##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936665_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1340343 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92231540732484 31.0 31.0 34.0 30.0 34.0 2 32.05665340886624 31.0 31.0 34.0 30.0 34.0 3 32.153393571645466 33.0 31.0 34.0 30.0 34.0 4 35.89787912497025 37.0 35.0 37.0 35.0 37.0 5 35.68644742427871 37.0 35.0 37.0 33.0 37.0 6 35.80643238335262 37.0 35.0 37.0 35.0 37.0 7 35.75171280784098 37.0 35.0 37.0 33.0 37.0 8 35.731788803313776 37.0 35.0 37.0 33.0 37.0 9 37.364172454364294 39.0 37.0 39.0 34.0 39.0 10-11 37.23698411526005 39.0 37.0 39.0 33.5 39.0 12-13 37.139924258193616 39.0 37.0 39.0 33.0 39.0 14-15 38.22636593767416 40.0 38.0 41.0 33.0 41.0 16-17 38.1639833982794 40.0 37.5 41.0 33.0 41.0 18-19 38.33578830194958 40.0 38.0 41.0 33.5 41.0 20-21 38.379712879464435 40.0 38.0 41.0 34.0 41.0 22-23 38.29074274271586 40.0 38.0 41.0 34.0 41.0 24-25 38.1639681036869 40.0 37.5 41.0 33.0 41.0 26-27 37.944611565845456 40.0 37.0 41.0 33.0 41.0 28-29 37.85357367479817 40.0 37.0 41.0 33.0 41.0 30-31 37.79764918382831 40.0 37.0 41.0 33.0 41.0 32-33 37.61810409723481 40.0 36.5 41.0 33.0 41.0 34-35 37.427332033666005 40.0 36.0 41.0 32.5 41.0 36-37 37.15496854163449 39.0 36.0 41.0 31.5 41.0 38-39 36.97601621376021 39.0 36.0 41.0 31.0 41.0 40-41 36.738860500633045 39.0 35.0 41.0 31.0 41.0 42-43 36.464252060853084 39.0 35.0 40.5 30.0 41.0 44-45 36.160346269574276 39.0 35.0 40.0 30.0 41.0 46-47 35.7218215785064 39.0 35.0 40.0 28.5 41.0 48-49 35.28828031332279 38.0 35.0 40.0 27.0 41.0 50-51 34.9385481925149 38.0 34.0 40.0 26.0 41.0 52-53 34.65251991467856 38.0 34.0 40.0 24.5 41.0 54-55 34.37403448221836 38.0 34.0 40.0 24.0 41.0 56-57 34.053634032482734 38.0 33.0 40.0 23.0 41.0 58-59 33.714928193753394 37.0 33.0 40.0 22.5 41.0 60-61 33.33920459166049 37.0 32.5 39.5 22.0 41.0 62-63 32.94866164854817 36.0 32.0 39.0 20.5 40.0 64-65 32.53418863678924 36.0 31.5 39.0 19.5 40.0 66-67 32.074843901896756 35.0 31.0 38.5 18.0 40.0 68-69 31.78632074028812 35.0 31.0 38.0 17.5 40.0 70-71 31.352385173048987 35.0 30.5 37.5 14.0 39.5 72-73 30.903757471035398 34.5 30.0 37.0 10.0 39.0 74-75 30.384266191564397 34.0 29.5 36.5 7.5 39.0 76-77 28.997296214476442 32.5 28.0 35.0 7.0 37.0 78-79 29.76194041375976 34.0 29.0 35.0 6.5 37.5 80-81 29.556209865683634 34.0 29.0 35.0 2.0 37.0 82-83 29.20375530741012 34.0 29.0 35.0 2.0 37.0 84-85 28.780130160712595 34.0 29.0 35.0 2.0 36.0 86-87 28.40636874292625 33.5 28.5 35.0 2.0 36.0 88-89 27.992106498112797 33.0 27.5 35.0 2.0 35.0 90-91 27.497751321863134 33.0 26.5 35.0 2.0 35.0 92-93 27.005205757033835 33.0 25.5 34.5 2.0 35.0 94-95 26.407765773387858 32.0 25.0 34.0 2.0 35.0 96-97 25.626293791962205 32.0 23.5 34.0 2.0 35.0 98-99 24.660506303237305 32.0 13.0 34.0 2.0 35.0 100-101 21.461652353166315 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25849392923704073 1101 2 0.2727468256540675 1101 3 0.2652162626501351 1101 4 0.12033707710208574 1101 5 0.1362254165746677 1101 6 0.1869212919432499 1101 7 0.11198187836200191 1101 8 0.06380048031604701 1101 9 0.2837874095499444 1101 10-11 0.23930196944153437 1101 12-13 0.0880062652693212 1101 14-15 0.2728971534371354 1101 16-17 0.4589340976455034 1101 18-19 0.33677466929524513 1101 20-21 0.2742923841310585 1101 22-23 0.2526475073530321 1101 24-25 0.23581925468867126 1101 26-27 0.2378594210149103 1101 