##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936666_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1265803 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.565083192250295 31.0 31.0 34.0 30.0 34.0 2 31.78409831545667 31.0 31.0 34.0 30.0 34.0 3 32.04465228791526 33.0 31.0 34.0 30.0 34.0 4 35.84020657242873 37.0 35.0 37.0 35.0 37.0 5 35.66815136320581 37.0 35.0 37.0 33.0 37.0 6 35.78299387819432 37.0 35.0 37.0 35.0 37.0 7 35.72495404103166 37.0 35.0 37.0 33.0 37.0 8 35.702966417365104 37.0 35.0 37.0 33.0 37.0 9 37.33985778197713 39.0 37.0 39.0 34.0 39.0 10-11 37.23550584095629 39.0 37.0 39.0 33.5 39.0 12-13 37.110119426166634 39.0 37.0 39.0 33.0 39.0 14-15 38.224520324252666 40.0 37.5 41.0 33.0 41.0 16-17 38.14865464847216 40.0 37.0 41.0 33.0 41.0 18-19 38.23549280575256 40.0 37.5 41.0 33.5 41.0 20-21 38.31364675229874 40.0 38.0 41.0 34.0 41.0 22-23 38.23546871037594 40.0 38.0 41.0 33.5 41.0 24-25 38.12558865795072 40.0 37.0 41.0 33.0 41.0 26-27 37.92146961257005 40.0 37.0 41.0 33.0 41.0 28-29 37.834478982906504 40.0 37.0 41.0 33.0 41.0 30-31 37.7975640759265 40.0 37.0 41.0 33.0 41.0 32-33 37.58734060513366 40.0 36.5 41.0 33.0 41.0 34-35 37.3981460780232 39.5 36.0 41.0 32.0 41.0 36-37 37.147734679093034 39.0 36.0 41.0 31.5 41.0 38-39 36.97244515931784 39.0 35.5 41.0 31.0 41.0 40-41 36.73000103491618 39.0 35.0 41.0 31.0 41.0 42-43 36.45702451329314 39.0 35.0 40.5 30.0 41.0 44-45 36.12686847795431 39.0 35.0 40.0 30.0 41.0 46-47 35.653862804875644 39.0 35.0 40.0 28.5 41.0 48-49 35.12351566554985 38.0 35.0 40.0 26.0 41.0 50-51 34.9002988616712 38.0 34.0 40.0 26.0 41.0 52-53 34.62951659934445 38.0 34.0 40.0 24.5 41.0 54-55 34.35352736563272 38.0 34.0 40.0 24.0 41.0 56-57 34.07242833205483 38.0 33.0 40.0 23.0 41.0 58-59 33.688992283949396 37.0 33.0 40.0 22.5 41.0 60-61 33.311680411564836 37.0 33.0 40.0 22.0 41.0 62-63 33.02919253627934 36.0 32.0 39.0 21.0 40.5 64-65 32.60137082942606 36.0 32.0 39.0 19.5 40.0 66-67 32.197369969892634 35.0 31.0 39.0 18.0 40.0 68-69 31.746880043735082 35.0 31.0 38.0 17.0 40.0 70-71 31.27247762882534 35.0 30.5 37.5 13.5 39.5 72-73 30.864254943304765 34.5 30.0 37.0 9.5 39.0 74-75 30.48611711301048 34.0 30.0 36.5 7.5 39.0 76-77 29.095255343841025 32.5 28.0 35.0 7.0 37.5 78-79 29.87865844843155 34.0 29.5 36.0 7.0 37.5 80-81 29.715085206781783 34.0 29.0 35.0 2.0 37.0 82-83 29.373103081601165 34.0 29.0 35.0 2.0 37.0 84-85 28.925365953469854 34.0 29.0 35.0 2.0 36.0 86-87 28.546718960217348 34.0 29.0 35.0 2.0 36.0 88-89 28.126339169681223 33.0 27.5 35.0 2.0 35.5 90-91 27.67078131431194 33.0 27.0 35.0 2.0 35.0 92-93 27.2213314394104 33.0 26.0 35.0 2.0 35.0 94-95 26.69847401214881 32.5 25.0 34.5 2.0 35.0 96-97 26.017550124308443 32.0 24.5 34.0 2.0 35.0 98-99 25.156691444087272 32.0 21.0 34.0 2.0 35.0 100-101 22.38113276710515 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2572076496513134 1101 2 0.30160817812581087 1101 3 0.22193390111107192 1101 4 0.09249678338812828 1101 5 0.16693170284437286 1101 6 0.16932567851002744 1101 7 0.08654583827291873 1101 8 0.06265504213509843 1101 9 0.16960316455942603 1101 10-11 0.12033944321207457 1101 12-13 0.12667326861974004 1101 14-15 0.38524345643241276 1101 16-17 0.16721068167044706 1101 18-19 0.08825844586876741 1101 20-21 0.01797668770097971 1101 22-23 -0.06674673613780868 1101 24-25 -0.07310853075912149 1101 26-27 -0.11586420940464137 1101 28-29 -0.04920293940244136 1101 30-31 -0.20428264024149456 1101 32-33 0.014440130076465607 1101 34-35 0.24751435857150028 1101 36-37 0.21515256758125645 1101 