##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936664_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1326832 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.947408564158838 31.0 31.0 34.0 30.0 34.0 2 32.095969195798716 33.0 31.0 34.0 30.0 34.0 3 32.16920981706802 33.0 31.0 34.0 30.0 34.0 4 35.866556579883515 37.0 35.0 37.0 35.0 37.0 5 35.7168066492216 37.0 35.0 37.0 33.0 37.0 6 35.814076687930346 37.0 35.0 37.0 35.0 37.0 7 35.762529845526785 37.0 35.0 37.0 33.0 37.0 8 35.73683706754133 37.0 35.0 37.0 33.0 37.0 9 37.36732005257636 39.0 37.0 39.0 34.0 39.0 10-11 37.26265457872587 39.0 37.0 39.0 33.5 39.0 12-13 37.15781425229419 39.0 37.0 39.0 33.0 39.0 14-15 38.26949116391525 40.0 38.0 41.0 33.0 41.0 16-17 38.18511160418199 40.0 37.5 41.0 33.0 41.0 18-19 38.352867959168904 40.0 38.0 41.0 33.5 41.0 20-21 38.38201143777057 40.0 38.0 41.0 34.0 41.0 22-23 38.300553498860445 40.0 38.0 41.0 34.0 41.0 24-25 38.16430037864628 40.0 37.5 41.0 33.0 41.0 26-27 37.96677989376198 40.0 37.0 41.0 33.0 41.0 28-29 37.8789628227236 40.0 37.0 41.0 33.0 41.0 30-31 37.81936258697409 40.0 37.0 41.0 33.0 41.0 32-33 37.62220688075054 40.0 36.5 41.0 33.0 41.0 34-35 37.43330730642613 40.0 36.0 41.0 32.5 41.0 36-37 37.2067937010865 39.0 36.0 41.0 31.5 41.0 38-39 37.02225413616796 39.0 36.0 41.0 31.0 41.0 40-41 36.810845683553005 39.0 35.0 41.0 31.0 41.0 42-43 36.501208140895 39.0 35.0 41.0 30.0 41.0 44-45 36.18102706295899 39.0 35.0 40.0 30.0 41.0 46-47 35.723097196932244 39.0 35.0 40.0 28.5 41.0 48-49 35.2589898344327 38.0 35.0 40.0 27.0 41.0 50-51 34.93548844164144 38.0 34.0 40.0 26.0 41.0 52-53 34.57817719198813 38.0 34.0 40.0 24.0 41.0 54-55 34.37458510195714 38.0 34.0 40.0 24.0 41.0 56-57 34.0696180827716 38.0 33.5 40.0 23.0 41.0 58-59 33.699463458825235 37.0 33.0 40.0 22.5 41.0 60-61 33.279929561542076 37.0 32.5 39.5 21.5 41.0 62-63 32.96741034283164 36.0 32.0 39.0 20.5 40.5 64-65 32.54961140521181 36.0 31.5 39.0 19.5 40.0 66-67 32.13416770171355 35.0 31.0 38.5 18.0 40.0 68-69 31.778776438916154 35.0 31.0 38.0 18.0 40.0 70-71 31.405371215044557 35.0 31.0 37.5 14.0 40.0 72-73 30.95603550411808 34.5 30.0 37.0 11.5 39.0 74-75 30.458008248218313 34.0 30.0 36.5 7.5 39.0 76-77 28.995993464131104 32.5 28.0 35.0 7.0 37.0 78-79 29.751924508905425 34.0 29.0 35.0 6.5 37.5 80-81 29.521031298612034 34.0 29.0 35.0 2.0 37.0 82-83 29.20730582319389 34.0 29.0 35.0 2.0 37.0 84-85 28.833791316459056 34.0 29.0 35.0 2.0 36.0 86-87 28.458639074125436 34.0 29.0 35.0 2.0 36.0 88-89 27.990951378923633 33.0 27.5 35.0 2.0 35.0 90-91 27.509954915166354 33.0 26.5 35.0 2.0 35.0 92-93 26.871019842753263 32.5 25.5 34.5 2.0 35.0 94-95 26.339369264533865 32.0 25.0 34.0 2.0 35.0 96-97 25.635093214513972 32.0 23.5 34.0 2.0 35.0 98-99 24.767327740060534 32.0 17.5 34.0 2.0 35.0 100-101 21.590002728303205 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26861804931849775 1101 2 0.2271017830040165 1101 3 0.14089872513966384 1101 4 0.1978334789741254 1101 5 0.1700494983476446 1101 6 0.18873472931475987 1101 7 0.12536540058779622 1101 8 0.14352677588714613 1101 9 0.17054340690025782 1101 10-11 0.1508311816062573 1101 12-13 0.240598488887386 1101 14-15 0.3755796041325681 1101 16-17 0.43454945655835076 1101 18-19 0.20097491658729183 1101 20-21 0.12925113447578696 1101 22-23 0.058410207770393185 1101 24-25 0.08386444490314204 1101 26-27 0.1401320652028062 1101 28-29 0.059165375442120194 