28-29 0.09999752251856364 1101 30-31 0.13623710077594353 1101 32-33 0.2319925543541288 1101 34-35 0.2623289646687397 1101 36-37 0.17800652104203607 1101 38-39 0.13062798745335868 1101 40-41 0.21396970671354865 1101 42-43 0.19988726930014167 1101 44-45 0.194615720572763 1101 46-47 -0.027326007608337477 1101 48-49 0.11022036075939212 1101 50-51 0.14217219829105687 1101 52-53 0.14332693192207557 1101 54-55 0.050727221621954754 1101 56-57 0.08906132269449785 1101 58-59 0.08694509794130312 1101 60-61 0.1812583207566405 1101 62-63 0.2956636336815279 1101 64-65 0.16953521234822233 1101 66-67 0.0739172885720194 1101 68-69 0.07398423432098511 1101 70-71 0.10208261766410232 1101 72-73 0.05843096423015126 1101 74-75 0.188995647275366 1101 76-77 0.12798110803364438 1101 78-79 -0.08270246394043923 1101 80-81 0.22808947132971724 1101 82-83 0.25020319498286625 1101 84-85 0.30511402424997414 1101 86-87 0.22116845264974572 1101 88-89 0.04078694219943557 1101 90-91 0.17434514714839722 1101 92-93 0.20500341233470465 1101 94-95 0.415975556916937 1101 96-97 0.5496007554154723 1101 98-99 0.9057739369051063 1101 100-101 0.9790425523088899 1102 1 0.25693114468817413 1102 2 0.25393338179492275 1102 3 0.2594912236985607 1102 4 0.21659772555312884 1102 5 0.13147366122431237 1102 6 0.15621058582363645 1102 7 0.22126887898927805 1102 8 0.25994916405620927 1102 9 0.31229322024864814 1102 10-11 0.3539305636099286 1102 12-13 0.17354157124999148 1102 14-15 0.3614170608976295 1102 16-17 0.2529793961393736 1102 18-19 0.19103708490015947 1102 20-21 0.23089560412822152 1102 22-23 0.2667550609240976 1102 24-25 0.2702295176708489 1102 26-27 0.03924279223188876 1102 28-29 0.10982748454499358 1102 30-31 0.29543641819589794 1102 32-33 0.2900836909308424 1102 34-35 0.3215940444941907 1102 36-37 0.4731465106549422 1102 38-39 0.4345730572964044 1102 40-41 0.4472859530628952 1102 42-43 0.4863934981802913 1102 44-45 0.5038305511755468 1102 46-47 0.5426489015046485 1102 48-49 0.41516164673480205 1102 50-51 0.40570677582662285 1102 52-53 0.4016352530584584 1102 54-55 0.5521008127888862 1102 56-57 0.49779659360188333 1102 58-59 0.3642122147151028 1102 60-61 0.4407365382244848 1102 62-63 0.356306014348057 1102 64-65 0.2774465983499397 1102 66-67 0.30416057574801414 1102 68-69 0.20055292062419028 1102 70-71 0.3662019747438521 1102 72-73 0.30173837481014587 1102 74-75 0.19762772179260324 1102 76-77 -0.10227229643550118 1102 78-79 -0.08711731342884121 1102 80-81 0.3232564403910345 1102 82-83 0.30209715226090594 1102 84-85 0.23420495173596123 1102 86-87 0.3503946146208641 1102 88-89 0.4804612328085618 1102 90-91 0.7631817258845466 1102 92-93 0.6172844572487399 1102 94-95 0.6465517030376269 1102 96-97 1.0385439029728225 1102 98-99 0.8739955191148603 1102 100-101 1.1602746362689373 1103 1 0.14985637159499277 1103 2 0.20726545291514498 1103 3 0.2430025359405832 1103 4 0.11209676963248683 1103 5 0.03410935427877604 1103 6 0.213872116517706 1103 7 0.23904756226776414 1103 8 0.22200765528622668 1103 9 0.26548603633527534 1103 10-11 0.15913695260342564 1103 12-13 0.21242270850400757 1103 14-15 0.16624924486244197 1103 16-17 0.32972023840594744 1103 18-19 0.17392809485586014 1103 20-21 0.32475606921259725 1103 22-23 0.25166389689607627 1103 24-25 0.18928032655052363 1103 26-27 0.30644267533997294 1103 28-29 0.290716514746876 1103 30-31 0.17388632445599939 1103 32-33 0.3102912769688473 