##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936666_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1265803 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.522909173070374 31.0 31.0 34.0 30.0 34.0 2 31.575915051552254 33.0 31.0 34.0 30.0 34.0 3 31.62380481006918 33.0 31.0 34.0 30.0 34.0 4 35.25549315335799 37.0 35.0 37.0 33.0 37.0 5 35.192386176995946 37.0 35.0 37.0 33.0 37.0 6 35.30823042764158 37.0 35.0 37.0 33.0 37.0 7 35.16042148738785 37.0 35.0 37.0 32.0 37.0 8 35.15779074626936 37.0 35.0 37.0 32.0 37.0 9 36.68873513493016 39.0 37.0 39.0 32.0 39.0 10-11 36.568812445538526 39.0 37.0 39.0 32.0 39.0 12-13 36.47870719219341 39.0 37.0 39.0 32.0 39.0 14-15 37.58615479659947 40.0 37.0 41.0 32.0 41.0 16-17 37.5364796101763 40.0 37.0 41.0 32.0 41.0 18-19 37.51224163633678 40.0 37.0 41.0 32.0 41.0 20-21 37.42780037651988 40.0 37.0 41.0 32.0 41.0 22-23 37.331358434132326 39.5 37.0 41.0 32.0 41.0 24-25 37.18392356472532 39.0 36.5 41.0 31.0 41.0 26-27 36.990815316443395 39.0 36.0 41.0 31.0 41.0 28-29 37.10486860909636 39.0 36.0 41.0 31.0 41.0 30-31 37.03658468181858 39.0 36.0 41.0 31.0 41.0 32-33 36.875764633201214 39.0 36.0 41.0 30.5 41.0 34-35 36.704086654874416 39.0 35.5 41.0 30.0 41.0 36-37 36.472186825280076 39.0 35.0 41.0 30.0 41.0 38-39 36.295697276748434 39.0 35.0 40.0 30.0 41.0 40-41 36.14886716179374 39.0 35.0 40.0 30.0 41.0 42-43 35.87725973156961 38.5 35.0 40.0 29.0 41.0 44-45 35.59265304316706 38.0 35.0 40.0 27.5 41.0 46-47 35.228335688886816 38.0 34.5 40.0 26.5 41.0 48-49 34.96218882401132 38.0 34.0 40.0 25.0 41.0 50-51 34.26140402574492 37.5 33.5 39.5 24.5 40.5 52-53 34.439806984183164 38.0 33.5 39.5 24.5 40.5 54-55 34.7938071722061 38.0 34.0 40.0 25.0 41.0 56-57 34.71048180483061 38.0 34.0 40.0 25.0 41.0 58-59 34.49161362392094 38.0 34.0 40.0 24.0 41.0 60-61 34.169574965456704 38.0 34.0 40.0 23.0 41.0 62-63 33.8621136148358 37.0 33.0 40.0 23.0 41.0 64-65 33.55697055544978 37.0 33.0 40.0 22.0 41.0 66-67 33.182790292012264 36.0 33.0 39.0 21.5 41.0 68-69 32.80137825554213 36.0 32.0 39.0 20.5 41.0 70-71 32.39734461049626 35.0 32.0 38.5 19.5 40.0 72-73 31.987379947748583 35.0 31.5 38.0 18.0 40.0 74-75 31.572742361963115 35.0 31.0 37.0 17.5 39.0 76-77 31.154405938364818 35.0 31.0 37.0 13.5 39.0 78-79 30.709435828482 35.0 30.5 36.0 9.5 39.0 80-81 30.283039303904317 34.0 30.0 36.0 7.0 37.5 82-83 29.879077549982107 34.0 30.0 35.0 4.5 37.0 84-85 29.499403540677342 34.0 29.0 35.0 2.0 37.0 86-87 29.172578987409572 34.0 29.0 35.0 2.0 36.0 88-89 28.79321940301927 34.0 29.0 35.0 2.0 36.0 90-91 28.437692121127853 34.0 29.0 35.0 2.0 35.5 92-93 28.08298210701033 34.0 28.5 35.0 2.0 35.0 94-95 27.671522345894267 34.0 27.0 35.0 2.0 35.0 96-97 27.11144585689874 33.5 26.0 35.0 2.0 35.0 98-99 26.48062850222349 33.0 25.0 35.0 2.0 35.0 100-101 24.393847620838315 31.0 13.