##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936662_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2192992 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37192383738746 31.0 31.0 34.0 30.0 34.0 2 31.422938615371145 31.0 31.0 34.0 28.0 34.0 3 31.470370616947076 31.0 31.0 34.0 28.0 34.0 4 35.097283528622086 37.0 35.0 37.0 32.0 37.0 5 35.02284458858035 37.0 35.0 37.0 32.0 37.0 6 35.13965212823394 37.0 35.0 37.0 32.0 37.0 7 34.97667022953116 37.0 35.0 37.0 32.0 37.0 8 34.97531089944697 37.0 35.0 37.0 32.0 37.0 9 36.476257095329125 39.0 37.0 39.0 32.0 39.0 10-11 36.34913670455706 39.0 37.0 39.0 32.0 39.0 12-13 36.26131627475157 39.0 35.0 39.0 32.0 39.0 14-15 37.32187554719762 40.0 37.0 41.0 32.0 41.0 16-17 37.26864644285068 40.0 37.0 41.0 31.5 41.0 18-19 37.24163722439479 39.5 37.0 41.0 31.0 41.0 20-21 37.141646891552725 39.0 36.0 41.0 31.0 41.0 22-23 37.036056219083335 39.0 36.0 41.0 31.0 41.0 24-25 36.87596420780377 39.0 36.0 41.0 30.5 41.0 26-27 36.65783550509988 39.0 36.0 40.5 30.0 41.0 28-29 36.786907567378265 39.0 36.0 41.0 30.0 41.0 30-31 36.7429231387985 39.0 36.0 41.0 30.0 41.0 32-33 36.5902276433293 39.0 35.5 41.0 30.0 41.0 34-35 36.41049192153916 39.0 35.0 40.5 30.0 41.0 36-37 36.16813764026499 39.0 35.0 40.0 29.5 41.0 38-39 35.97423770811749 38.0 35.0 40.0 29.0 41.0 40-41 35.84348164516788 38.0 35.0 40.0 28.5 41.0 42-43 35.57732267149173 38.0 35.0 40.0 27.0 41.0 44-45 35.2968720360129 38.0 34.5 40.0 26.5 41.0 46-47 34.95401282813617 38.0 34.0 40.0 25.5 41.0 48-49 34.683702448526944 38.0 34.0 40.0 24.0 41.0 50-51 33.982637647560956 37.5 33.0 39.5 23.5 40.5 52-53 34.16820353197823 38.0 33.5 39.5 24.0 40.5 54-55 34.52274084903182 38.0 34.0 40.0 24.0 41.0 56-57 34.42698172177555 38.0 34.0 40.0 23.5 41.0 58-59 34.18669060352249 38.0 33.5 40.0 23.0 41.0 60-61 33.83414759378967 37.5 33.0 40.0 22.5 41.0 62-63 33.5253384417271 37.0 33.0 40.0 22.0 41.0 64-65 33.20412819563409 36.0 32.5 39.5 21.5 41.0 66-67 32.800791110957086 36.0 32.0 39.0 20.0 41.0 68-69 32.406583790547344 35.0 31.5 39.0 19.5 40.0 70-71 31.985477831200477 35.0 31.0 38.0 18.0 40.0 72-73 31.57265530380412 35.0 31.0 37.0 17.0 39.5 74-75 31.15077437582992 35.0 31.0 37.0 13.0 39.0 76-77 30.731503808495425 35.0 30.0 36.0 9.5 39.0 78-79 30.279325460375595 34.0 30.0 36.0 7.0 38.5 80-81 29.85243995418132 34.0 29.5 35.5 6.0 37.0 82-83 29.446363233427206 34.0 29.0 35.0 2.0 37.0 84-85 29.051928598006743 34.0 29.0 35.0 2.0 36.0 86-87 28.73499492930207 34.0 29.0 35.0 2.0 36.0 88-89 28.349076056821 34.0 28.5 35.0 2.0 36.0 90-91 27.97839367403073 34.0 27.0 35.0 2.0 35.0 92-93 27.612800229093402 34.0 27.0 35.0 2.0 35.0 94-95 27.188074785498536 33.0 26.0 35.0 2.0 35.0 96-97 26.613295898936247 33.0 25.0 35.0 2.0 35.0 98-99 25.96000122207468 33.0 24.5 35.0 2.0 35.0 100-101 23.851853084735374 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15186566516965527 1101 2 0.24597889788390503 1101 3 0.2512004378156618 1101 4 0.32380015317936284 1101 5 0.34200048346506406 1101 6 0.17305643981715946 1101 7 0.2442984463718716 1101 8 0.17742906340964026 1101 9 0.33352438182955524 1101 10-11 0.27773912787275634 1101 12-13 0.3700832160485845 1101 14-15 0.5219671148204199 1101 16-17 0.5134125318818619 1101 18-19 0.5501728791866398 1101 20-21 0.4828121766300484 1101 22-23 