FastQCFastQC Report
Thu 26 May 2016
SRR936663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108003
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121160.574761990376674No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT87580.4154643043676883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72810.3453979904203172No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT60500.28700148908706485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.272295627662769No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55360.2626182220803291No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37450.1776562936580261No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37290.1768972814554818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.16110033999002848No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31230.14814969428411628No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29480.13984799831878797No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28450.13496185726490903No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25910.1229125385495182No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23800.1129030651284652No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21490.10194482645423181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG102800.023.7990321
TACATGG100550.023.6223322
GAGTACT76100.022.9410212-13
ACATGGG100150.022.7696343
GTACTTT80550.021.38002614-15
GTGTAAG10000.019.9523681
AGTACTT79550.019.91570512-13
GTGTAGC14100.019.5413251
GTATAGG4900.019.3900571
TACCTGG15450.019.370772
CATGGGA54200.019.1087954
TAGCCCT15500.019.0036074
GTATAGC8900.018.6819921
ACTTTTT96250.018.53424616-17
CATGGGG43600.018.524164
TATACGT1559.615878E-418.3905894
TATACCG1300.00755481618.2735675
GTACCTG18950.018.0496351
TAAGGTG8050.017.7060665
ATGGGTA7550.017.6200755
Produced by FastQC (version 0.11.5)