##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936665_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1340343 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.532847189115024 31.0 31.0 34.0 30.0 34.0 2 31.57426494561467 33.0 31.0 34.0 30.0 34.0 3 31.62057398740472 33.0 31.0 34.0 28.0 34.0 4 35.22995084094146 37.0 35.0 37.0 33.0 37.0 5 35.21363113770132 37.0 35.0 37.0 33.0 37.0 6 35.34431186644016 37.0 35.0 37.0 33.0 37.0 7 35.2235300964007 37.0 35.0 37.0 32.0 37.0 8 35.17725015164029 37.0 35.0 37.0 32.0 37.0 9 36.691903490375225 39.0 37.0 39.0 32.0 39.0 10-11 36.60259687259157 39.0 37.0 39.0 32.0 39.0 12-13 36.50150260045376 39.0 37.0 39.0 32.0 39.0 14-15 37.57990156251049 40.0 37.0 41.0 32.0 41.0 16-17 37.47036318315536 40.0 37.0 41.0 32.0 41.0 18-19 37.43073899740589 40.0 37.0 41.0 32.0 41.0 20-21 37.35665460259053 40.0 37.0 41.0 31.5 41.0 22-23 37.224442922445974 39.0 36.5 41.0 31.0 41.0 24-25 37.357934125817046 40.0 37.0 41.0 32.0 41.0 26-27 37.34228365425865 40.0 37.0 41.0 31.5 41.0 28-29 37.24922799611741 40.0 37.0 41.0 31.0 41.0 30-31 37.061068323555986 39.0 36.0 41.0 31.0 41.0 32-33 36.87501296309975 39.0 36.0 41.0 30.5 41.0 34-35 36.647990477064454 39.0 35.5 41.0 30.0 41.0 36-37 36.45660551067898 39.0 35.0 41.0 30.0 41.0 38-39 36.26098133089813 39.0 35.0 40.0 30.0 41.0 40-41 36.083165652374056 39.0 35.0 40.0 29.5 41.0 42-43 35.846677678773275 38.5 35.0 40.0 29.0 41.0 44-45 35.564462230936414 38.0 35.0 40.0 27.5 41.0 46-47 35.250474691925874 38.0 35.0 40.0 26.5 41.0 48-49 35.1304442221133 38.5 35.0 40.0 26.0 41.0 50-51 34.20905805454275 37.5 33.5 39.5 24.0 40.5 52-53 34.35310774928507 38.0 33.5 39.5 24.0 40.5 54-55 34.76420923599407 38.0 34.0 40.0 25.0 41.0 56-57 34.666278706271456 38.0 34.0 40.0 24.5 41.0 58-59 34.415779766820876 38.0 34.0 40.0 23.5 41.0 60-61 34.094031900789574 38.0 34.0 40.0 23.0 41.0 62-63 33.78447270586708 37.0 33.0 40.0 22.5 41.0 64-65 33.44204020911065 36.5 33.0 39.5 22.0 41.0 66-67 33.05195461161807 36.0 32.5 39.0 20.5 41.0 68-69 32.63763454578418 36.0 32.0 39.0 19.5 40.5 70-71 32.19733120551979 35.0 31.5 38.5 18.0 40.0 72-73 31.802583368585502 35.0 31.0 37.5 17.5 40.0 74-75 31.379315593098184 35.0 31.0 37.0 13.5 39.0 76-77 30.947755537202045 35.0 31.0 36.5 10.0 39.0 78-79 30.498295212494114 34.5 30.0 36.0 7.5 39.0 80-81 30.011460126251265 34.0 30.0 36.0 6.0 37.5 82-83 29.552433220451782 34.0 29.0 35.0 2.0 37.0 84-85 29.229743058306717 34.0 29.0 35.0 2.0 36.5 86-87 28.910939587851765 34.0 29.0 35.0 2.0 36.0 88-89 28.506767297624563 34.0 29.0 35.0 2.0 36.0 90-91 28.08767494589072 34.0 27.5 35.0 2.0 35.0 92-93 27.721369828469278 34.0 27.0 35.0 2.0 35.0 94-95 27.275641384332218 33.5 26.0 35.0 2.0 35.0 96-97 26.698429058830463 33.0 25.0 35.0 2.0 35.0 98-99 25.99357739026503 33.0 24.5 35.0 2.0 35.0 100-101 23.48529443582725 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39140099004118056 1101 2 0.37424528949337343 1101 3 0.3472437547721441 1101 4 0.4180495049109396 1101 5 0.36130295749467223 1101 6 0.25254040086373664 1101 7 0.41097989857195927 1101 8 0.35036377056775336 1101 9 0.4964849764361503 1101 10-11 0.4344958271161019 1101 12-13 0.5119961234262433 1101 14-15 0.6293343744919113 1101 16-17 0.6754786190378326 1101 18-19 0.7128463482248435 1101 20-21 0.6754465526559699 1101 22-23 0.5827657780172828 1101 24-25 0.5246848557045425 1101 26-27 0.35830129485730566 1101 