1103 34-35 0.40881742946164223 1103 36-37 0.5004171737352792 1103 38-39 0.4964383064428688 1103 40-41 0.391520314482527 1103 42-43 0.3564157309666882 1103 44-45 0.4456751110027355 1103 46-47 0.3794447460795709 1103 48-49 0.3146740483216277 1103 50-51 0.5285717320573227 1103 52-53 0.4291504699293043 1103 54-55 0.3256307975531527 1103 56-57 0.3214832574285751 1103 58-59 0.5006296697504737 1103 60-61 0.37381506228576455 1103 62-63 0.43342414857755784 1103 64-65 0.1868538246572058 1103 66-67 0.40164630210706775 1103 68-69 0.46372641250246716 1103 70-71 0.4199220048997674 1103 72-73 0.24199350649533002 1103 74-75 0.3797314606187605 1103 76-77 0.2631598366291996 1103 78-79 0.21829898709312445 1103 80-81 0.41418843385771 1103 82-83 0.49193383285993875 1103 84-85 0.4472323973253154 1103 86-87 0.22158422303982306 1103 88-89 0.2534173907964856 1103 90-91 0.20615404568819073 1103 92-93 0.462970843937331 1103 94-95 0.40511863154491934 1103 96-97 0.15730975352267507 1103 98-99 0.5392036093521178 1103 100-101 0.5837538238152824 1104 1 0.08005291314738017 1104 2 0.15751239239360615 1104 3 0.08241288538519598 1104 4 0.003231033747660206 1104 5 -0.07315846175066554 1104 6 -0.018631696994425795 1104 7 0.1889889028111611 1104 8 0.04712477996282871 1104 9 0.05715561655111401 1104 10-11 0.1146658329977086 1104 12-13 0.05835552335508254 1104 14-15 0.14052066333356805 1104 16-17 0.16230899806274834 1104 18-19 0.09350118868250235 1104 20-21 -0.01383589664320084 1104 22-23 0.0465992420027348 1104 24-25 8.863525471483058E-4 1104 26-27 0.1533143004788613 1104 28-29 0.03288165730359083 1104 30-31 0.07085934110595815 1104 32-33 -0.0375709548875065 1104 34-35 -0.04305850773000941 1104 36-37 0.19500470581669305 1104 38-39 0.02520086383978537 1104 40-41 0.033882801436305954 1104 42-43 0.1377454489092358 1104 44-45 0.051136526547360006 1104 46-47 0.06988985869795528 1104 48-49 0.12089454054309812 1104 50-51 0.01640345581660796 1104 52-53 0.2606139521514734 1104 54-55 0.28237689404472377 1104 56-57 0.4778942310201657 1104 58-59 0.34832226829965407 1104 60-61 0.25001771063720923 1104 62-63 0.1974370251699753 1104 64-65 0.37952877357297155 1104 66-67 0.16858438206521953 1104 68-69 0.42830930880342777 1104 70-71 0.06970303549332613 1104 72-73 -0.07187602347797295 1104 74-75 0.17295363881401826 1104 76-77 0.22889908822258676 1104 78-79 0.274776519096843 1104 80-81 0.2660804279425548 1104 82-83 0.14152321666371392 1104 84-85 0.24153712090071977 1104 86-87 0.22976676401727403 1104 88-89 0.3242334694441844 1104 90-91 0.49842834922045043 1104 92-93 0.3131535558429981 1104 94-95 0.19129649530477266 1104 96-97 0.4155874704396467 1104 98-99 0.5056395229246 1104 100-101 0.7009031037154188 1105 1 0.07626829693706938 1105 2 0.2191032989269175 1105 3 0.12747942347417762 1105 4 -0.0613407341580654 1105 5 -0.07439547377366296 1105 6 -0.022162678129348024 1105 7 0.07672335294676458 1105 8 0.0771636562469098 1105 9 0.09401234216557697 1105 10-11 0.1064211781621438 1105 12-13 -0.05291214994563376 1105 14-15 0.021245739359166294 1105 16-17 0.17438479772739868 1105 18-19 0.11878960119528159 1105 20-21 0.1986624694448409 1105 22-23 0.12961517080486118 1105 24-25 0.1141831064510086 1105 26-27 0.1088026432203506 1105 28-29 0.2297345048117876 1105 30-31 0.24445004334616982 1105 32-33 0.37809062147612593 1105 34-35 0.3046336294960952 1105 36-37 0.17414235521684418 