##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936662_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2192992 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.545109603683006 31.0 31.0 34.0 30.0 34.0 2 31.75051345376545 31.0 31.0 34.0 30.0 34.0 3 32.03271512162379 33.0 31.0 34.0 30.0 34.0 4 35.81358846726299 37.0 35.0 37.0 35.0 37.0 5 35.64008213436255 37.0 35.0 37.0 33.0 37.0 6 35.75210032685938 37.0 35.0 37.0 33.0 37.0 7 35.6920285162919 37.0 35.0 37.0 33.0 37.0 8 35.67439507303264 37.0 35.0 37.0 33.0 37.0 9 37.31806773576921 39.0 37.0 39.0 34.0 39.0 10-11 37.2051728870876 39.0 37.0 39.0 33.0 39.0 12-13 37.080658068976085 39.0 37.0 39.0 33.0 39.0 14-15 38.19453376938904 40.0 37.5 41.0 33.0 41.0 16-17 38.12395758853657 40.0 37.0 41.0 33.0 41.0 18-19 38.20109261684493 40.0 37.5 41.0 33.5 41.0 20-21 38.27486192380091 40.0 38.0 41.0 34.0 41.0 22-23 38.18908140111775 40.0 37.5 41.0 33.0 41.0 24-25 38.08147590141688 40.0 37.0 41.0 33.0 41.0 26-27 37.868056974216046 40.0 37.0 41.0 33.0 41.0 28-29 37.8002913371321 40.0 37.0 41.0 33.0 41.0 30-31 37.80645825429367 40.0 37.0 41.0 33.0 41.0 32-33 37.63380463768222 40.0 36.5 41.0 33.0 41.0 34-35 37.45909925800002 40.0 36.0 41.0 32.0 41.0 36-37 37.22972108425384 39.0 36.0 41.0 31.5 41.0 38-39 37.06694848864018 39.0 36.0 41.0 31.0 41.0 40-41 36.85333918226788 39.0 35.5 41.0 31.0 41.0 42-43 36.611783581517855 39.0 35.0 41.0 30.0 41.0 44-45 36.33345538880215 39.0 35.0 40.0 30.0 41.0 46-47 35.939296860180065 39.0 35.0 40.0 29.5 41.0 48-49 35.47846572171718 38.0 35.0 40.0 27.5 41.0 50-51 35.27734027301513 38.0 34.5 40.0 27.0 41.0 52-53 35.0146099484175 38.0 34.0 40.0 26.5 41.0 54-55 34.75696172170259 38.0 34.0 40.0 26.0 41.0 56-57 34.45100141724183 38.0 34.0 40.0 25.5 41.0 58-59 34.08844970706687 37.5 33.0 40.0 24.5 41.0 60-61 33.683296154295135 37.0 33.0 39.5 23.0 41.0 62-63 33.38616260342035 36.0 32.0 39.0 23.0 40.5 64-65 32.95172098211029 36.0 32.0 39.0 22.0 40.0 66-67 32.53238452306256 35.0 31.0 38.5 21.5 40.0 68-69 32.06346717179087 35.0 31.0 38.0 20.0 40.0 70-71 31.57165826414323 35.0 31.0 37.5 18.5 39.5 72-73 31.150314729830296 34.0 30.0 37.0 18.0 39.0 74-75 30.775795807736646 34.0 30.0 36.0 17.0 39.0 76-77 29.36391651223534 32.5 28.5 35.0 13.0 37.0 78-79 30.16712509667158 34.0 30.0 35.0 13.5 37.0 80-81 30.00473075141177 34.0 30.0 35.0 9.5 37.0 82-83 29.66332184522333 34.0 29.5 35.0 7.0 36.5 84-85 29.214232427660477 34.0 29.0 35.0 4.5 36.0 86-87 28.858748914724725 34.0 29.0 35.0 2.0 36.0 88-89 28.439093485065154 33.0 28.5 35.0 2.0 35.0 90-91 27.97113897360319 33.0 27.0 35.0 2.0 35.0 92-93 27.516365768776176 33.0 26.5 35.0 2.0 35.0 94-95 26.979338273919833 33.0 25.5 34.0 2.0 35.0 96-97 26.287275785775783 32.0 25.0 34.0 2.0 35.0 98-99 25.437907662225854 32.0 22.0 34.0 2.0 35.0 100-101 22.59839570778188 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3197755501340467 1101 2 0.3105185750499153 1101 3 0.13890552047050164 1101 4 0.10337751292586006 1101 5 0.13897606383691397 1101 6 0.10829523943376529 1101 7 0.10649381484222431 1101 8 0.07178262635502364 1101 9 0.09968738466197635 1101 10-11 0.11482835300723337 1101 12-13 0.1025078536127566 1101 14-15 0.18235977992022612 1101 16-17 0.1747856511916268 1101 18-19 0.11771978673810679 1101 20-21 -0.0068394194525964735 1101 22-23 -0.10453446845355785 1101 24-25 -0.16978901069661845 1101 26-27 -0.1059531647729628 1101 28-29 -0.04092733337053289 1101 30-31 -0.10221208287499195 1101 32-33 -0.040276120810723626 1101 34-35 0.17246731009813487 