##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936663_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2108003 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98076757955278 31.0 31.0 34.0 30.0 34.0 2 32.084630809348944 33.0 31.0 34.0 30.0 34.0 3 32.212351215818956 34.0 31.0 34.0 30.0 34.0 4 35.91810542964123 37.0 35.0 37.0 35.0 37.0 5 35.79637173191879 37.0 35.0 37.0 35.0 37.0 6 35.85967050331522 37.0 35.0 37.0 35.0 37.0 7 35.79435608013841 37.0 35.0 37.0 33.0 37.0 8 35.769981826401576 37.0 35.0 37.0 33.0 37.0 9 37.38877648656098 39.0 37.0 39.0 34.0 39.0 10-11 37.28701714371374 39.0 37.0 39.0 34.0 39.0 12-13 37.16831119310551 39.0 37.0 39.0 33.0 39.0 14-15 38.2518706092923 40.0 38.0 41.0 33.0 41.0 16-17 38.24502550518192 40.0 37.5 41.0 33.0 41.0 18-19 38.40935022388488 40.0 38.0 41.0 33.5 41.0 20-21 38.42248232094546 40.0 38.0 41.0 34.0 41.0 22-23 38.32186434269781 40.0 38.0 41.0 34.0 41.0 24-25 38.20209743534521 40.0 38.0 41.0 33.0 41.0 26-27 37.9902770536854 40.0 37.0 41.0 33.0 41.0 28-29 38.02146273036614 40.0 37.0 41.0 33.0 41.0 30-31 37.9113023083933 40.0 37.0 41.0 33.0 41.0 32-33 37.718841956107276 40.0 37.0 41.0 33.0 41.0 34-35 37.52976774700984 40.0 36.0 41.0 33.0 41.0 36-37 37.34690130896398 39.5 36.0 41.0 32.0 41.0 38-39 37.13653538443731 39.0 36.0 41.0 31.0 41.0 40-41 36.93516778676312 39.0 36.0 41.0 31.0 41.0 42-43 36.66227965519973 39.0 35.0 40.5 30.5 41.0 44-45 36.36837186664346 39.0 35.0 40.0 30.0 41.0 46-47 35.98196468411098 39.0 35.0 40.0 29.5 41.0 48-49 35.563271731586724 38.0 35.0 40.0 28.0 41.0 50-51 35.25806035380405 38.0 34.5 40.0 27.0 41.0 52-53 34.997747631288945 38.0 34.0 40.0 26.5 41.0 54-55 34.673605066026944 38.0 34.0 40.0 26.0 41.0 56-57 34.30722133697154 38.0 33.5 40.0 25.5 41.0 58-59 33.939705730969074 37.0 33.0 40.0 24.0 41.0 60-61 33.53107846620712 36.5 32.5 39.0 23.0 40.5 62-63 33.20712636556969 36.0 32.0 39.0 23.0 40.0 64-65 32.88401036431162 36.0 32.0 39.0 22.0 40.0 66-67 32.420013159374065 35.0 31.0 38.5 21.0 40.0 68-69 31.94104918256758 35.0 31.0 38.0 19.5 40.0 70-71 31.422893610682717 35.0 30.5 37.0 18.0 39.5 72-73 30.8449783989871 34.0 30.0 36.5 15.5 39.0 74-75 30.31721112351358 34.0 29.0 36.0 12.0 39.0 76-77 28.952081424931556 32.5 28.0 34.5 11.0 36.5 78-79 29.733656925535684 34.0 29.0 35.0 8.0 37.0 80-81 29.566640085426826 34.0 29.0 35.0 7.0 37.0 82-83 29.157257366332022 34.0 29.0 35.0 4.5 36.0 84-85 28.708581534276753 33.0 29.0 35.0 2.0 36.0 86-87 28.30624932696965 33.0 27.5 35.0 2.0 35.5 88-89 27.806642115784463 33.0 27.0 35.0 2.0 35.0 90-91 27.207385852866434 32.5 26.0 34.0 2.0 35.0 92-93 26.621338299803178 32.0 25.0 34.0 2.0 35.0 94-95 25.86455213773415 32.0 24.0 34.0 2.0 35.0 96-97 25.019646082097605 31.0 19.5 34.0 2.0 35.0 98-99 23.86952200732162 31.0 8.5 34.0 2.0 35.0 100-101 20.051080572465978 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18061650238315252 1101 2 0.2921276008547551 1101 3 0.19652822303645934 1101 4 0.11580361517318494 1101 5 0.1047729355642204 1101 6 0.0867554356272251 1101 7 0.07805943095748802 1101 8 0.09622690543199042 1101 9 0.19121160319660646 1101 10-11 0.1185655272103503 1101 12-13 0.21744178286500215 1101 14-15 0.23040170748976863 1101 16-17 0.2475854784581415 1101 18-19 0.23443377789077147 1101 20-21 0.20811872580792823 1101 22-23 0.21368613790360058 1101 24-25 0.21882399471638792 1101 26-27 0.011636163838950608 1101 28-29 0.1137046981001717 1101 30-31 0.20231786114452177 1101 32-33 0.006301757021525134 1101 34-35 0.13426595129956098 1101 36-37 0.22467675571934365 1101 38-39 