##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936664_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1326832 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.499819118019463 31.0 31.0 34.0 30.0 34.0 2 31.557204680019776 33.0 31.0 34.0 30.0 34.0 3 31.51910565919423 33.0 31.0 34.0 28.0 34.0 4 35.170754850651775 37.0 35.0 37.0 33.0 37.0 5 35.20608486982527 37.0 35.0 37.0 33.0 37.0 6 35.273793517189816 37.0 35.0 37.0 33.0 37.0 7 35.14991649281899 37.0 35.0 37.0 32.0 37.0 8 35.13991974869464 37.0 35.0 37.0 32.0 37.0 9 36.65693923571334 39.0 37.0 39.0 32.0 39.0 10-11 36.536056938632775 39.0 37.0 39.0 32.0 39.0 12-13 36.44846295537039 39.0 37.0 39.0 32.0 39.0 14-15 37.471445518347466 40.0 37.0 41.0 32.0 41.0 16-17 37.397297095638336 40.0 37.0 41.0 32.0 41.0 18-19 37.29393736358484 40.0 37.0 41.0 32.0 41.0 20-21 37.286331653140714 40.0 37.0 41.0 31.5 41.0 22-23 37.27509096856271 39.5 37.0 41.0 31.5 41.0 24-25 37.39007877410252 40.0 37.0 41.0 32.0 41.0 26-27 37.31499654816887 40.0 37.0 41.0 31.0 41.0 28-29 37.20985625911946 39.5 36.5 41.0 31.0 41.0 30-31 37.00885454978475 39.0 36.0 41.0 31.0 41.0 32-33 36.83750693380925 39.0 36.0 41.0 30.0 41.0 34-35 36.63833326299034 39.0 35.5 41.0 30.0 41.0 36-37 36.430576742194944 39.0 35.0 41.0 30.0 41.0 38-39 36.24160406140341 39.0 35.0 40.0 30.0 41.0 40-41 36.009846009140574 39.0 35.0 40.0 29.5 41.0 42-43 35.73284522833335 38.0 35.0 40.0 28.0 41.0 44-45 35.56287570694707 38.0 35.0 40.0 27.0 41.0 46-47 35.38584462840812 39.0 35.0 40.0 26.5 41.0 48-49 35.14027171488176 38.5 35.0 40.0 26.0 41.0 50-51 34.15094299805853 37.5 33.5 39.5 24.0 40.5 52-53 34.255260650933955 38.0 33.5 39.5 24.0 40.5 54-55 34.69620193061367 38.0 34.0 40.0 24.5 41.0 56-57 34.539890882945244 38.0 34.0 40.0 24.0 41.0 58-59 34.323669839135626 38.0 34.0 40.0 23.5 41.0 60-61 34.0351431077936 38.0 33.5 40.0 23.0 41.0 62-63 33.70622656071002 37.0 33.0 40.0 22.0 41.0 64-65 33.349965180218746 36.5 33.0 39.5 22.0 41.0 66-67 32.98458508688364 36.0 32.0 39.0 20.5 41.0 68-69 32.55064507036309 35.5 32.0 39.0 19.5 40.5 70-71 32.14534809229805 35.0 31.5 38.5 18.0 40.0 72-73 31.736796369095714 35.0 31.0 37.5 16.0 40.0 74-75 31.32064647219844 35.0 31.0 37.0 12.5 39.0 76-77 30.863417523846273 35.0 30.5 36.5 9.0 39.0 78-79 30.397480615481086 34.5 30.0 36.0 7.0 38.5 80-81 29.9421366081011 34.0 30.0 35.5 4.5 37.5 82-83 29.54159908714894 34.0 29.0 35.0 2.0 37.0 84-85 29.17681477383723 34.0 29.0 35.0 2.0 36.5 86-87 28.837271410397097 34.0 29.0 35.0 2.0 36.0 88-89 28.48154853063538 34.0 29.0 35.0 2.0 36.0 90-91 28.120254862710578 34.0 28.0 35.0 2.0 35.5 92-93 27.686946048934608 34.0 27.0 35.0 2.0 35.0 94-95 27.206721725131743 33.5 26.0 35.0 2.0 35.0 96-97 26.62938412700327 33.0 25.0 35.0 2.0 35.0 98-99 25.908284545443582 33.0 24.0 35.0 2.0 35.0 100-101 23.29256077634546 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3402894668016607 1101 2 0.28084785289857095 1101 3 0.32853493029752556 1101 4 0.42848641482668626 1101 5 0.3788525334062456 1101 6 0.38815173740947984 1101 7 0.43567045998785403 1101 8 0.4464743039581478 1101 9 0.5486464923342496 1101 10-11 0.5524566965980711 