38-39 0.16776724246488328 1101 40-41 0.19794279684210636 1101 42-43 0.15287573678664046 1101 44-45 0.3761581593024417 1101 46-47 0.5935561299196479 1101 48-49 0.6646746823316363 1101 50-51 0.5366686732889718 1101 52-53 0.4351277523361219 1101 54-55 0.29608609390858476 1101 56-57 0.2688009492630101 1101 58-59 0.35319692706401185 1101 60-61 0.4775264295914212 1101 62-63 0.3104519955951801 1101 64-65 0.49211813922148906 1101 66-67 0.41795728222608375 1101 68-69 0.17793756890153034 1101 70-71 0.048803076236865195 1101 72-73 0.3290883738864494 1101 74-75 0.33642124278348007 1101 76-77 0.694749256095804 1101 78-79 0.5627776692600293 1101 80-81 0.3910202415400086 1101 82-83 0.5556543175934117 1101 84-85 0.586637204483516 1101 86-87 0.5890189343335912 1101 88-89 0.4088170680208094 1101 90-91 0.4563294423091122 1101 92-93 0.4848770307952286 1101 94-95 0.3132174790821054 1101 96-97 0.15392952538463334 1101 98-99 0.04031185504358703 1101 100-101 -0.1833972549217684 1102 1 0.44305918505596864 1102 2 0.5258410619267337 1102 3 0.16897336763394577 1102 4 0.06717135664494123 1102 5 0.13309335029999403 1102 6 0.11551006773933636 1102 7 0.05460118217106924 1102 8 -0.12630665689979992 1102 9 -0.028539981341282328 1102 10-11 0.10848458194861621 1102 12-13 0.012096428051627583 1102 14-15 0.14279789518117525 1102 16-17 0.25410736736674266 1102 18-19 0.22863648180344143 1102 20-21 0.19820390447964797 1102 22-23 0.009570503231913108 1102 24-25 0.12309905239991537 1102 26-27 -0.11822379402396876 1102 28-29 -0.03992285553587038 1102 30-31 0.08917583425104425 1102 32-33 0.15226700120697245 1102 34-35 0.28693114427800737 1102 36-37 0.34974116420616497 1102 38-39 0.10858973103119496 1102 40-41 0.2743159695874837 1102 42-43 0.13902937940585502 1102 44-45 0.21738206194487475 1102 46-47 0.33474252035806273 1102 48-49 0.3877413385038153 1102 50-51 0.33840562230754045 1102 52-53 0.19176732871616053 1102 54-55 0.2971072966771118 1102 56-57 0.3164747739503966 1102 58-59 0.4013250848296295 1102 60-61 0.41040621135077515 1102 62-63 0.3615983785981385 1102 64-65 0.26207689254357547 1102 66-67 0.33026664403297445 1102 68-69 0.381958743200979 1102 70-71 0.23363357633818183 1102 72-73 0.1578021239513383 1102 74-75 -0.19200731405169336 1102 76-77 0.17459582448272926 1102 78-79 0.3494117695281389 1102 80-81 0.306117061543393 1102 82-83 0.3668637679893827 1102 84-85 0.39510876638116343 1102 86-87 0.21711691487078255 1102 88-89 0.3295418908844958 1102 90-91 0.2884244821574562 1102 92-93 0.43184783658751513 1102 94-95 0.3180408116730078 1102 96-97 0.2585698185157135 1102 98-99 -0.17590128795996662 1102 100-101 -0.23478696485530293 1103 1 0.43434357502725973 1103 2 0.43601688147705886 1103 3 0.32469326223322525 1103 4 0.1351382546755744 1103 5 0.06626905182199039 1103 6 0.04575816897635576 1103 7 0.16516706402044434 1103 8 0.2514223927161794 1103 9 0.10966479354456737 1103 10-11 0.11537217489592422 1103 12-13 0.13286980512654623 1103 14-15 0.22038801265491514 1103 16-17 0.21209230382341815 1103 18-19 0.14226711854495733 1103 20-21 0.17753632629299432 1103 22-23 0.16719591544229928 1103 24-25 -0.01768723668986638 1103 26-27 -0.006603478362173121 1103 28-29 0.12773638672225474 1103 30-31 0.14472896050865103 1103 32-33 0.14394849251625175 1103 34-35 0.12783055570059076 1103 36-37 -0.017173717459776583 1103 38-39 0.06871677501879248 1103 40-41 0.15940916930705384 1103 42-43 0.2166584248979575 1103 44-45 0.3323937367953178 1103 46-47 0.24187911042007215 1103 48-49 0.32177665599291316 1103 50-51 0.05214319555975777 1103 52-53 