1101 30-31 0.1367706714338155 1101 32-33 0.2275351116729567 1101 34-35 0.43458263484223636 1101 36-37 0.26126194911967815 1101 38-39 0.20028971529185924 1101 40-41 0.3312379400254599 1101 42-43 0.2755195861983708 1101 44-45 0.3598725266189007 1101 46-47 0.3735253183857665 1101 48-49 0.5663078556420373 1101 50-51 0.6298055666577724 1101 52-53 0.6351706160849631 1101 54-55 0.5594974461830304 1101 56-57 0.5919128812230525 1101 58-59 0.42150422804506604 1101 60-61 0.5613510182006465 1101 62-63 0.5836787502247276 1101 64-65 0.5722317964992811 1101 66-67 0.38643999569306686 1101 68-69 0.41469958745823376 1101 70-71 0.2462589664956134 1101 72-73 0.5671888576524573 1101 74-75 0.41188891999347277 1101 76-77 0.07898150318652952 1101 78-79 0.2973041895306139 1101 80-81 0.19501520056991595 1101 82-83 0.2006052656466757 1101 84-85 0.19548201801368847 1101 86-87 0.32757305812044635 1101 88-89 0.276249794855044 1101 90-91 0.3202215897091847 1101 92-93 0.251376500160994 1101 94-95 0.1565062937768893 1101 96-97 0.38934652456242347 1101 98-99 0.03866873702386542 1101 100-101 0.515819540797235 1102 1 0.23193351478045088 1102 2 0.26898594614002036 1102 3 0.17112429726266498 1102 4 0.16476465255776418 1102 5 0.1541603571758543 1102 6 0.15603418391554413 1102 7 0.10054388423752414 1102 8 0.15995273345680516 1102 9 0.20229360001371788 1102 10-11 0.22532303963080125 1102 12-13 0.284306891468475 1102 14-15 0.3899105732890362 1102 16-17 0.37649135866738703 1102 18-19 0.33520086323336074 1102 20-21 0.1765807069733185 1102 22-23 0.16977932366187787 1102 24-25 0.1802807272935354 1102 26-27 0.20275388273203987 1102 28-29 0.17397542202343175 1102 30-31 0.334520411998561 1102 32-33 0.338753638080739 1102 34-35 0.2694015763464179 1102 36-37 0.1751115000821315 1102 38-39 0.20856901587002596 1102 40-41 0.32222207741300934 1102 42-43 0.43591221121494783 1102 44-45 0.36221797733400507 1102 46-47 0.41438500487874563 1102 48-49 0.5401247918872869 1102 50-51 0.4457813162891 1102 52-53 0.5575101368514765 1102 54-55 0.46416493992147423 1102 56-57 0.3704097953090155 1102 58-59 0.5601799768961371 1102 60-61 0.6681782616328462 1102 62-63 0.5856660632557933 1102 64-65 0.42280411456299305 1102 66-67 0.29263445119114806 1102 68-69 0.3827855405024003 1102 70-71 0.5198777176172307 1102 72-73 0.45359835181223573 1102 74-75 0.47544548468062686 1102 76-77 0.4699913890350196 1102 78-79 0.48539825724126473 1102 80-81 0.5812265096060649 1102 82-83 0.6022102530455378 1102 84-85 0.5159005475424934 1102 86-87 0.6696335592010989 1102 88-89 0.5290216556885134 1102 90-91 0.30119581784054716 1102 92-93 0.4737690783621922 1102 94-95 0.5084042674510378 1102 96-97 0.8445637089292077 1102 98-99 0.7548130220359219 1102 100-101 1.48436116357448 1103 1 0.05745905300969412 1103 2 0.12463149582372068 1103 3 0.08134219224613304 1103 4 0.14387166060191703 1103 5 0.16666361128253726 1103 6 0.122024213332665 1103 7 0.13789386991945207 1103 8 0.013948776463081458 1103 9 0.09493671562562156 1103 10-11 0.11995848205915394 1103 12-13 0.248406284902984 1103 14-15 0.29127127799836927 1103 16-17 0.1437867729802491 1103 18-19 0.18898327413591431 1103 20-21 0.16819210806148277 1103 22-23 0.1229982859923382 1103 24-25 0.19607778818437538 1103 26-27 0.1327342930098041 1103 28-29 0.1637720078146998 1103 30-31 0.20656405607739003 1103 32-33 0.1375222841120305 1103 34-35 0.22687235446840504 1103 36-37 0.23247830949561177 