FastQCFastQC Report
Thu 26 May 2016
SRR936665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340343
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76270.5690334489007665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68490.510988605155546No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51750.386095201004519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40370.3011915606676798No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24760.1847288343356887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.15525876585321818No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16550.12347585655313603No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14000.10445087563407277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG66900.024.2201061
TACATGG68450.023.5951982
TACCTGG12300.022.7858432
GTACCTG12350.022.7003671
ACATGGG67700.022.4524273
GAGTACT78150.022.30647712-13
GTACTTT81800.021.16478214-15
CATGGGG28600.020.9254154
GGTATCA115200.020.4174041
AGTACTT79950.019.96248812-13
GTATCAA143950.019.7725641
AGAGTAC123000.018.69211410-11
ACTTTTT93600.018.6741816-17
CATGGGA38850.018.3387534
GTATAGG3408.8766683E-1018.1682131
GTATAGA4500.017.9508171
TACTTTT90550.017.57219114-15
TAAGGTG4900.017.4480135
TCAACGC167550.016.8955144
ATCAACG168250.016.8547083
Produced by FastQC (version 0.11.5)