1101 12-13 0.564169152547116 1101 14-15 0.7292363523921281 1101 16-17 0.7879843697868836 1101 18-19 0.9142633960373914 1101 20-21 0.6525993153221137 1101 22-23 0.6973874787663519 1101 24-25 0.6785240091231302 1101 26-27 0.5078663506451875 1101 28-29 0.49963923649539055 1101 30-31 0.3365398861297848 1101 32-33 0.2587283913150813 1101 34-35 0.3797956838562939 1101 36-37 0.2706110599948559 1101 38-39 0.41937375435096413 1101 40-41 0.36579795580155405 1101 42-43 0.11288989800935667 1101 44-45 0.13100471587485885 1101 46-47 0.3595464510605595 1101 48-49 0.285933947732552 1101 50-51 0.14217911882131062 1101 52-53 0.29832757679750443 1101 54-55 0.24476957087023266 1101 56-57 0.35190167083084134 1101 58-59 0.35560541940180457 1101 60-61 0.2543935729496596 1101 62-63 0.1616424135252572 1101 64-65 0.23325552305815478 1101 66-67 0.1968575583213834 1101 68-69 0.2820468122857065 1101 70-71 0.318148744120883 1101 72-73 0.23081090740904742 1101 74-75 0.1628483496172528 1101 76-77 0.22552811806395212 1101 78-79 0.2509193838285242 1101 80-81 0.17737426265307477 1101 82-83 0.3561242464828034 1101 84-85 0.4228677235412661 1101 86-87 0.3517676663685343 1101 88-89 0.5390163480720851 1101 90-91 0.3120511621079096 1101 92-93 0.21820798125623853 1101 94-95 0.5118316776346674 1101 96-97 0.44301033561052705 1101 98-99 0.1332895648054624 1101 100-101 0.8145770749824557 1102 1 0.29251280739137897 1102 2 0.4448296573195023 1102 3 0.5193169812794771 1102 4 0.4245102282517763 1102 5 0.42736263382523987 1102 6 0.4462652335042918 1102 7 0.5521783444786976 1102 8 0.46211220257163177 1102 9 0.4411583717378207 1102 10-11 0.5112997324196371 1102 12-13 0.5423828335626908 1102 14-15 0.6839994275386161 1102 16-17 0.6007376605182202 1102 18-19 0.7012175091589654 1102 20-21 0.811336236274343 1102 22-23 0.5830604108918536 1102 24-25 0.746654436781661 1102 26-27 0.5226334831249204 1102 28-29 0.4718316789319701 1102 30-31 0.4683977546253715 1102 32-33 0.6359160351748514 1102 34-35 0.3582933781986597 1102 36-37 0.40576395123491693 1102 38-39 0.4358036132025873 1102 40-41 0.7405660245889578 1102 42-43 0.5301928637639008 1102 44-45 0.6381713708376822 1102 46-47 0.7575747431573419 1102 48-49 0.9101367207637665 1102 50-51 0.8097742905947527 1102 52-53 0.5814568730842637 1102 54-55 0.8234951000791284 1102 56-57 0.7450393158609927 1102 58-59 0.6752725620248796 1102 60-61 0.7204649039890327 1102 62-63 0.7206586662659049 1102 64-65 0.7414037405624256 1102 66-67 0.733699704650661 1102 68-69 0.7080824075823244 1102 70-71 0.6042640816207836 1102 72-73 0.7964499764071213 1102 74-75 0.6684615141031038 1102 76-77 0.8492502531575461 1102 78-79 0.6514398148421527 1102 80-81 0.8324073065112891 1102 82-83 0.5380870594631517 1102 84-85 0.5413629210631719 1102 86-87 0.3605728597550808 1102 88-89 0.5657104797637587 1102 90-91 0.6801417966904317 1102 92-93 0.6332466429604722 1102 94-95 0.9268578552366264 1102 96-97 0.8690904055214972 1102 98-99 0.8670169276168807 1102 100-101 1.2336246940300732 1103 1 0.30386508396441414 1103 2 0.36488081187655297 1103 3 0.3875813058570934 1103 4 0.34291513023808307 1103 5 0.2766024745041804 1103 6 