0.37308264399937485 1101 24-25 0.36103956404098625 1101 26-27 0.4133882224557155 1101 28-29 0.4289531406349596 1101 30-31 0.28667876780452417 1101 32-33 0.2673394513712637 1101 34-35 0.3377836756198036 1101 36-37 0.35568875746757556 1101 38-39 0.4772120152076269 1101 40-41 0.42519138388885125 1101 42-43 0.2255937051791932 1101 44-45 0.1707788098260039 1101 46-47 0.24327966086450914 1101 48-49 0.13580425147505792 1101 50-51 0.20174472327270365 1101 52-53 0.10522643024321354 1101 54-55 0.22567432397525522 1101 56-57 0.16338076617738295 1101 58-59 0.10443635960445619 1101 60-61 0.08697002445431679 1101 62-63 0.19188857485157484 1101 64-65 0.3262015723868359 1101 66-67 0.16877370865890384 1101 68-69 0.13923555966395185 1101 70-71 0.09603804880543976 1101 72-73 0.1512519457082675 1101 74-75 0.20093017560033033 1101 76-77 0.09870370088319902 1101 78-79 0.09726896681426922 1101 80-81 0.1228951967593197 1101 82-83 0.008014107170495777 1101 84-85 0.08749531044023584 1101 86-87 0.18968002472390566 1101 88-89 0.08071242465321049 1101 90-91 0.14540332034614778 1101 92-93 -0.08072822591564943 1101 94-95 -0.1305859719143463 1101 96-97 -0.16278911943009788 1101 98-99 -0.23244323660209787 1101 100-101 0.18098423377426798 1102 1 0.13142675023451034 1102 2 0.1832583213081378 1102 3 0.25019639780000347 1102 4 0.2628802532606116 1102 5 0.236578106072308 1102 6 0.15339858522123961 1102 7 -0.06158111653744669 1102 8 -0.020620390647984266 1102 9 0.4089670084150754 1102 10-11 0.41917524889641555 1102 12-13 0.3629567771701332 1102 14-15 0.27482361217314377 1102 16-17 0.1294879071514643 1102 18-19 0.141680496840344 1102 20-21 -0.15345429601109117 1102 22-23 -0.3048017433537922 1102 24-25 -0.0903855272768368 1102 26-27 -0.05902579565489674 1102 28-29 0.05420920241253668 1102 30-31 -0.0157110017924893 1102 32-33 -0.09077053977410543 1102 34-35 -0.03177714282366395 1102 36-37 -0.11880792449941424 1102 38-39 -0.12053650749939493 1102 40-41 -0.3446556315653595 1102 42-43 -0.28299662544536375 1102 44-45 0.015119700706293315 1102 46-47 0.03115568472571084 1102 48-49 0.01959612681974221 1102 50-51 -0.1340777545942089 1102 52-53 -0.06933753854243463 1102 54-55 -0.16621421144539283 1102 56-57 -0.04649734575026798 1102 58-59 -0.08484391700820737 1102 60-61 -0.41147459056544733 1102 62-63 -0.26428125455284146 1102 64-65 -0.07766703378671025 1102 66-67 -0.017072835296438882 1102 68-69 -0.06718201714054572 1102 70-71 0.04416334963154611 1102 72-73 -0.244023803953894 1102 74-75 -0.21679348115410235 1102 76-77 0.006976120584553769 1102 78-79 -0.10016045218966241 1102 80-81 -0.05374105168129617 1102 82-83 -0.06346673608279119 1102 84-85 -0.26154309847558466 1102 86-87 -0.2312294067971976 1102 88-89 -0.31069752537632667 1102 90-91 -0.41817381251970787 1102 92-93 -0.5425421494458291 1102 94-95 -0.4104989972111035 1102 96-97 -0.4751181133834024 1102 98-99 -0.3486866535095423 1102 100-101 0.09672128053544782 1103 1 0.11951441371984117 1103 2 0.21195832402722914 1103 3 0.2003003672431447 1103 4 0.15258734994432643 1103 5 0.34261777903724777 1103 6 -0.002729028862503924 1103 7 0.24070535255150105 1103 8 -0.09300458958239233 1103 9 0.2266441035102602 1103 10-11 0.25785259729637033 1103 12-13 0.3017476833858339 1103 14-15 0.30592564533746724 1103 16-17 0.3456112565560545 1103 18-19 