FastQCFastQC Report
Thu 26 May 2016
SRR936662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192992
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT155370.7084841166771242No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86430.394119084793743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73770.3363897360318688No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66060.3012322890370781No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53780.24523573273409113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45500.20747909705096965No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45390.20697749923392333No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45090.20560950518743343No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC41220.1879623819877136No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39600.18057521413666808No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32690.14906575126585048No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG32220.1469225605930163No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26680.12166027053450264No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24420.11135471538427864No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.10364834892238549No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22650.10328355050998818No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22400.10214355547124658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG103550.023.16632
GTACATG107600.023.017761
GAGTACT96100.021.76802612-13
ACATGGG106800.021.7052083
AGTACTT99600.021.3606912-13
GTACTTT100950.020.72221414-15
TAAGGTG9650.020.1782195
GTACCTG17400.019.3096491
TACTTTT112400.018.92815414-15
TATACTG9650.018.7017635
GTAAGGT8700.018.5620334
GTATAGC8350.018.3945471
TACCTGG17050.018.388022
ACTTTTT116550.018.25480516-17
CATGGGA58850.018.1594184
GTGTAGC12350.017.87791
CATGGGG45150.017.7784884
AGAGTAC184450.017.6363410-11
GTATAGG5700.016.84151
CTAGACT13700.016.294584
Produced by FastQC (version 0.11.5)