FastQCFastQC Report
Thu 26 May 2016
SRR936663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108003
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT156450.742171619300352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.39544535752558224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73120.34686857656274683No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65450.3104834291032793No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53680.25464859395361394No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45080.2138516880668576No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43970.20858604091170652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.20369989985782752No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC41040.1946866299526139No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39810.18885172364555458No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG32550.1544115449551068No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31980.1517075639835427No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28180.1336810241731155No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24510.11627118177725553No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24090.11427877474557674No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23800.1129030651284652No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.10550269615365823No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21430.10166019687827768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT92700.023.18605212-13
TACATGG104400.023.0716082
GTACATG108300.022.6832161
GTACTTT95600.022.18512214-15
ACATGGG105850.021.9012283
AGTACTT96050.021.21523712-13
TACCTGG16400.020.5676612
TAAGGTG8250.020.1507765
CATGGGA56900.020.034894
ACTTTTT112250.019.14785816-17
GTAAGGT7050.018.8650044
TACTTTT107150.018.81844714-15
GTGTAGC13400.018.7937581
GTACCTG19800.017.5186331
AGAGTAC180600.017.31871810-11
GTAAGCG2350.017.1796194-95
ATGGGAT22700.016.9487175
CATGGGG45100.016.8512234
ATGGGTA10200.016.298425
AGTACAT82750.016.0179392
Produced by FastQC (version 0.11.5)