28-29 0.4953847846475625 1101 30-31 0.4848198287814611 1101 32-33 0.4965620135602151 1101 34-35 0.4637279513923076 1101 36-37 0.3202371216513953 1101 38-39 0.3903415235806378 1101 40-41 0.41991444049513404 1101 42-43 0.3665029038642018 1101 44-45 0.49641927068861236 1101 46-47 0.550785440391401 1101 48-49 0.6534849195015582 1101 50-51 0.5772436009197293 1101 52-53 0.6659901574551199 1101 54-55 0.8774208811235127 1101 56-57 0.6924923651517005 1101 58-59 0.6458562628496836 1101 60-61 0.5207282860264755 1101 62-63 0.672201198002476 1101 64-65 0.7051407706672919 1101 66-67 0.6108214622737833 1101 68-69 0.5651179276001415 1101 70-71 0.47462537914816494 1101 72-73 0.5396414100456788 1101 74-75 0.30641712035059854 1101 76-77 0.36615085510644363 1101 78-79 0.40294266631044096 1101 80-81 0.517764885369548 1101 82-83 0.7673905840256836 1101 84-85 0.7052961550760593 1101 86-87 0.6264336688714387 1101 88-89 0.551865000527382 1101 90-91 0.4210116052557602 1101 92-93 0.4302197170824549 1101 94-95 0.6169847538373681 1101 96-97 0.3801876529350814 1101 98-99 0.20601023503609994 1101 100-101 0.9974811402337842 1102 1 0.3295155312893847 1102 2 0.22727069738891004 1102 3 0.3212767101683731 1102 4 0.3535200602154376 1102 5 0.34966672139751864 1102 6 0.2604271681187811 1102 7 0.24596549829136904 1102 8 0.21693151259027132 1102 9 0.4542769783786724 1102 10-11 0.4144140119388098 1102 12-13 0.4674834059887516 1102 14-15 0.4106952853180914 1102 16-17 0.3953179564225735 1102 18-19 0.6114774784613886 1102 20-21 0.5318558578677255 1102 22-23 0.4191462152192429 1102 24-25 0.2749273334970823 1102 26-27 0.41786382310141335 1102 28-29 0.36159639795112497 1102 30-31 0.33773091126438004 1102 32-33 0.2966014710035054 1102 34-35 0.09838102941012039 1102 36-37 0.06368752330794081 1102 38-39 -0.017806944323929486 1102 40-41 -0.01333064755904445 1102 42-43 0.23452450091264865 1102 44-45 -0.08941786787108441 1102 46-47 -0.08848146054680939 1102 48-49 0.056249638476877806 1102 50-51 0.07209592216401717 1102 52-53 0.0798215089231391 1102 54-55 0.12337126591789627 1102 56-57 0.14173356816531424 1102 58-59 0.1912289502050868 1102 60-61 0.241720994830537 1102 62-63 0.14908655558134143 1102 64-65 -0.01113727952796495 1102 66-67 0.04763188443470057 1102 68-69 -0.06710059244221611 1102 70-71 0.0015404141202779442 1102 72-73 0.00305525454962563 1102 74-75 -0.15228206554454715 1102 76-77 0.19123769520599865 1102 78-79 0.02606708878162678 1102 80-81 0.20388766883923992 1102 82-83 0.394761908918948 1102 84-85 0.25743056668801145 1102 86-87 0.2097212495568499 1102 88-89 0.3349475374049149 1102 90-91 0.29349189339626847 1102 92-93 0.06936419877352407 1102 94-95 0.2623496846013573 1102 96-97 0.029720908477674612 1102 98-99 0.3145967817341564 1102 100-101 0.3403933018946397 1103 1 0.39624818975029186 1103 2 0.4545098737399762 1103 3 0.44028762899777973 1103 4 0.4257104184164291 1103 5 0.5721204636879023 1103 6 0.35920631992529195 1103 7 0.4178065527332322 1103 8 0.5098146885928543 1103 9 0.5740867271913999 1103 10-11 0.6004758864487556 1103 12-13 0.6199638948493771 1103 14-15 0.3569514171699595 1103 16-17 0.6742783730077804 1103 18-19 0.5593285263383692 1103 20-21 0.7783154872847007 1103 22-23 0.6909053287066129 1103 24-25 0.6842436667405494 1103 26-27 0.5974479376006059 1103 28-29 0.3133563620603681 1103 30-31 0.36570968276058835 1103 32-33 0.47107190743427196 1103 34-35 