0.3294541929711414 1103 7 0.5218497447858823 1103 8 0.21924813311598257 1103 9 0.2933138920043987 1103 10-11 0.253741906943894 1103 12-13 0.3328473247505812 1103 14-15 0.4979933879166154 1103 16-17 0.7505645420426958 1103 18-19 0.7697229630417581 1103 20-21 0.6645098038165784 1103 22-23 0.5929101037041775 1103 24-25 0.32592642803462013 1103 26-27 0.4396413971123678 1103 28-29 0.4177620931146677 1103 30-31 0.4376207390316935 1103 32-33 0.395277286574121 1103 34-35 0.34419723887082654 1103 36-37 0.32301999085692046 1103 38-39 0.32875538083333566 1103 40-41 0.3836289199629235 1103 42-43 0.3608283257168807 1103 44-45 0.36928844057969457 1103 46-47 0.5653795553303027 1103 48-49 0.6428901180511488 1103 50-51 0.485617592341562 1103 52-53 0.6920572883082698 1103 54-55 0.5371586059235085 1103 56-57 0.6681229724355973 1103 58-59 0.5082874267842001 1103 60-61 0.49895216741547443 1103 62-63 0.48262987392998014 1103 64-65 0.43090729404692496 1103 66-67 0.5219971234932359 1103 68-69 0.6099338315794114 1103 70-71 0.565104161801969 1103 72-73 0.7142504280771256 1103 74-75 0.6157968888460275 1103 76-77 0.605029872036571 1103 78-79 0.5438110498407234 1103 80-81 0.8075579716506915 1103 82-83 0.7111477745856334 1103 84-85 0.7376154918074498 1103 86-87 0.560220440001352 1103 88-89 0.4935218652322213 1103 90-91 0.6465864248111224 1103 92-93 0.7826010281946374 1103 94-95 0.7225819590556455 1103 96-97 0.5718127911721886 1103 98-99 0.6101315632870978 1103 100-101 1.1702485258057713 1104 1 0.2308447238863991 1104 2 0.2779460407865102 1104 3 0.4195101865037465 1104 4 0.4539647847029826 1104 5 0.28810434028098086 1104 6 0.2145785686968793 1104 7 0.35052450775894783 1104 8 0.47458687588679993 1104 9 0.6692453712102875 1104 10-11 0.5644800584156542 1104 12-13 0.5603403351335388 1104 14-15 0.5639128043963311 1104 16-17 0.6243653154100031 1104 18-19 0.6994265807322577 1104 20-21 0.632064804774835 1104 22-23 0.5162698688755967 1104 24-25 0.4648718413802797 1104 26-27 0.10657628093817095 1104 28-29 0.11029363094315414 1104 30-31 0.12227697951961858 1104 32-33 0.17983326269668254 1104 34-35 0.2699508774174362 1104 36-37 0.2949096817354757 1104 38-39 0.2473633337774217 1104 40-41 0.1735782452394048 1104 42-43 0.45236694335100935 1104 44-45 0.4146448546806809 1104 46-47 0.4086405639771371 1104 48-49 0.6306332749905934 1104 50-51 0.5154400757975921 1104 52-53 0.4045356571345806 1104 54-55 0.4650249830471722 1104 56-57 0.4155251177970811 1104 58-59 0.4838068567922065 1104 60-61 0.3459827779923401 1104 62-63 0.5541550760088256 1104 64-65 0.5023141235791897 1104 66-67 0.6089371790719724 1104 68-69 0.6589946722499604 1104 70-71 0.5978059869939045 1104 72-73 0.703011729836934 1104 74-75 0.6658878525112932 1104 76-77 0.7877526942635527 1104 78-79 0.6486190185678211 1104 80-81 0.9146717389415393 1104 82-83 0.8990885009421277 1104 84-85 0.7387146654543422 1104 86-87 0.7531683955383706 1104 88-89 0.8430375946889477 1104 90-91 0.9114612035671925 1104 92-93 0.9723522203752886 1104 94-95 1.185086045726898 1104 96-97 1.1114154539908085 1104 98-99 0.7429603571272168 1104 100-101 1.1636608822085996 1105 1 0.35565044990458006 