1103 38-39 0.25721856159672996 1103 40-41 0.43286723660390436 1103 42-43 0.36973409443157124 1103 44-45 0.24186223839145526 1103 46-47 0.2979675485488613 1103 48-49 0.22065351914108078 1103 50-51 0.3758404759485856 1103 52-53 0.2661426310591679 1103 54-55 0.11816537597177046 1103 56-57 0.19589400638339782 1103 58-59 0.2767684365322083 1103 60-61 0.24770570026038996 1103 62-63 0.302726205655496 1103 64-65 0.30574559921662114 1103 66-67 0.4419195239529685 1103 68-69 0.39360479440081875 1103 70-71 0.36351170871399674 1103 72-73 0.24217904064154183 1103 74-75 0.20206798227258815 1103 76-77 0.31934479803717863 1103 78-79 0.2879930819099954 1103 80-81 0.3397465744278705 1103 82-83 0.3880579803774289 1103 84-85 0.43665947030301666 1103 86-87 0.46820403013542133 1103 88-89 0.2657964780142912 1103 90-91 0.1990207729338742 1103 92-93 0.278554567649671 1103 94-95 0.3570640636137945 1103 96-97 0.4688801838205201 1103 98-99 0.4474907938839934 1103 100-101 0.6693912966023632 1104 1 0.17119886451550315 1104 2 0.2360207667615697 1104 3 0.22465188864873653 1104 4 0.2180708139563663 1104 5 0.05799690891621623 1104 6 0.07845607020334455 1104 7 0.05578982608778915 1104 8 0.09174490511713174 1104 9 0.06716989826882269 1104 10-11 0.14274695877357857 1104 12-13 0.11605168584771519 1104 14-15 0.2527533445575685 1104 16-17 0.2723488706545112 1104 18-19 0.20026213449013852 1104 20-21 0.1266584930766541 1104 22-23 0.0781773667449599 1104 24-25 0.13666674797153178 1104 26-27 0.19564577852889897 1104 28-29 0.14977621567689425 1104 30-31 0.18369349363869247 1104 32-33 0.07304588534310597 1104 34-35 0.06100000516793358 1104 36-37 0.04418035170387924 1104 38-39 0.06688255266806209 1104 40-41 0.13834295332149082 1104 42-43 0.0590597091232965 1104 44-45 0.07026573094525901 1104 46-47 0.11206099803759884 1104 48-49 0.19370967282247165 1104 50-51 0.276523795565744 1104 52-53 0.2523522293816569 1104 54-55 0.16564791602115037 1104 56-57 -0.016081631419154974 1104 58-59 0.2015574797438191 1104 60-61 0.2829208282718412 1104 62-63 0.06627598694812775 1104 64-65 0.20502923393021888 1104 66-67 0.18326634987768387 1104 68-69 0.13232840014132208 1104 70-71 0.2929116444546125 1104 72-73 0.35417074328970344 1104 74-75 0.23790386155513943 1104 76-77 0.1545201866937056 1104 78-79 0.31139561620985035 1104 80-81 0.47923347171431985 1104 82-83 0.3426208897491527 1104 84-85 0.5700773084821051 1104 86-87 0.6537297451619679 1104 88-89 0.5053730002288681 1104 90-91 0.46378070213582134 1104 92-93 0.5608322245496637 1104 94-95 0.7222528819175729 1104 96-97 0.8019167637558091 1104 98-99 0.793805532393403 1104 100-101 1.3824907689990091 1105 1 0.014800393223801223 1105 2 0.07497383505064192 1105 3 0.06183484152170138 1105 4 0.09872803034306088 1105 5 0.13958053656938318 1105 6 0.10007474919368065 1105 7 0.03018152058417911 1105 8 0.08072746261345287 1105 9 0.21492761219528234 1105 10-11 0.13170223970691808 1105 12-13 0.15096263454564962 1105 14-15 0.2957747789766998 1105 16-17 0.22333593889322145 1105 18-19 0.14867528705806166 1105 20-21 0.16731926495378957 1105 22-23 0.14511843945015812 1105 24-25 0.18849984745125425 1105 26-27 0.04532769184055496 1105 28-29 -0.0025767407591956726 1105 30-31 0.12848477643064626 1105 32-33 0.11330361262172772 1105 34-35 0.11560730277393105 1105 36-37 0.14232276054102755 1105 38-39 0.06457091164136841 1105 40-41 0.21924733733276724 1105 42-43 0.18459160210150571 1105 