1105 38-39 0.3387379651111644 1105 40-41 0.42276943002019607 1105 42-43 0.3819618333888428 1105 44-45 0.5258745163205916 1105 46-47 0.44381439836760705 1105 48-49 0.44536240463278176 1105 50-51 0.4602912400415491 1105 52-53 0.5749967922237431 1105 54-55 0.4595905525965023 1105 56-57 0.47256984214651965 1105 58-59 0.6378860938783575 1105 60-61 0.4949534492043739 1105 62-63 0.5760851935005604 1105 64-65 0.5474148507386332 1105 66-67 0.4640398128423229 1105 68-69 0.3695090373359875 1105 70-71 0.3414808096161437 1105 72-73 0.5352181409243038 1105 74-75 0.30537819969206126 1105 76-77 0.19724707554602716 1105 78-79 0.4546726066243174 1105 80-81 0.3569609917915102 1105 82-83 0.4549471343647582 1105 84-85 0.33406306655702167 1105 86-87 0.3262340083024782 1105 88-89 0.47975180691387465 1105 90-91 0.4777060173012977 1105 92-93 0.4902217854095774 1105 94-95 0.5423198652233019 1105 96-97 0.5230103649439037 1105 98-99 0.7143526235657838 1105 100-101 0.7646501248017543 1106 1 0.03824141710768458 1106 2 0.010272223824969728 1106 3 0.13495910750086182 1106 4 0.048696983560141405 1106 5 -0.010647360419802965 1106 6 0.053629561204324716 1106 7 0.06369447116915694 1106 8 0.061136920355068014 1106 9 -0.04499888800373242 1106 10-11 -0.01783315947680819 1106 12-13 0.08612264746227538 1106 14-15 0.10185000335683725 1106 16-17 0.20419608749901386 1106 18-19 0.02021037624827926 1106 20-21 0.06086127760143967 1106 22-23 0.15294546996180713 1106 24-25 0.07730037804392254 1106 26-27 0.11474868285993978 1106 28-29 0.11337772621509146 1106 30-31 0.09382396571518115 1106 32-33 0.1952590253608193 1106 34-35 0.19722530605423572 1106 36-37 0.3182970718575433 1106 38-39 0.46513286688393407 1106 40-41 0.3314719749778732 1106 42-43 0.2188968151787165 1106 44-45 0.12713432459983665 1106 46-47 0.1947170489470551 1106 48-49 0.18735157079854048 1106 50-51 0.056894618113773276 1106 52-53 -0.11027971483781585 1106 54-55 0.05402802541815532 1106 56-57 0.1790784038962272 1106 58-59 0.1173415436882479 1106 60-61 0.22699952138195556 1106 62-63 0.08863563499350136 1106 64-65 0.2099563214762341 1106 66-67 0.15399276959314534 1106 68-69 0.2656821739276438 1106 70-71 0.2422500645568988 1106 72-73 0.11838768617289475 1106 74-75 0.1963959394874486 1106 76-77 0.009610296868245882 1106 78-79 -0.038810164964033333 1106 80-81 0.12145889810843968 1106 82-83 0.45785097293575916 1106 84-85 0.15693651606079229 1106 86-87 0.3772631617368596 1106 88-89 0.3594101377046499 1106 90-91 0.5378850877688599 1106 92-93 0.18899117009685185 1106 94-95 0.6921317327723813 1106 96-97 0.904248460954296 1106 98-99 1.3017926538129423 1106 100-101 1.380993472606555 1107 1 0.01856655635642923 1107 2 0.10310285642410832 1107 3 0.007494368380484673 1107 4 0.01772846535017436 1107 5 -0.1209605456908065 1107 6 -0.025091128346737435 1107 7 -0.08609367840205095 1107 8 -0.09544061891470079 1107 9 0.037860405067768 1107 10-11 -0.011027834868720277 1107 12-13 -3.7120278088309533E-4 1107 14-15 0.09383446430400966 1107 16-17 0.01315357539686346 1107 18-19 -0.07536581330669634 1107 20-21 0.046323048914800324 1107 22-23 0.06231665047582169 1107 24-25 -0.0024476938320319164 1107 26-27 0.03960887499581389 1107 28-29 -0.015442318894677953 1107 30-31 -0.03298408739671288 1107 32-33 -0.018002621380695416 1107 34-35 0.08891768290534685 1107 36-37 0.22961957988930237 1107 38-39 0.17635456564593 1107 40-41 