1101 36-37 0.18917478821619937 1101 38-39 0.1398654622022093 1101 40-41 0.22034757834830287 1101 42-43 0.23134519521453 1101 44-45 0.18895715521812662 1101 46-47 0.3055083279038655 1101 48-49 0.34502823999014254 1101 50-51 0.2577532911791067 1101 52-53 0.3170333809651211 1101 54-55 0.3570398452753807 1101 56-57 0.1999700679533447 1101 58-59 0.19029426024343365 1101 60-61 0.25047657823083824 1101 62-63 0.08955517041560768 1101 64-65 0.2628849999038394 1101 66-67 0.025597502443332587 1101 68-69 -0.14667346744201737 1101 70-71 -0.07324966185386828 1101 72-73 -0.05353107482680031 1101 74-75 -0.009981091897731886 1101 76-77 0.37787458769439297 1101 78-79 0.2735745603397426 1101 80-81 0.1116952836521179 1101 82-83 0.10656144749719232 1101 84-85 0.3068092664664199 1101 86-87 0.1806044226548167 1101 88-89 0.08703256735347153 1101 90-91 0.004452528384529586 1101 92-93 0.1669281407915264 1101 94-95 -0.08604225243310637 1101 96-97 -0.11714514572998169 1101 98-99 -0.3354336460120102 1101 100-101 -0.2672919771430813 1102 1 0.37589416016658816 1102 2 0.4629597745093754 1102 3 0.10979242950068624 1102 4 0.08507199007429733 1102 5 0.15134392838201904 1102 6 0.11642550783730599 1102 7 0.1106671411966289 1102 8 -0.15875632773137482 1102 9 -0.06591410617256344 1102 10-11 0.15172159640028582 1102 12-13 0.06391279396674321 1102 14-15 0.14138105490369668 1102 16-17 0.25829771440615445 1102 18-19 0.1425808980441161 1102 20-21 0.11032945432462782 1102 22-23 0.08968658312925726 1102 24-25 0.16475101829495742 1102 26-27 -0.18973690114895447 1102 28-29 -0.0031552918505610705 1102 30-31 -0.025593094661061855 1102 32-33 0.2519133459605314 1102 34-35 0.2572947990438692 1102 36-37 0.23690233202981403 1102 38-39 0.23002004647631935 1102 40-41 0.3759674660585972 1102 42-43 0.29816935560272384 1102 44-45 0.30793112595431893 1102 46-47 0.2597805848258474 1102 48-49 0.23602913667996006 1102 50-51 0.13014254156605887 1102 52-53 0.10898120456279514 1102 54-55 0.39220685550943557 1102 56-57 0.2179539612149881 1102 58-59 0.2062874740274765 1102 60-61 0.2542125753457114 1102 62-63 0.14843352230251838 1102 64-65 -0.018698478551044673 1102 66-67 0.30443899041519273 1102 68-69 0.1833245258812184 1102 70-71 0.11201698058299314 1102 72-73 0.06958043886701404 1102 74-75 -0.4378410623448552 1102 76-77 -0.0985112471322509 1102 78-79 0.11811948674522554 1102 80-81 0.06283375697805127 1102 82-83 -0.0014091988912809938 1102 84-85 0.19103987432108838 1102 86-87 0.12434506459491956 1102 88-89 0.15140731915001027 1102 90-91 0.1155547832998316 1102 92-93 0.21889089518692018 1102 94-95 -0.01747340587933266 1102 96-97 0.012361071459800144 1102 98-99 -0.35025681288471944 1102 100-101 -0.40676337919269656 1103 1 0.3108729727253525 1103 2 0.3820204574591024 1103 3 0.10083059265471661 1103 4 0.09745415684615466 1103 5 -0.01769235430609939 1103 6 -0.015923228429493008 1103 7 -0.013586718838894285 1103 8 -0.013185941205470897 1103 9 -0.09435679704200339 1103 10-11 -0.03494426885016111 1103 12-13 0.049113355530529645 1103 14-15 -0.025871074931451687 1103 16-17 0.06620025074537494 1103 18-19 0.10719994627648788 1103 20-21 -0.035813801686352065 1103 22-23 -0.08681460539961705 1103 24-25 -0.1545310558847035 1103 26-27 -0.13730116828960348 1103 28-29 -0.10423992283416794 1103 30-31 -0.03651332083217085 1103 32-33 -0.11504447807995177 1103 34-35 -0.07251559446580558 1103 36-37 -0.010923397151614722 1103 38-39 -0.10148455522697475 1103 40-41 -0.053222044548000724 1103 42-43 -0.047704209540249565 