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1608405061592606 1101 2 0.2204533428836939 1101 3 0.2607389072449706 1101 4 0.3187200697760417 1101 5 0.24679706417747838 1101 6 0.29100758148480566 1101 7 0.1627925819432079 1101 8 0.26523815755389535 1101 9 0.4956782057761515 1101 10-11 0.3621373506047689 1101 12-13 0.4321006284173805 1101 14-15 0.2990530973162251 1101 16-17 0.4457133754438942 1101 18-19 0.4778622311974061 1101 20-21 0.5642774527364622 1101 22-23 0.42078017738526796 1101 24-25 0.4539494643253832 1101 26-27 0.3968928243188614 1101 28-29 0.4222410002574435 1101 30-31 0.41856910589241636 1101 32-33 0.26201807082902207 1101 34-35 0.10410712406117284 1101 36-37 0.05057469633549516 1101 38-39 0.07324099109728621 1101 40-41 0.029089515160755752 1101 42-43 -0.00473565292356426 1101 44-45 0.19131065982987394 1101 46-47 0.16903549072579693 1101 48-49 0.08454024487046752 1101 50-51 0.29504768188549946 1101 52-53 0.30969754962764995 1101 54-55 0.32946956778431513 1101 56-57 0.2607827475331277 1101 58-59 0.2785688615616948 1101 60-61 0.28603367672496205 1101 62-63 0.41211070652412474 1101 64-65 0.3854959662046724 1101 66-67 0.42019111431154954 1101 68-69 0.4386979752367992 1101 70-71 0.3350114696121622 1101 72-73 0.4263537585564663 1101 74-75 0.3949967068787785 1101 76-77 0.3452000664110386 1101 78-79 0.24155584279330355 1101 80-81 0.3476832413978599 1101 82-83 0.22223855447285246 1101 84-85 0.32578429874183357 1101 86-87 0.29080113244081573 1101 88-89 0.3368878554581656 1101 90-91 0.35853010036351307 1101 92-93 0.22773991632815083 1101 94-95 0.1970109824267965 1101 96-97 -0.06878776486680138 1101 98-99 0.09003723780456596 1101 100-101 0.3679389744304551 1102 1 0.17669142194268872 1102 2 0.12205916665336858 1102 3 0.09989963866596341 1102 4 0.08309620330609135 1102 5 0.17349888737107477 1102 6 0.11461849954575598 1102 7 0.09490587549797169 1102 8 0.06948172563435406 1102 9 0.3012946535378944 1102 10-11 0.2912546917933412 1102 12-13 0.30875179338902825 1102 14-15 0.3521236554274836 1102 16-17 0.3245412530402305 1102 18-19 0.19811731418477763 1102 20-21 -0.004462656350369798 1102 22-23 -0.12967959831184572 1102 24-25 0.02902159995879572 1102 26-27 0.22169642950961332 1102 28-29 0.36410093571635116 1102 30-31 0.16689303047279225 1102 32-33 0.2305299248529593 1102 34-35 0.037987007974301434 1102 36-37 -0.09910251449816343 1102 38-39 -0.1251021082480932 1102 40-41 -0.2609508272050931 1102 42-43 -0.04294601935415443 1102 44-45 -0.09306885649714047 1102 46-47 0.16437811614466824 1102 48-49 0.018813671995474124 1102 50-51 0.025885727968578465 1102 52-53 -0.0193435948964904 1102 54-55 -0.033013036768394954 1102 56-57 0.04958384744453781 1102 58-59 0.031381690735472034 1102 60-61 -0.4165503112577369 1102 62-63 -0.26325516306403074 1102 64-65 -0.07390410647522572 1102 66-67 -0.14721848378871982 1102 68-69 -0.1179859794203253 1102 70-71 0.07404392269259574 1102 72-73 -0.0393805177175679 1102 74-75 -0.15183979182587848 1102 76-77 -0.04094955704346148 1102 78-79 -0.02507320002587221 1102 80-81 0.06313224070957801 1102 82-83 0.05687035711846633 1102 84-85 -0.19724199831797407 1102 86-87 -0.18773970980864263 1102 88-89 -0.4408394408664762 1102 90-91 -0.5144833856087665 1102 92-93 -0.4617645374087509 1102 94-95 -0.20080543887544522 1102 96-97 -0.5457418743789937 1102 98-99 -0.6805686010369776 1102 100-101 -0.2951930601544568 1103 1 0.21725263953999985 1103 2 0.41705782283684556 1103 3 0.4381076842163658 1103 4 0.3327211434031625 1103 5 0.32793469062495717 1103 6 0.13289298224749757 1103 7 0.2170975478171968 1103 8 -0.04204438066793159 1103 9 0.36923595506331 1103 10-11 0.3900051201792962 1103 12-13 0.45705705588829204 1103 14-15 0.5384919877156236 1103 16-17 0.6142965362300075 1103 18-19 0.4089306644686843 1103 20-21 0.2630385985894037 1103 22-23 