0.20875452050935195 1103 54-55 0.3040915875330512 1103 56-57 0.22983682514055914 1103 58-59 0.19447492677121403 1103 60-61 0.21003659070520797 1103 62-63 0.036893200567327256 1103 64-65 0.04454303761035305 1103 66-67 0.038602643668930625 1103 68-69 -0.031073752984429603 1103 70-71 -4.626395029276864E-4 1103 72-73 0.053350429533796984 1103 74-75 0.0498217126696332 1103 76-77 -0.14931355109052902 1103 78-79 -0.1538116125949749 1103 80-81 -0.14020208990401883 1103 82-83 0.16612584207420156 1103 84-85 0.25876542623631593 1103 86-87 0.24320241999648218 1103 88-89 0.15037062040196147 1103 90-91 -0.050304736679866124 1103 92-93 0.13796440591636028 1103 94-95 0.08197597572527471 1103 96-97 -0.2581573198945435 1103 98-99 -0.18533057832530275 1103 100-101 -0.19124588744151438 1104 1 0.34384306721068114 1104 2 0.35754656422616904 1104 3 0.12762879009352446 1104 4 -0.07413833608754317 1104 5 -0.13540452811086823 1104 6 -0.02036397910358545 1104 7 -0.02764108255978215 1104 8 -0.14521399479374253 1104 9 -0.030479926270402302 1104 10-11 -0.03132274598910101 1104 12-13 -0.09060627054287096 1104 14-15 0.07783235057916471 1104 16-17 -0.043551816419956424 1104 18-19 -0.00346358363407262 1104 20-21 -0.1357402296664887 1104 22-23 -0.10486325705397093 1104 24-25 -0.1148316780758094 1104 26-27 0.024621704277784318 1104 28-29 -0.20979552978700866 1104 30-31 0.02347193254868074 1104 32-33 -0.017685094883567842 1104 34-35 0.14375123823138125 1104 36-37 0.0877465664112691 1104 38-39 0.021945444349519505 1104 40-41 0.1678162230533502 1104 42-43 0.11552659508137708 1104 44-45 0.18295763467514092 1104 46-47 0.25839343211318067 1104 48-49 0.24606531728716874 1104 50-51 0.2608289156310164 1104 52-53 0.18195968157105114 1104 54-55 0.0371303556111684 1104 56-57 0.1873443632900873 1104 58-59 0.288844569780899 1104 60-61 0.004262115053506932 1104 62-63 -0.20459098434751155 1104 64-65 -0.12203543349789214 1104 66-67 0.1360306555274775 1104 68-69 0.3164598562467589 1104 70-71 0.10221874277063847 1104 72-73 0.13947714954493406 1104 74-75 0.06424307070721724 1104 76-77 0.3694436271578354 1104 78-79 0.3801915323874496 1104 80-81 0.39781375523881835 1104 82-83 0.4446952705669851 1104 84-85 0.4751198094369222 1104 86-87 0.3641826009120699 1104 88-89 0.36951957676770064 1104 90-91 0.3138970421019458 1104 92-93 0.4754354160758574 1104 94-95 0.3579339455979884 1104 96-97 0.36557155667596675 1104 98-99 0.5105707407322164 1104 100-101 0.5765458856135162 1105 1 0.37634312950758186 1105 2 0.485814498119467 1105 3 0.2098519311309417 1105 4 0.07977423228907554 1105 5 -0.06668274053130574 1105 6 -0.01692195998259649 1105 7 0.09529515956197088 1105 8 -0.06157417609156823 1105 9 -0.007195190672291574 1105 10-11 0.02374794484200038 1105 12-13 0.06871043859336368 1105 14-15 0.15320606276379323 1105 16-17 0.15321571277421953 1105 18-19 0.23182650303706964 1105 20-21 0.1631339961214664 1105 22-23 0.13073323807625314 1105 24-25 0.017401107444733555 1105 26-27 0.004485730909472352 1105 28-29 0.03227084456106866 1105 30-31 0.016714795400439186 1105 32-33 -0.10479092854992444 1105 34-35 -0.2389476203971057 1105 36-37 -0.12753006412934553 1105 38-39 -0.15824441814507395 1105 40-41 -0.49527660117433214 1105 42-43 -0.33164009573495434 1105 44-45 -0.43148061802139637 1105 46-47 -0.4174218035859951 1105 48-49 -0.3940417964651317 1105 50-51 -0.5043415333035242 1105 52-53 -0.3805933284767491 1105 54-55 -0.3042335865736234 1105 56-57 -0.3716304973725144 1105 58-59 -0.16770472644871148 1105 60-61 -0.2633023806969135 1105 62-63 -0.4017166053553183 1105 64-65 -0.42665597154007173 1105 66-67 -0.3138090337817303 1105 68-69 -0.5233533545724143 1105 70-71 -0.44407223820712005 1105 72-73 -0.4825590177282457 1105 74-75 -0.48997761352502067 1105 76-77 -0.556912899388486 1105 78-79 -0.40184476409411474 1105 80-81 -0.6602781045405486 1105 82-83 -0.47308804881491895 1105 84-85 -0.41419636440742735 1105 86-87 -0.5457863798460316 1105 88-89 -0.4611362203972824 1105 90-91 -0.5461940405812697 1105 92-93 -0.23111411088300926 1105 94-95 -0.07013920665286477 1105 96-97 -0.2402948732317114 1105 98-99 -0.20734127943167735 1105 100-101 -0.21551721237904786 1106 1 0.3117354917817323 1106 2 0.3389676173184597 1106 3 0.14236346479660966 1106 4 0.003505263649067558 1106 5 -0.12085367818873038 1106 6 -0.08567964710241682 1106 7 0.05147651958698418 1106 8 0.07405249569939798 1106 9 0.0362635447888664 1106 10-11 -0.03890065511351537 1106 12-13 0.04403176484680671 1106 14-15 0.11868230189963924 1106 16-17 0.1512516320940307 1106 18-19 0.04019699022993706 1106 20-21 -0.14213908275547738 1106 22-23 -0.10081886871876833 1106 24-25 -0.09140763515612349 1106 26-27 -0.10198761359611552 1106 28-29 -0.05478358117624538 1106 30-31 -0.03353514713693073 1106 32-33 0.034156897504722394 1106 34-35 -0.20364088142395786 1106 36-37 0.026575952699111838 1106 38-39 0.09594026807710776 1106 40-41 0.05777374883015085 1106 42-43 0.10067423361377337 1106 44-45 0.22807227228258142 1106 46-47 0.4245501228002624 1106 48-49 0.16467811129739118 1106 50-51 0.24554155081273876 1106 52-53 0.2940086664983923 1106 54-55 0.20219479640937266 1106 56-57 0.28289142681078516 1106 58-59 0.09528169538380382 1106 60-61 0.1700377826827406 1106 62-63 -0.1999163396205077 1106 64-65 0.13182877737344256 1106 66-67 0.06271107176903001 1106 68-69 -0.03076643205858076 1106 70-71 0.1717947202759298 1106 72-73 0.45822129560101743 1106 74-75 0.35160813213289543 1106 76-77 0.11562415670937298 1106 78-79 0.18033290239755573 1106 80-81 0.04305308245957562 1106 82-83 0.27424252666602555 1106 84-85 0.24036802638517685 1106 86-87 0.29590579775145187 1106 88-89 0.32042668884148995 1106 90-91 0.18750024123670173 1106 92-93 0.4839081336577742 1106 94-95 0.5047153584810928 1106 96-97 0.42765764697576003 1106 98-99 0.3478748676471781 1106 100-101 0.1138102810877264 1107 1 0.6276730406019553 1107 2 0.6037505548133595 1107 3 0.4298805561963732 1107 4 0.21174798433723652 1107 5 0.016747124778710543 1107 6 0.23306787300287368 1107 7 0.17128014648915268 1107 8 0.2506286027174838 1107 9 0.25421777509176025 1107 10-11 0.41652103413200336 1107 12-13 0.3530605171089505 1107 14-15 0.6252389015647921 1107 16-17 0.507566014308189 1107 18-19 0.5440282979414945 1107 20-21 0.551193854255537 1107 22-23 0.48203933867853976 1107 24-25 0.36854387987808224 1107 26-27 0.47403749019655095 1107 28-29 0.4987410145404354 1107 30-31 0.5608238959935363 1107 32-33 0.5186218692944067 1107 34-35 0.3740128305723047 1107 36-37 0.5194328190523123 1107 38-39 0.7251350056541312 1107 40-41 0.7188787691739336 1107 42-43 0.3645422886988854 1107 44-45 0.5369852385200673 1107 46-47 0.6467569836925122 1107 48-49 0.6203192755448868 1107 50-51 0.69198515714303 1107 52-53 0.5422452626242986 1107 54-55 0.5128808441588362 1107 56-57 0.7229260174536165 1107 58-59 0.34651663110354747 1107 60-61 0.3345666130572482 1107 62-63 0.38182306371163577 1107 64-65 0.36530559320938494 1107 66-67 0.6092656944310164 1107 68-69 0.3013797776945424 1107 70-71 0.35982638550708046 1107 72-73 0.5322953184706591 1107 74-75 0.6243097968248676 1107 76-77 0.6056465120528784 1107 78-79 