FastQCFastQC Report
Thu 26 May 2016
SRR936664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326832
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54090.40766276363548665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.3330489466639333No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40390.30440929974555936No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27910.2103506698662679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.17990220314252292No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17430.13136553836506806No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16070.12111555946796579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG1703.623427E-927.955311
GTACATG63950.026.0100211
TACATGG66050.024.4635412
CGTACAC1401.7712458E-523.76473
ACATGGG66650.023.3903163
TACCTGG11100.022.6916522
GTACCGT1050.002189953622.6304871
GAGTACT50250.021.8501612-13
ACGCTCT1100.00286351921.6091659
GTACGGG1100.00286916621.601831
GTATCAA107200.021.146421
GTACCTG12750.020.500561
CATGGGA38450.020.3962334
GTACTTT53750.020.3354814-15
GTATTAG3750.020.2769171
GGTATCA83500.020.261741
GTGTAGG6150.020.0914571
CCGTGCG1200.004740643319.8084019
TAGACTG5600.019.521745
CGCGGAA1352.3086614E-819.34704682-83
Produced by FastQC (version 0.11.5)