0.20626564507536926 1103 24-25 0.16702000302127118 1103 26-27 0.3524376900806132 1103 28-29 0.5023049995881692 1103 30-31 0.1401521481612562 1103 32-33 0.2825830845282269 1103 34-35 0.2585227641590606 1103 36-37 0.46252960399659315 1103 38-39 0.3701518730749882 1103 40-41 0.3329903022549203 1103 42-43 0.4898315498595025 1103 44-45 0.6186684565239418 1103 46-47 0.5639770686787884 1103 48-49 0.6108073340163855 1103 50-51 0.14355837284124107 1103 52-53 0.4224707493850417 1103 54-55 0.5100463286373582 1103 56-57 0.44847071942361794 1103 58-59 0.5097274028231382 1103 60-61 0.5663266932685787 1103 62-63 0.6181334757849299 1103 64-65 0.6297551983521359 1103 66-67 0.5698329481224391 1103 68-69 0.44442985429339643 1103 70-71 0.45230854362968387 1103 72-73 0.4206197880184632 1103 74-75 0.2923352968020225 1103 76-77 0.35463003315648933 1103 78-79 0.6956593292927877 1103 80-81 0.6125135873986025 1103 82-83 0.48573784685497756 1103 84-85 0.302120755354057 1103 86-87 0.31865426164556254 1103 88-89 0.3520350608904437 1103 90-91 0.6010101790032145 1103 92-93 0.5468154466929747 1103 94-95 0.3610915323469719 1103 96-97 0.32193041498665664 1103 98-99 0.22507077122295271 1103 100-101 0.2918778962020774 1104 1 0.2574524123672255 1104 2 0.3433793324284089 1104 3 0.2511663220023479 1104 4 0.1832585430658824 1104 5 0.24070572388140477 1104 6 0.22195388543329386 1104 7 0.39286773629466865 1104 8 0.3662970998784232 1104 9 0.493571126661017 1104 10-11 0.5567750112439356 1104 12-13 0.3522352504658883 1104 14-15 0.3176768076384846 1104 16-17 0.16883777694772562 1104 18-19 0.4068721089458265 1104 20-21 0.36173852486898994 1104 22-23 0.4503221345282853 1104 24-25 0.45060906392446043 1104 26-27 0.2251355091586973 1104 28-29 0.4137621958711435 1104 30-31 0.318364918561727 1104 32-33 0.1894758640409151 1104 34-35 0.2841781797618381 1104 36-37 0.3124555671870084 1104 38-39 0.387064063520441 1104 40-41 0.16385930139748695 1104 42-43 0.3692081061353889 1104 44-45 0.0769875520092782 1104 46-47 0.1382270504859875 1104 48-49 -0.08901097522334567 1104 50-51 -0.16359749046276306 1104 52-53 0.16142811177623173 1104 54-55 0.49610114751611434 1104 56-57 0.5159213378208136 1104 58-59 0.44623292869013653 1104 60-61 0.40422404787642563 1104 62-63 0.2961358424316529 1104 64-65 0.25956007655525326 1104 66-67 0.29398769454636664 1104 68-69 0.5118037465097274 1104 70-71 0.3459079190627534 1104 72-73 -0.05758008921964475 1104 74-75 -0.07264481767027675 1104 76-77 -0.02966253563604937 1104 78-79 0.11758134385336305 1104 80-81 -0.043178071437250054 1104 82-83 -0.2577700899542883 1104 84-85 0.10584846125706449 1104 86-87 0.10314030697254495 1104 88-89 0.1021173240121982 1104 90-91 0.10605749611805848 1104 92-93 -0.21264520780507823 1104 94-95 -0.009558409594248474 1104 96-97 0.2006860428915722 1104 98-99 0.19076269899592901 1104 100-101 0.29827029445683095 1105 1 0.09606006279578239 1105 2 0.24925102296324653 1105 3 0.1697304292047015 1105 4 0.39492726014658786 1105 5 0.2939137373855303 1105 6 0.28947588085377873 1105 7 0.3575532384676734 1105 8 -0.01709786893159304 1105 9 0.1299551930519769 1105 10-11 0.23567809664790929 1105 12-13 0.1064889751283502 1105 14-15 0.31249716645017855 1105 16-17 0.16058482199807145 1105 18-19 0.08857417101938125 1105 20-21 -0.18960335036923937 1105 22-23 -0.2999254379691578 1105 24-25 -0.23359133459111803 1105 26-27 -0.16436686319956806 1105 28-29 -0.1707638539239298 1105 30-31 -0.20572783090968016 1105 32-33 -0.04565548089841087 1105 34-35 -0.21477130991117832 1105 36-37 -0.15545022328682734 1105 38-39 -0.24207764208563276 1105 40-41 -0.3736999614133012 1105 42-43 -0.4145796561691739 1105 44-45 -0.10148453113447431 1105 46-47 -0.19641990144203447 1105 48-49 0.02041583791640278 1105 50-51 0.10652994592287257 1105 52-53 0.18022654761642798 1105 54-55 0.10476286242518995 1105 56-57 0.29730420903420196 1105 58-59 0.16641347895173908 1105 60-61 0.1877010178659333 1105 62-63 0.09408922773752693 1105 64-65 0.09437869105372698 1105 66-67 -0.10733170766180677 1105 68-69 0.0960190039897384 1105 70-71 0.08900473029206779 1105 72-73 0.14958733070115215 1105 74-75 0.1381997344888397 1105 76-77 0.08822027420335488 1105 78-79 0.28100872551002 1105 80-81 0.3035085640778874 1105 82-83 0.3021969065321706 1105 84-85 -0.040301913308816495 1105 86-87 0.12772727852177823 1105 88-89 0.06541222092698007 1105 90-91 0.2591696646870112 1105 92-93 0.19596200885246873 1105 94-95 0.13691267864492573 1105 96-97 0.16613041360519887 1105 98-99 -0.21940655476103288 1105 100-101 -0.07417587871378473 1106 1 -0.007857322662481181 1106 2 -0.21502102238259724 1106 3 -0.14715328607154632 1106 4 -0.005011587936024853 1106 5 0.14959574316841895 1106 6 0.16638482129595644 1106 7 0.4411397347229169 1106 8 0.36226803027496857 1106 9 0.5143146346797636 1106 10-11 0.3813125557533539 1106 12-13 0.2831864318562083 1106 14-15 0.19370133900687136 1106 16-17 0.2199048360332867 1106 18-19 0.2783688608883921 1106 20-21 0.241635777515242 1106 22-23 0.15784815448834877 1106 24-25 -0.04015592922345235 1106 26-27 0.003749684286333377 1106 28-29 -0.0670872010571344 1106 30-31 -0.05518794759730383 1106 32-33 -0.030064661542340332 1106 34-35 -0.061246820213845865 1106 36-37 0.1061674466446405 1106 38-39 0.23787785290782182 1106 40-41 0.2036532328454328 1106 42-43 0.07115421726864923 1106 44-45 -0.11675233884353986 1106 46-47 0.08442426043431084 1106 48-49 0.12006904496819715 1106 50-51 0.2902359856832959 1106 52-53 0.2170477520437828 1106 54-55 0.5748002808163051 1106 56-57 0.4673378585137513 1106 58-59 0.5046369308182292 1106 60-61 0.37843873187773625 1106 62-63 0.36976812284128613 1106 64-65 0.24420778949291844 1106 66-67 0.40668784715010986 1106 68-69 0.22864939107819282 1106 70-71 0.3382383407023397 1106 72-73 0.5000589489390705 1106 74-75 0.28269529221609346 1106 76-77 0.20127780106481197 1106 78-79 0.32051806630614976 1106 80-81 0.1802179114563458 1106 82-83 0.042478024869094355 1106 84-85 0.23919048331020676 1106 86-87 0.5103968145156834 1106 88-89 0.48588048961109465 1106 90-91 0.6216468587907329 1106 92-93 0.4952003171313848 1106 94-95 0.26098498229392675 1106 96-97 0.4496701435069461 1106 98-99 0.7115188510472308 1106 100-101 1.1406189040509283 1107 1 0.4666241287619961 1107 2 0.326393737674195 1107 3 0.4133990028971901 1107 4 0.3945310745480626 1107 5 0.38306599203578884 1107 6 0.43413741110097703 1107 7 0.5227685364792691 1107 8 0.5260371660757315 1107 9 0.6717317837305927 1107 10-11 0.7521886723108295 1107 12-13 0.6419229885802977 1107 14-15 0.760164590942054 1107 16-17 0.7148009189402345 1107 18-19 0.7296651113567165 1107 20-21 0.8355534512323004 1107 22-23 0.8696570847360832 1107 24-25 