0.21957276622111976 1101 40-41 0.1399638346051475 1101 42-43 0.17368768446572602 1101 44-45 0.13074064673691055 1101 46-47 0.08978675615712461 1101 48-49 0.0923146949155651 1101 50-51 0.20093230027899978 1101 52-53 0.05586982592045331 1101 54-55 0.20119471392925448 1101 56-57 0.17274050070378877 1101 58-59 0.16063754122951224 1101 60-61 0.08273818641825414 1101 62-63 0.14389968328131175 1101 64-65 0.25718894222816147 1101 66-67 0.23011888764333577 1101 68-69 0.19094839185877888 1101 70-71 0.18668804438705422 1101 72-73 0.08082867228158008 1101 74-75 0.1997528496489167 1101 76-77 0.14953961646117975 1101 78-79 0.1479933905196944 1101 80-81 0.07298566376914906 1101 82-83 0.12865422836500073 1101 84-85 0.18451601351436508 1101 86-87 0.08541292864454064 1101 88-89 -0.20911220213178083 1101 90-91 0.07718770009025988 1101 92-93 0.032998168783102244 1101 94-95 -0.1943520576204989 1101 96-97 0.07341198178756514 1101 98-99 -0.4711181116915668 1101 100-101 -0.06745236224210416 1102 1 0.23951346715524835 1102 2 0.3323704792801081 1102 3 0.20629181534982166 1102 4 0.16683005491255187 1102 5 0.01130657588144146 1102 6 0.04528070912879656 1102 7 0.15960518509951527 1102 8 0.03022898208767799 1102 9 0.15486230369169363 1102 10-11 0.20645260529560971 1102 12-13 0.2450403863892987 1102 14-15 0.24833197399392049 1102 16-17 0.2548092305837031 1102 18-19 0.2927978848535844 1102 20-21 0.25672947325348616 1102 22-23 0.2706011003582631 1102 24-25 0.15349767385804114 1102 26-27 0.3800299158140277 1102 28-29 0.31181569384152397 1102 30-31 0.4069736328929707 1102 32-33 0.22357215865877578 1102 34-35 0.3570614609078575 1102 36-37 0.4343384715184797 1102 38-39 0.2995249128510764 1102 40-41 0.4000219207713016 1102 42-43 0.3197166071629667 1102 44-45 0.4385464643824619 1102 46-47 0.41008561399650745 1102 48-49 0.4141680348701158 1102 50-51 0.3996539531765322 1102 52-53 0.5316190020298848 1102 54-55 0.5445647649427485 1102 56-57 0.6007342707367442 1102 58-59 0.34032649432418083 1102 60-61 0.4584083593224051 1102 62-63 0.36623035307801644 1102 64-65 0.3648406660028556 1102 66-67 0.36172137361086243 1102 68-69 0.23786832022920734 1102 70-71 -0.1473760309744776 1102 72-73 -0.07906192708940551 1102 74-75 0.29401240151263863 1102 76-77 0.11136857828382318 1102 78-79 0.21729605556115317 1102 80-81 0.3650036313552647 1102 82-83 0.37079619336281766 1102 84-85 0.2755302641877755 1102 86-87 0.3086410748531385 1102 88-89 0.15680069871543267 1102 90-91 0.1755325453192178 1102 92-93 0.37487031902462675 1102 94-95 0.49590090311867385 1102 96-97 0.6543512922776848 1102 98-99 0.337047057676255 1102 100-101 -0.17894650095668396 1103 1 0.27997994406859306 1103 2 0.27807256656663526 1103 3 0.2301042758178795 1103 4 0.1450432111427844 1103 5 0.14136033932229708 1103 6 0.175682353657578 1103 7 0.13244199724373118 1103 8 0.2612821130174652 1103 9 0.17082688307183247 1103 10-11 0.18479621693381176 1103 12-13 0.16359984242977532 1103 14-15 0.31845610429119375 1103 16-17 0.2247815582244428 1103 18-19 0.22465114228558747 1103 20-21 0.18413854978732758 1103 22-23 0.25569202382442313 1103 24-25 0.24324546006930348 1103 26-27 0.18221445091840138 1103 28-29 0.23972761667517517 1103 30-31 0.38924830082466144 1103 32-33 0.28431140597060534 1103 34-35 0.2913886272582715 1103 36-37 0.3029263905570687 1103 38-39 0.2413370570635962 1103 40-41 0.29624741096738916 1103 42-43 0.40840177477840456 1103 44-45 0.3193639849390806 1103 46-47 0.31134510166253193 1103 48-49 0.45567499249187904 1103 50-51 0.4091139468514271 1103 52-53 0.3325274460526515 