44-45 0.15444196729839632 1105 46-47 0.030198310163122244 1105 48-49 0.18172305397943234 1105 50-51 0.06522618651862189 1105 52-53 0.21870725654759582 1105 54-55 0.23304944762876545 1105 56-57 0.1446643043729523 1105 58-59 0.09182138716678878 1105 60-61 0.12649965830185295 1105 62-63 0.1738929004009293 1105 64-65 0.23114568463518026 1105 66-67 -0.05450154221241377 1105 68-69 0.207696786337781 1105 70-71 0.2465300426680166 1105 72-73 0.38291127565191374 1105 74-75 0.41820499481049467 1105 76-77 0.2344527239328933 1105 78-79 0.2026624618718067 1105 80-81 0.17712417319122054 1105 82-83 0.5107435144946031 1105 84-85 0.6033974422478963 1105 86-87 0.7965980874870304 1105 88-89 0.7135679479227228 1105 90-91 0.5670816240454997 1105 92-93 0.50825731700386 1105 94-95 0.3736473437452652 1105 96-97 0.6446641354777576 1105 98-99 0.5300846582310577 1105 100-101 0.9045247002803869 1106 1 0.14511834923833078 1106 2 0.19280739813866177 1106 3 0.11455436484718007 1106 4 0.1739871425743189 1106 5 0.28726153326167747 1106 6 -0.018504830952309703 1106 7 -0.011617798295496584 1106 8 0.02647581941383237 1106 9 0.2761073402788128 1106 10-11 0.15137253223590363 1106 12-13 0.25950558474774255 1106 14-15 0.22460631288144128 1106 16-17 0.08793742949269046 1106 18-19 0.08045628885601985 1106 20-21 0.038485114925876474 1106 22-23 0.10040066639547263 1106 24-25 0.12541034945575547 1106 26-27 0.015371393756097973 1106 28-29 -0.23336521918398034 1106 30-31 0.011034559777677089 1106 32-33 -0.09309563562262468 1106 34-35 -0.09390302226250213 1106 36-37 -0.29350416012588454 1106 38-39 -0.10100078089327269 1106 40-41 0.19751368314458517 1106 42-43 0.30248677067056207 1106 44-45 0.27514377187483774 1106 46-47 0.29012723600404655 1106 48-49 0.1296567175350276 1106 50-51 0.011028342626069332 1106 52-53 -0.3184277678983847 1106 54-55 -0.09021244444433307 1106 56-57 -0.16398843861108503 1106 58-59 -0.056812595682416145 1106 60-61 -0.11175785176494912 1106 62-63 -0.09405486490244641 1106 64-65 -0.153139179109111 1106 66-67 -0.010627890834456366 1106 68-69 -0.2392220746021927 1106 70-71 -0.017627508708212503 1106 72-73 0.1346977291129825 1106 74-75 0.18600275498710772 1106 76-77 0.057917787506791996 1106 78-79 -0.08307283119095032 1106 80-81 0.0849885348002033 1106 82-83 0.04683926291469831 1106 84-85 0.5024693092610732 1106 86-87 0.5514156572014386 1106 88-89 0.31631048891252433 1106 90-91 0.2776105492956873 1106 92-93 0.4443933199642984 1106 94-95 0.33096508329670016 1106 96-97 0.5289165086601351 1106 98-99 0.6824985171013402 1106 100-101 1.2782469667375942 1107 1 0.09607286686195238 1107 2 0.10078851797197075 1107 3 0.08556700340987788 1107 4 0.08115721942920828 1107 5 0.06452400047726314 1107 6 0.023096419196193096 1107 7 0.038396366398778525 1107 8 0.09609574868174775 1107 9 0.0881962214507368 1107 10-11 0.04574983399871968 1107 12-13 0.022215656302293496 1107 14-15 0.11100226634813737 1107 16-17 0.24291540567772785 1107 18-19 0.17501179161127567 1107 20-21 0.20162172654823252 1107 22-23 0.14505692125030123 1107 24-25 0.22414877781438491 1107 26-27 0.21054283224934522 1107 28-29 0.2812673669236503 1107 30-31 0.1557943082858131 1107 32-33 0.07653457447177914 1107 34-35 0.20924016908881526 1107 36-37 0.05444812770070229 1107 38-39 0.07306800468981578 1107 40-41 0.1276166284871465 1107 42-43 0.13825605064716484 1107 44-45 0.1641001080624349 1107 46-47 0.18523630414993164 1107 48-49 -0.034237019842400684 1107 50-51 0.1755348172621254 1107 52-53 0.2505940457307858 1107 54-55 0.2003947640142698 1107 56-57 0.04717745280511565 1107 58-59 0.19667785914134583 1107 60-61 0.38238915948456054 1107 62-63 0.4112918502592606 1107 64-65 0.13797658756639208 1107 66-67 0.23190640143344154 1107 68-69 0.2957171999826933 1107 70-71 0.20123575173025543 1107 72-73 0.4020081278262815 1107 74-75 0.30762104846129645 1107 76-77 0.16936560203154372 1107 78-79 0.2660112614004575 1107 80-81 0.4476848985861075 1107 82-83 0.4431525585364362 1107 84-85 0.6578683424064593 1107 86-87 0.5127069747007731 1107 88-89 0.275511143230009 1107 90-91 0.38489005983696245 1107 92-93 0.6150223694676207 1107 94-95 0.6773295545880629 1107 96-97 1.0182968718246208 1107 98-99 0.8704720062767741 1107 100-101 1.2299997334222326 1108 1 -0.00653367803911209 1108 2 0.09114239925051493 1108 3 0.03537040269957714 1108 4 -0.014718393626765192 1108 5 0.15359680615965488 1108 6 0.014511521513121295 1108 7 0.006785931763225506 1108 8 0.029640480628302157 1108 9 0.03312899669172964 1108 10-11 -0.05234017953078762 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-1.2654420282050012 2316 54-55 -1.1851250477281567 2316 56-57 -1.3557029405375118 2316 58-59 -1.6002103695022 2316 60-61 -1.165174140807295 2316 62-63 -0.9268730328685493 2316 64-65 -1.1286359594322697 2316 66-67 -1.1413865283906546 2316 68-69 -1.6711655071400706 2316 70-71 -1.5651828847170428 2316 72-73 -1.1074317007436463 2316 74-75 -1.1009169317138117 2316 76-77 -1.0503869812513926 2316 78-79 -1.220040982703349 2316 80-81 -1.1163312189959917 2316 82-83 -1.7188810701544384 2316 84-85 -1.1771078624682545 2316 86-87 -1.2862906063472614 2316 88-89 -1.2251935079939251 2316 90-91 -1.1647701037052265 2316 92-93 -1.4967180927625456 2316 94-95 -1.461204744573191 2316 96-97 -1.7349654267056067 2316 98-99 -2.443278552448543 2316 100-101 -3.255527308883135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 10.0 5 52.0 6 92.0 7 234.0 8 553.0 9 1074.0 10 1778.0 11 2841.0 12 4266.0 13 5930.0 14 7643.0 15 10469.0 16 13635.0 17 17529.0 18 15455.0 19 9458.0 20 8296.0 21 8184.0 22 8717.0 23 9955.0 24 11302.0 25 13649.0 26 16647.0 27 20469.0 28 25647.0 29 31658.0 30 38705.0 31 48251.0 32 60156.0 33 76914.0 34 102634.0 35 138662.0 36 197416.0 37 256042.0 38 153735.0 39 8765.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.079618866701345 19.304522188802704 14.939279495239601 21.67657944925635 2 15.876719019951334 20.970686582825028 39.38881133218958 23.763783065034055 3 20.24803417875707 24.87359347299106 30.03251745721625 24.845854891035614 4 13.108140292064105 17.08588577905869 36.90572732644374 32.90024660243346 5 13.420857662046757 37.83061864209413 33.817030991269924 14.931492704589186 6 31.441433429401762 36.5309247892725 16.93304050550484 15.094601275820901 7 28.41218782784859 33.477486222822485 20.25930939259831 17.851016556730617 8 26.926920665163333 34.03693911512535 19.226624018715256 19.809516200996057 9 26.52212186621969 17.017075258962702 20.49671699205325 35.96408588276436 10-11 25.349554427387943 26.793407153279393 26.487528187441967 21.369510231890697 12-13 26.84330520833726 24.22798204723341 27.4793019373462 21.449410807083126 14-15 23.09290681281958 25.53802127552937 26.794362048778687 24.57470986287236 16-17 21.644467155199116 28.90292735095879 27.430094714398447 22.022510779443653 18-19 21.497178569032922 