0.6702103417200007 1107 26-27 0.7727337535118579 1107 28-29 0.6772648603488562 1107 30-31 0.866770198001376 1107 32-33 0.5646185824571788 1107 34-35 0.3662932486632897 1107 36-37 0.676586433064152 1107 38-39 0.7829282941389835 1107 40-41 0.5826468138064698 1107 42-43 0.6994050395222402 1107 44-45 0.8105986704649624 1107 46-47 0.6920105607701217 1107 48-49 0.7266989873033012 1107 50-51 0.7814580224942702 1107 52-53 0.6811917580605069 1107 54-55 0.6332580524434093 1107 56-57 0.8321360728513056 1107 58-59 0.9005408014586251 1107 60-61 0.8289047792677451 1107 62-63 0.8645186955500748 1107 64-65 0.6878030596165345 1107 66-67 0.8191397873091333 1107 68-69 0.7943062883998309 1107 70-71 0.7339932448453297 1107 72-73 0.6655023160934519 1107 74-75 0.7966359295853245 1107 76-77 0.8944707579564657 1107 78-79 0.9463204461755552 1107 80-81 0.8101122040002764 1107 82-83 0.799939829306151 1107 84-85 1.0808574149598122 1107 86-87 0.9093076654980301 1107 88-89 0.8117192988745288 1107 90-91 0.8297330091231601 1107 92-93 0.876564919373557 1107 94-95 0.7832438950527845 1107 96-97 1.1487858147824745 1107 98-99 1.3184304817706902 1107 100-101 1.713069503165844 1108 1 0.12802992490670206 1108 2 -0.016975023878483597 1108 3 -0.1766689928778291 1108 4 0.006291703013864947 1108 5 0.24534649111157592 1108 6 0.070049742761924 1108 7 0.2642278050295772 1108 8 -0.013596778999527714 1108 9 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-2.6409059193065687 2316 60-61 -2.6537627877920045 2316 62-63 -2.44500320214879 2316 64-65 -2.40440318714149 2316 66-67 -3.274107104006145 2316 68-69 -2.8618036441349197 2316 70-71 -2.4003386308361883 2316 72-73 -2.137339703463603 2316 74-75 -2.545068864606339 2316 76-77 -3.114553380821839 2316 78-79 -2.9898838670391825 2316 80-81 -2.918552173676609 2316 82-83 -2.700879999469276 2316 84-85 -2.8383545216186796 2316 86-87 -3.097181909588169 2316 88-89 -3.3072245271965066 2316 90-91 -3.2608363904666824 2316 92-93 -3.3788635355101633 2316 94-95 -3.4838127189018095 2316 96-97 -3.818485673001998 2316 98-99 -4.42764341362847 2316 100-101 -5.056490760271398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2720.0 3 1053.0 4 983.0 5 1156.0 6 1223.0 7 1385.0 8 1715.0 9 2212.0 10 2844.0 11 3679.0 12 4630.0 13 5728.0 14 6792.0 15 8251.0 16 10189.0 17 12968.0 18 10979.0 19 7640.0 20 7576.0 21 8018.0 22 8714.0 23 9918.0 24 11410.0 25 13158.0 26 15391.0 27 18417.0 28 21817.0 29 26320.0 30 32691.0 31 40226.0 32 51006.0 33 66399.0 34 89200.0 35 122514.0 36 174954.0 37 234036.0 38 194971.0 39 32910.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.99143497375183 18.519024788007698 14.212807716740992 22.27673252149948 2 15.527497367075846 20.164683183969697 40.1176263309797 24.190193117974754 3 19.960385307009922 24.569879559956135 29.557901922426627 25.91183321060732 4 12.667969617484093 16.296421574399783 37.4455578096833 33.59005099843282 5 13.58053672443651 37.57268653873362 33.907395946705364 14.93938079012451 6 31.62521739130435 35.80924901185771 17.553438735177863 15.01209486166008 7 28.60174045531272 33.033721716810916 20.410758201255554 17.95377962662081 8 26.742555049369592 33.80175509994429 19.60235646467081 19.853333386015308 9 26.48510137983402 17.039207893254275 20.754716981132077 35.72097374577963 10-11 25.11687335469309 26.572193132601114 