0.7493797380173461 1107 80-81 0.7701772202857278 1107 82-83 0.9697926796418308 1107 84-85 0.7617753070104065 1107 86-87 0.8877019949927707 1107 88-89 0.7829144362606719 1107 90-91 0.7931454839172964 1107 92-93 0.9633497201267751 1107 94-95 0.8011362223623486 1107 96-97 0.9365275477246691 1107 98-99 0.8595416057440275 1107 100-101 1.2781628402008174 1108 1 0.3472020213254865 1108 2 0.3303382231524168 1108 3 0.04938478117656331 1108 4 0.05031248130053001 1108 5 0.05461846092870104 1108 6 -0.05084419459680589 1108 7 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-1.496069487388148 2316 54-55 -1.1409281879293118 2316 56-57 -1.0036084916367614 2316 58-59 -1.2453290837052364 2316 60-61 -1.1469065413644444 2316 62-63 -0.8729228696646274 2316 64-65 -1.0882656805042288 2316 66-67 -1.2310174476089415 2316 68-69 -1.401950606075257 2316 70-71 -1.0430218971885097 2316 72-73 -0.988539252769332 2316 74-75 -1.295175549839854 2316 76-77 -1.129869177109157 2316 78-79 -1.0713005833619604 2316 80-81 -1.186507957434845 2316 82-83 -1.2116644957469234 2316 84-85 -1.2678336804328048 2316 86-87 -1.153574898362038 2316 88-89 -1.0746003664263775 2316 90-91 -1.2457076551195563 2316 92-93 -1.3760718388808222 2316 94-95 -1.2250071617559364 2316 96-97 -1.1244348718381616 2316 98-99 -1.356600509687219 2316 100-101 -1.8518213734781988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 17.0 5 55.0 6 90.0 7 226.0 8 542.0 9 1074.0 10 1769.0 11 2793.0 12 4102.0 13 5839.0 14 7410.0 15 10167.0 16 13214.0 17 17003.0 18 14528.0 19 9075.0 20 7842.0 21 7828.0 22 8293.0 23 9037.0 24 10484.0 25 12843.0 26 15458.0 27 18915.0 28 23779.0 29 29661.0 30 36404.0 31 45589.0 32 57181.0 33 72592.0 34 96090.0 35 130856.0 36 186253.0 37 244537.0 38 154450.0 39 9797.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98398817155713 19.36276818774779 15.034640667936586 21.618602972758495 2 15.662048972890375 21.118028126503688 39.488116279602636 23.731806621003305 3 20.296806376659994 24.941516471379217 30.09454540857885 24.66713174338194 4 13.174697362484721 17.169804910308994 36.92236945757149 32.7331282696348 5 13.342755547269203 37.88472613826954 33.83788788618766 14.934630428273593 6 31.28282995063213 36.67632325093241 16.96812221175017 15.07272458668529 7 28.344142018939756 33.500868618576504 20.268319793838376 17.88666956864536 8 26.920065760627836 34.11360219560232 19.201408118008885 19.76492392576096 9 26.39998799124309 17.045482376754208 20.589882780311644 35.964646851691064 10-11 25.320044762223404 26.913346071000976 26.452293589940584 21.314315576835035 12-13 26.852967746497175 24.26409216934264 27.46815157680851 21.414788507351673 14-15 23.068123554771162 25.540704201206665 26.87633857717196 24.514833666850215 16-17 21.58250533455838 28.99179414174243 27.45454861459485 21.97115190910434 18-19 21.402619522943144 26.913745661844697 30.704619913209243 20.97901490200292 20-21 22.256227864841527 25.529841531423138 31.126012499575367 21.087918104159968 22-23 21.92176213736815 25.62370583852973 31.05414759741219 21.400384426689925 24-25 22.062477336520768 25.70478976586404 31.279511898770977 20.953220998844213 26-27 21.6856809471932 25.90766493680296 31.34077735635008 21.065876759653754 28-29 21.180744555037396 26.534421233003876 31.197271613355316 21.087562598603416 30-31 21.430072452032427 25.87468192127843 31.181155361458302 21.514090265230845 32-33 21.36124020596362 26.168519641400508 31.154703803848683 21.31553634878719 34-35 22.16169797249469 25.944388967539687 30.776501984528267 21.117411075437353 36-37 22.002833856007406 