1105 2 0.36377908990950303 1105 3 0.3816297165991429 1105 4 0.39293929275204675 1105 5 0.3386609729110788 1105 6 0.33059323013559805 1105 7 0.4782474908001788 1105 8 0.4278604067863796 1105 9 0.40013289334626734 1105 10-11 0.5429599946915005 1105 12-13 0.5273389365897643 1105 14-15 0.7363746915987761 1105 16-17 0.5757136550493698 1105 18-19 0.6173084850407378 1105 20-21 0.5606141341528783 1105 22-23 0.4730538743568431 1105 24-25 0.4511362798378684 1105 26-27 0.520109957765456 1105 28-29 0.570073861958484 1105 30-31 0.31214303061338455 1105 32-33 0.3714057203111949 1105 34-35 0.20476179933474015 1105 36-37 0.36996047718108116 1105 38-39 0.15249926261878244 1105 40-41 0.31655245114808395 1105 42-43 0.18973999288601817 1105 44-45 0.32478803565438596 1105 46-47 0.5000271594729568 1105 48-49 0.3948794614219011 1105 50-51 0.5146648873222617 1105 52-53 0.5518199414853129 1105 54-55 0.446495422501215 1105 56-57 0.5210260947939673 1105 58-59 0.5685267169530874 1105 60-61 0.47653146036268623 1105 62-63 0.7380136071640067 1105 64-65 0.7511736580540926 1105 66-67 0.5672650761672315 1105 68-69 0.6749175089514736 1105 70-71 0.6592167842401508 1105 72-73 0.7361420721329957 1105 74-75 0.7693993571170203 1105 76-77 0.6542079771043454 1105 78-79 0.7240015199087644 1105 80-81 0.7114033581622472 1105 82-83 0.8014662542536577 1105 84-85 0.955026393460848 1105 86-87 0.8590867230234522 1105 88-89 0.7959513801494325 1105 90-91 0.9143643454457617 1105 92-93 0.9621765364202375 1105 94-95 1.2245903294825027 1105 96-97 1.2494619485137513 1105 98-99 1.606010265989159 1105 100-101 1.7250857945993339 1106 1 0.4409634922490646 1106 2 0.17201937153938118 1106 3 0.23702182071746947 1106 4 0.3310384429761726 1106 5 0.4541273664882439 1106 6 0.3251947333937295 1106 7 0.42448615971670733 1106 8 0.47582533723336695 1106 9 0.6186382515462725 1106 10-11 0.5894647486514444 1106 12-13 0.6561933404744522 1106 14-15 0.4857593752103284 1106 16-17 0.3522834449873429 1106 18-19 0.6030136926870711 1106 20-21 0.4782982406560947 1106 22-23 -0.023554026588293198 1106 24-25 0.015140577031154123 1106 26-27 0.06710565677457225 1106 28-29 0.14270566920590255 1106 30-31 0.2198037987662218 1106 32-33 0.13285642812019915 1106 34-35 0.2049883893574389 1106 36-37 0.1615757180395292 1106 38-39 0.13374355830990936 1106 40-41 0.061015869518080024 1106 42-43 -0.06615103123759525 1106 44-45 -0.0806127396463765 1106 46-47 0.12927398183270356 1106 48-49 -0.027273363210504442 1106 50-51 -0.08379375198144601 1106 52-53 0.10926241103806689 1106 54-55 0.25462114325368645 1106 56-57 0.299995544804716 1106 58-59 0.326284471543012 1106 60-61 0.33844699418472857 1106 62-63 0.5875814615927837 1106 64-65 0.24492660910431852 1106 66-67 0.25618693383408697 1106 68-69 0.4170772620907215 1106 70-71 0.17938751581367995 1106 72-73 0.3757656204483659 1106 74-75 0.4902912947739644 1106 76-77 0.25539919925159893 1106 78-79 0.317819072323001 1106 80-81 0.5337909587511298 1106 82-83 0.7392964079424509 1106 84-85 0.8637696880628987 1106 86-87 0.9651551160782361 1106 88-89 0.8901860354266802 1106 90-91 1.1141399495908786 1106 92-93 1.1458665697082928 1106 