26.88243265727464 21.42850085543115 12-13 26.443230724846167 24.043938932866464 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21.873234707381144 38-39 21.890379226188642 26.65952442465053 29.661991816444377 21.788104532716446 40-41 22.021692165910267 26.709560646143238 29.572157046003717 21.69659014194278 42-43 21.808550769577163 26.86866133272239 29.03856674770944 22.284221149991012 44-45 22.012295897891274 26.803264053696196 28.924946357525904 22.259493690886625 46-47 22.32992182188853 27.16494827035224 28.51381794466344 21.991311963095793 48-49 22.28450601634854 27.29287621039958 28.288214340168793 22.134403433083083 50-51 21.930781947135465 27.553430387922095 28.37987568954382 22.135911975398617 52-53 22.324845758562923 27.665180244929754 27.906294204061254 22.10367979244607 54-55 21.905491749044547 27.38250659187644 28.203316182933214 22.5086854761458 56-57 22.20754514255377 27.584359851094355 27.924588076171936 22.28350693017994 58-59 22.631134657613412 27.243388310430927 27.855980097482202 22.269496934473455 60-61 22.179512905753867 27.31274174587926 28.635352411770654 21.87239293659622 62-63 22.00549697974223 27.497381073894005 28.50928197973433 21.98783996662943 64-65 21.770926039384197 27.821433974725885 28.201639882394907 22.20600010349501 66-67 21.792184665752334 27.68836785256066 27.833181120078876 22.686266361608133 68-69 21.769429562625966 27.57023116614904 28.179571514237427 22.480767756987564 70-71 22.008506913101414 27.58236405590212 27.955050125367737 22.45407890562873 72-73 21.99927632010353 27.39539230797794 28.240462396691928 22.364868975226603 74-75 21.719729774356374 28.16543775780027 27.896744793826933 22.218087674016417 76-77 21.882578242920125 27.659176925048996 27.855423666614392 22.60282116541649 78-79 22.019816847283828 27.684945716063897 27.83241625258083 22.462821184071444 80-81 22.055803879222637 27.817821697680458 27.7104715937085 22.415902829388404 82-83 21.937190375158355 27.84833127986629 27.97011721550659 22.24436112946876 84-85 21.875869098034258 27.9013257695468 27.73335282219834 22.489452310220592 86-87 21.926975788563436 27.968754135545964 27.629259143235362 22.47501093265524 88-89 21.838369401751336 27.925934616635047 27.305141463017673 22.930554518595944 90-91 21.842525694997537 27.64922377719624 27.691930253726248 22.816320274079974 92-93 22.044150722603923 27.63894337571256 27.82024128543339 22.496664616250122 94-95 21.7856557352764 27.404375848099566 27.920810462215496 22.88915795440854 96-97 21.45275261467187 27.436120473208604 28.22178611438982 22.88934079772971 98-99 21.528386902024373 27.59696336088024 27.695858798019408 23.178790939075984 100-101 21.297309900845324 27.47671307584997 27.981180766601195 23.244796256703513 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 172.0 1 135.0 2 159.5 3 233.0 4 229.0 5 234.5 6 385.0 7 752.5 8 1227.0 9 1524.0 10 1483.0 11 1383.0 12 1430.0 13 1564.5 14 1672.5 15 1767.0 16 1864.0 17 1966.0 18 2125.0 19 2332.5 20 2545.5 21 2899.0 22 3522.5 23 4198.5 24 4988.5 25 6125.0 26 7689.0 27 9500.5 28 11234.0 29 13405.5 30 15984.0 31 18655.0 32 21762.5 33 24139.0 34 26375.5 35 28429.0 36 30624.0 37 33220.5 38 34753.5 39 35521.5 40 36428.0 41 38500.0 42 40112.5 43 41318.0 44 43123.5 45 45288.5 46 46297.0 47 48942.5 48 56900.0 49 56088.5 50 47704.0 51 43701.5 52 41021.5 53 39728.0 54 38882.0 55 36285.5 56 33033.0 57 29854.5 58 26982.0 59 23783.5 60 20078.5 61 16630.0 62 13743.5 63 10855.5 64 8047.0 65 6208.5 66 4704.5 67 3654.5 68 2638.0 69 1728.0 70 1253.5 71 930.0 72 773.5 73 668.5 74 517.5 75 411.0 76 304.5 77 187.5 78 104.0 79 72.0 80 56.5 81 36.5 82 23.0 83 17.5 84 16.0 85 12.5 86 8.0 87 7.0 88 4.5 89 2.0 90 2.0 91 1.0 92 1.0 93 2.5 94 1.