25.732984717165447 30.94662404163801 21.317557385189133 38-39 21.620846554534815 26.109109524676654 30.987086047851896 21.282957872936635 40-41 21.853289875722346 26.211955003912685 30.68944254197793 21.24531257838704 42-43 21.62690997639961 26.467834492283455 30.200599372499816 21.70465615881712 44-45 21.90510613000259 26.290970210126936 30.047658266724337 21.756265393146137 46-47 22.411806461067727 26.802256155584516 29.37671312149846 21.4092242618493 48-49 22.598965359901868 26.98079851776646 28.844651890420593 21.575584231911083 50-51 22.12090712736155 27.260629263120972 29.046854237405967 21.571609372111507 52-53 22.506192819371133 27.47964595757321 28.485705064977694 21.52845615807796 54-55 22.091624054257345 27.125076639129254 28.713955906427575 22.069343400185833 56-57 22.346823978523677 27.339808463807685 28.41868086202347 21.894686695645166 58-59 23.00027018571713 27.006602959718627 28.221609169250023 21.77151768531422 60-61 22.342990684478686 27.120483955197646 29.088862852235497 21.44766250808817 62-63 22.217631234865873 27.159504331372723 29.04796703969536 21.57489739406604 64-65 22.040583796033278 27.501918094405802 28.708678004296846 21.748820105264073 66-67 22.075658175405994 27.436339245930547 28.370433221780857 22.117569356882598 68-69 22.062218261623855 27.343573481476568 28.578126752841282 22.016081504058295 70-71 22.30224210605733 27.407249111527797 28.279901232149086 22.01060755026578 72-73 22.413795827628785 27.105205154356565 28.553021283722664 21.927977734291986 74-75 22.06180582602506 28.058039047150306 28.089363036744263 21.79079209008037 76-77 22.296918568342416 27.4428292495249 28.09222529934827 22.16802688278441 78-79 22.335342020702097 27.465672304528276 28.14302159569366 22.055964079075967 80-81 22.371874797526544 27.632510481211252 27.931539082710305 22.064075638551895 82-83 22.298392767691645 27.648433142075163 28.127078964709156 21.926095125524036 84-85 22.185545442227554 27.767781204484592 27.916630290551993 22.13004306273586 86-87 22.075055187637968 27.76258175434272 27.818717497760098 22.343645560259212 88-89 22.114896784767673 27.9209412887798 27.407907609683473 22.556254316769056 90-91 22.132918731936094 27.542439681620895 27.748805288143362 22.57583629829965 92-93 22.32467580177069 27.459034039579656 27.931431812804234 22.284858345845414 94-95 22.078306339887895 27.280965561975762 27.968051095493156 22.672677002643184 96-97 21.936495947247455 27.213409676830615 28.280659533232257 22.569434842689677 98-99 21.878383807578306 27.524525383025782 27.6225360547722 22.974554754623703 100-101 21.40702127953763 27.798270062280128 27.66395199314188 23.130756665040366 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 324.0 1 261.5 2 267.5 3 356.5 4 313.0 5 286.5 6 476.0 7 986.5 8 1559.0 9 1910.0 10 1916.0 11 1769.5 12 1818.0 13 1938.0 14 2123.5 15 2361.0 16 2574.5 17 2691.0 18 2816.5 19 3074.0 20 3336.0 21 3703.0 22 4224.0 23 4823.5 24 5683.5 25 6790.5 26 8305.0 27 10159.0 28 11853.0 29 14028.0 30 16693.5 31 19081.0 32 21751.0 33 24336.5 34 26812.5 35 28536.0 36 30410.0 37 32890.5 38 34353.5 39 35221.5 40 36195.0 41 38105.5 42 39581.5 43 40675.0 44 42089.0 45 43627.5 46 44641.5 47 48071.5 48 56314.0 49 55377.0 50 47053.5 51 42847.5 52 40101.5 53 38764.0 54 37867.5 55 35368.5 56 32063.0 57 29187.5 58 26702.0 59 23770.5 60 20236.5 61 16833.5 62 13798.0 63 10843.5 64 8112.0 65 6207.5 66 4689.5 67 3686.5 68 2706.0 69 1735.0 70 1262.5 71 1004.5 72 862.5 73 732.5 74 543.5 75 453.5 76 369.5 77 222.0 78 135.0 79 94.0 80 71.0 81 54.0 82 36.5 83 22.5 84 12.5 85 6.5 86 5.0 87 3.5 88 1.0 89 2.0 90 3.