94-95 1.2506583090210448 1106 96-97 1.1975983616147907 1106 98-99 1.3288455190083575 1106 100-101 2.018481265077437 1107 1 0.2890366369025763 1107 2 0.25873243138750013 1107 3 0.2382966432018634 1107 4 0.2880485958634793 1107 5 0.2151826819039968 1107 6 0.3215790809496326 1107 7 0.40294531513539056 1107 8 0.4706003927498301 1107 9 0.34356994116583905 1107 10-11 0.5380692978029415 1107 12-13 0.4432509222412975 1107 14-15 0.3174275192698346 1107 16-17 0.40083019304769607 1107 18-19 0.6423897150581439 1107 20-21 0.41608210569854265 1107 22-23 0.2777335898135149 1107 24-25 0.41957960944545647 1107 26-27 0.29692279514078734 1107 28-29 0.3944840237253473 1107 30-31 0.2711231714987221 1107 32-33 0.315965258664761 1107 34-35 0.35883044844705836 1107 36-37 0.21343174991466185 1107 38-39 0.3555197195863755 1107 40-41 0.2674882209487066 1107 42-43 0.37870375779984755 1107 44-45 0.3122822953998181 1107 46-47 0.38850159476186974 1107 48-49 0.39437280272660047 1107 50-51 0.17448505489436883 1107 52-53 0.2659266712297992 1107 54-55 0.46435822825100104 1107 56-57 0.3985336112782534 1107 58-59 0.6269534106531083 1107 60-61 0.5220742068470514 1107 62-63 0.3975255338966903 1107 64-65 0.3592167294585238 1107 66-67 0.40762556415384665 1107 68-69 0.5794087707677349 1107 70-71 0.6022216883670737 1107 72-73 0.6116539295494086 1107 74-75 0.670411374827335 1107 76-77 0.5483773947609301 1107 78-79 0.5227614083095489 1107 80-81 0.5510111146541483 1107 82-83 0.5746135600164344 1107 84-85 0.5975501992712857 1107 86-87 0.826477961445466 1107 88-89 0.6795855894672727 1107 90-91 0.5589776039175121 1107 92-93 0.6330587160707104 1107 94-95 0.7316332969125163 1107 96-97 1.0939034325753383 1107 98-99 1.3020772168525845 1107 100-101 1.7630767488245915 1108 1 0.17317539895229928 1108 2 0.007865697073278 1108 3 0.2413513531629654 1108 4 0.1979399369762831 1108 5 0.009100743801511157 1108 6 0.1636600888982187 1108 7 0.30068757794614953 1108 8 0.35085364828847077 1108 9 -0.11568720410728872 1108 10-11 0.1284390339774646 1108 12-13 0.09989835163415961 1108 14-15 0.4551188350148081 1108 16-17 0.4536661054441069 1108 18-19 0.5580976890138416 1108 20-21 0.4774352682217895 1108 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94-95 -4.435691131873011 2316 96-97 -5.770530743009022 2316 98-99 -7.282610422828746 2316 100-101 -7.93480140961676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3159.0 3 1184.0 4 1096.0 5 1220.0 6 1385.0 7 1607.0 8 1947.0 9 2511.0 10 3106.0 11 3961.0 12 5152.0 13 6580.0 14 7377.0 15 8912.0 16 11158.0 17 14286.0 18 11270.0 19 8486.0 20 8266.0 21 8753.0 22 9673.0 23 11110.0 24 12522.0 25 14270.0 26 17033.0 27 19786.0 28 23682.0 29 29248.0 30 35215.0 31 43763.0 32 54881.0 33 70347.0 34 93711.0 35 129282.0 36 181978.0 37 243132.0 38 195742.0 39 30031.