0.28365137967186627 1103 20-21 0.2526101349905474 1103 22-23 0.13885862504007207 1103 24-25 0.2280414644213522 1103 26-27 0.3210865600365196 1103 28-29 0.27866434671754803 1103 30-31 0.16568655360182305 1103 32-33 0.24526628530106365 1103 34-35 0.05393596734387529 1103 36-37 0.2877274816397133 1103 38-39 0.20608775566279292 1103 40-41 0.01391079224357128 1103 42-43 0.25155149745181404 1103 44-45 0.3670353001298494 1103 46-47 0.37553067309802657 1103 48-49 0.368530672581322 1103 50-51 -0.13526599724586674 1103 52-53 0.06144836454630109 1103 54-55 0.32091912274660217 1103 56-57 0.3469807004542389 1103 58-59 0.24949268925170287 1103 60-61 0.48573454901406876 1103 62-63 0.3303106886382068 1103 64-65 0.4233025367257568 1103 66-67 0.36495075496235074 1103 68-69 0.26425260375380333 1103 70-71 0.37058267921825205 1103 72-73 0.21287524479367903 1103 74-75 0.1503477384010239 1103 76-77 0.05678878954384459 1103 78-79 0.24996119134326733 1103 80-81 0.5651677915173288 1103 82-83 0.5237012307385776 1103 84-85 0.22482717298984767 1103 86-87 0.3261479872548634 1103 88-89 0.31926692609004803 1103 90-91 0.29535166035163485 1103 92-93 0.204182634207573 1103 94-95 0.10857213277145306 1103 96-97 0.2073247892888901 1103 98-99 0.04226170809517882 1103 100-101 0.0549498215429125 1104 1 0.30712195288525024 1104 2 0.33560368313047206 1104 3 0.3274101390663766 1104 4 0.33920126062435685 1104 5 0.4196867101020274 1104 6 0.31687551501703837 1104 7 0.5141938362295946 1104 8 0.4899938970381399 1104 9 0.5477336714058296 1104 10-11 0.5201751196311122 1104 12-13 0.47898180351615594 1104 14-15 0.508108258011795 1104 16-17 0.3617232401045527 1104 18-19 0.43753151894909337 1104 20-21 0.4839468288161015 1104 22-23 0.6325654219458556 1104 24-25 0.5133458579724604 1104 26-27 0.4601097043206366 1104 28-29 0.5194889084179692 1104 30-31 0.42993739513522655 1104 32-33 0.34873176691767327 1104 34-35 0.40132521703485935 1104 36-37 0.4092533078151561 1104 38-39 0.43932522975318733 1104 40-41 0.3165913622861396 1104 42-43 0.30905509235899586 1104 44-45 0.21209146025531567 1104 46-47 0.42242019877217984 1104 48-49 0.25500590877036444 1104 50-51 0.2779946748703921 1104 52-53 0.4001590644093369 1104 54-55 0.5867871900241397 1104 56-57 0.6331639645616605 1104 58-59 0.5366904401744463 1104 60-61 0.5760810318542724 1104 62-63 0.40932196816981303 1104 64-65 0.43703013851663286 1104 66-67 0.46819807774745215 1104 68-69 0.4902022812884752 1104 70-71 0.41086709182541625 1104 72-73 0.10761037963350262 1104 74-75 -0.1042046477770775 1104 76-77 0.03526915406134634 1104 78-79 0.30107991042505944 1104 80-81 0.4822767883905108 1104 82-83 0.24184365765054494 1104 84-85 0.3114400890021862 1104 86-87 0.2940231900111918 1104 88-89 0.2507833875434109 1104 90-91 0.42486912181717074 1104 92-93 0.3103196893201776 1104 94-95 0.2628372232906635 1104 96-97 0.41811305897982365 1104 98-99 0.49305245406328524 1104 100-101 0.7211260576550487 1105 1 0.21072303816315596 1105 2 0.2134146035395048 1105 3 0.15060208329060742 1105 4 0.18865257824121073 1105 5 0.26133769076038504 1105 6 0.1832975076498542 1105 7 0.22571493564176137 1105 8 -0.03907171330055803 1105 9 0.3503243358022772 1105 10-11 0.32050133948313686 1105 12-13 0.052077922912403096 1105 14-15 0.08388817805037974 1105 16-17 0.12282798553815866 1105 18-19 