FastQCFastQC Report
Thu 26 May 2016
SRR936662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192992
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124030.5655743386204783No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88780.4048350381579139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75500.3442785016999606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60800.2772467934219551No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59950.2733708102902336No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55280.2520757029665407No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38890.17733762822664195No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37080.16908406414615282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35210.16055690125636574No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32770.14943054967824781No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30350.1383953977032292No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28300.12904743838554814No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25760.11746508879193357No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22980.10478834396112709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG107000.025.094061
TACATGG104950.025.040272
ACATGGG108100.023.5627273
GAGTACT76000.021.94423912-13
TAGATCG1100.002869265221.6024685
AGTACTT79850.021.45148312-13
GTACTTT80800.020.69730214-15
TACCTGG16350.020.3458862
CATGGGA58100.019.9576784
CATGGGG49300.019.4715614
GTACCTG18250.019.0093061
GTGTAGC14550.018.6173611
AGAGTAC152900.018.59938410-11
TACTTTT93000.018.56965614-15
TCTATAC5000.018.0580163
ACTTTTT96900.017.47906316-17
TAGCCCT15550.017.4197044
ATGGGAT25400.017.4010455
GACCGTC2755.890579E-717.2788227
AGTACAT79400.016.8781532
Produced by FastQC (version 0.11.5)