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.876970352502454 18.549485660495197 14.215502142892703 22.358041844109646 2 15.548332960140772 20.165893517230565 40.09852283599204 24.187250686636617 3 19.963621643991125 24.57182313153874 29.603885986342664 25.86066923812747 4 12.620256275338438 16.2391390547755 37.45452462115787 33.68608004872819 5 13.551504734334479 37.58066461612689 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28.39117468170112 22.167492325629947 52-53 22.46519054242422 27.56297808951482 27.873982396395018 22.097848971665936 54-55 21.928773710222316 27.312404965980885 28.125886748654217 22.632934575142585 56-57 22.26090004126555 27.54398361437247 27.802166535709777 22.392949808652197 58-59 22.664261867821732 27.149970436198906 27.807986409319025 22.37778128666034 60-61 22.22741449458002 27.220864694239644 28.601711841948568 21.950008969231767 62-63 22.100312593622636 27.326712151959313 28.4817446454917 22.091230608926345 64-65 21.767942268196634 27.751625120117573 28.150245887739526 22.330186723946262 66-67 21.867353718017274 27.63226171863077 27.796751579611396 22.70363298374056 68-69 21.832339133970642 27.49269942744819 28.118387418557493 22.556574020023675 70-71 22.098690480631934 27.42483802004018 27.907584087694726 22.568887411633163 72-73 22.07549658889601 27.316761524254645 28.17537974444989 22.432362142399455 74-75 21.776426651148643 28.06099969886091 27.8114575320389 22.351116117951545 76-77 21.97445230850068 27.597447266282423 27.75274321626205 22.675357208954846 78-79 22.103528168395627 27.57524782964141 27.808556902349036 22.512667099613928 80-81 22.176635178691495 27.784243549889798 27.612494384326165 22.426626887092542 82-83 21.977007370514777 27.702214320008018 27.926291837413032 22.394486472064173 84-85 21.951343596422504 27.83044234087671 27.62656735957781 22.59164670312298 86-87 21.975210569969978 27.8753903613577 27.581227382405466 22.568171686266854 88-89 21.90683634746628 27.76365027264745 27.31957597386202 23.009937406024246 90-91 21.910256965891765 27.548771135100708 27.70083645346745 22.840135445540078 92-93 22.105720965063064 27.554052734982275 27.75730285247388 22.58292344748078 94-95 21.82723209456747 27.31188323491977 27.85600651877559 23.004878151737167 96-97 21.552043839085833 27.303662553611673 28.167592543737328 22.976701063565166 98-99 21.58483457053321 27.484457417432267 27.6065063621499 23.324201649884625 100-101 21.436236273210923 27.304055798561315 27.890649293377784 23.369058634849978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 157.0 1 143.0 2 176.5 3 255.5 4 248.0 5 250.5 6 404.5 7 707.5 8 1177.5 9 1512.0 10 1456.0 11 1368.5 12 1414.5 13 1549.0 14 1733.0 15 1828.0 16 1930.0 17 2040.5 18 2207.5 19 2409.0 20 2613.0 21 3028.5 22 3620.5 23 4300.5 24 5169.5 25 6372.0 26 8001.0 27 9869.0 28 11560.0 29 13654.5 30 16312.0 31 19268.0 32 22643.0 33 25084.5 34 27465.0 35 29798.0 36 32033.5 37 34262.5 38 35747.0 39 36792.5 40 37858.5 41 40071.0 42 42031.0 43 43440.0 44 45043.5 45 47345.0 46 48575.0 47 51334.5 48 59339.5 49 58610.0 50 50575.5 51 46246.0 52 43033.0 53 41974.5 54 41111.0 55 38426.5 56 35000.0 57 31501.0 58 28759.0 59 25481.0 60 21482.5 61 17974.0 62 14816.5 63 11542.5 64 8671.5 65 6713.0 66 4957.5 67 3845.0 68 2856.0 69 1880.0 70 1380.5 71 1026.5 72 790.5 73 686.0 74 560.0 75 437.5 76 335.5 77 222.5 78 130.0 79 82.0 80 56.5 81 39.0 82 28.0 83 19.0 84 14.5 85 8.5 86 6.0 87 6.0 88 6.0 89 6.5 90 5.0 91 2.0 92 0.5 