26.918117076384018 30.526435982873195 21.05826837170987 20-21 22.384823398893 25.549240597151712 30.927314083471003 21.138621920484283 22-23 21.97932517404251 25.62526802584504 30.883172009095354 21.512234791017093 24-25 22.15525401859467 25.790680357422797 31.029926923679863 21.024138700302675 26-27 21.765933721807045 25.963123009097338 31.091978431079205 21.17896483801641 28-29 21.321867690379403 26.511102426921223 31.05257782891149 21.11445205378788 30-31 21.559701481288315 25.802747626275796 31.058215972348886 21.579334920087003 32-33 21.37612925911256 26.14356152240956 31.03084006589284 21.449469152585042 34-35 22.234800408799437 25.96652728495069 30.618806582997443 21.179865723252426 36-37 22.101453098385615 25.698738336775147 30.83730272380579 21.36250584103345 38-39 21.669563945962505 26.121940474947795 30.83550550771978 21.37299007136992 40-41 21.867453807124964 26.23825394097803 30.577475000527578 21.316817251369432 42-43 21.706533569298767 26.416056402572302 30.06780488834353 21.8096051397854 44-45 21.96703593623345 26.279451782227802 29.921537282885964 21.831974998652782 46-47 22.51623610312422 26.720552114179164 29.276838653419023 21.48637312927759 48-49 22.652889338586505 26.914100846574605 28.77697950170238 21.656030313136508 50-51 22.16773977656503 27.242508265948363 28.96669427635362 21.623057681132988 52-53 22.63449170918031 27.424483486568352 28.31163623948527 21.62938856476607 54-55 22.10397632804292 27.172177913509326 28.51533235554363 22.208513402904124 56-57 22.367917189309146 27.358373938633733 28.284544155248938 21.989164716808183 58-59 23.043475966388076 26.939222004261048 28.138640897323196 21.878661132027677 60-61 22.373113771868262 27.08777005751125 28.964292364323875 21.57482380629661 62-63 22.276621694760916 27.165488472085908 28.91811886875229 21.639770964400885 64-65 22.159037583238444 27.40224441788403 28.61973916265339 21.818978836224133 66-67 22.115921264014574 27.480642167157765 28.213652741186706 22.189783827640955 68-69 22.13784269708651 27.2537240651352 28.54505488542251 22.063378352355784 70-71 22.373836607676488 27.31922905027091 28.25642451826947 22.050509823783134 72-73 22.474284611766976 27.10143409263569 28.444369746885815 21.979911548711517 74-75 22.071171214966373 28.02987908256957 27.99528494751904 21.90366475494502 76-77 22.313740637806774 27.457534504686986 28.006293278063026 22.222431579443214 78-79 22.408994411281306 27.42429375954678 27.96066603326096 22.206045795910953 80-81 22.516673608114143 27.611947330432372 27.76014880453198 22.111230256921502 82-83 22.390859012364757 27.6487361035466 27.94932729763805 22.011077586450593 84-85 22.20778858499507 27.702907996746024 27.85275227179 22.236551146468912 86-87 22.1975946189294 27.72572731099864 27.65818879953688 22.41848927053508 88-89 22.180193025985254 27.806517068197767 27.341276109952194 22.672013795864785 90-91 22.24057147101529 27.518179895281087 27.58653713102457 22.654711502679053 92-93 22.485264426085397 27.392711903508943 27.72576075368936 22.3962629167163 94-95 22.18477599933351 27.254416549521558 27.78874147894234 22.772065972202586 96-97 22.034833278597226 27.20929943188824 28.107222941353687 22.648644348160847 98-99 21.967146601991537 27.476259641692536 27.42592073073245 23.13067302558348 100-101 21.54319265114034 27.685870942439966 27.46157385060939 23.309362555810306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 