0.04503796174805785 1105 20-21 -0.2288125860418404 1105 22-23 -0.39555840081968086 1105 24-25 -0.2281958079168973 1105 26-27 -0.27329551265734153 1105 28-29 0.056966488543594096 1105 30-31 -0.04969428322821301 1105 32-33 -0.22075099221657268 1105 34-35 -0.34646924423521597 1105 36-37 -0.496189730536571 1105 38-39 -0.3047481712028244 1105 40-41 -0.3178469913484676 1105 42-43 -0.19111131754677047 1105 44-45 -0.0571856341070216 1105 46-47 -0.03647984664369375 1105 48-49 0.1589761057987502 1105 50-51 0.09463063317432585 1105 52-53 0.07161213877551376 1105 54-55 0.041795375698399084 1105 56-57 0.16109378614171277 1105 58-59 -0.024439999638389054 1105 60-61 0.11450008601579498 1105 62-63 -0.0600819676948845 1105 64-65 0.16753693859131857 1105 66-67 -0.12964122719058224 1105 68-69 -0.001751725302391094 1105 70-71 0.012671153920216227 1105 72-73 0.08957540235633132 1105 74-75 -0.082468363033815 1105 76-77 0.0729859631235783 1105 78-79 0.019382193901314793 1105 80-81 -0.012223611134356105 1105 82-83 0.1108994417312239 1105 84-85 -0.05855497435350543 1105 86-87 -0.07184117658787414 1105 88-89 0.018331088711665444 1105 90-91 -0.07000430867404717 1105 92-93 0.006924958596176367 1105 94-95 -0.013878072483933579 1105 96-97 0.1527278243591006 1105 98-99 -0.28100200767395833 1105 100-101 -0.05690484050224498 1106 1 0.05018768467929746 1106 2 0.11120616845944298 1106 3 -0.008876123791814905 1106 4 0.18001833798128786 1106 5 0.35845649525542456 1106 6 0.2693523854603157 1106 7 0.3914908192451989 1106 8 0.4957719748336018 1106 9 0.4804816417697779 1106 10-11 0.4118407597774265 1106 12-13 0.42263569041496396 1106 14-15 0.3429338234602639 1106 16-17 0.36217605876521475 1106 18-19 0.35016404272740687 1106 20-21 0.3913379396425185 1106 22-23 0.3229272169932642 1106 24-25 0.06692085883474164 1106 26-27 0.3549287328874655 1106 28-29 0.1791852554836808 1106 30-31 0.19783227288075977 1106 32-33 0.17829161141215621 1106 34-35 0.1812955054295955 1106 36-37 0.3195550555276512 1106 38-39 0.171776330429374 1106 40-41 0.17208505288140685 1106 42-43 0.19164892042586956 1106 44-45 0.08630699339713743 1106 46-47 0.24689613959861845 1106 48-49 0.22620864571999988 1106 50-51 0.40123189304949136 1106 52-53 0.1846570466809183 1106 54-55 0.5197710958273518 1106 56-57 0.44979186981958463 1106 58-59 0.4180411931476584 1106 60-61 0.41571407956858053 1106 62-63 0.4070995926538856 1106 64-65 0.31815234699716655 1106 66-67 0.41519020885299085 1106 68-69 0.320212398673128 1106 70-71 0.28167769799800624 1106 72-73 0.5179607433501943 1106 74-75 0.38630299528290735 1106 76-77 0.2324113213825214 1106 78-79 0.4302545223593057 1106 80-81 0.3986512663022559 1106 82-83 0.586170695539824 1106 84-85 0.42751137386076365 1106 86-87 0.565864504745619 1106 88-89 0.6758514253127892 1106 90-91 0.8057572836559075 1106 92-93 0.6595592430383981 1106 94-95 0.6942927634621014 1106 96-97 0.7958392096270224 1106 98-99 0.9309291665045478 1106 100-101 1.0910417023180479 1107 1 0.465140090547564 1107 2 0.4375637248244466 1107 3 0.44675903482174917 1107 4 0.48068311295730126 1107 5 0.5596210253849563 1107 6 0.42551480253114704 1107 7 0.5729193810907418 1107 8 0.6812851126922368 1107 9 0.7129019995810566 1107 10-11 0.7555260683175291 1107 12-13 0.789345289067974 1107 14-15 0.8689811291427176 1107 16-17 