1103 54-55 0.3478522412235847 1103 56-57 0.4156797166570456 1103 58-59 0.23133930267332659 1103 60-61 0.157348112643092 1103 62-63 0.3877997711678276 1103 64-65 0.38436707333998754 1103 66-67 0.2409331066911875 1103 68-69 0.3098306858206108 1103 70-71 0.13318295068270203 1103 72-73 0.4082491102287449 1103 74-75 0.27603880884976917 1103 76-77 0.20286367632303737 1103 78-79 0.17390026175975848 1103 80-81 0.4575937641825867 1103 82-83 0.26375081069361883 1103 84-85 0.38202378094931433 1103 86-87 0.3959054644799558 1103 88-89 0.2110693577919669 1103 90-91 0.3086911474697551 1103 92-93 0.4325410970132424 1103 94-95 0.6322916368315106 1103 96-97 0.5087504067621893 1103 98-99 0.3969656218761308 1103 100-101 0.34355666159865805 1104 1 0.2173728214232753 1104 2 0.24253412368523897 1104 3 0.16557433537537491 1104 4 0.1635188356880235 1104 5 0.01371259091903454 1104 6 0.1392882770682533 1104 7 0.14509931257506992 1104 8 0.16414612789336758 1104 9 0.11540876789140952 1104 10-11 0.1911260536223054 1104 12-13 0.19093060212470903 1104 14-15 0.17082644546671588 1104 16-17 0.20582303961609227 1104 18-19 0.017833021715347286 1104 20-21 0.05082795475134816 1104 22-23 0.14400470741512095 1104 24-25 0.050595319934018335 1104 26-27 0.03747205056266978 1104 28-29 0.17377156390885062 1104 30-31 0.1759342697793045 1104 32-33 0.2388353165535122 1104 34-35 0.24344703268042167 1104 36-37 0.08886940669956545 1104 38-39 0.2644627476341057 1104 40-41 0.35170898156053454 1104 42-43 0.2767670764734973 1104 44-45 0.2834425280710988 1104 46-47 0.1850961796262638 1104 48-49 0.2554152706065196 1104 50-51 0.3174656392544719 1104 52-53 0.18844833025239893 1104 54-55 0.1385369503081293 1104 56-57 0.30753755345333644 1104 58-59 0.38955692333788505 1104 60-61 0.172287174006172 1104 62-63 0.12068659171382023 1104 64-65 0.22747568492270176 1104 66-67 0.42077636402808594 1104 68-69 0.30593014144892194 1104 70-71 0.04535972999657645 1104 72-73 0.2984347557942719 1104 74-75 0.3179256159212116 1104 76-77 0.10980289938068566 1104 78-79 0.4277629089691821 1104 80-81 0.20927067150465817 1104 82-83 0.11267638403557712 1104 84-85 0.28557449240537736 1104 86-87 0.24710537285303857 1104 88-89 0.12653729510837408 1104 90-91 0.2541123281095281 1104 92-93 0.21764195399440212 1104 94-95 0.13816637070921445 1104 96-97 0.23354289874483314 1104 98-99 0.08146938801190018 1104 100-101 0.23862733559547777 1105 1 0.09532255536937129 1105 2 0.2562530863186012 1105 3 0.12948158369776763 1105 4 0.06647601264769776 1105 5 0.13287605032348182 1105 6 0.12367057911566803 1105 7 0.09409635521767257 1105 8 0.10222999847695036 1105 9 0.1712100782226713 1105 10-11 0.11269238846589502 1105 12-13 0.10814714961293959 1105 14-15 0.12226497700073224 1105 16-17 0.17406176096950787 1105 18-19 0.270885379030517 1105 20-21 0.13622043162387598 1105 22-23 0.07069977481724976 1105 24-25 0.20741627801676543 1105 26-27 0.09081761750378092 1105 28-29 0.03216168969126443 1105 30-31 0.03359922049302355 1105 32-33 0.12183709746367555 1105 34-35 -0.05185015557458428 1105 36-37 0.19154636547519743 1105 38-39 0.09715291437565554 1105 40-41 0.1889309962805541 1105 42-43 0.13622827446487662 1105 44-45 0.15692320600783916 1105 46-47 0.20670103770378034 1105 48-49 0.11130012312658977 1105 50-51 0.2759932577968698 1105 52-53 0.27186547642958203 1105 54-55 0.23273780521717669 1105 56-57 0.2800011999706413 1105 58-59 0.4382886338236034 1105 60-61 0.43378378811164 1105 62-63 0.32586106497399925 1105 64-65 0.24839506400395805 1105 66-67 0.1747087110944534 1105 68-69 