0.3187788734952477 1103 36-37 0.49921072779263653 1103 38-39 0.5717631966529311 1103 40-41 0.5072387957706752 1103 42-43 0.46182850288280264 1103 44-45 0.47247670715067613 1103 46-47 0.5992164891114697 1103 48-49 0.5188884733444183 1103 50-51 0.44482625376249985 1103 52-53 0.48423681956818854 1103 54-55 0.5340460217242438 1103 56-57 0.7293266992564256 1103 58-59 0.4627762657041714 1103 60-61 0.4698267422219047 1103 62-63 0.5999298717170305 1103 64-65 0.4034683440430413 1103 66-67 0.2693531658279653 1103 68-69 0.3645905261735791 1103 70-71 0.46842824247575976 1103 72-73 0.5027678266724145 1103 74-75 0.5414222006671743 1103 76-77 0.4182439795181345 1103 78-79 0.2648078391261599 1103 80-81 0.3352957155850298 1103 82-83 0.16058278990253783 1103 84-85 0.30907309009898043 1103 86-87 0.41012313409590817 1103 88-89 0.5053445926068463 1103 90-91 0.5606171755935101 1103 92-93 0.5264614721874104 1103 94-95 0.6715118426290694 1103 96-97 0.6626391520532486 1103 98-99 0.7478937921412374 1103 100-101 1.2474734942723806 1104 1 0.204958795328249 1104 2 0.3082471156318931 1104 3 0.338883168807218 1104 4 0.29269200737549994 1104 5 0.39465035670237825 1104 6 0.12860343538211794 1104 7 0.27930548961150947 1104 8 0.28951152408998126 1104 9 0.3810595625538582 1104 10-11 0.3916326820108438 1104 12-13 0.10896880774073736 1104 14-15 0.27089812382347844 1104 16-17 0.20511408250232677 1104 18-19 0.42250178280579576 1104 20-21 0.49022245053772906 1104 22-23 0.1767480695664787 1104 24-25 0.15830271985002042 1104 26-27 -0.05993680886881947 1104 28-29 0.21072562139732298 1104 30-31 0.21743204353825973 1104 32-33 0.11866065414196214 1104 34-35 -0.0774871218896962 1104 36-37 0.06023210158933523 1104 38-39 0.31679397668126086 1104 40-41 0.07376224530930386 1104 42-43 0.17372251702840202 1104 44-45 0.2285776791106784 1104 46-47 0.3594430466948566 1104 48-49 0.3866016868726092 1104 50-51 0.413616315063571 1104 52-53 0.3821551210596752 1104 54-55 0.5560439654059905 1104 56-57 0.48900032995306475 1104 58-59 0.3531957390898768 1104 60-61 0.520971831790888 1104 62-63 0.5257172421935721 1104 64-65 0.42726232892366767 1104 66-67 0.596355680544626 1104 68-69 0.6664678877670411 1104 70-71 0.7127532961498062 1104 72-73 0.4363168921353342 1104 74-75 0.32452335650450337 1104 76-77 0.5980591698570628 1104 78-79 0.4852456743461815 1104 80-81 0.45591535627319857 1104 82-83 0.5455662279941542 1104 84-85 0.586374340723772 1104 86-87 0.7367799076445074 1104 88-89 0.7493433287739606 1104 90-91 0.8118156154850489 1104 92-93 0.8522856181290273 1104 94-95 0.7956122033911974 1104 96-97 0.8153984773529572 1104 98-99 0.9313502714861137 1104 100-101 1.256132562402815 1105 1 0.2822287643293713 1105 2 0.3467182761119112 1105 3 0.3438017848426682 1105 4 0.4029808483843951 1105 5 0.4142308979578928 1105 6 0.3384648190523478 1105 7 0.4199907198264583 1105 8 0.39429440640590485 1105 9 0.4762354753587914 1105 10-11 0.5061093151220746 1105 12-13 0.3663047478309238 1105 14-15 0.06931537170111568 1105 16-17 0.34424049578207416 1105 18-19 0.4745398228732185 1105 20-21 0.42494842510669173 1105 22-23 0.23293235059760775 1105 24-25 0.3660151305691741 1105 26-27 0.47285115134250333 1105 28-29 0.2346183917588931 1105 30-31 0.14814205727610386 1105 32-33 0.27664565764351323 1105 34-35 -0.008266387525253549 1105 36-37 0.03445741090727239 1105 38-39 -0.07614518639224599 1105 40-41 -0.1073660931731979 1105 