0.8998441810945437 1107 18-19 0.9738883254613739 1107 20-21 0.9835088894456376 1107 22-23 0.950000194661925 1107 24-25 0.8614121483371946 1107 26-27 0.9601171305181566 1107 28-29 0.8070917174184373 1107 30-31 0.693132562000855 1107 32-33 0.9405255504889922 1107 34-35 0.822372360393274 1107 36-37 0.8427353094971863 1107 38-39 0.8899275046862769 1107 40-41 0.7737171391746998 1107 42-43 0.8861381974914622 1107 44-45 0.8562636589234174 1107 46-47 1.2269820706525323 1107 48-49 1.2027422903102334 1107 50-51 1.113276663061498 1107 52-53 1.2419447513625173 1107 54-55 1.4573248446618123 1107 56-57 1.407954313722911 1107 58-59 1.4650949855849191 1107 60-61 1.3616090437807458 1107 62-63 1.5191527478194757 1107 64-65 1.3217653419727853 1107 66-67 1.2606786608194795 1107 68-69 1.4218159754442468 1107 70-71 1.573741653241413 1107 72-73 1.6352318118870812 1107 74-75 1.2341221845391779 1107 76-77 1.4568677957273053 1107 78-79 1.463983970347872 1107 80-81 1.555273525195549 1107 82-83 1.4725548831526005 1107 84-85 1.5783713552121803 1107 86-87 1.6611808933605943 1107 88-89 1.8154109028841816 1107 90-91 1.6988204138001954 1107 92-93 1.6992139759544855 1107 94-95 1.7846243459753701 1107 96-97 1.958197919789157 1107 98-99 2.045969784188504 1107 100-101 2.054809453688179 1108 1 0.27565290982133206 1108 2 0.1932807327316901 1108 3 0.03989588703244351 1108 4 0.21882023094423175 1108 5 0.30561384459429775 1108 6 0.2698859034729608 1108 7 0.4081324772550232 1108 8 0.2210935605106954 1108 9 0.5542392245227816 1108 10-11 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-2.8706690961929553 2316 84-85 -3.0687992874934444 2316 86-87 -3.047929085043176 2316 88-89 -3.355963978469397 2316 90-91 -3.5564790032639273 2316 92-93 -3.78761645598491 2316 94-95 -3.9287635462589954 2316 96-97 -3.967623526759798 2316 98-99 -4.424289267260129 2316 100-101 -4.852730849190479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4986.0 3 1991.0 4 1873.0 5 2179.0 6 2471.0 7 3026.0 8 3658.0 9 4533.0 10 5802.0 11 7209.0 12 8863.0 13 10665.0 14 12138.0 15 14456.0 16 17571.0 17 21211.0 18 18798.0 19 13972.0 20 14464.0 21 15689.0 22 17211.0 23 19380.0 24 22383.0 25 25687.0 26 30163.0 27 35473.0 28 42529.0 29 51278.0 30 61375.0 31 76521.0 32 95083.0 33 121500.0 34 159822.0 35 217542.0 36 301174.0 37 384957.0 38 299162.0 39 46178.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15196029025257 18.09011766712531 14.376130734966896 22.381791307655227 2 15.482530844352024 19.97231931742264 40.09011042631443 24.455039411910903 3 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23.315067106381584 26.40460095506663 27.89153445426588 22.38879748428591 48-49 23.064807491360497 26.33556310033906 27.854295947590934 22.745333460709503 50-51 22.405199746643 26.7778880805927 28.276825814324337 22.540086358439968 52-53 22.918326804502854 27.063604055786563 27.320128839236187 22.697940300474404 54-55 22.42652717030338 26.51994255191612 27.768337113272544 23.285193164507955 56-57 22.97939315616298 26.6122388860781 27.298601669496588 23.10976628826234 58-59 23.61103194294785 26.252370674815236 27.299389267654718 22.837208114582197 60-61 22.934233176392798 26.438217489939465 28.26888131419647 22.358668019471267 62-63 22.58961082192749 26.622525314016933 28.117448830459356 22.67041503359622 64-65 