1103 44-45 0.02701177275444877 1103 46-47 -0.03189105491466648 1103 48-49 0.09271466524973704 1103 50-51 -0.09983199915804875 1103 52-53 0.03769315697011422 1103 54-55 0.19306172332584737 1103 56-57 0.06657774352744639 1103 58-59 0.11674309345781353 1103 60-61 0.1539317218946863 1103 62-63 -0.014760364609067267 1103 64-65 -0.05194415698564825 1103 66-67 0.11447594142720163 1103 68-69 0.13166037079573556 1103 70-71 0.11510322012344076 1103 72-73 0.09246831358275998 1103 74-75 0.24837407783858367 1103 76-77 0.15382810111600875 1103 78-79 0.1693935238651072 1103 80-81 0.18698851331174637 1103 82-83 0.1421970023529191 1103 84-85 -0.027003868693352473 1103 86-87 0.07354357682295642 1103 88-89 -0.05647310668821959 1103 90-91 -0.1059572315034707 1103 92-93 0.0019564696377720736 1103 94-95 0.15846027979091915 1103 96-97 -0.21613779009983958 1103 98-99 -0.31531306228544764 1103 100-101 -0.330134035624134 1104 1 0.34825139686887496 1104 2 0.3961488954834387 1104 3 0.20254590450975485 1104 4 0.14341285171150275 1104 5 0.03473479816220504 1104 6 0.129490358849921 1104 7 0.1571318083165707 1104 8 0.0488786504359382 1104 9 0.12687318230157274 1104 10-11 0.1585830276519573 1104 12-13 0.12067910202566878 1104 14-15 0.31311226779926216 1104 16-17 0.11420276502776261 1104 18-19 0.16526359847653538 1104 20-21 0.014106045912740228 1104 22-23 0.015547091996346296 1104 24-25 0.02227290758396805 1104 26-27 0.13182703368672577 1104 28-29 -0.04453610814996978 1104 30-31 -0.07235142040523357 1104 32-33 0.0630679187752392 1104 34-35 0.13215123787983174 1104 36-37 0.058421790839098264 1104 38-39 0.0089025516231942 1104 40-41 0.17071023906946436 1104 42-43 0.3074479018720382 1104 44-45 0.37356817382703156 1104 46-47 0.22645613696029443 1104 48-49 0.2108137127737706 1104 50-51 0.2606217652731715 1104 52-53 0.21095231778532053 1104 54-55 0.2572867480366554 1104 56-57 0.21890709195478308 1104 58-59 0.26623392752082253 1104 60-61 0.11974803676075396 1104 62-63 -0.08134423632484555 1104 64-65 0.03624701458146262 1104 66-67 0.1951757807067125 1104 68-69 0.30117431084072877 1104 70-71 0.21976267411323036 1104 72-73 0.19237867472433123 1104 74-75 0.06441377725297315 1104 76-77 0.4040989624893214 1104 78-79 0.45930545569087755 1104 80-81 0.4912127482267188 1104 82-83 0.5501890533250346 1104 84-85 0.47926417079287376 1104 86-87 0.4558617701263792 1104 88-89 0.32857337101533446 1104 90-91 0.21699474029903598 1104 92-93 0.16677580087072386 1104 94-95 0.257315625120242 1104 96-97 -0.09123462174179409 1104 98-99 0.0926017905972607 1104 100-101 -0.08676713299853844 1105 1 0.27682635273458445 1105 2 0.33123084707108674 1105 3 0.04063729654782122 1105 4 -0.006426295830827655 1105 5 -0.12614101803409739 1105 6 -0.052512539175062045 1105 7 -0.020311317198512313 1105 8 -0.06822657909921048 1105 9 -0.0851984101714649 1105 10-11 -0.1232477070583542 1105 12-13 -0.08823203477111718 1105 14-15 -0.042425905598449276 1105 16-17 0.018249778687170704 1105 18-19 -0.03070377922473 1105 20-21 -0.00367504456047385 1105 22-23 -0.02591413590652536 1105 24-25 -0.14046100885552448 1105 26-27 -0.2019919427489114 1105 28-29 -0.12997571953933118 1105 30-31 -0.14858455856684571 1105 32-33 -0.17296411344636198 1105 34-35 -0.16599135165895973 1105 36-37 -0.14373183395743183 1105 38-39 -0.19706854619776948 1105 40-41 -0.1413404718214295 1105 42-43 -0.18589201783420606 1105 44-45 -0.3775378680831878 1105 46-47 -0.20886081081081898 1105 48-49 -0.10078903174336062 1105 50-51 -0.2427069815324785 1105 52-53 -0.24537672202090022 1105 54-55 -0.18468675273393131 1105 56-57 -0.15734539145634585 1105 58-59 -0.07787212834180224 1105 60-61 -0.17925991564178645 1105 62-63 -0.38160586749935277 1105 64-65 -0.14603853729195038 1105 66-67 -0.11979114032751426 1105 68-69 -0.30043332015475244 1105 70-71 -0.22689444638870881 1105 72-73 -0.33920220997437767 1105 74-75 -0.2744077647013654 1105 76-77 -0.286600989084846 1105 78-79 -0.4687158717412636 1105 80-81 -0.5033872777655191 1105 82-83 -0.253472497615288 1105 84-85 -0.18732225646091294 1105 86-87 -0.3040627426406246 1105 88-89 -0.28253765300208755 1105 90-91 -0.634952569135752 1105 92-93 -0.6330977495484973 1105 94-95 -0.4489960525596821 1105 96-97 -0.5806253827704282 1105 98-99 -0.48740994196736054 1105 100-101 -0.5020454435719373 1106 1 0.32999838670057713 1106 2 0.33652711485658315 1106 3 0.10377838591331567 1106 4 0.06182090751478597 1106 5 -0.03524104165462916 1106 6 0.026430178477973243 1106 7 0.0046214322487898585 1106 8 0.03059936134645369 1106 9 0.0026548570243676295 1106 10-11 -0.06100708687485934 1106 12-13 -0.07258232560958078 1106 14-15 0.006699964665337177 1106 16-17 0.050295877815244694 1106 18-19 -0.002510971082948288 1106 20-21 -0.032294891587774543 1106 22-23 -0.08186090303694016 1106 24-25 -0.04325596391703357 1106 26-27 0.01445321767531027 1106 28-29 0.10360605424391167 1106 30-31 -0.07264623705227535 1106 32-33 -0.10836245932916455 1106 34-35 -0.30939680409417036 1106 36-37 -0.13813665594526015 1106 38-39 -0.033123848838002345 1106 40-41 -0.1041011686102209 1106 42-43 -0.10525762803419525 1106 44-45 -0.03204837072477318 1106 46-47 -0.0957006890716059 1106 48-49 0.08423529400931073 1106 50-51 0.16813187033957888 1106 52-53 0.32050027106796364 1106 54-55 0.16261438869869238 1106 56-57 0.049844937378885845 1106 58-59 -0.04322369197026177 1106 60-61 0.22395315688006434 1106 62-63 -0.23555567980956482 1106 64-65 -0.049453064091061094 1106 66-67 0.16892708028149173 1106 68-69 0.1464664421105084 1106 70-71 0.08685924750615115 1106 72-73 0.09178181472692515 1106 74-75 0.05246458754708527 1106 76-77 0.1528278017986473 1106 78-79 0.3074478644496459 1106 80-81 0.21143018987519469 1106 82-83 0.3104292919376377 1106 84-85 0.4639948857601972 1106 86-87 0.14947073649428688 1106 88-89 0.22410940352236253 1106 90-91 0.22046766397683726 1106 92-93 0.5476897489008721 1106 94-95 0.5994822072016284 1106 96-97 0.5038232986429314 1106 98-99 0.4690606402703352 1106 100-101 0.6638776860300304 1107 1 0.5141256263750975 1107 2 0.5195106195824586 1107 3 0.339548109471572 1107 4 0.155006796307525 1107 5 0.01955090591467723 1107 6 0.11807319901154045 1107 7 0.1535377349997873 1107 8 0.20709275719949005 1107 9 0.37159960221632105 1107 10-11 0.28951648211482706 1107 12-13 0.36947827557239776 1107 14-15 0.4456563819910784 1107 16-17 0.38826049917525296 1107 18-19 0.34845499860974627 1107 20-21 0.300893210885512 1107 22-23 0.35751544278021186 1107 24-25 0.3314594439703811 1107 26-27 0.3184277804734066 1107 28-29 0.4729029758863277 1107 30-31 0.4122269458849459 1107 32-33 0.3875952258317952 1107 34-35 0.38001945505403256 1107 36-37 0.46846011208113936 1107 38-39 0.4891363860804603 1107 40-41 0.5354361721557055 1107 42-43 0.45969041755419937 1107 44-45 0.26486555735072415 1107 46-47 0.5851814961664985 1107 48-49 0.5200965834446833 1107 50-51 0.5288158976282205 1107 52-53 0.3503742254393174 1107 54-55 0.5818355085527216 1107 56-57 0.6418300721786139 1107 58-59 0.4445453876082084 1107 60-61 0.6007511015112144 1107 