42-43 0.029835468604879622 1105 44-45 -0.12989005340040904 1105 46-47 0.0545613505773801 1105 48-49 0.27975250105851046 1105 50-51 0.3872856614901252 1105 52-53 0.14186337147106798 1105 54-55 0.30367057302386513 1105 56-57 0.5804669379864578 1105 58-59 0.521723911194691 1105 60-61 0.1427921958308005 1105 62-63 0.1660670335289538 1105 64-65 0.275921395771654 1105 66-67 0.45991386036237003 1105 68-69 0.5475017839740275 1105 70-71 0.5596724090676233 1105 72-73 0.4533452845477477 1105 74-75 0.40055090317811803 1105 76-77 0.39154979444858284 1105 78-79 0.5824359517260014 1105 80-81 0.42156425088306193 1105 82-83 0.6989065932806007 1105 84-85 0.7140183565374407 1105 86-87 0.6548189882216882 1105 88-89 0.5467135745229754 1105 90-91 0.4768495965256072 1105 92-93 0.40598678580258607 1105 94-95 0.4093212224986651 1105 96-97 0.7328699087810264 1105 98-99 0.543114177916415 1105 100-101 0.9510316407466242 1106 1 -0.008964488040785312 1106 2 -0.01457610758706096 1106 3 0.02510379114956862 1106 4 0.01720694588282612 1106 5 0.15776105743461244 1106 6 0.05841658983890596 1106 7 0.2773516553799453 1106 8 0.28780951325925486 1106 9 0.5241884706281112 1106 10-11 0.30593805985492395 1106 12-13 0.4650654151740241 1106 14-15 0.5437466916996172 1106 16-17 0.5430230578500641 1106 18-19 0.5448816948113802 1106 20-21 0.37700835380692865 1106 22-23 0.43825646854934774 1106 24-25 0.1238706233575968 1106 26-27 0.09980989296346365 1106 28-29 0.25229990257600576 1106 30-31 0.3674800662144193 1106 32-33 -0.027280745671511397 1106 34-35 0.11814371891757247 1106 36-37 0.17604025760669373 1106 38-39 0.21784268433562914 1106 40-41 0.29336616373707614 1106 42-43 -0.040377923871240284 1106 44-45 0.3012013682261099 1106 46-47 0.296921510692691 1106 48-49 0.16552184215906607 1106 50-51 -0.13641816572805965 1106 52-53 0.1860245754376635 1106 54-55 0.46967364031264225 1106 56-57 0.3534302977725403 1106 58-59 0.40091140403582415 1106 60-61 0.5120464777193021 1106 62-63 0.4896431922008375 1106 64-65 0.5594464262063283 1106 66-67 0.5801017089927853 1106 68-69 0.5354703285525986 1106 70-71 0.763538145749088 1106 72-73 0.6789498982528279 1106 74-75 0.5011259944821624 1106 76-77 0.6792573762066283 1106 78-79 0.7183743180754298 1106 80-81 0.678546488787493 1106 82-83 0.8061382891385449 1106 84-85 0.8720093229361332 1106 86-87 1.183019089788882 1106 88-89 1.2841740297102504 1106 90-91 1.3161775399723474 1106 92-93 1.3398147946294081 1106 94-95 1.1422932590831287 1106 96-97 1.2476526492504867 1106 98-99 1.752080067759593 1106 100-101 2.1934229704355666 1107 1 0.08385496216569877 1107 2 0.1653703958464945 1107 3 0.08562122282235052 1107 4 0.1706752208757507 1107 5 0.36921562952358045 1107 6 0.24672584561712796 1107 7 0.4732006394036645 1107 8 0.3267678510189569 1107 9 0.2875539458853922 1107 10-11 0.29550041455370746 1107 12-13 0.39906665418313025 1107 14-15 0.24779376733620495 1107 16-17 0.1088770068561189 1107 18-19 0.3569353076747035 1107 20-21 0.5281042841987684 1107 22-23 0.5551244928330377 1107 24-25 0.5088099532862671 1107 26-27 0.35892494077378245 1107 28-29 0.43179044394918265 1107 30-31 0.3947455350977336 1107 32-33 0.45579302569105096 1107 34-35 0.4690245559867563 1107 36-37 0.41379190337532634 1107 38-39 0.45666420552122844 1107 40-41 0.49057939966431263 1107 42-43 0.5838338572267148 1107 44-45 0.5508843473944367 1107 46-47 0.6359604735034097 1107 48-49 