0.2585234678174011 1105 70-71 0.09640536063184157 1105 72-73 0.16816036571540494 1105 74-75 0.269899412904671 1105 76-77 0.13918927027552286 1105 78-79 0.15247921737879366 1105 80-81 0.3040596598141647 1105 82-83 0.29653794578733184 1105 84-85 0.28666523221385987 1105 86-87 0.3928137763311348 1105 88-89 0.27127323503017564 1105 90-91 0.4299745899556484 1105 92-93 0.41520064553968083 1105 94-95 0.6033277442654992 1105 96-97 1.0110548030556465 1105 98-99 0.7878834613521946 1105 100-101 0.4519843422229215 1106 1 0.0505597774172486 1106 2 0.2200348555719387 1106 3 0.16970503248852964 1106 4 0.13668537025806415 1106 5 0.08786877911030899 1106 6 0.12446846400282396 1106 7 0.19394293159412257 1106 8 0.112342830476031 1106 9 0.11644768998296229 1106 10-11 0.11307122995344798 1106 12-13 0.19768716589882018 1106 14-15 0.24828504924110462 1106 16-17 0.20242779937874644 1106 18-19 0.11596474704005288 1106 20-21 0.06241854090334442 1106 22-23 0.12778002890973994 1106 24-25 0.16593887826002884 1106 26-27 0.15062708915630196 1106 28-29 0.2429402120248625 1106 30-31 0.24290011744690787 1106 32-33 0.16372410547739236 1106 34-35 0.16613302115588624 1106 36-37 0.17197603366584957 1106 38-39 0.3131403533292527 1106 40-41 0.340157667377639 1106 42-43 0.5068419074255246 1106 44-45 0.3436053996374753 1106 46-47 0.4804481635747351 1106 48-49 0.33450544618791866 1106 50-51 0.19547262252461906 1106 52-53 0.18068497599782063 1106 54-55 0.2710191529657706 1106 56-57 0.24310117761344685 1106 58-59 0.30242134936020904 1106 60-61 0.229135134362906 1106 62-63 0.28811013633034577 1106 64-65 0.2518296610310742 1106 66-67 0.21554462370862382 1106 68-69 0.4503849394124977 1106 70-71 0.3020413200114973 1106 72-73 0.16200393515292788 1106 74-75 0.05583520470799286 1106 76-77 -0.05026429138826316 1106 78-79 -0.0029452717389979455 1106 80-81 0.1439131894805108 1106 82-83 0.16836225112495384 1106 84-85 0.017757514062083146 1106 86-87 0.10376090056119835 1106 88-89 0.17808889490558855 1106 90-91 0.20539009517677087 1106 92-93 0.23382736053068953 1106 94-95 0.45607296054563307 1106 96-97 0.5524312878086626 1106 98-99 0.5149980101370133 1106 100-101 0.19958207286600782 1107 1 0.05977944860654105 1107 2 0.14281559297612745 1107 3 0.03472309580000399 1107 4 0.03196990700181601 1107 5 -0.047879270368760274 1107 6 0.01581515870007877 1107 7 0.021394481372276175 1107 8 -0.01633913881968141 1107 9 -0.09169679152930144 1107 10-11 0.056962556575321344 1107 12-13 0.10251577297417924 1107 14-15 0.13603714718578175 1107 16-17 0.24995589798716367 1107 18-19 0.15266065758127922 1107 20-21 0.06979240439118684 1107 22-23 0.13802196442959058 1107 24-25 0.1418917463766718 1107 26-27 0.14205655079869928 1107 28-29 0.1465488374512418 1107 30-31 0.03358126420289409 1107 32-33 0.08347038944005192 1107 34-35 0.1342560381281288 1107 36-37 0.1414093599959969 1107 38-39 0.21877421520235174 1107 40-41 0.173226039431448 1107 42-43 0.05014947171320472 1107 44-45 -0.03381844964326319 1107 46-47 -0.04806706746123979 1107 48-49 0.1629071096451682 1107 50-51 0.13116096650323783 1107 52-53 0.1302634912957643 1107 54-55 0.07349107666048837 1107 56-57 0.14125122152832859 1107 58-59 0.14470247863053487 1107 60-61 0.15976055449826987 1107 62-63 -0.15678717333133108 1107 64-65 -0.14889394197842876 1107 66-67 -0.12727824878389526 1107 68-69 -0.04280022525380289 1107 70-71 -0.13330489383009692 1107 72-73 0.1322660460914129 1107 74-75 0.21679728625094086 1107 76-77 -0.05656269756141441 1107 78-79 0.07623599790727908 1107 80-81 0.31246391373030846 1107 82-83 