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0451073350276463 2 0.01295620250544516 3 0.004898076556936585 4 0.014220222262073957 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004740074087357985 10-11 2.765043217625492E-4 12-13 1.975030869732494E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001738027165364595 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.003594556182913139 34-35 8.690135826822975E-4 36-37 0.002883545069809441 38-39 1.1850185218394962E-4 40-41 0.003831559887281038 42-43 0.011534180279237765 44-45 0.001619525313180645 46-47 0.0016985265479699447 48-49 0.011494679661843114 50-51 3.950061739464988E-4 52-53 0.0017775277827592447 54-55 0.010349161757398268 56-57 0.002923045687204091 58-59 5.530086435250984E-4 60-61 0.005214081496093784 62-63 0.003002046921993391 64-65 0.017696276592803147 66-67 0.0024490382784682924 68-69 7.900123478929976E-5 70-71 5.135080261304485E-4 72-73 0.0 74-75 0.0 76-77 9.085142000769472E-4 78-79 0.010586165461766169 80-81 0.01619525313180645 82-83 0.011771183983605663 84-85 0.007228612983220928 86-87 0.00955914940950527 88-89 0.030731480333037606 90-91 0.02610990809786357 92-93 0.0020935327219164437 94-95 0.007465616687588827 96-97 0.00948014817471597 98-99 0.00983565373126782 100-101 0.011889685835789613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1265803.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.162719842566325 #Duplication Level Percentage of deduplicated Percentage of total 1 81.7585574347206 50.00575743112184 2 11.043850210393447 13.509438328031232 3 3.1394036202161737 5.76043392288061 4 1.2788346142613691 3.1286801294817796 5 0.6789641540752557 2.076364716942495 6 0.43790399189953344 1.6070039504695557 7 0.2946183815734357 1.2613763072852444 8 0.2007131045827066 0.9820927507459041 9 0.16238350983734387 0.8938635407310668 >10 0.9018490002692359 10.219806328400745 >50 0.061820072714723645 2.606029525409789 >100 0.037212149145685894 4.253092510534186 >500 0.002204195242663398 0.8421945871992348 >1k 0.0014262439805469047 1.7498138951554725 >5k 2.5931708737216453E-4 1.1040520756108991 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7338 0.5797110608838817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6584 0.5201441298527496 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4983 0.3936631529550807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3830 0.3025747292430181 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2329 0.18399387582427915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1953 0.15428941154350242 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1570 0.12403193861920063 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1434 0.11328777068785585 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 2 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 3 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 4 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 5 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 6 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 7 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 8 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 9 0.0 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 10-11 7.900123478929976E-5 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 12-13 7.900123478929976E-5 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 14-15 7.900123478929976E-5 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 16-17 7.900123478929976E-5 