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015089235844756252 2 0.007900123478929976 3 0.008769137061612274 4 0.03713058035097089 5 0.04819075322147285 6 0.0634379915358077 7 0.030573477863459007 8 0.027492429706676313 9 0.017064266714488747 10-11 0.05146930446522879 12-13 0.050402787795573244 14-15 0.02370037043678993 16-17 0.02575440254131172 18-19 0.03369402663763634 20-21 0.026149408715258223 22-23 0.01674826177533155 24-25 0.004700573469963335 26-27 0.003594556182913139 28-29 0.0023305364262843427 30-31 0.008334630270271124 32-33 0.0015010234609966954 34-35 0.002488538895862942 36-37 0.006162096313565382 38-39 0.007426116070194177 40-41 0.0036735574177024386 42-43 8.295129652876474E-4 44-45 0.0011850185218394962 46-47 0.0031205487741773406 48-49 1.5800246957859952E-4 50-51 0.001619525313180645 52-53 9.085142000769472E-4 54-55 0.00319955000896664 56-57 0.0027650432176254914 58-59 0.002646541365441542 60-61 8.690135826822975E-4 62-63 0.001343020991418096 64-65 0.0032785512437559397 66-67 0.0027650432176254914 68-69 0.007110111131036979 70-71 0.00999365620084642 72-73 0.003950061739464988 74-75 0.003634056800307789 76-77 0.003910561122070337 78-79 0.036775074794419035 80-81 0.025082892045602677 82-83 0.004542571000384736 84-85 0.00971715187908387 86-87 0.007860622861535327 88-89 0.006596603104906529 90-91 0.014773230905599054 92-93 0.0163137549839904 94-95 0.008334630270271124 96-97 0.0048190753221472845 98-99 0.02567540130652242 100-101 0.048032750751894254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1265803.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.40650214356569 #Duplication Level Percentage of deduplicated Percentage of total 1 82.6899450077211 52.430801753833975 2 10.566504331019326 13.399701590295463 3 2.963894963963222 5.637906371575128 4 1.2007314557756028 3.045367264979301 5 0.6334960310042586 2.0083883723905944 6 0.3957971207476121 1.5057666591060355 7 0.27722693345152793 1.2304593105103911 8 0.19819331659869932 1.0053395963004659 9 0.1431498113914341 0.8168965940557793 >10 0.8369931736723926 9.83240043385615 >50 0.05908454795366543 2.5793636283957024 >100 0.03198475530869511 3.7178019626387377 >500 0.0017491549789689091 0.7677196281191931 >1k 0.0011244567721942988 1.5975476236355022 >5k 1.249396413549221E-4 0.42453921030757985 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5359 0.4233676172358574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4267 0.3370982688459421 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4061 0.3208240144793463 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2649 0.20927427095685505 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2548 0.20129514624313577 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1614 0.1275079929499298 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1604 0.12671798060203682 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.950061739464988E-5 0.0 12-13 3.950061739464988E-5 