0.6026292702870322 1107 50-51 0.44697396835330494 1107 52-53 0.8209815176347561 1107 54-55 0.6852875033484622 1107 56-57 0.8577883557487738 1107 58-59 0.7934634273220382 1107 60-61 0.7408524889263006 1107 62-63 0.7335241869417573 1107 64-65 0.7730164274252118 1107 66-67 0.7247982356297982 1107 68-69 0.791479466803878 1107 70-71 0.831251726653143 1107 72-73 0.7456366559516319 1107 74-75 0.7537479859992011 1107 76-77 0.7061510305342686 1107 78-79 0.8934835980076201 1107 80-81 0.8269432691530021 1107 82-83 0.9347632014965406 1107 84-85 0.8940126373973207 1107 86-87 0.7946347442440036 1107 88-89 0.7303470235610554 1107 90-91 0.7702936668835569 1107 92-93 0.833452851889966 1107 94-95 0.6618473499937458 1107 96-97 0.8150131461065548 1107 98-99 1.283701078580794 1107 100-101 1.5815856250461202 1108 1 -0.23402120195700604 1108 2 0.04046709613510657 1108 3 0.08607125108859037 1108 4 0.1894263986356748 1108 5 0.3292399781603592 1108 6 0.20709856324236853 1108 7 0.01461811686505854 1108 8 0.1500133606542704 1108 9 0.21246121278810648 1108 10-11 -0.010032205676985484 1108 12-13 0.2567281684600573 1108 14-15 0.22813013557090045 1108 16-17 0.11375169320005796 1108 18-19 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-1.4527866877835791 2316 58-59 -1.8101045339061912 2316 60-61 -1.1631928315733475 2316 62-63 -1.02467017688722 2316 64-65 -1.4022309066247658 2316 66-67 -1.4204345485928727 2316 68-69 -1.6092557029137424 2316 70-71 -1.7749106067331155 2316 72-73 -1.3816541609990516 2316 74-75 -1.1673536884022155 2316 76-77 -1.3371002181740756 2316 78-79 -2.129117437398694 2316 80-81 -1.689023799824632 2316 82-83 -1.486825202369186 2316 84-85 -1.1372141811358816 2316 86-87 -1.5769448099950978 2316 88-89 -2.1632531346910895 2316 90-91 -1.8050173063931751 2316 92-93 -2.5452083557717273 2316 94-95 -3.2819379997096654 2316 96-97 -3.921956429397678 2316 98-99 -5.093079583423332 2316 100-101 -5.603729460704155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2944.0 3 1158.0 4 1132.0 5 1231.0 6 1331.0 7 1527.0 8 1951.0 9 2470.0 10 3148.0 11 3972.0 12 5025.0 13 6190.0 14 7277.0 15 8886.0 16 11103.0 17 14055.0 18 11871.0 19 8338.0 20 8327.0 21 8855.0 22 9594.0 23 10973.0 24 12542.0 25 14239.0 26 16983.0 27 19920.0 28 23753.0 29 28575.0 30 35353.0 31 43389.0 32 55261.0 33 71157.0 34 95117.0 35 131333.0 36 185254.0 37 245980.0 38 198894.0 39 31226.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.99625332871592 18.514900108668396 14.17219462389988 22.316651938715804 2 15.584213994508945 20.15840333970897 40.06307476807784 24.19430789770425 3 19.96744912152925 24.527407809822872 29.636895771519995 25.868247297127883 4 12.676027949475948 16.29423094150318 37.4319925945833 33.597748514437576 5 13.46199356922785 37.614641914091635 33.95588045363297 14.967484063047548 6 31.562972617485336 35.88108216247367 17.524675018326175 15.031270201714822 7 28.609339052359545 33.04073835753668 20.36574496387787 17.984177626225907 8 26.640178485456257 33.84931295191447 19.60421492001669 19.90629364261258 9 26.500489663326604 16.902789886660855 20.823007607696074 35.77371284231647 10-11 25.13859594183468 26.542676501973915 26.88378693419021 21.434940622001193 12-13 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29.632893319658997 21.896422499680092 38-39 21.849602595441265 26.636439744634366 29.642977594322023 21.870980065602343 40-41 22.018036838423875 