FastQCFastQC Report
Thu 26 May 2016
SRR936665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340343
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54770.4086267470341547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44990.33566034962692387No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41480.3094730229500956No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27840.2077080269751847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26080.19457705975261555No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17530.13078741784752113No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16900.12608712844398787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG650.005832047729.243445
TACCTGG12600.026.4013142
GTACATG68450.025.5489141
GTGTACG1901.1854354E-825.0117721
GTACCTG14000.024.779521
TACATGG70300.024.2681792
ACATGGG67950.024.1283533
TAAGGTG5650.023.5500285
GAGTACT54150.022.55447612-13
ACGTATA1302.93251E-421.93342
GTATCAA120850.021.2739751
GTACTTT57700.021.04326614-15
AGTACTT57650.020.3608412-13
ATCACGC1200.00475094919.8009853
TGTACGC1200.00475094919.8009852
CATGGGG28450.019.7127154
GTAAGGT5550.019.696164
CATGGGA39450.019.637554
ACACCGT1951.0293299E-519.4948986
GGTAAGG5900.019.3311333
Produced by FastQC (version 0.11.5)