62-63 0.4028570441578907 1107 64-65 0.5352151856926071 1107 66-67 0.46574877166759165 1107 68-69 0.42854535646790737 1107 70-71 0.3306200224285014 1107 72-73 0.5172190166347157 1107 74-75 0.6920592827635232 1107 76-77 0.6609392639681886 1107 78-79 0.651866035412862 1107 80-81 0.688420993038747 1107 82-83 0.7545167524435641 1107 84-85 0.7034698409834768 1107 86-87 1.0130047097825425 1107 88-89 0.9764636192421783 1107 90-91 0.8389264056671486 1107 92-93 1.0697923429319829 1107 94-95 1.1273601596142093 1107 96-97 1.0955682826440558 1107 98-99 1.1036852872553702 1107 100-101 1.0419853916507655 1108 1 0.3576156388263101 1108 2 0.44623047371157654 1108 3 0.18700463089555086 1108 4 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6 152.0 7 339.0 8 868.0 9 1612.0 10 2771.0 11 4354.0 12 6129.0 13 8305.0 14 10761.0 15 14337.0 16 18318.0 17 22816.0 18 20428.0 19 13971.0 20 13020.0 21 13432.0 22 14344.0 23 16256.0 24 18607.0 25 22332.0 26 27549.0 27 34071.0 28 42886.0 29 53249.0 30 65602.0 31 81329.0 32 101809.0 33 130871.0 34 172060.0 35 233713.0 36 330169.0 37 424061.0 38 257272.0 39 15115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87802168382569 19.20491717541652 15.03878233646931 21.878278804288477 2 15.709179897087521 21.05656081873932 39.07659696534514 24.15766231882802 3 20.929471116597497 24.322735661358482 29.345575248882415 25.402217973161605 4 13.29562701574654 16.78697298717219 36.93736956934168 32.98003042773959 5 13.650710992105763 37.96124199267485 33.62739125359327 14.760655761626126 6 31.97521924384585 36.344455428929976 16.7096368796603 14.970688447563877 7 28.82741934307102 32.96997891465176 19.92387569129299 18.278726050984226 8 26.683271074404285 33.77732340108856 18.973439027593354 20.565966496913806 9 26.110199337229815 17.43905492091128 20.2149921770865 36.2357535647724 10-11 25.527733767505527 26.379700184976144 26.425801720987213 21.666764326531116 12-13 27.284137604916758 23.69248630350775 26.836301239153787 22.187074852421706 14-15 23.378446778143083 25.350731193484695 26.304109913861893 24.966712114510326 16-17 22.15071920007004 28.400126402649896 26.849641950358233 22.599512446921832 18-19 21.900695488173234 27.39870916081773 29.172815039908944 21.527780311100088 20-21 23.29340462710306 25.699523755672615 28.973999905152414 22.03307171207191 22-23 22.517260382164615 25.778065263978224 29.137595359322155 22.567078994535 24-25 22.944155747034188 25.878115378441873 29.356605040054866 21.821123834469073 26-27 22.595213569047935 25.600948658704027 29.911499864796525 21.892337907451513 28-29 21.906235864061518 26.934069982927433 29.385811712947422 21.773882440063623 30-31 22.395359759962428 25.961221893396687 29.09178792424954 22.551630422391348 32-33 22.360060376394834 26.016617189031926 29.250128824256354 22.373193610316886 34-35 23.156412735182265 25.822738921467593 29.116065809993707 21.90478253335644 36-37 23.034970092538252 25.59184488704843 29.50500950554304 21.86817551487028 38-39 22.492041683747615 26.104782103330205 29.52944588413348 21.8737303287887 40-41 22.66193324646276 26.243372128682452 29.10611092300554 21.988583701849247 42-43 22.32516586779692 26.47048696737551 28.4335836770172 22.770763487810367 44-45 22.779308314809946 25.932004331155063 28.444033227410493 22.844654126624498 46-47 23.49028912156348 26.632102922406453 27.91952221502239 21.958085741007675 48-49 23.385179396895893 26.498975855359717 27.839108393334477 22.27673635440991 50-51 22.633858949922022 26.961303796660253 28.21409679978842 