0.13679155098627405 1107 42-43 -0.06426624758916688 1107 44-45 -0.13724986662802507 1107 46-47 0.1511661025252522 1107 48-49 0.06165141323862855 1107 50-51 0.0780449955754392 1107 52-53 0.07556661895884531 1107 54-55 0.16358695830479064 1107 56-57 -0.2167159541393815 1107 58-59 -0.07309068933491858 1107 60-61 0.08638037466560888 1107 62-63 0.1012264499998281 1107 64-65 0.2521209307690242 1107 66-67 0.30582366274202855 1107 68-69 0.13822721940084648 1107 70-71 0.042173576991160644 1107 72-73 0.09750093361799728 1107 74-75 0.0527899756937984 1107 76-77 -0.09383158767802158 1107 78-79 -0.17771505088685302 1107 80-81 0.3689216132157398 1107 82-83 -0.006252987532242571 1107 84-85 0.1114627572225082 1107 86-87 0.2021709349534646 1107 88-89 0.2665985151730226 1107 90-91 0.01755214321004317 1107 92-93 0.13385778092610678 1107 94-95 0.4180381495647545 1107 96-97 0.4856783646850822 1107 98-99 0.4844489305458488 1107 100-101 0.19178431377967797 1108 1 -0.10309604393912508 1108 2 0.030375561155970843 1108 3 -0.012011815478992105 1108 4 -0.14133979017662313 1108 5 -0.1157722032825177 1108 6 0.0380801948107532 1108 7 0.00591912570795472 1108 8 0.041568813082683675 1108 9 -0.03582893409733856 1108 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5 50.0 6 102.0 7 247.0 8 546.0 9 1071.0 10 1807.0 11 2901.0 12 4287.0 13 6045.0 14 7861.0 15 10483.0 16 13407.0 17 17831.0 18 15261.0 19 9779.0 20 8673.0 21 8679.0 22 9126.0 23 9870.0 24 11607.0 25 13851.0 26 16742.0 27 20740.0 28 25754.0 29 32075.0 30 39539.0 31 49156.0 32 60807.0 33 78142.0 34 103274.0 35 140126.0 36 198851.0 37 258750.0 38 154150.0 39 8724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11410411802989 19.32061761492211 14.938083268152589 21.627194998895412 2 15.855073436969555 21.021663626223173 39.48331113623052 23.639951800576753 3 20.320531234261626 24.884445356363432 30.099121448956375 24.69590196041857 4 13.152721230800452 17.037582507604466 36.95572213132428 32.85397413027081 5 13.415512161566301 37.87316397690418 33.73992504825308 14.971398813276442 6 31.422255348071353 36.527291894686655 16.983264731490372 15.067188025751618 7 28.40437111992975 33.54051910593035 20.196099058226142 17.85901071591376 8 26.94213346882104 34.11343215878323 19.186282914149587 19.758151458246136 9 26.53635411350756 17.03754935403293 20.477335195580743 35.94876133687877 10-11 25.30865394945745 26.824020245669743 26.47914403552542 21.388181769347387 12-13 26.875859632604172 24.192891950500083 27.522269777005658 21.408978639890087 14-15 23.030400927550417 25.600600460566987 26.780966563941256 24.588032047941343 16-17 21.615718324802256 28.903253051286075 27.417722435089875 22.063306188821795 18-19 21.477300959530506 26.91001482456356 30.575569089404723 21.037115126501202 20-21 22.358679830461305 25.58401095838901 30.891532988197795 21.165776222951887 22-23 21.93820661684529 25.635640404186606 30.92560255132817 21.500550427639936 24-25 22.163567425286946 25.791485367760725 31.046057382968943 20.998889823983383 26-27 21.764800244727724 25.915882306857224 31.20207570890831 21.11724173950674 28-29 21.2654023114146 26.564107079103593 31.050060006946406 21.1204306025354 30-31 21.545909794945103 25.910712633661714 30.977136871383337 21.566240700009846 32-33 21.360813974092345 26.170199669859272 31.03934643326774 21.429639922780645 34-35 22.175604059834242 26.063262827818928 30.552696327607208 