FastQCFastQC Report
Thu 26 May 2016
SRR936664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326832
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75180.5666128040324623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68970.5198095915684879No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51760.3901021380250099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40090.3021482749888456No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24050.1812588179965512No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20300.15299600853762946No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14590.10996117066817802No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14230.10724794096012155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11450.026.9635562
GTACCTG12900.024.6766031
GTACATG66750.023.6312731
GAGTACT77100.022.94348512-13
TACATGG69350.022.4644972
ACATGGG67250.021.8215923
TAAGGTG5050.021.6283765
GTACTTT82900.021.39555414-15
GTATCAA143250.021.260021
GGTATCA111350.021.03571
AGTACTT79900.020.59415212-13
CATGGGG28750.020.3167724
ACTTTTT91500.019.59220516-17
AGAGTAC122250.018.97593310-11
GTAAGGT5050.018.8072834
TACTTTT88550.018.555614-15
CAACGCA165350.018.3520325
TATCAAC166350.018.3308072
TCAACGC165650.018.3187944
ATCAACG166500.018.2551733
Produced by FastQC (version 0.11.5)