FastQCFastQC Report
Thu 26 May 2016
SRR936666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265803
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53590.4233676172358574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42670.3370982688459421No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40610.3208240144793463No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26490.20927427095685505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25480.20129514624313577No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16140.1275079929499298No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16040.12671798060203682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63300.026.1289021
TACATGG63900.024.989052
ACATGGG63050.024.4214383
GAGTACT51250.022.99189212-13
GTATCAA110300.022.9666351
GGACCGT1050.002188947522.632186
GTACTTT54950.021.22591214-15
AGTACTT52950.021.08719412-13
CATGGGA39050.020.4463964
CATGGGG24450.020.4098074
TCAACGC124700.019.97074
ATCAACG125300.019.8742873
CAACGCA126150.019.7042585
AGAGTAC90900.019.68240210-11
GTACCTG11400.019.59471
TATCAAC128400.019.468482
AACGCAG128100.019.4414126
ATGGGAG17350.019.1753925
GGTATCA84500.019.0110281
TACCTGG10550.018.4689712
Produced by FastQC (version 0.11.5)