0.3779193018828799 1107 84-85 0.3091526957813606 1107 86-87 0.42328580357600387 1107 88-89 0.37518841641406553 1107 90-91 0.35789672255422644 1107 92-93 0.6403061767871776 1107 94-95 0.7630742055067934 1107 96-97 0.9033416126895517 1107 98-99 1.074625226567111 1107 100-101 0.969101556218277 1108 1 0.04035602199896715 1108 2 0.14623768953054395 1108 3 0.006992650139181933 1108 4 0.08347342513192757 1108 5 0.07490393561741371 1108 6 0.03212598189136884 1108 7 0.13581951241459933 1108 8 0.15523771825540678 1108 9 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2316 54-55 -1.3328926973810198 2316 56-57 -1.2738063223600022 2316 58-59 -1.4923327181211263 2316 60-61 -1.1439445818540648 2316 62-63 -1.321142844134755 2316 64-65 -1.360683560216554 2316 66-67 -1.242866213490096 2316 68-69 -1.3240000965589935 2316 70-71 -1.1531011970739797 2316 72-73 -1.0086162942908352 2316 74-75 -1.2042147901904876 2316 76-77 -1.4316860319900364 2316 78-79 -1.4115874317180577 2316 80-81 -1.4130372048139144 2316 82-83 -1.2963436543117872 2316 84-85 -1.1952508085719096 2316 86-87 -1.3487904597441727 2316 88-89 -1.6818510595085172 2316 90-91 -1.9561187267598577 2316 92-93 -2.0247018734887448 2316 94-95 -2.2152334349140617 2316 96-97 -1.8005377046433964 2316 98-99 -1.3166557457591672 2316 100-101 -1.027902909966567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 25.0 5 47.0 6 128.0 7 336.0 8 754.0 9 1429.0 10 2405.0 11 3921.0 12 5683.0 13 7694.0 14 10182.0 15 13918.0 16 17859.0 17 22636.0 18 20464.0 19 14487.0 20 13515.0 21 14161.0 22 15051.0 23 16717.0 24 19694.0 25 23364.0 26 28603.0 27 34718.0 28 43273.0 29 53433.0 30 66445.0 31 81781.0 32 102827.0 33 132400.0 34 174002.0 35 237709.0 36 331183.0 37 396199.0 38 193817.0 39 7135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03511244017891 19.11357314435394 15.00600405267625 21.845310362790908 2 15.820051750493166 21.079833722837872 38.96070131691437 24.139413209754597 3 20.962873106093852 24.31278076208359 29.356571226336996 25.36777490548556 4 13.215654648956358 16.74876660341556 36.97941176470588 33.0561669829222 5 13.679866483736483 38.05635729832611 33.52888266470833 14.734893553229078 6 31.870732631784684 36.374900794733215 16.735981874788603 15.018384698693502 7 28.801823533737036 33.0652586936876 19.819733917774947 18.313183854800414 8 26.746946913801047 33.748065062295154 18.95112263152477 20.553865392379038 9 26.13308716025886 17.483996694500288 20.24789350294734 36.13502264229351 10-11 25.52159555750158 26.425104708105252 26.36483439539697 21.688465338996195 12-13 27.315852966053654 23.74351459651623 26.762201002560243 22.178431434869875 14-15 23.400632830326522 25.367838557108907 26.345594618570296 24.88593399399428 16-17 22.14153677704211 28.468628782565116 26.80356836612922 22.586266074263552 18-19 21.865319459611776 27.458009284779735 29.150778522993253 21.525892732615233 20-21 23.29870718230734 25.73080786577751 28.939524215574767 22.03096073634038 22-23 22.496353169718144 25.89289822793697 29.107018754788317 22.503729847556563 24-25 22.9887440301614 25.944708057547643 29.25897506751091 21.807572844780047 26-27 22.631351368275293 25.629026179073488 29.884622892641627 21.854999560009592 28-29 21.86673833006879 26.994719646983423 29.364213428538765 21.77432859440902 30-31 22.40116831646063 26.003983414536265 29.031226658753916 22.563621610249193 32-33 22.36788891484903 26.0694543112742 29.203710270608763 22.35894650326801 34-35 23.167257119110733 25.901380842775197 29.048955686654764 21.88240635145931 36-37 22.9724742266085 