394.0 1 299.5 2 284.0 3 376.0 4 331.0 5 289.0 6 494.5 7 1008.5 8 1591.0 9 1915.0 10 1879.5 11 1772.5 12 1821.5 13 1948.0 14 2102.0 15 2312.5 16 2552.0 17 2756.5 18 2901.0 19 3110.5 20 3420.5 21 3888.0 22 4453.0 23 5025.5 24 5798.5 25 6900.0 26 8415.0 27 10271.0 28 12179.0 29 14528.0 30 17306.5 31 20017.5 32 22609.0 33 24937.0 34 27302.5 35 29192.5 36 31495.0 37 34217.0 38 35624.5 39 36521.0 40 37667.5 41 39575.5 42 41309.0 43 42617.0 44 43864.0 45 45586.0 46 46696.0 47 50245.0 48 59244.0 49 58409.0 50 49641.5 51 45210.5 52 42701.0 53 41523.0 54 39974.0 55 37271.0 56 34293.5 57 31231.0 58 28458.0 59 25198.0 60 21493.0 61 18213.5 62 14878.5 63 11595.5 64 8848.5 65 6804.5 66 5131.0 67 3980.5 68 2924.0 69 1900.0 70 1381.0 71 1077.0 72 880.5 73 767.5 74 608.5 75 501.0 76 379.0 77 230.0 78 160.0 79 105.5 80 58.5 81 50.5 82 45.5 83 27.0 84 16.0 85 9.0 86 5.5 87 2.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01974628287529996 2 0.015374968345653404 3 0.01205879870247326 4 0.0 5 0.002939332183727857 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012812473621377839 14-15 1.8841872972614467E-4 16-17 7.536749189045788E-5 18-19 0.0017334523134805312 20-21 0.0 22-23 7.536749189045788E-5 24-25 0.0 26-27 0.001168196124302097 28-29 0.0020726060269875913 30-31 0.0010551448864664103 32-33 0.009986192675485667 34-35 0.0015073498378091574 36-37 9.044099026854945E-4 38-39 9.420936486307234E-4 40-41 3.014699675618315E-4 42-43 0.006330869318798462 44-45 0.0017711360594257599 46-47 3.7683745945228935E-4 48-49 0.0010928286324116392 50-51 2.2610247567137363E-4 52-53 2.2610247567137363E-4 54-55 0.0022610247567137363 56-57 0.01808819805370989 58-59 0.029996261772402236 60-61 0.023288554994151483 62-63 0.01703305316724348 64-65 0.02234646134552076 66-67 0.0032784858972349173 68-69 0.001695768567535302 70-71 7.536749189045788E-5 72-73 0.0 74-75 0.0013189311080830128 76-77 0.0014696660918639285 78-79 0.01066450010249979 80-81 0.019557864145573818 82-83 0.021065213983382975 84-85 0.034292208810158334 86-87 0.013679199778118104 88-89 0.04804677608016689 90-91 0.05354860298817032 92-93 0.03391537135070604 94-95 0.036590917312817293 96-97 0.05822138748537871 98-99 0.06180134335017545 100-101 0.06873515260409757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1326832.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.2512345305589 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21403851528795 48.93246081758458 2 11.284253155289916 13.597803667231437 3 3.2657524924155092 5.902968580178522 4 1.33961968464271 3.2285495920464506 5 0.7207005997629018 2.171155043131453 6 0.4440777519672372 1.6053739670148819 7 0.30549386024390496 1.288446755484091 8 0.22737882457628456 1.0959883909462822 9 0.16399429153146539 0.8892772668661653 >10 0.924853517145914 10.33407441005282 >50 0.06831449118674697 2.8494499116736 >100 0.037645705198629247 4.399016865207617 >500 0.0020010894197849904 0.754078160147807 >1k 0.0015008170648387427 1.475075445677358 >5k 3.7520426620968566E-4 1.476281126756839 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7518 0.5666128040324623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6897 0.5198095915684879 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5176 0.3901021380250099 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4009 0.3021482749888456 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2405 0.1812588179965512 