21.20843678473962 36-37 22.135380524079636 25.73505161254699 30.77684456840808 21.352723294965294 38-39 21.667874195177895 26.122796322646646 30.823875189057183 21.38545429311828 40-41 21.89574485149974 26.236470090109247 30.568913708878682 21.298871349512336 42-43 21.702103689710526 26.37966389758385 30.085639006054826 21.832593406650798 44-45 21.918991228986425 26.288307901671537 29.960479704907172 21.832221164434866 46-47 22.541867128638486 26.73370928229858 29.2724652635168 21.451958325546137 48-49 22.590522348194327 26.95940259577478 28.809466609765344 21.640608446265553 50-51 22.118412369541854 27.28537007269508 28.951198012123186 21.64501954563988 52-53 22.56995311154111 27.393581403087424 28.382271693419835 21.654193791951627 54-55 22.136506973674738 27.18985175228803 28.554220148542424 22.11942112549481 56-57 22.375792112874244 27.341160548181094 28.35390112909309 21.929146209851577 58-59 23.032224081627476 26.938604063318593 28.14356581536081 21.88560603969312 60-61 22.357715843166133 27.15565240066568 29.001641069489352 21.484990686678838 62-63 22.24213540257972 27.145540519114725 28.990738754535027 21.62158532377053 64-65 22.045048231319317 27.497037773241168 28.63842776643625 21.81948622900326 66-67 22.161962126417873 27.425928951780232 28.22895752521262 22.183151396589274 68-69 22.07559170852631 27.305382966777774 28.60927745249638 22.00974787219954 70-71 22.36914160714206 27.37808708523645 28.2333538753542 22.01941743226729 72-73 22.442429268325437 27.123218581699497 28.47568452384283 21.95866762613223 74-75 22.069934703187318 28.009023591985017 28.05673805431689 21.864303650510777 76-77 22.30888607028277 27.430314108781616 28.01447437140939 22.246325449526225 78-79 22.414096692795418 27.44473940636551 28.047595811640203 22.09356808919887 80-81 22.430807810811327 27.631138639550745 27.834157358533222 22.103896191104706 82-83 22.350943780546448 27.618931793449775 27.983819732450332 22.04630469355345 84-85 22.200381566901424 27.764550688931948 27.72910988365716 22.30595786050947 86-87 22.168448799871967 27.76691988216023 27.675596669239482 22.38903464872832 88-89 22.187828773102723 27.882570641914327 27.294976160452467 22.634624424530482 90-91 22.181215540520878 27.589707505292278 27.58202186155245 22.647055092634393 92-93 22.361023031641462 27.432460662672625 27.801921914752338 22.40459439093358 94-95 22.118759964993355 27.306487942777718 27.821627271757354 22.753124820471577 96-97 22.00347377915986 27.177770797723245 28.17939204388183 22.639363379235068 98-99 21.949869245203484 27.519538609037564 27.498946127531624 23.03164601822733 100-101 21.49650763403631 27.74016334640398 27.572884929684495 23.190444089875218 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 360.0 1 281.5 2 253.0 3 328.0 4 296.0 5 284.0 6 521.0 7 989.0 8 1559.0 9 1976.5 10 1964.0 11 1807.5 12 1810.5 13 1945.5 14 2171.5 15 2333.0 16 2538.5 17 2730.0 18 2907.0 19 3164.5 20 3458.0 21 3849.0 22 4324.5 23 4971.5 24 5868.0 25 7000.0 26 8628.0 27 10650.0 28 12426.5 29 14626.5 30 17369.5 31 20039.5 32 22837.5 33 25470.5 34 28028.5 35 29850.0 36 32082.0 37 34732.5 38 36185.0 39 37057.0 40 38211.0 41 40295.0 42 41924.5 43 43252.5 44 44901.0 45 46499.0 46 47198.0 47 50566.5 48 59798.0 49 59167.0 50 50112.0 51 45401.5 52 42572.0 53 41525.5 54 40075.5 55 37448.5 56 34387.5 57 31092.5 58 28519.5 59 25314.0 60 21586.5 61 18254.0 62 14991.0 63 11850.0 64 8824.5 65 6662.5 66 5122.5 67 4068.5 68 2981.5 69 1909.0 70 1424.0 71 1093.5 72 868.5 73 753.5 74 613.0 75 510.5 76 373.5 77 237.5 78 152.5 79 91.5 80 69.0 81 60.0 82 38.5 83 19.5 84 12.0 85 10.5 86 6.0 87 2.