25.626829944003475 29.53745467241749 21.863241156970535 38-39 22.49896282563065 26.146139944176415 29.509099262983536 21.845797967209396 40-41 22.625600598481547 26.31746229782095 29.083328451141995 21.973608652555512 42-43 22.336041222470936 26.523421869266567 28.325766439995892 22.814770468266605 44-45 22.789701311661208 25.957001270696257 28.423034399884816 22.830263017757726 46-47 23.498092744649792 26.72666974382864 27.860847446611793 21.914390064909774 48-49 23.362784377228902 26.614931060173536 27.784639330462156 22.237645232135407 50-51 22.612317508509353 27.008337385405422 28.20095114980016 22.17839395628506 52-53 23.20239261153815 27.439178420955134 27.198688608268682 22.159740359238032 54-55 22.654704916562974 26.851793432343595 27.5103095497764 22.983192101317034 56-57 23.147055628065154 26.930680668214276 27.180945521967086 22.741318181753485 58-59 23.87928570496726 26.497893097686028 27.086165366629466 22.536655830717248 60-61 23.039219290777986 26.837733425145387 28.11597826208135 22.007069021995278 62-63 22.728107210626185 26.83697817836812 28.029198292220116 22.40571631878558 64-65 22.641274025010752 27.544251277376635 27.453664928234218 22.3608097693784 66-67 22.87180888447076 26.876374389433682 27.154246847043012 23.097569879052543 68-69 22.77217060715652 26.94425027354575 27.36982649858359 22.913752620714142 70-71 23.339424706781493 26.874674735364458 27.03779924939478 22.748101308459276 72-73 23.146909228397618 26.523302677086612 27.69982796036613 22.629960134149645 74-75 22.63699801552191 27.85746105408379 26.907658434458853 22.597882495935448 76-77 23.034485277793063 26.893573538927463 26.927468676054524 23.144472507224954 78-79 23.033629107035733 27.1183377086208 27.074074838408503 22.773958345934968 80-81 23.400720560793552 27.02570256337759 26.887510872574026 22.686066003254833 82-83 23.240473277452526 27.01664991254491 27.177612824659537 22.56526398534303 84-85 22.862278499988378 27.42095782788738 26.979274245157754 22.73748942696649 86-87 22.95578128428181 27.45713624962197 26.691216148103347 22.895866317992873 88-89 22.84480591132444 27.43117259674547 26.268718387592898 23.45530310433719 90-91 22.86504265763494 26.844516324616464 26.74313238882281 23.54730862892578 92-93 23.27672313454732 27.299582272569108 26.796487683433245 22.627206909450326 94-95 22.966783523641006 27.0816512412298 26.924463588536945 23.02710164659225 96-97 22.302126875327474 27.08545570596943 27.417239623272977 23.19517779543012 98-99 22.58611590438748 27.52200855080972 26.35022740884374 23.54164813595906 100-101 22.374518253701687 27.28541297825951 26.51238925290661 23.827679515132193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 298.5 2 251.0 3 317.0 4 290.0 5 259.0 6 426.5 7 905.5 8 1428.5 9 1819.0 10 1887.0 11 1703.0 12 1727.0 13 1908.0 14 2136.0 15 2357.0 16 2547.0 17 2714.0 18 2988.5 19 3389.5 20 3806.5 21 4262.0 22 4887.5 23 5842.0 24 6949.5 25 8385.5 26 10471.0 27 13183.5 28 16191.0 29 19496.5 30 23220.5 31 27206.0 32 31654.0 33 35191.5 34 38102.5 35 41274.0 36 46167.0 37 50569.0 38 52284.0 39 53953.0 40 55980.0 41 58183.0 42 59885.0 43 61947.5 44 65358.5 45 69855.5 46 73939.0 47 86423.5 48 112411.0 49 110982.0 50 86257.5 51 75208.0 52 71832.5 53 70946.0 54 70308.5 55 66663.0 56 59936.5 57 53650.5 58 49884.0 59 45495.0 60 38147.0 61 31473.0 62 26048.5 63 20328.0 64 14549.5 65 10764.5 66 8565.5 67 6829.0 68 5397.0 69 4332.5 70 3418.5 71 2445.5 72 1954.0 73 1688.0 74 1298.5 75 950.5 76 684.5 77 445.5 78 291.5 79 204.5 80 149.5 81 93.0 82 62.0 83 40.0 84 25.5 85 16.5 86 5.0 87 1.0 88 1.0 89 0.5 90 0.0 91 1.