22.190740453629306 52-53 23.273158655308446 27.29751125083164 27.251431747842748 22.177898346017166 54-55 22.720983546599573 26.73259154167801 27.57439236336755 22.972032548354864 56-57 23.18553885020361 26.795513723773436 27.32298635146905 22.695961074553903 58-59 23.896779018360988 26.42771316459679 27.120815881694416 22.554691935347808 60-61 23.103905635971497 26.734810256606696 28.16266540256955 21.998618704852248 62-63 22.799737336519343 26.753461138573225 28.038733743137005 22.408067781770423 64-65 22.651794359353485 27.444118249346328 27.50308876910328 22.400998622196905 66-67 22.899929135775672 26.87564468659681 27.12734036625039 23.097085811377127 68-69 22.738987746860566 26.88996616034098 27.41523099295072 22.955815099847744 70-71 23.376590508896726 26.819296848405784 27.057672256727695 22.7464403859698 72-73 23.135566188925036 26.44627213557371 27.747189170591863 22.67097250490939 74-75 22.650584224657454 27.78703707081467 26.98735335103822 22.57502535348966 76-77 23.04094615259565 26.844243559691204 26.951518389140183 23.16329189857296 78-79 23.005904849291355 27.078942015912265 27.135354578264792 22.77979855653159 80-81 23.433217590824274 26.968179437298488 26.935479347065396 22.663123624811842 82-83 23.23804294463559 26.97362015383854 27.257646607336394 22.530690294189473 84-85 22.924639191359976 27.322839223966426 27.014446026630125 22.738075558043477 86-87 23.016029192160364 27.343138084272457 26.74556345242946 22.895269271137717 88-89 22.893432805049486 27.3705722816553 26.35165769469007 23.384337218605154 90-91 22.962665312339436 26.759395816196147 26.791916089485028 23.48602278197939 92-93 23.2752977571398 27.231659466655785 26.87029469389561 22.622748082308814 94-95 22.950514279642533 27.01347052625507 27.01073432917226 23.02528086493014 96-97 22.369524257749376 26.963683207590748 27.500241132976118 23.16655140168376 98-99 22.57817666078375 27.38828159559283 26.481217991690954 23.55232375193247 100-101 22.343668089311738 27.14080264180105 26.774458424628655 23.74107084425856 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 358.0 1 282.0 2 253.0 3 304.5 4 288.0 5 283.5 6 471.5 7 960.5 8 1564.5 9 1978.5 10 1919.0 11 1713.5 12 1764.0 13 1987.5 14 2219.5 15 2424.0 16 2681.0 17 2957.0 18 3234.0 19 3515.0 20 3936.0 21 4486.0 22 5147.5 23 6095.5 24 7322.5 25 8837.5 26 11069.5 27 13953.5 28 16762.5 29 20164.0 30 24178.0 31 28266.0 32 33094.0 33 36686.0 34 39449.5 35 42642.0 36 47753.0 37 52426.0 38 54329.5 39 55986.5 40 57903.5 41 60148.0 42 62053.0 43 64784.0 44 68164.0 45 72540.0 46 76709.0 47 89806.0 48 116901.5 49 114747.5 50 89031.0 51 78078.5 52 74965.5 53 74007.5 54 73387.5 55 69865.5 56 62710.5 57 56248.5 58 52486.5 59 47238.5 60 39254.5 61 32691.5 62 27217.5 63 21264.0 64 15161.0 65 11105.0 66 8862.0 67 7114.0 68 5660.0 69 4602.0 70 3586.0 71 2603.5 72 2073.5 73 1734.0 74 1346.5 75 1010.5 76 740.0 77 503.5 78 342.5 79 243.5 80 193.0 81 139.0 82 94.5 83 53.0 84 26.5 85 11.5 86 6.0 87 5.5 88 4.5 89 2.