 0.005506779324198465 4 4.788503760172578E-4 5 0.006304863284227228 6 0.0 7 3.192335840115052E-4 8 0.002474060276089165 9 0.0 10-11 0.0 12-13 0.0 14-15 5.985629700215721E-4 16-17 0.005187545740186959 18-19 0.002513964474090603 20-21 4.788503760172578E-4 22-23 4.389461780158196E-4 24-25 4.389461780158196E-4 26-27 7.98083960028763E-5 28-29 0.0 30-31 5.187545740186959E-4 32-33 0.0029529106521064227 34-35 6.783713660244485E-4 36-37 0.0014764553260532113 38-39 0.004429365978159634 40-41 3.990419800143815E-5 42-43 0.0017956889100647167 44-45 0.007182755640258867 46-47 0.0 48-49 0.002713485464097794 50-51 0.0011173175440402682 52-53 7.182755640258866E-4 54-55 1.1971259400431445E-4 56-57 0.013727044112494723 58-59 0.002633677068094918 60-61 0.0036711862161323094 62-63 2.7932938601006705E-4 64-65 0.00430965338415532 66-67 0.0010375091480373919 68-69 0.0017956889100647167 70-71 0.005426970928195588 72-73 0.0034317610281236807 74-75 0.009497199124342279 76-77 0.0015562637220560879 78-79 0.008938540352322145 80-81 0.0032721442361179284 82-83 0.0030327190481092992 84-85 0.0017557847120632784 86-87 7.581797620273248E-4 88-89 0.002474060276089165 90-91 0.00841978577830345 92-93 0.0023942518800862886 94-95 5.985629700215721E-4 96-97 1.596167920057526E-4 98-99 7.581797620273248E-4 100-101 0.00215482669207766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.85084657046653 #Duplication Level Percentage of deduplicated Percentage of total 1 81.66580138653221 49.69433150226064 2 11.024510052425377 13.41701539429405 3 3.1788276503656427 5.8030306087906895 4 1.3308284937157648 3.2392816193081235 5 0.700427691252288 2.131080898704954 6 0.4249483311845313 1.5515079420771427 7 0.2939049776518335 1.251905669097168 8 0.19891455549618164 0.9683295277704559 9 0.17211009017009246 0.942574022115251 >10 0.901955267578419 10.162192020611077 >50 0.0660182182158102 2.748884112423667 >100 0.03794560893835428 4.374591728935821 >500 0.0019694878311690873 0.7481523981336402 >1k 0.0014442910761906641 1.452841008934442 >5k 3.938975662338175E-4 1.5142815465428112 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7244 0.5781320206448359 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6437 0.5137266450705147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5272 0.4207498637271638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3700 0.2952910652106423 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2369 0.18906609013081394 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1998 0.15945717521374683 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1557 0.1242616725764784 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1429 0.11404619788811023 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1268 0.10119704613164714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.9952099000719072E-4 0.0 0.0 0.0 0.0 16-17 3.1923358401150516E-4 0.0 0.0 3.9904198001438144E-5 0.0 18-19 3.1923358401150516E-4 0.0 0.0 2.3942518800862888E-4 0.0 20-21 3.990419800143815E-4 0.0 0.0 2.3942518800862888E-4 3.9904198001438144E-5 22-23 3.990419800143815E-4 0.0 0.0 4.7885037601725776E-4 7.980839600287629E-5 24-25 3.990419800143815E-4 