26.66978782933085 29.53926789461413 21.77290743763115 42-43 21.835862832551676 26.850046033851665 29.008265202735473 22.305825930861182 44-45 21.98606747508239 26.748821360916857 28.98135291874982 22.283758245250937 46-47 22.350991709917622 27.07681988814003 28.552344877573653 22.019843524368692 48-49 22.342905467789127 27.239641834874433 28.296022290008104 22.121430407328337 50-51 21.973914023115082 27.440598894158708 28.424120202605764 22.161366880120447 52-53 22.406426009641102 27.624686358233923 27.86407252688385 22.10481510524113 54-55 21.967291274638985 27.34571208144128 28.138509566097174 22.548487077822564 56-57 22.26856942236423 27.5134847344635 27.882964779817428 22.33498106335484 58-59 22.639513839741564 27.251306038986982 27.809685972378347 22.29949414889311 60-61 22.19181649415613 27.25882848035529 28.63327931186801 21.91607571362057 62-63 22.0716096121146 27.418454681044896 28.447279534600195 22.06265617224031 64-65 21.78831969751004 27.77455306183049 28.13580414550913 22.30132309515034 66-67 21.905836338389886 27.634694058755105 27.798395960129557 22.661073642725448 68-69 21.80719066059591 27.50007368932803 28.205476441615108 22.48725920846095 70-71 22.072433706892692 27.51178875740528 27.90421261546267 22.51156492023936 72-73 22.084371966637395 27.280137401766613 28.217985037711625 22.417505593884368 74-75 21.781235241056223 28.093717101311732 27.821872745355662 22.303174912276383 76-77 22.00688455444577 27.580709966412897 27.750492166926012 22.66191331221533 78-79 22.08139495842209 27.62996593931752 27.854661985965357 22.433977116295033 80-81 22.12700326671074 27.771779439570327 27.657578873380263 22.44363842033867 82-83 22.031487943748186 27.768699930386585 27.868829830652135 22.33098229521309 84-85 21.905270392184516 27.861390379439282 27.68181543441616 22.551523793960044 86-87 21.91620867847214 27.92527990378144 27.614076739074317 22.5444346786721 88-89 21.870839191962453 27.858560226582984 27.289118264037672 22.981482317416887 90-91 21.956127437996674 27.52047876170175 27.675967383202604 22.847426417098973 92-93 22.15551480396016 27.639108348459718 27.731303132794444 22.47407371478568 94-95 21.82693563250124 27.34008856920075 27.902133644730803 22.930842153567205 96-97 21.52686399724818 27.335753610736273 28.18319890733093 22.954183484684613 98-99 21.53736570885093 27.569175822761245 27.635255668557523 23.258202799830304 100-101 21.440625466487536 27.417898193760266 27.880728466935363 23.26074787281684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 182.0 1 132.0 2 160.0 3 242.5 4 244.0 5 249.0 6 380.5 7 760.0 8 1259.5 9 1555.5 10 1486.5 11 1358.5 12 1437.5 13 1619.5 14 1748.5 15 1831.0 16 1958.0 17 2096.5 18 2204.0 19 2418.5 20 2714.0 21 3068.5 22 3696.0 23 4409.5 24 5207.0 25 6453.0 26 8139.5 27 10039.0 28 11872.5 29 13894.0 30 16577.0 31 19622.0 32 22716.5 33 25298.0 34 27804.0 35 29916.0 36 32389.0 37 35016.0 38 36420.0 39 37359.5 40 38532.5 41 40704.5 42 42414.5 43 44043.5 44 45992.0 45 48074.5 46 49020.0 47 51606.0 48 60040.5 49 59651.5 50 51069.0 51 46559.5 52 43590.5 53 42117.5 54 40984.0 55 38205.0 56 34955.0 57 31664.5 58 28845.5 59 25595.0 60 21440.0 61 17900.0 62 14891.5 63 11704.0 64 8703.5 65 6747.5 66 5051.5 67 3854.0 68 2808.5 69 1835.5 70 1359.5 71 1046.0 72 847.5 73 719.0 74 555.0 75 433.0 76 317.5 77 202.5 78 134.0 79 85.5 80 53.5 81 42.5 82 27.5 83 16.0 84 15.0 85 9.5 86 3.5 87 5.5 88 5.0 89 2.5 90 3.0 91 2.5 92 2.5 93 3.0 94 1.5 95 1.0 96 2.0 97 1.