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0398122747369805 2 0.011627949395164233 3 0.004058382337920065 4 0.011673549196713895 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003419985116224774 10-11 2.9639871007281377E-4 12-13 1.5959930542382278E-4 14-15 9.11996030993273E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0016643927565627235 24-25 0.0 26-27 4.559980154966365E-5 28-29 0.0 30-31 9.11996030993273E-5 32-33 0.0037391837270724197 34-35 5.471976185959639E-4 36-37 0.002781587894529483 38-39 1.3679940464899097E-4 40-41 0.0033971852154499422 42-43 0.009416359020005545 44-45 0.001983591367410369 46-47 0.0017783922604368827 48-49 0.008504362989012271 50-51 5.471976185959639E-4 52-53 0.0019607914666355374 54-55 0.008800761699085086 56-57 0.0037391837270724197 58-59 0.0012311946418409186 60-61 0.005585975689833798 62-63 0.0032831857115757836 64-65 0.016917526374925215 66-67 0.0030095869022778014 68-69 1.3679940464899097E-4 70-71 0.0010259955348674323 72-73 2.2799900774831827E-5 74-75 0.0 76-77 0.0012083947410660868 78-79 0.009940756737826677 80-81 0.015389933023011483 82-83 0.01087555266959478 84-85 0.007957165370416307 86-87 0.009644358027753864 88-89 0.02781587894529483 90-91 0.023415498095752288 92-93 0.002530788986006333 94-95 0.007637966759568663 96-97 0.009963556638601509 98-99 0.00921115991303206 100-101 0.011194751280442427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2192992.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.07395709808816 #Duplication Level Percentage of deduplicated Percentage of total 1 77.52239480450939 43.46987440409112 2 13.271684830853522 14.883917716492556 3 4.145117869181671 6.972994846890349 4 1.7476285430379244 3.9198579178281157 5 0.9150891146871241 2.5656333878946636 6 0.5216094535087067 1.7549223670802656 7 0.3366742590483517 1.3215060570535526 8 0.24312669106566343 1.0906460515372915 9 0.1777811746963978 0.8972004566496173 >10 0.9948765881280273 10.339317840854518 >50 0.07701241051834827 2.9607455854328015 >100 0.041956593097913344 4.259813879230117 >500 0.002523833833524142 0.9235409395479227 >1k 0.0021167638603750132 2.6522927091376047 >5k 3.256559785192328E-4 1.2784399639693187 >10k+ 8.14139946298082E-5 0.7092958763102166 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15537 0.7084841166771242 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8643 0.394119084793743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7377 0.3363897360318688 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6606 0.3012322890370781 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5378 0.24523573273409113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4550 0.20747909705096965 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4539 0.20697749923392333 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4509 0.20560950518743343 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4122 0.1879623819877136 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3960 0.18057521413666808 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3269 0.14906575126585048 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3222 0.1469225605930163 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2668 0.12166027053450264 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2442 0.11135471538427864 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2273 0.10364834892238549 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2265 0.10328355050998818 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2240 0.10214355547124658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.559980154966365E-5 2 0.0 0.0 0.0 0.0 4.559980154966365E-5 3 0.0 0.0 0.0 4.559980154966365E-5 4.559980154966365E-5 4 0.0 0.0 0.0 1.3679940464899097E-4 4.559980154966365E-5 5 0.0 0.0 0.0 1.3679940464899097E-4 4.559980154966365E-5 6 0.0 0.0 0.0 1.3679940464899097E-4 4.559980154966365E-5 7 0.0 0.0 0.0 1.3679940464899097E-4 9.11996030993273E-5 8 0.0 0.0 0.0 1.3679940464899097E-4 9.11996030993273E-5 9 0.0 