22.419683535962797 27.225880208069704 27.584944997968464 22.769491257999043 66-67 22.596872500494772 26.776341650045097 27.14755665241179 23.479229197048337 68-69 22.50524545443352 26.74199022186186 27.57034107652549 23.18242324717913 70-71 23.072509980508705 26.542589432381085 27.196418782783816 23.18848180432639 72-73 22.76915577205193 26.318400768296634 27.9231229105893 22.989320549062136 74-75 22.4031731186 27.4943722335706 27.198556811085123 22.90389783674427 76-77 22.708011417619332 26.659511844104216 27.175238203378 23.457238534898455 78-79 22.690056745611795 26.82443528080867 27.43096102616502 23.05454694741452 80-81 23.13933186529301 26.79501225089989 27.071341651417658 22.994314232389446 82-83 22.890196290345273 26.759434255879032 27.55967612742855 22.79069332634715 84-85 22.75618231140567 27.04666184940454 27.188033063534732 23.009122775655054 86-87 22.7417165148003 27.210536900539807 27.09155806636821 22.956188518291682 88-89 22.701785107610366 27.04569136722963 26.6467612005299 23.60576232463011 90-91 22.65386550986823 26.556692069684274 27.147465577546775 23.641976842900718 92-93 23.041685137247818 26.889403632821264 27.272633986963875 22.79627724296704 94-95 22.70321012358521 26.67309510709647 27.423644788916025 23.2000499804023 96-97 22.096222266202503 26.70114761170358 27.883530590970324 23.319099531123598 98-99 22.296016673192696 27.152296132978698 26.905796871746723 23.64589032208188 100-101 22.230831518082184 26.61674440883638 27.363525426821617 23.788898646259824 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 219.0 1 165.5 2 191.5 3 275.0 4 245.0 5 263.0 6 410.0 7 817.0 8 1476.0 9 1831.5 10 1688.5 11 1604.5 12 1660.0 13 1752.5 14 1950.5 15 2133.5 16 2299.0 17 2500.0 18 2772.5 19 3032.0 20 3338.0 21 3882.5 22 4695.0 23 5594.0 24 6730.0 25 8308.0 26 10661.5 27 13488.0 28 16001.0 29 19208.0 30 23316.5 31 27576.0 32 32450.5 33 36177.5 34 39341.5 35 42850.5 36 47767.5 37 52451.0 38 54125.0 39 55898.0 40 58156.0 41 60573.0 42 62647.0 43 65171.0 44 68924.0 45 73529.5 46 78047.5 47 90197.0 48 115234.5 49 114434.5 50 90205.0 51 79191.0 52 76319.0 53 75006.0 54 73672.0 55 69861.0 56 63511.0 57 56954.5 58 52435.5 59 47056.5 60 39726.5 61 33731.5 62 27932.5 63 21646.0 64 15544.5 65 11450.5 66 8995.5 67 7202.5 68 5695.5 69 4532.0 70 3636.0 71 2619.5 72 1998.5 73 1735.5 74 1293.5 75 968.5 76 729.0 77 442.5 78 288.5 79 205.0 80 148.0 81 85.5 82 57.5 83 43.0 84 31.5 85 23.0 86 14.5 87 11.0 88 10.0 89 7.0 90 6.5 91 6.0 92 4.0 93 2.0 94 1.5 95 2.0 96 1.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012813544235455488 2 0.005791174796807284 3 0.008709562095985758 4 0.03483824838394303 5 0.04632939837445827 6 0.056634953524682265 7 0.024532693233719045 8 0.024122295019772075 9 0.013406341655601114 10-11 0.04382140928922677 12-13 0.03969462724898221 14-15 0.017989121711342315 16-17 0.021842304942288893 18-19 0.028522675869314614 20-21 0.023301498591878127 22-23 0.013406341655601114 24-25 0.0031463863069267923 26-27 0.002530788986006333 28-29 0.0014363937488144052 30-31 0.006703170827800557 32-33 0.001732792458887219 34-35 0.0018011921612117144 36-37 0.005882374399906611 38-39 0.00624717281230392 40-41 0.004058382337920065 42-43 9.803957333177687E-4 44-45 0.0014363937488144052 46-47 0.0025535888867811644 48-49 5.699975193707956E-4 