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0794373364525426 2 0.03308632893991101 3 0.016505480724010275 4 0.02185657264823278 5 0.026529357145441173 6 0.03549808868040566 7 0.08403475345786053 8 0.06021862602047585 9 0.07770388413906207 10-11 0.06507982924741038 12-13 0.04190432549109458 14-15 0.04684089620991957 16-17 0.05562120901515791 18-19 0.05433996165302013 20-21 0.01948249665368336 22-23 0.015488019583489093 24-25 0.023891494929275146 26-27 0.02626557092382457 28-29 0.0071975954755387275 30-31 9.044099026854945E-4 32-33 0.010438397626828416 34-35 0.012096482448418489 36-37 0.00493657071882499 38-39 0.01066450010249979 40-41 0.005087305702605907 42-43 0.0025248109783303388 44-45 4.145212053975183E-4 46-47 3.391537135070604E-4 48-49 7.536749189045787E-4 50-51 9.797773945759525E-4 52-53 1.1305123783568681E-4 54-55 3.391537135070604E-4 56-57 0.004484365767482243 58-59 0.0030523834215635437 60-61 0.0055771943998938825 62-63 0.002223341010768507 64-65 5.275724432332052E-4 66-67 1.8841872972614467E-4 68-69 0.003956793324249038 70-71 0.003579955864796749 72-73 0.02125363271310912 74-75 0.01526191710781772 76-77 0.025059691053577245 78-79 0.020010069096916567 80-81 0.014169088475406079 82-83 0.004446682021537014 84-85 0.002901648437782628 86-87 0.001243563616192555 88-89 0.0020349222810423628 90-91 0.007499065443100559 92-93 0.01066450010249979 94-95 0.01526191710781772 96-97 0.012209533686254175 98-99 0.02374075994549423 100-101 0.020688376523930685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1326832.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.061399179593366 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52545767306981 52.04170828800091 2 10.625818902325527 13.401580148192375 3 2.9669323931405724 5.612967239481115 4 1.2174342596074017 3.0709243128006025 5 0.6802021637952055 2.14472500869563 6 0.4172102339174732 1.578591666172878 7 0.2759390213919271 1.218077054405587 8 0.19741251258541723 0.9959287407356398 9 0.15663979099760222 0.8890131948757083 >10 0.8385607807694164 9.837754773455076 >50 0.06380838698836273 2.771379173112374 >100 0.03170191862605728 3.7316114086507737 >500 0.001681144958050512 0.7523303820148185 >1k 0.0010807360444610435 1.5438107194902975 >5k 1.2008178271789372E-4 0.40959788991627105 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5409 0.40766276363548665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4419 0.3330489466639333 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4039 0.30440929974555936 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2791 0.2103506698662679 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2387 0.17990220314252292 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1743 0.13136553836506806 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1607 0.12111555946796579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 3.768374594522894E-5 0.0 0.0 0.0 0.0 20-21 7.536749189045788E-5 0.0 0.0 3.768374594522894E-5 0.0 22-23 7.536749189045788E-5 0.0 0.0 1.1305123783568681E-4 3.768374594522894E-5 24-25 1.1305123783568681E-4 0.0 0.0 3.014699675618315E-4 7.536749189045788E-5 26-27 