0.0 0.0 1.3679940464899097E-4 9.11996030993273E-5 10-11 0.0 0.0 0.0 1.3679940464899097E-4 9.11996030993273E-5 12-13 0.0 0.0 0.0 1.595993054238228E-4 1.823992061986546E-4 14-15 0.0 0.0 0.0 2.0519910697348645E-4 1.823992061986546E-4 16-17 0.0 0.0 0.0 2.279990077483183E-4 1.823992061986546E-4 18-19 0.0 0.0 0.0 2.279990077483183E-4 1.823992061986546E-4 20-21 0.0 0.0 0.0 2.507989085231501E-4 1.823992061986546E-4 22-23 0.0 0.0 0.0 4.103982139469729E-4 1.823992061986546E-4 24-25 0.0 0.0 0.0 6.839970232449549E-4 1.823992061986546E-4 26-27 0.0 0.0 0.0 8.435963286687776E-4 1.823992061986546E-4 28-29 0.0 0.0 0.0 0.0014135938480395733 1.823992061986546E-4 30-31 0.0 0.0 0.0 0.002257190176708351 1.823992061986546E-4 32-33 0.0 0.0 0.0 0.00348838481854927 1.823992061986546E-4 34-35 0.0 0.0 0.0 0.005129977674337162 2.279990077483183E-4 36-37 0.0 0.0 0.0 0.00786596576731698 2.279990077483183E-4 38-39 0.0 0.0 0.0 0.012289146517634356 2.507989085231501E-4 40-41 0.0 0.0 0.0 0.016507128160978244 2.7359880929798193E-4 42-43 0.0 0.0 0.0 0.02058831039967314 2.7359880929798193E-4 44-45 0.0 0.0 0.0 0.024031095416672747 2.9639871007281377E-4 46-47 0.0 0.0 0.0 0.028978673884811255 3.419985116224774E-4 48-49 0.0 0.0 0.0 0.03244425880258569 4.103982139469729E-4 50-51 0.0 0.0 0.0 0.03611504282733362 4.559980154966366E-4 52-53 0.0 0.0 0.0 0.04464220571712072 4.559980154966366E-4 54-55 0.0 0.0 0.0 0.05987253943470838 4.559980154966366E-4 56-57 0.0 0.0 0.0 0.0826040405072157 4.559980154966366E-4 58-59 0.0 0.0 0.0 0.11333830675168902 4.559980154966366E-4 60-61 0.0 0.0 2.2799900774831827E-5 0.14888335205965184 4.559980154966366E-4 62-63 0.0 0.0 4.559980154966365E-5 0.17077125680349037 4.559980154966366E-4 64-65 0.0 0.0 4.559980154966365E-5 0.19810833783251375 4.559980154966366E-4 66-67 0.0 0.0 4.559980154966365E-5 0.2522353022719645 4.559980154966366E-4 68-69 0.0 0.0 4.559980154966365E-5 0.32831857115757834 4.787979162714684E-4 70-71 0.0 2.2799900774831827E-5 4.559980154966365E-5 0.40342144430987437 5.015978170463002E-4 72-73 0.0 4.559980154966365E-5 4.559980154966365E-5 0.48784947687907665 5.015978170463002E-4 74-75 0.0 4.559980154966365E-5 4.559980154966365E-5 0.5650043411011075 5.015978170463002E-4 76-77 0.0 4.559980154966365E-5 4.559980154966365E-5 0.6333584436240534 5.015978170463002E-4 78-79 0.0 4.559980154966365E-5 4.559980154966365E-5 0.7056341290802702 5.471976185959639E-4 80-81 0.0 4.559980154966365E-5 4.559980154966365E-5 0.7774994163225402 5.471976185959639E-4 82-83 0.0 4.559980154966365E-5 4.559980154966365E-5 0.8748093928295224 5.471976185959639E-4 84-85 0.0 4.559980154966365E-5 4.559980154966365E-5 1.0506422276050253 5.471976185959639E-4 86-87 0.0 4.559980154966365E-5 4.559980154966365E-5 1.2908847820694285 6.155973209204593E-4 88-89 0.0 4.559980154966365E-5 4.559980154966365E-5 1.5691575710262509 6.839970232449549E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 10355 0.0 23.1663 2 GTACATG 10760 0.0 23.01776 1 GAGTACT 9610 0.0 21.768026 12-13 ACATGGG 10680 0.0 21.705208 3 AGTACTT 9960 0.0 21.36069 12-13 GTACTTT 10095 0.0 20.722214 14-15 TAAGGTG 965 0.0 20.178219 5 GTACCTG 1740 0.0 19.309649 1 TACTTTT 11240 0.0 18.928154 14-15 TATACTG 965 0.0 18.701763 5 GTAAGGT 870 0.0 18.562033 4 GTATAGC 835 0.0 18.394547 1 TACCTGG 1705 0.0 18.38802 2 ACTTTTT 11655 0.0 18.254805 16-17 CATGGGA 5885 0.0 18.159418 4 GTGTAGC 1235 0.0 17.8779 1 CATGGGG 4515 0.0 17.778488 4 AGAGTAC 18445 0.0 17.63634 10-11 GTATAGG 570 0.0 16.8415 1 CTAGACT 1370 0.0 16.29458 4 >>END_MODULE