0.0 0.0 6.783713660244485E-4 7.980839600287629E-5 26-27 3.990419800143815E-4 0.0 0.0 9.177965540330775E-4 7.980839600287629E-5 28-29 3.990419800143815E-4 0.0 0.0 0.001915401504069031 7.980839600287629E-5 30-31 3.990419800143815E-4 0.0 0.0 0.003910611404140938 7.980839600287629E-5 32-33 3.990419800143815E-4 0.0 0.0 0.008180360590294821 7.980839600287629E-5 34-35 4.389461780158196E-4 0.0 0.0 0.011811642608425692 7.980839600287629E-5 36-37 4.7885037601725776E-4 0.0 0.0 0.01504388264654218 7.980839600287629E-5 38-39 4.7885037601725776E-4 0.0 0.0 0.019712673812710448 7.980839600287629E-5 40-41 4.7885037601725776E-4 0.0 0.0 0.025937728700934796 7.980839600287629E-5 42-43 4.7885037601725776E-4 0.0 0.0 0.03387866410322099 7.980839600287629E-5 44-45 4.7885037601725776E-4 0.0 0.0 0.04173979110950431 7.980839600287629E-5 46-47 4.7885037601725776E-4 0.0 0.0 0.052234595183882536 7.980839600287629E-5 48-49 4.7885037601725776E-4 0.0 0.0 0.0616120817142205 7.980839600287629E-5 50-51 4.7885037601725776E-4 0.0 0.0 0.07533912582671523 7.980839600287629E-5 52-53 5.586587720201341E-4 0.0 0.0 0.10175570490366728 7.980839600287629E-5 54-55 5.586587720201341E-4 0.0 0.0 0.13802862088697454 7.980839600287629E-5 56-57 5.586587720201341E-4 0.0 0.0 0.17765348950240262 7.980839600287629E-5 58-59 5.586587720201341E-4 0.0 0.0 0.23790882848457423 7.980839600287629E-5 60-61 5.586587720201341E-4 0.0 0.0 0.3074618456010809 7.980839600287629E-5 62-63 5.586587720201341E-4 0.0 0.0 0.35674353013285703 7.980839600287629E-5 64-65 5.586587720201341E-4 0.0 0.0 0.39840351284635844 7.980839600287629E-5 66-67 5.586587720201341E-4 0.0 0.0 0.5050275299062013 7.980839600287629E-5 68-69 5.586587720201341E-4 0.0 0.0 0.623223764386461 7.980839600287629E-5 70-71 5.586587720201341E-4 0.0 0.0 0.6739819042442903 7.980839600287629E-5 72-73 5.586587720201341E-4 0.0 0.0 0.7738222076438885 7.980839600287629E-5 74-75 5.586587720201341E-4 0.0 0.0 0.9143248888069522 7.980839600287629E-5 76-77 5.586587720201341E-4 0.0 0.0 1.0591771275521729 7.980839600287629E-5 78-79 5.586587720201341E-4 0.0 0.0 1.2084986364735544 7.980839600287629E-5 80-81 6.384671680230103E-4 0.0 0.0 1.3739015371895154 7.980839600287629E-5 82-83 6.384671680230103E-4 0.0 0.0 1.528889442227101 7.980839600287629E-5 84-85 6.384671680230103E-4 0.0 0.0 1.6913793364889573 7.980839600287629E-5 86-87 6.384671680230103E-4 0.0 0.0 1.9652817515708287 7.980839600287629E-5 88-89 6.384671680230103E-4 0.0 0.0 2.367037217049308 7.980839600287629E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6385 0.0 24.852133 1 TACATGG 6660 0.0 23.966711 2 GAGTACT 7490 0.0 23.591337 12-13 ACATGGG 6555 0.0 23.11398 3 GTACTTT 7890 0.0 22.186388 14-15 TACCTGG 1135 0.0 22.183163 2 GTACCTG 1155 0.0 21.80078 1 GTATCAA 13510 0.0 21.099588 1 AGTACTT 7585 0.0 21.041424 12-13 GGTATCA 10605 0.0 20.921091 1 CATGGGG 2680 0.0 20.202703 4 ACTTTTT 8925 0.0 19.665167 16-17 GTGTACA 1140 0.0 19.587143 1 AGAGTAC 11970 0.0 18.511074 10-11 TAAGGTG 515 0.0 18.4443 5 GTGTAGC 620 0.0 18.390675 1 GTATAGG 390 1.4551915E-11 18.272783 1 CATGGGA 3855 0.0 18.233782 4 TACTTTT 8680 0.0 18.224289 14-15 TATCAAC 15835 0.0 17.97017 2 >>END_MODULE