0.0 0.0 7.900123478929976E-5 0.0 14-15 1.975030869732494E-4 0.0 0.0 7.900123478929976E-5 0.0 16-17 2.3700370436789927E-4 0.0 0.0 7.900123478929976E-5 0.0 18-19 2.3700370436789927E-4 0.0 0.0 7.900123478929976E-5 0.0 20-21 2.3700370436789927E-4 0.0 0.0 1.1850185218394965E-4 0.0 22-23 2.3700370436789927E-4 0.0 0.0 2.3700370436789927E-4 0.0 24-25 3.555055565518489E-4 0.0 0.0 3.1600493915719905E-4 0.0 26-27 3.950061739464988E-4 0.0 0.0 6.320098783143981E-4 0.0 28-29 3.950061739464988E-4 0.0 0.0 0.0011060172870501967 0.0 30-31 3.950061739464988E-4 0.0 0.0 0.0025280395132575924 0.0 32-33 4.7400740873579855E-4 0.0 0.0 0.005767090139618882 0.0 34-35 5.530086435250984E-4 0.0 0.0 0.009243144470348071 0.0 36-37 6.320098783143981E-4 0.0 0.0 0.01248219509670936 0.0 38-39 6.320098783143981E-4 0.0 0.0 0.01635325560138505 0.0 40-41 6.320098783143981E-4 0.0 0.0 0.02283135685410763 0.0 42-43 6.320098783143981E-4 0.0 0.0 0.02962546304598741 0.0 44-45 6.320098783143981E-4 0.0 0.0 0.036696073559629734 0.0 46-47 6.320098783143981E-4 0.0 0.0 0.04676873099526546 3.950061739464988E-5 48-49 6.320098783143981E-4 0.0 0.0 0.054668854474195436 7.900123478929976E-5 50-51 6.320098783143981E-4 0.0 0.0 0.06545252302293485 7.900123478929976E-5 52-53 6.320098783143981E-4 0.0 0.0 0.08986390457282847 7.900123478929976E-5 54-55 6.320098783143981E-4 0.0 0.0 0.12454544664533107 7.900123478929976E-5 56-57 6.320098783143981E-4 0.0 0.0 0.158397475752546 7.900123478929976E-5 58-59 6.320098783143981E-4 0.0 0.0 0.216502883940076 7.900123478929976E-5 60-61 7.110111131036978E-4 0.0 0.0 0.2816394020238536 7.900123478929976E-5 62-63 7.110111131036978E-4 0.0 0.0 0.32501107992317924 7.900123478929976E-5 64-65 7.110111131036978E-4 0.0 0.0 0.3626156676828859 7.900123478929976E-5 66-67 7.110111131036978E-4 0.0 0.0 0.46271023216092866 7.900123478929976E-5 68-69 7.110111131036978E-4 0.0 0.0 0.5666363565262524 7.900123478929976E-5 70-71 7.110111131036978E-4 0.0 0.0 0.6132075844345447 7.900123478929976E-5 72-73 7.110111131036978E-4 0.0 0.0 0.7048095161727378 7.900123478929976E-5 74-75 7.110111131036978E-4 0.0 0.0 0.8327520159140087 7.900123478929976E-5 76-77 7.110111131036978E-4 0.0 0.0 0.9715176848214138 7.900123478929976E-5 78-79 7.110111131036978E-4 0.0 0.0 1.1048322685283571 7.900123478929976E-5 80-81 7.900123478929976E-4 0.0 0.0 1.2543026047497121 7.900123478929976E-5 82-83 7.900123478929976E-4 0.0 0.0 1.3929497718049333 7.900123478929976E-5 84-85 7.900123478929976E-4 0.0 0.0 1.5470416802614624 7.900123478929976E-5 86-87 7.900123478929976E-4 0.0 0.0 1.8052967167876834 7.900123478929976E-5 88-89 7.900123478929976E-4 0.0 0.0 2.1886897092201547 7.900123478929976E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6330 0.0 26.128902 1 TACATGG 6390 0.0 24.98905 2 ACATGGG 6305 0.0 24.421438 3 GAGTACT 5125 0.0 22.991892 12-13 GTATCAA 11030 0.0 22.966635 1 GGACCGT 105 0.0021889475 22.63218 6 GTACTTT 5495 0.0 21.225912 14-15 AGTACTT 5295 0.0 21.087194 12-13 CATGGGA 3905 0.0 20.446396 4 CATGGGG 2445 0.0 20.409807 4 TCAACGC 12470 0.0 19.9707 4 ATCAACG 12530 0.0 19.874287 3 CAACGCA 12615 0.0 19.704258 5 AGAGTAC 9090 0.0 19.682402 10-11 GTACCTG 1140 0.0 19.5947 1 TATCAAC 12840 0.0 19.46848 2 AACGCAG 12810 0.0 19.441412 6 ATGGGAG 1735 0.0 19.175392 5 GGTATCA 8450 0.0 19.011028 1 TACCTGG 1055 0.0 18.468971 2 >>END_MODULE