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2030 0.15299600853762946 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1459 0.10996117066817802 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1423 0.10724794096012155 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.5073498378091575E-4 0.0 0.0 3.768374594522894E-5 0.0 12-13 1.5073498378091575E-4 0.0 0.0 7.536749189045788E-5 0.0 14-15 1.5073498378091575E-4 0.0 0.0 7.536749189045788E-5 0.0 16-17 1.5073498378091575E-4 0.0 0.0 7.536749189045788E-5 0.0 18-19 1.5073498378091575E-4 0.0 0.0 1.5073498378091575E-4 0.0 20-21 1.5073498378091575E-4 0.0 0.0 1.8841872972614467E-4 0.0 22-23 1.5073498378091575E-4 0.0 0.0 2.637862216166026E-4 0.0 24-25 1.8841872972614467E-4 0.0 0.0 4.5220495134274725E-4 0.0 26-27 2.2610247567137363E-4 0.0 0.0 4.898886972879762E-4 0.0 28-29 2.637862216166026E-4 0.0 0.0 0.0013566148540282417 0.0 30-31 3.014699675618315E-4 0.0 0.0 0.0033161696431801466 0.0 32-33 3.014699675618315E-4 0.0 0.0 0.006971492999867353 0.0 34-35 3.391537135070604E-4 0.0 0.0 0.00987314143764998 0.0 36-37 3.7683745945228935E-4 0.0 0.0 0.013264678572720585 0.0 38-39 4.5220495134274725E-4 0.0 0.0 0.01699536942129825 0.0 40-41 4.5220495134274725E-4 0.0 0.0 0.023514657469822856 0.0 42-43 4.5220495134274725E-4 0.0 0.0 0.030222364248073605 0.0 44-45 4.5220495134274725E-4 0.0 0.0 0.03790984842090031 0.0 46-47 4.898886972879762E-4 0.0 0.0 0.048800450999071474 0.0 48-49 5.275724432332052E-4 0.0 0.0 0.057919917517816874 0.0 50-51 5.275724432332052E-4 0.0 0.0 0.07035555367974242 0.0 52-53 5.275724432332052E-4 0.0 0.0 0.09628197089005994 0.0 54-55 5.275724432332052E-4 0.0 0.0 0.13147858960290376 0.0 56-57 5.275724432332052E-4 0.0 0.0 0.16946380551569454 0.0 58-59 5.275724432332052E-4 0.0 0.0 0.22448207459572878 0.0 60-61 5.275724432332052E-4 0.0 0.0 0.2939709021187309 0.0 62-63 5.275724432332052E-4 0.0 0.0 0.3387015085557177 0.0 64-65 5.275724432332052E-4 0.0 0.0 0.37804333932253664 0.0 66-67 5.275724432332052E-4 0.0 0.0 0.48039239330977845 0.0 68-69 5.275724432332052E-4 0.0 0.0 0.589788307788778 0.0 70-71 5.652561891784341E-4 0.0 0.0 0.638324972566233 0.0 72-73 6.02939935123663E-4 0.0 0.0 0.7310646713374414 0.0 74-75 6.02939935123663E-4 0.0 0.0 0.8643520807457161 0.0 76-77 6.02939935123663E-4 0.0 0.0 1.0046109831538583 0.0 78-79 6.783074270141208E-4 0.0 0.0 1.1443799968647124 0.0 80-81 6.783074270141208E-4 0.0 0.0 1.300353021332015 3.768374594522894E-5 82-83 6.783074270141208E-4 0.0 0.0 1.4459253319184344 7.536749189045788E-5 84-85 6.783074270141208E-4 0.0 0.0 1.6079654394829186 7.536749189045788E-5 86-87 6.783074270141208E-4 0.0 0.0 1.866588987905025 1.5073498378091575E-4 88-89 6.783074270141208E-4 0.0 0.0 2.2508501453085246 1.5073498378091575E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1145 0.0 26.963556 2 GTACCTG 1290 0.0 24.676603 1 GTACATG 6675 0.0 23.631273 1 GAGTACT 7710 0.0 22.943485 12-13 TACATGG 6935 0.0 22.464497 2 ACATGGG 6725 0.0 21.821592 3 TAAGGTG 505 0.0 21.628376 5 GTACTTT 8290 0.0 21.395554 14-15 GTATCAA 14325 0.0 21.26002 1 GGTATCA 11135 0.0 21.0357 1 AGTACTT 7990 0.0 20.594152 12-13 CATGGGG 2875 0.0 20.316772 4 ACTTTTT 9150 0.0 19.592205 16-17 AGAGTAC 12225 0.0 18.975933 10-11 GTAAGGT 505 0.0 18.807283 4 TACTTTT 8855 0.0 18.5556 14-15 CAACGCA 16535 0.0 18.352032 5 TATCAAC 16635 0.0 18.330807 2 TCAACGC 16565 0.0 18.318794 4 ATCAACG 16650 0.0 18.255173 3 >>END_MODULE