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012333948291344937 2 0.00934533774382674 3 0.005882344569718354 4 1.4231478797705694E-4 5 0.004506634952606804 6 0.0 7 3.795061012721519E-4 8 0.0025616661835870256 9 4.743826265901899E-5 10-11 0.0 12-13 0.0 14-15 6.166974145672468E-4 16-17 0.004340601033300238 18-19 0.0017552157183837026 20-21 4.981017579196994E-4 22-23 3.795061012721519E-4 24-25 4.981017579196994E-4 26-27 1.1859565664754747E-4 28-29 0.0 30-31 5.929782832377374E-4 32-33 0.0033443975174608388 34-35 7.115739398852848E-4 36-37 7.115739398852848E-4 38-39 0.005336804549139635 40-41 7.115739398852847E-5 42-43 0.001257113960464003 44-45 0.004909860185208465 46-47 0.0 48-49 0.001968687900349288 50-51 0.0013282713544525316 52-53 6.404165458967564E-4 54-55 2.846295759541139E-4 56-57 0.01119542998752848 58-59 0.002395632264280459 60-61 0.0034867123054378953 62-63 1.4231478797705694E-4 64-65 0.0032969592548018197 66-67 0.0010199226471689082 68-69 0.0017789348497132121 70-71 0.004933579316537974 72-73 0.0027751383655526107 74-75 0.006831109822898734 76-77 0.0014705861424295886 78-79 0.006712514166251186 80-81 0.0029174531535296677 82-83 0.003036048810177215 84-85 0.0021821600823148735 86-87 7.352930712147943E-4 88-89 0.00232447487029193 90-91 0.008254257702669304 92-93 0.00234819400162144 94-95 6.166974145672468E-4 96-97 2.1347218196558543E-4 98-99 7.352930712147943E-4 100-101 0.0022295983449738925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2108003.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.23753435268053 #Duplication Level Percentage of deduplicated Percentage of total 1 77.9336162216298 43.827944194925266 2 13.035266354220893 14.661424787836763 3 3.978110462070103 6.711573713082758 4 1.7611317666831396 3.9616683291376007 5 0.881773352188737 2.479437959249618 6 0.5432251589017916 1.8329786120987905 7 0.3389994609196346 1.3345145680305692 8 0.24926172394126228 1.1214291810364085 9 0.1717870186727259 0.8694790527556793 >10 0.9826553003927174 10.264961748053256 >50 0.07838946574010781 3.014083352502493 >100 0.040652165442186354 4.2098670992533425 >500 0.0026078364771292112 0.9777377261671547 >1k 0.0021030939331686932 2.6888615379317704 >5k 3.3649502930699094E-4 1.3038868471908214 >10k+ 8.412375732674773E-5 0.7401512907478104 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15645 0.742171619300352 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8336 0.39544535752558224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7312 0.34686857656274683 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6545 0.3104834291032793 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5368 0.25464859395361394 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4508 0.2138516880668576 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4397 0.20858604091170652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4294 0.20369989985782752 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4104 0.1946866299526139 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3981 0.18885172364555458 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3255 0.1544115449551068 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3198 0.1517075639835427 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2818 0.1336810241731155 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2451 0.11627118177725553 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2409 0.11427877474557674 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2380 