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0357371210205149 2 0.0058194059281840545 3 0.005073328245083534 4 8.952932197206238E-4 5 0.0010445087563407277 6 0.0 7 0.0 8 0.0 9 0.0020144097443714035 10-11 5.222543781703638E-4 12-13 0.0013056359454259095 14-15 0.002872399079937001 16-17 0.0035438689947274687 18-19 0.0 20-21 0.0 22-23 0.0016040670186661175 24-25 0.0011191165246507797 26-27 0.0058194059281840545 28-29 0.006080533117269236 30-31 0.0014175475978909877 32-33 4.1034272570528586E-4 34-35 0.010482391447562304 36-37 0.012310281771158577 38-39 0.010445087563407276 40-41 0.0019024980919063253 42-43 5.595582623253899E-4 44-45 0.0014175475978909877 46-47 1.1191165246507797E-4 48-49 8.206854514105717E-4 50-51 0.0013802437137359616 52-53 0.002573968006696793 54-55 0.003916907836277729 56-57 0.0014548514820460136 58-59 7.460776831005198E-4 60-61 0.00436455444613804 62-63 0.006005925348959184 64-65 0.010072048721857017 66-67 0.003133526269022183 68-69 0.0037676922996576247 70-71 7.460776831005199E-5 72-73 0.0010072048721857017 74-75 0.00585670981233908 76-77 0.003208134037332235 78-79 0.004252642793672963 80-81 0.0054090632024787685 82-83 0.005110632129238561 84-85 0.005968621464804158 86-87 0.003618476763037521 88-89 0.009922833185236913 90-91 0.01357861383242946 92-93 9.699009880306757E-4 94-95 0.0019398019760613514 96-97 0.0011937242929608318 98-99 0.003581172878882495 100-101 0.0046629855193782484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1340343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.219532918482656 #Duplication Level Percentage of deduplicated Percentage of total 1 81.15178865497342 48.86922808301918 2 11.350935082728816 13.67096017739895 3 3.2415854864649707 5.856202917307587 4 1.3668942604994745 3.292549356649324 5 0.7235526184059563 2.17860003611759 6 0.4498672768264683 1.6254478371479795 7 0.2922399270051159 1.2318986333065682 8 0.22493185420474757 1.083623295895851 9 0.15278482717306469 0.8280567836453747 >10 0.9313497094102046 10.279172484028594 >50 0.07188796053129345 2.9673113792675463 >100 0.038336332648558624 4.440034874740615 >500 0.0021091017798125712 0.8085251415024062 >1k 0.0013647129163493108 1.4002382371131117 >5k 3.721944317316302E-4 1.4681507628593404 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7627 0.5690334489007665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6849 0.510988605155546 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5175 0.386095201004519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4037 0.3011915606676798 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2476 0.1847288343356887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.15525876585321818 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1655 0.12347585655313603 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1400 0.10445087563407277 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.460776831005199E-5 0.0 0.0 0.0 0.0 12-13 7.460776831005199E-5 0.0 0.0 0.0 0.0 14-15 2.2382330493015594E-4 0.0 0.0 0.0 7.460776831005199E-5 16-17 2.9843107324020794E-4 0.0 0.0 0.0 1.1191165246507797E-4 18-19 2.9843107324020794E-4 0.0 0.0 0.0 