1.5073498378091575E-4 0.0 0.0 3.391537135070604E-4 7.536749189045788E-5 28-29 1.5073498378091575E-4 0.0 0.0 0.001205879870247326 7.536749189045788E-5 30-31 1.5073498378091575E-4 0.0 0.0 0.003052383421563544 7.536749189045788E-5 32-33 1.5073498378091575E-4 0.0 0.0 0.006556971794469835 7.536749189045788E-5 34-35 1.8841872972614467E-4 0.0 0.0 0.009345568994416777 7.536749189045788E-5 36-37 2.2610247567137363E-4 0.0 0.0 0.012586371145706465 7.536749189045788E-5 38-39 2.2610247567137363E-4 0.0 0.0 0.01624169450239367 7.536749189045788E-5 40-41 2.2610247567137363E-4 0.0 0.0 0.02272329880497305 7.536749189045788E-5 42-43 2.2610247567137363E-4 0.0 0.0 0.029657108058895175 7.536749189045788E-5 44-45 2.2610247567137363E-4 0.0 0.0 0.03726922473983142 7.536749189045788E-5 46-47 2.2610247567137363E-4 0.0 0.0 0.04785835735044075 7.536749189045788E-5 48-49 2.2610247567137363E-4 0.0 0.0 0.05675172139351478 7.536749189045788E-5 50-51 2.2610247567137363E-4 0.0 0.0 0.06948882752300216 7.536749189045788E-5 52-53 2.2610247567137363E-4 0.0 0.0 0.09575439844682673 7.536749189045788E-5 54-55 2.2610247567137363E-4 0.0 0.0 0.13102638465156102 7.536749189045788E-5 56-57 2.2610247567137363E-4 0.0 0.0 0.16897391681840657 7.536749189045788E-5 58-59 2.2610247567137363E-4 0.0 0.0 0.22402986964438604 7.536749189045788E-5 60-61 2.2610247567137363E-4 0.0 0.0 0.29348101342144295 7.536749189045788E-5 62-63 2.2610247567137363E-4 0.0 0.0 0.33855077357193675 7.536749189045788E-5 64-65 2.2610247567137363E-4 0.0 0.0 0.3778172368468653 7.536749189045788E-5 66-67 2.2610247567137363E-4 0.0 0.0 0.479487983407093 7.536749189045788E-5 68-69 2.2610247567137363E-4 0.0 0.0 0.5885447441725855 7.536749189045788E-5 70-71 2.2610247567137363E-4 0.0 0.0 0.6371944601878761 7.536749189045788E-5 72-73 2.2610247567137363E-4 0.0 0.0 0.7300848939428655 7.536749189045788E-5 74-75 2.2610247567137363E-4 0.0 0.0 0.8635984058268116 7.536749189045788E-5 76-77 2.2610247567137363E-4 0.0 0.0 1.0028775308403777 7.536749189045788E-5 78-79 3.014699675618315E-4 0.0 0.0 1.1418928696323274 7.536749189045788E-5 80-81 3.014699675618315E-4 0.0 0.0 1.2986572527644795 7.536749189045788E-5 82-83 3.014699675618315E-4 0.0 0.0 1.445548494458982 7.536749189045788E-5 84-85 3.014699675618315E-4 0.0 0.0 1.6098119430342348 7.536749189045788E-5 86-87 3.014699675618315E-4 0.0 0.0 1.872882173477878 7.536749189045788E-5 88-89 3.014699675618315E-4 0.0 0.0 2.261326226681298 7.536749189045788E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 170 3.623427E-9 27.95531 1 GTACATG 6395 0.0 26.010021 1 TACATGG 6605 0.0 24.463541 2 CGTACAC 140 1.7712458E-5 23.7647 3 ACATGGG 6665 0.0 23.390316 3 TACCTGG 1110 0.0 22.691652 2 GTACCGT 105 0.0021899536 22.630487 1 GAGTACT 5025 0.0 21.85016 12-13 ACGCTCT 110 0.002863519 21.609165 9 GTACGGG 110 0.002869166 21.60183 1 GTATCAA 10720 0.0 21.14642 1 GTACCTG 1275 0.0 20.50056 1 CATGGGA 3845 0.0 20.396233 4 GTACTTT 5375 0.0 20.33548 14-15 GTATTAG 375 0.0 20.276917 1 GGTATCA 8350 0.0 20.26174 1 GTGTAGG 615 0.0 20.091457 1 CCGTGCG 120 0.0047406433 19.808401 9 TAGACTG 560 0.0 19.52174 5 CGCGGAA 135 2.3086614E-8 19.347046 82-83 >>END_MODULE