50-51 0.0017783922604368827 52-53 0.0012083947410660868 54-55 0.002530788986006333 56-57 0.002530788986006333 58-59 0.0023255898790328464 60-61 7.751966263442821E-4 62-63 0.0016871926573375554 64-65 0.003328785513125447 66-67 0.002644788489880492 68-69 0.006429572018502576 70-71 0.009940756737826677 72-73 0.004058382337920065 74-75 0.003214786009251288 76-77 0.0029867870015029695 78-79 0.03429105076534707 80-81 0.02279990077483183 82-83 0.0042179816433438886 84-85 0.008367563584363281 86-87 0.007523967255694503 88-89 0.005791174796807284 90-91 0.012471545723833011 92-93 0.014409537289693715 94-95 0.006041973705330435 96-97 0.004103982139469729 98-99 0.022366702660110024 100-101 0.041815018021041574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2192992.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.123442053045224 #Duplication Level Percentage of deduplicated Percentage of total 1 79.00730135942362 45.92176302331941 2 12.526465151043878 14.561625426723785 3 3.794699028298122 6.6168290724009875 4 1.6060801531977056 3.734036268677314 5 0.8120355500258114 2.3599150618468974 6 0.4846107009537164 1.6900345197101379 7 0.33235838958875485 1.3522469518673796 8 0.2182792619049179 1.014971362456958 9 0.17505082551285303 0.9157100861730639 >10 0.9298292615637527 10.007863546199253 >50 0.07233804555739035 2.8760483902398266 >100 0.036317508340166296 3.81984546084921 >500 0.002435215292720748 0.8897581585006102 >1k 0.0017282173045114085 2.1192197448886714 >5k 3.9277666011622916E-4 1.553823569145223 >10k+ 7.855533202324582E-5 0.5663093570012107 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12403 0.5655743386204783 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8878 0.4048350381579139 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7550 0.3442785016999606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6080 0.2772467934219551 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5995 0.2733708102902336 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5528 0.2520757029665407 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3889 0.17733762822664195 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3708 0.16908406414615282 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3521 0.16055690125636574 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3277 0.14943054967824781 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3035 0.1383953977032292 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2830 0.12904743838554814 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2576 0.11746508879193357 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2298 0.10478834396112709 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.559980154966365E-5 0.0 3 0.0 0.0 0.0 4.559980154966365E-5 0.0 4 0.0 0.0 0.0 4.559980154966365E-5 0.0 5 0.0 0.0 0.0 4.559980154966365E-5 0.0 6 0.0 0.0 0.0 4.559980154966365E-5 4.559980154966365E-5 7 0.0 0.0 0.0 4.559980154966365E-5 4.559980154966365E-5 8 0.0 0.0 0.0 4.559980154966365E-5 4.559980154966365E-5 9 0.0 0.0 0.0 4.559980154966365E-5 4.559980154966365E-5 10-11 0.0 0.0 0.0 9.11996030993273E-5 4.559980154966365E-5 12-13 0.0 0.0 0.0 9.11996030993273E-5 1.8239920619865464E-4 14-15 0.0 0.0 0.0 1.595993054238228E-4 2.279990077483183E-4 16-17 0.0 0.0 0.0 1.823992061986546E-4 