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036333983166995314 2 0.025814287835277984 3 0.02141242950498492 4 0.0584178825867707 5 0.06311817199030398 6 0.054389063098027886 7 0.05379220095154748 8 0.04670446296209254 9 0.048271226096603635 10-11 0.055769306811763855 12-13 0.048271226096603635 14-15 0.042712947357504756 16-17 0.05121823294485068 18-19 0.05550817962267867 20-21 0.012869840033483966 22-23 0.02062904793772937 24-25 0.005334455434168717 26-27 0.01070621475249246 28-29 0.0061924447697343146 30-31 0.009437882691221575 32-33 0.003357349573952339 34-35 0.007945727325020536 36-37 0.008915628313051212 38-39 0.01096734194157764 40-41 0.0022755369334565853 42-43 0.0018651942077512996 44-45 0.0019398019760613514 46-47 4.8495049401533786E-4 48-49 8.579893355655979E-4 50-51 4.476466098603119E-4 52-53 0.002984310732402079 54-55 0.0066400913795946264 56-57 0.016152581839126254 58-59 0.009437882691221575 60-61 0.010072048721857017 62-63 0.005670190391563951 64-65 7.460776831005199E-5 66-67 0.007609992367625301 68-69 0.019211500339838383 70-71 0.0061924447697343146 72-73 0.002984310732402079 74-75 0.004028819488742807 76-77 0.007199649641920016 78-79 0.006565483611284574 80-81 0.01171341962467816 82-83 0.006267052538044366 84-85 0.01745821778455216 86-87 0.004551073866913171 88-89 0.0062297486538893405 90-91 0.003879603952122703 92-93 0.01831620712011776 94-95 0.011041949709887693 96-97 0.010333175910942198 98-99 0.022195811072240463 100-101 0.04051201819235822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1340343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.86103342345768 #Duplication Level Percentage of deduplicated Percentage of total 1 82.35582563675517 51.76972307968519 2 10.784073631741498 13.557960260118618 3 2.9675169244402144 5.596235417157379 4 1.2364479728119324 3.108975893811895 5 0.674976807981582 2.1214869843294504 6 0.41023486046989643 1.5472672365279394 7 0.273543157623742 1.2036643901900936 8 0.19922338737973802 1.0018710410249736 9 0.1470506906501937 0.8319382541913927 >10 0.8497285789080655 9.853586558662473 >50 0.0654267541952114 2.7983153950137485 >100 0.032975503319050053 3.839812488512897 >500 0.0017856562342036149 0.784240730931272 >1k 0.001071393740522169 1.5750666893741807 >5k 1.1904374894690767E-4 0.40985558046856646 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5477 0.4086267470341547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4499 0.33566034962692387 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4148 0.3094730229500956 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2784 0.2077080269751847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2608 0.19457705975261555 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1753 0.13078741784752113 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1690 0.12608712844398787 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.460776831005199E-5 0.0 0.0 0.0 0.0 7 7.460776831005199E-5 0.0 0.0 0.0 0.0 8 7.460776831005199E-5 0.0 0.0 0.0 0.0 9 7.460776831005199E-5 0.0 0.0 0.0 0.0 10-11 7.460776831005199E-5 0.0 0.0 3.730388415502599E-5 0.0 12-13 7.460776831005199E-5 0.0 0.0 7.460776831005199E-5 0.0 14-15 7.460776831005199E-5 0.0 0.0 7.460776831005199E-5 0.0 16-17 7.460776831005199E-5 0.0 0.0 7.460776831005199E-5 0.0 18-19 