1.4921553662010397E-4 20-21 2.9843107324020794E-4 0.0 0.0 3.730388415502599E-5 1.4921553662010397E-4 22-23 3.357349573952339E-4 0.0 0.0 7.460776831005199E-5 1.4921553662010397E-4 24-25 3.730388415502599E-4 0.0 0.0 2.2382330493015594E-4 1.4921553662010397E-4 26-27 3.730388415502599E-4 0.0 0.0 6.341660306354419E-4 1.4921553662010397E-4 28-29 3.730388415502599E-4 0.0 0.0 0.0014921553662010395 1.4921553662010397E-4 30-31 3.730388415502599E-4 0.0 0.0 0.003320045689797313 1.4921553662010397E-4 32-33 3.730388415502599E-4 0.0 0.0 0.006341660306354418 1.4921553662010397E-4 34-35 3.730388415502599E-4 0.0 0.0 0.009736313764461784 1.4921553662010397E-4 36-37 3.730388415502599E-4 0.0 0.0 0.01339209441165433 1.4921553662010397E-4 38-39 3.730388415502599E-4 0.0 0.0 0.017458217784552164 1.4921553662010397E-4 40-41 3.730388415502599E-4 0.0 0.0 0.02379987809090658 1.4921553662010397E-4 42-43 3.730388415502599E-4 0.0 0.0 0.030178842281416027 1.4921553662010397E-4 44-45 3.730388415502599E-4 0.0 0.0 0.037378491923336046 1.4921553662010397E-4 46-47 4.1034272570528586E-4 0.0 0.0 0.0472267173402629 1.4921553662010397E-4 48-49 4.476466098603119E-4 0.0 0.0 0.05543357185436862 1.4921553662010397E-4 50-51 4.476466098603119E-4 0.0 0.0 0.06793037304630233 1.4921553662010397E-4 52-53 4.476466098603119E-4 0.0 0.0 0.09441613079637078 1.4921553662010397E-4 54-55 4.476466098603119E-4 0.0 0.0 0.1284745770299095 1.4921553662010397E-4 56-57 4.476466098603119E-4 0.0 0.0 0.1668229699412762 1.4921553662010397E-4 58-59 4.476466098603119E-4 0.0 0.0 0.22490511757065168 1.4921553662010397E-4 60-61 4.476466098603119E-4 0.0 0.0 0.29361887218420957 1.4921553662010397E-4 62-63 4.476466098603119E-4 0.0 0.0 0.338756572011791 1.4921553662010397E-4 64-65 4.476466098603119E-4 0.0 0.0 0.37747800376470797 1.4921553662010397E-4 66-67 4.476466098603119E-4 0.0 0.0 0.4768182472695422 1.4921553662010397E-4 68-69 4.476466098603119E-4 0.0 0.0 0.5844772569409472 1.4921553662010397E-4 70-71 4.476466098603119E-4 0.0 0.0 0.6312563276713499 1.4921553662010397E-4 72-73 4.476466098603119E-4 0.0 0.0 0.7191442787405911 1.8651942077512997E-4 74-75 4.476466098603119E-4 0.0 0.0 0.8527667917838941 2.2382330493015594E-4 76-77 4.476466098603119E-4 0.0 0.0 0.9909776825782655 2.2382330493015594E-4 78-79 4.476466098603119E-4 0.0 0.0 1.1289274461835515 2.2382330493015594E-4 80-81 4.476466098603119E-4 0.0 0.0 1.2777326400779503 2.2382330493015594E-4 82-83 5.595582623253899E-4 0.0 0.0 1.4128846123716094 2.2382330493015594E-4 84-85 5.968621464804159E-4 0.0 0.0 1.5656440179864408 2.2382330493015594E-4 86-87 5.968621464804159E-4 0.0 0.0 1.824197239064926 2.2382330493015594E-4 88-89 5.968621464804159E-4 0.0 0.0 2.2067485710747174 2.2382330493015594E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6690 0.0 24.220106 1 TACATGG 6845 0.0 23.595198 2 TACCTGG 1230 0.0 22.785843 2 GTACCTG 1235 0.0 22.700367 1 ACATGGG 6770 0.0 22.452427 3 GAGTACT 7815 0.0 22.306477 12-13 GTACTTT 8180 0.0 21.164782 14-15 CATGGGG 2860 0.0 20.925415 4 GGTATCA 11520 0.0 20.417404 1 AGTACTT 7995 0.0 19.962488 12-13 GTATCAA 14395 0.0 19.772564 1 AGAGTAC 12300 0.0 18.692114 10-11 ACTTTTT 9360 0.0 18.67418 16-17 CATGGGA 3885 0.0 18.338753 4 GTATAGG 340 8.8766683E-10 18.168213 1 GTATAGA 450 0.0 17.950817 1 TACTTTT 9055 0.0 17.572191 14-15 TAAGGTG 490 0.0 17.448013 5 TCAACGC 16755 0.0 16.895514 4 ATCAACG 16825 0.0 16.854708 3 >>END_MODULE