2.7359880929798193E-4 18-19 0.0 0.0 0.0 2.279990077483183E-4 2.7359880929798193E-4 20-21 0.0 0.0 0.0 2.507989085231501E-4 2.9639871007281377E-4 22-23 0.0 0.0 0.0 3.191986108476456E-4 3.647984123973092E-4 24-25 0.0 0.0 0.0 5.015978170463002E-4 3.647984123973092E-4 26-27 0.0 0.0 0.0 6.155973209204593E-4 4.103982139469729E-4 28-29 0.0 0.0 0.0 0.001185594840291255 4.103982139469729E-4 30-31 0.0 0.0 0.0 0.0020063912681852008 4.103982139469729E-4 32-33 0.0 0.0 0.0 0.0031463863069267923 4.103982139469729E-4 34-35 0.0 0.0 0.0 0.0046967795596153565 4.103982139469729E-4 36-37 0.0 4.559980154966365E-5 0.0 0.007250368446396521 4.103982139469729E-4 38-39 0.0 4.559980154966365E-5 0.0 0.011947148006011876 4.559980154966366E-4 40-41 0.0 4.559980154966365E-5 0.0 0.01607393004625644 4.559980154966366E-4 42-43 0.0 4.559980154966365E-5 0.0 0.019904313376428187 4.559980154966366E-4 44-45 0.0 4.559980154966365E-5 0.0 0.02332429849265296 4.559980154966366E-4 46-47 0.0 4.559980154966365E-5 0.0 0.02827187696079147 4.787979162714684E-4 48-49 0.0 4.559980154966365E-5 0.0 0.03173746187856591 5.471976185959639E-4 50-51 0.0 4.559980154966365E-5 0.0 0.03575024441493631 5.927974201456275E-4 52-53 0.0 4.559980154966365E-5 0.0 0.04430020720549824 5.927974201456275E-4 54-55 0.0 4.559980154966365E-5 0.0 0.05918854241146343 5.927974201456275E-4 56-57 0.0 4.559980154966365E-5 0.0 0.08226204199559323 5.927974201456275E-4 58-59 0.0 4.559980154966365E-5 0.0 0.1129051086369672 5.927974201456275E-4 60-61 0.0 4.559980154966365E-5 0.0 0.1484273540441552 5.927974201456275E-4 62-63 0.0 4.559980154966365E-5 0.0 0.17011005968102028 6.383972216952912E-4 64-65 0.0 4.559980154966365E-5 0.0 0.19753834031314296 6.383972216952912E-4 66-67 0.0 4.559980154966365E-5 0.0 0.25171090455414336 6.611971224701231E-4 68-69 0.0 4.559980154966365E-5 0.0 0.3276573740351082 6.839970232449549E-4 70-71 0.0 4.559980154966365E-5 0.0 0.40255504808043074 6.839970232449549E-4 72-73 0.0 4.559980154966365E-5 0.0 0.48680068144343436 6.839970232449549E-4 74-75 0.0 4.559980154966365E-5 0.0 0.5640923450701143 6.839970232449549E-4 76-77 0.0 4.559980154966365E-5 0.0 0.632058849279888 6.839970232449549E-4 78-79 0.0 4.559980154966365E-5 0.0 0.7043345347361049 6.839970232449549E-4 80-81 0.0 4.559980154966365E-5 0.0 0.7754246253520305 6.839970232449549E-4 82-83 0.0 4.559980154966365E-5 0.0 0.8733273992791584 6.839970232449549E-4 84-85 0.0 4.559980154966365E-5 0.0 1.0497074316732573 6.839970232449549E-4 86-87 0.0 4.559980154966365E-5 0.0 1.2903603843516074 7.295968247946186E-4 88-89 0.0 4.559980154966365E-5 0.0 1.5699783674541448 7.751966263442821E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10700 0.0 25.09406 1 TACATGG 10495 0.0 25.04027 2 ACATGGG 10810 0.0 23.562727 3 GAGTACT 7600 0.0 21.944239 12-13 TAGATCG 110 0.0028692652 21.602468 5 AGTACTT 7985 0.0 21.451483 12-13 GTACTTT 8080 0.0 20.697302 14-15 TACCTGG 1635 0.0 20.345886 2 CATGGGA 5810 0.0 19.957678 4 CATGGGG 4930 0.0 19.471561 4 GTACCTG 1825 0.0 19.009306 1 GTGTAGC 1455 0.0 18.617361 1 AGAGTAC 15290 0.0 18.599384 10-11 TACTTTT 9300 0.0 18.569656 14-15 TCTATAC 500 0.0 18.058016 3 ACTTTTT 9690 0.0 17.479063 16-17 TAGCCCT 1555 0.0 17.419704 4 ATGGGAT 2540 0.0 17.401045 5 GACCGTC 275 5.890579E-7 17.278822 7 AGTACAT 7940 0.0 16.878153 2 >>END_MODULE