7.460776831005199E-5 0.0 0.0 7.460776831005199E-5 0.0 20-21 7.460776831005199E-5 0.0 0.0 1.1191165246507797E-4 0.0 22-23 7.460776831005199E-5 0.0 0.0 1.4921553662010397E-4 3.730388415502599E-5 24-25 1.1191165246507797E-4 0.0 0.0 2.9843107324020794E-4 7.460776831005199E-5 26-27 1.4921553662010397E-4 0.0 0.0 7.087737989454937E-4 7.460776831005199E-5 28-29 1.8651942077512997E-4 0.0 0.0 0.0015294592503560657 7.460776831005199E-5 30-31 2.2382330493015594E-4 0.0 0.0 0.003320045689797313 7.460776831005199E-5 32-33 3.730388415502599E-4 0.0 0.0 0.006378964190509444 7.460776831005199E-5 34-35 4.476466098603119E-4 0.0 0.0 0.009885529301081887 7.460776831005199E-5 36-37 5.222543781703638E-4 0.0 0.0 0.01357861383242946 7.460776831005199E-5 38-39 5.222543781703638E-4 0.0 0.0 0.017607433321172268 7.460776831005199E-5 40-41 5.222543781703638E-4 0.0 0.0 0.023949093627526685 7.460776831005199E-5 42-43 5.222543781703638E-4 0.0 0.0 0.02988041120817582 7.460776831005199E-5 44-45 5.222543781703638E-4 0.0 0.0 0.03711736473425086 7.460776831005199E-5 46-47 5.222543781703638E-4 0.0 0.0 0.046890982382867666 7.460776831005199E-5 48-49 5.222543781703638E-4 0.0 0.0 0.05487401359204323 7.460776831005199E-5 50-51 5.222543781703638E-4 0.0 0.0 0.06722159924735682 7.460776831005199E-5 52-53 5.222543781703638E-4 0.0 0.0 0.09340892592418507 7.460776831005199E-5 54-55 5.222543781703638E-4 0.0 0.0 0.12728085273694867 7.460776831005199E-5 56-57 5.222543781703638E-4 0.0 0.0 0.1657784611849355 7.460776831005199E-5 58-59 5.222543781703638E-4 0.0 0.0 0.2237486971618459 7.460776831005199E-5 60-61 5.222543781703638E-4 0.0 0.0 0.2922386284704736 7.460776831005199E-5 62-63 5.222543781703638E-4 0.0 0.0 0.33692868168819473 7.460776831005199E-5 64-65 5.222543781703638E-4 0.0 0.0 0.37542629013618156 7.460776831005199E-5 66-67 5.222543781703638E-4 0.0 0.0 0.4745054064519306 7.460776831005199E-5 68-69 5.222543781703638E-4 0.0 0.0 0.5816048578610102 7.460776831005199E-5 70-71 5.222543781703638E-4 0.0 0.0 0.6284585363597228 7.460776831005199E-5 72-73 5.222543781703638E-4 0.0 0.0 0.716197271892344 1.4921553662010397E-4 74-75 5.222543781703638E-4 0.0 0.0 0.849670569399027 1.4921553662010397E-4 76-77 5.222543781703638E-4 0.0 0.0 0.9872099902786078 1.4921553662010397E-4 78-79 5.222543781703638E-4 0.0 0.0 1.1244509800849485 1.4921553662010397E-4 80-81 5.222543781703638E-4 0.0 0.0 1.2727712234853317 1.4921553662010397E-4 82-83 5.222543781703638E-4 0.0 0.0 1.407997803547301 2.2382330493015594E-4 84-85 5.222543781703638E-4 0.0 0.0 1.5609437285829075 2.2382330493015594E-4 86-87 5.222543781703638E-4 0.0 0.0 1.8202803312286482 2.2382330493015594E-4 88-89 5.968621464804159E-4 0.0 0.0 2.20335391761661 2.2382330493015594E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACCG 65 0.0058320477 29.24344 5 TACCTGG 1260 0.0 26.401314 2 GTACATG 6845 0.0 25.548914 1 GTGTACG 190 1.1854354E-8 25.011772 1 GTACCTG 1400 0.0 24.77952 1 TACATGG 7030 0.0 24.268179 2 ACATGGG 6795 0.0 24.128353 3 TAAGGTG 565 0.0 23.550028 5 GAGTACT 5415 0.0 22.554476 12-13 ACGTATA 130 2.93251E-4 21.9334 2 GTATCAA 12085 0.0 21.273975 1 GTACTTT 5770 0.0 21.043266 14-15 AGTACTT 5765 0.0 20.36084 12-13 ATCACGC 120 0.004750949 19.800985 3 TGTACGC 120 0.004750949 19.800985 2 CATGGGG 2845 0.0 19.712715 4 GTAAGGT 555 0.0 19.69616 4 CATGGGA 3945 0.0 19.63755 4 ACACCGT 195 1.0293299E-5 19.494898 6 GGTAAGG 590 0.0 19.331133 3 >>END_MODULE