0.0 0.0 7.900123478929976E-5 7.900123478929976E-5 18-19 7.900123478929976E-5 0.0 0.0 1.5800246957859952E-4 7.900123478929976E-5 20-21 7.900123478929976E-5 0.0 0.0 1.975030869732494E-4 7.900123478929976E-5 22-23 1.1850185218394965E-4 0.0 0.0 3.1600493915719905E-4 7.900123478929976E-5 24-25 1.5800246957859952E-4 0.0 0.0 3.950061739464988E-4 7.900123478929976E-5 26-27 1.5800246957859952E-4 0.0 0.0 7.900123478929976E-4 7.900123478929976E-5 28-29 1.5800246957859952E-4 0.0 0.0 0.0012640197566287962 7.900123478929976E-5 30-31 1.5800246957859952E-4 0.0 0.0 0.0027650432176254914 7.900123478929976E-5 32-33 1.5800246957859952E-4 0.0 0.0 0.006162096313565381 7.900123478929976E-5 34-35 1.5800246957859952E-4 0.0 0.0 0.010072657435635719 7.900123478929976E-5 36-37 1.5800246957859952E-4 0.0 0.0 0.013746214853338157 7.900123478929976E-5 38-39 1.5800246957859952E-4 0.0 0.0 0.018091282766749643 7.900123478929976E-5 40-41 1.5800246957859952E-4 0.0 0.0 0.024806387723840122 7.900123478929976E-5 42-43 1.5800246957859952E-4 0.0 0.0 0.032074501324455704 7.900123478929976E-5 44-45 1.5800246957859952E-4 0.0 0.0 0.039224113072887326 7.900123478929976E-5 46-47 1.5800246957859952E-4 0.0 0.0 0.0491782686563391 7.900123478929976E-5 48-49 1.5800246957859952E-4 0.0 0.0 0.056959890283085124 7.900123478929976E-5 50-51 1.5800246957859952E-4 0.0 0.0 0.06766455759703524 7.900123478929976E-5 52-53 2.3700370436789927E-4 0.0 0.0 0.09258944717305931 7.900123478929976E-5 54-55 2.3700370436789927E-4 0.0 0.0 0.12715248739337795 7.900123478929976E-5 56-57 2.3700370436789927E-4 0.0 0.0 0.1608465140310143 7.900123478929976E-5 58-59 2.3700370436789927E-4 0.0 0.0 0.21899142283593892 7.900123478929976E-5 60-61 2.3700370436789927E-4 0.0 0.0 0.28381193598055937 1.5800246957859952E-4 62-63 2.3700370436789927E-4 0.0 0.0 0.3269071095581224 1.5800246957859952E-4 64-65 2.3700370436789927E-4 0.0 0.0 0.3644721967004344 1.5800246957859952E-4 66-67 2.3700370436789927E-4 0.0 0.0 0.46452726056108257 1.5800246957859952E-4 68-69 2.3700370436789927E-4 0.0 0.0 0.5694804009786673 1.5800246957859952E-4 70-71 2.3700370436789927E-4 0.0 0.0 0.6159726276521702 1.5800246957859952E-4 72-73 2.3700370436789927E-4 0.0 0.0 0.7080090661817044 1.5800246957859952E-4 74-75 2.3700370436789927E-4 0.0 0.0 0.8361095683925539 1.5800246957859952E-4 76-77 2.3700370436789927E-4 0.0 0.0 0.9760997564391931 1.5800246957859952E-4 78-79 2.3700370436789927E-4 0.0 0.0 1.1096908444678992 1.5800246957859952E-4 80-81 3.1600493915719905E-4 0.0 0.0 1.2580156627848094 1.5800246957859952E-4 82-83 3.1600493915719905E-4 0.0 0.0 1.3965443279878464 1.5800246957859952E-4 84-85 3.1600493915719905E-4 0.0 0.0 1.550478233974797 1.5800246957859952E-4 86-87 3.1600493915719905E-4 0.0 0.0 1.8085752680314393 1.5800246957859952E-4 88-89 3.950061739464988E-4 0.0 0.0 2.190309234533336 1.5800246957859952E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATACCG 70 2.0547786E-4 33.924835 5 TACCTGG 1050 0.0 26.69597 2 ACCGTGC 205 9.586074E-10 25.484995 8 GTACCTG 1205 0.0 25.10002 1 GTACATG 6360 0.0 23.02307 1 GAGTACT 7580 0.0 22.240078 12-13 GTACACG 260 5.475158E-10 22.157892 1 GTGTAGG 610 0.0 22.036812 1 TACATGG 6545 0.0 21.849419 2 GTATCAA 13255 0.0 21.731617 1 ACATGGG 6480 0.0 21.481195 3 GTACTTT 7865 0.0 21.373796 14-15 AGTACTT 7705 0.0 20.554186 12-13 ATGGGCG 140 4.8557168E-4 20.3549 5 ATGGGAG 1680 0.0 20.3549 5 GTACGTG 170 7.3292504E-5 19.768314 1 TATTTCG 145 6.151073E-4 19.653006 5 GGTATCA 10375 0.0 19.203506 1 AGAGTAC 11680 0.0 19.088415 10-11 ACTTTTT 9085 0.0 18.84332 16-17 >>END_MODULE