0.1129030651284652 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2224 0.10550269615365823 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2143 0.10166019687827768 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.743826265901899E-5 0.0 4 0.0 0.0 0.0 4.743826265901899E-5 0.0 5 0.0 0.0 0.0 4.743826265901899E-5 0.0 6 0.0 0.0 0.0 4.743826265901899E-5 0.0 7 0.0 0.0 0.0 4.743826265901899E-5 0.0 8 0.0 0.0 0.0 4.743826265901899E-5 0.0 9 0.0 0.0 0.0 4.743826265901899E-5 0.0 10-11 0.0 0.0 0.0 1.4231478797705697E-4 0.0 12-13 0.0 0.0 0.0 1.4231478797705697E-4 2.3719131329509494E-5 14-15 0.0 0.0 0.0 1.4231478797705697E-4 4.743826265901899E-5 16-17 0.0 0.0 0.0 2.3719131329509493E-4 4.743826265901899E-5 18-19 0.0 0.0 0.0 2.3719131329509493E-4 4.743826265901899E-5 20-21 0.0 0.0 0.0 2.6091044462460444E-4 4.743826265901899E-5 22-23 0.0 0.0 0.0 4.0322523260166135E-4 4.743826265901899E-5 24-25 0.0 0.0 0.0 6.878548085557753E-4 4.743826265901899E-5 26-27 0.0 0.0 0.0 9.250461218508702E-4 4.743826265901899E-5 28-29 0.0 0.0 0.0 0.0013519904857820413 4.743826265901899E-5 30-31 0.0 0.0 0.0 0.0023956322642804586 4.743826265901899E-5 32-33 0.0 0.0 0.0 0.004008533194687105 4.743826265901899E-5 34-35 0.0 0.0 0.0 0.0059060637010478634 4.743826265901899E-5 36-37 0.0 0.0 0.0 0.008301695965328322 4.743826265901899E-5 38-39 0.0 0.0 0.0 0.012666016129958069 4.743826265901899E-5 40-41 0.0 0.0 0.0 0.017694471971814084 4.743826265901899E-5 42-43 0.0 0.0 0.0 0.022224826055750393 4.743826265901899E-5 44-45 0.0 0.0 0.0 0.026328235775755538 4.743826265901899E-5 46-47 0.0 0.0 0.0 0.03235289513345095 4.743826265901899E-5 48-49 0.0 0.0 0.0 0.037144159662011865 4.743826265901899E-5 50-51 0.0 0.0 0.0 0.04108153546271044 9.487652531803798E-5 52-53 0.0 0.0 0.0 0.04981017579196993 1.8975305063607595E-4 54-55 0.0 0.0 0.0 0.06681679295522824 1.8975305063607595E-4 56-57 0.0 0.0 0.0 0.09354825396358543 1.8975305063607595E-4 58-59 0.0 0.0 0.0 0.12632809346096757 1.8975305063607595E-4 60-61 0.0 0.0 0.0 0.16615251496321398 1.8975305063607595E-4 62-63 0.0 0.0 0.0 0.19172173853642524 1.8975305063607595E-4 64-65 0.0 0.0 0.0 0.2219161927188908 1.8975305063607595E-4 66-67 0.0 0.0 0.0 0.2812140210426646 1.8975305063607595E-4 68-69 0.0 0.0 0.0 0.36337709196808543 1.8975305063607595E-4 70-71 0.0 0.0 0.0 0.44516065679223416 2.3719131329509493E-4 72-73 0.0 0.0 0.0 0.5399897438476131 2.3719131329509493E-4 74-75 0.0 0.0 0.0 0.6268966410389358 2.3719131329509493E-4 76-77 0.0 0.0 0.0 0.7057390335782254 2.3719131329509493E-4 78-79 0.0 0.0 0.0 0.7865975522805233 2.6091044462460444E-4 80-81 0.0 0.0 0.0 0.8667444970429359 2.8462957595411394E-4 82-83 0.0 0.0 0.0 0.9673136138800561 3.320678386131329E-4 84-85 0.0 0.0 0.0 1.150591341663176 3.320678386131329E-4 86-87 0.0 0.0 0.0 1.4005198284822176 3.7950610127215185E-4 88-89 0.0 0.0 0.0 1.6907471194300956 4.2694436393117085E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 9270 0.0 23.186052 12-13 TACATGG 10440 0.0 23.071608 2 GTACATG 10830 0.0 22.683216 1 GTACTTT 9560 0.0 22.185122 14-15 ACATGGG 10585 0.0 21.901228 3 AGTACTT 9605 0.0 21.215237 12-13 TACCTGG 1640 0.0 20.567661 2 TAAGGTG 825 0.0 20.150776 5 CATGGGA 5690 0.0 20.03489 4 ACTTTTT 11225 0.0 19.147858 16-17 GTAAGGT 705 0.0 18.865004 4 TACTTTT 10715 0.0 18.818447 14-15 GTGTAGC 1340 0.0 18.793758 1 GTACCTG 1980 0.0 17.518633 1 AGAGTAC 18060 0.0 17.318718 10-11 GTAAGCG 235 0.0 17.17961 94-95 ATGGGAT 2270 0.0 16.948717 5 CATGGGG 4510 0.0 16.851223 4 ATGGGTA 1020 0.0 16.29842 5 AGTACAT 8275 0.0 16.017939 2 >>END_MODULE