FastQCFastQC Report
Thu 26 May 2016
SRR936661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274743
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128930.5667893032311782No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT90900.3996055818173745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76720.33726886949426815No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61800.27167904242369356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60010.26381002161562866No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56550.2485995121207099No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40470.17791020787842848No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38940.17118417333298752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.15043457656535264No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33180.14586263151485684No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31300.13759796161588364No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29090.12788257838358005No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26570.11680440383814789No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24050.10572622929271572No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23300.10242915353514662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG110600.023.7595122
GTACATG114050.023.6241021
GAGTACT79150.022.84395412-13
ACATGGG110700.022.8366053
GTACTTT82550.021.67234614-15
GTGTACG2205.6215868E-821.5995561
AGTACTT82450.019.97009312-13
GTATAGG6500.019.7386721
GTGTAGC13800.019.2830831
TACCTGG17200.018.786592
CATGGGG48300.018.6944274
CGTACAC2051.5850319E-518.544013
CATGGGA59800.018.3576264
ACTTTTT101000.018.20638316-17
GTACCTG18350.018.1271481
TATAGCG1350.00938149217.5996385
AGAGTAC163000.016.82078410-11
TAAGGTG8200.016.805515
TACTTTT96450.016.75076714-15
GTCCTAC5250.016.2922381
Produced by FastQC (version 0.11.5)