FastQCFastQC Report
Thu 26 May 2016
SRR936666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265803
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73380.5797110608838817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65840.5201441298527496No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49830.3936631529550807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.3025747292430181No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23290.18399387582427915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.15428941154350242No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15700.12403193861920063No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14340.11328777068785585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG702.0547786E-433.9248355
TACCTGG10500.026.695972
ACCGTGC2059.586074E-1025.4849958
GTACCTG12050.025.100021
GTACATG63600.023.023071
GAGTACT75800.022.24007812-13
GTACACG2605.475158E-1022.1578921
GTGTAGG6100.022.0368121
TACATGG65450.021.8494192
GTATCAA132550.021.7316171
ACATGGG64800.021.4811953
GTACTTT78650.021.37379614-15
AGTACTT77050.020.55418612-13
ATGGGCG1404.8557168E-420.35495
ATGGGAG16800.020.35495
GTACGTG1707.3292504E-519.7683141
TATTTCG1456.151073E-419.6530065
GGTATCA103750.019.2035061
AGAGTAC116800.019.08841510-11
ACTTTTT90850.018.8433216-17
Produced by FastQC (version 0.11.5)