##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936661_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2274743 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3712155614942 31.0 31.0 34.0 30.0 34.0 2 31.40849449805978 31.0 31.0 34.0 28.0 34.0 3 31.46613705372431 31.0 31.0 34.0 28.0 34.0 4 35.07668646524025 37.0 35.0 37.0 32.0 37.0 5 35.053056543090804 37.0 35.0 37.0 32.0 37.0 6 35.178439058829944 37.0 35.0 37.0 32.0 37.0 7 35.043518322729206 37.0 35.0 37.0 32.0 37.0 8 34.989277909636385 37.0 35.0 37.0 32.0 37.0 9 36.45975743193846 39.0 37.0 39.0 32.0 39.0 10-11 36.36985584745178 39.0 37.0 39.0 32.0 39.0 12-13 36.26745944486916 39.0 35.0 39.0 32.0 39.0 14-15 37.309490786431695 40.0 37.0 41.0 32.0 41.0 16-17 37.190339304264256 39.0 36.5 41.0 31.0 41.0 18-19 37.15616071793605 39.0 36.5 41.0 31.0 41.0 20-21 37.051407785406965 39.0 36.0 41.0 31.0 41.0 22-23 36.915156789140575 39.0 36.0 41.0 31.0 41.0 24-25 37.05346538048474 39.0 36.0 41.0 31.0 41.0 26-27 37.02886677747772 39.0 36.0 41.0 31.0 41.0 28-29 36.94361428961426 39.0 36.0 41.0 30.0 41.0 30-31 36.76062350779846 39.0 36.0 41.0 30.0 41.0 32-33 36.57753227507459 39.0 35.5 41.0 30.0 41.0 34-35 36.35092447806192 39.0 35.0 40.5 30.0 41.0 36-37 36.151443481747165 39.0 35.0 40.0 29.5 41.0 38-39 35.93865328962437 38.0 35.0 40.0 29.0 41.0 40-41 35.76977443166107 38.0 35.0 40.0 28.0 41.0 42-43 35.53466633373529 38.0 35.0 40.0 27.0 41.0 44-45 35.25398319722272 38.0 34.0 40.0 26.5 41.0 46-47 34.96523497379704 38.0 34.0 40.0 25.0 41.0 48-49 34.84849717088919 38.0 34.0 40.0 24.5 41.0 50-51 33.92315813258904 37.5 33.0 39.5 23.5 40.5 52-53 34.07303967964732 37.5 33.0 39.5 24.0 40.5 54-55 34.49650290164647 38.0 34.0 40.0 24.0 41.0 56-57 34.37430360264874 38.0 34.0 40.0 23.5 41.0 58-59 34.10242783470484 38.0 33.5 40.0 23.0 41.0 60-61 33.74838893888233 37.0 33.0 40.0 22.0 41.0 62-63 33.433274879843566 37.0 33.0 40.0 22.0 41.0 64-65 33.084282927785694 36.0 32.0 39.0 20.5 41.0 66-67 32.65312191311283 36.0 32.0 39.0 19.5 41.0 68-69 32.22969254109145 35.0 31.0 39.0 18.0 40.0 70-71 31.769093036004506 35.0 31.0 38.0 16.5 40.0 72-73 31.367757588439662 35.0 31.0 37.0 13.5 39.5 74-75 30.940734403842544 35.0 30.0 37.0 9.5 39.0 76-77 30.50965735469897 34.5 30.0 36.0 7.5 39.0 78-79 30.054174911187772 34.0 29.5 36.0 7.0 38.5 80-81 29.56090995774028 34.0 29.0 35.0 2.0 37.0 82-83 29.078165313620044 34.0 29.0 35.0 2.0 37.0 84-85 28.75176272660252 34.0 29.0 35.0 2.0 36.0 86-87 28.45010996846677 34.0 28.5 35.0 2.0 36.0 88-89 28.02315206596965 34.0 27.0 35.0 2.0 35.5 90-91 27.591659145670523 33.0 26.5 35.0 2.0 35.0 92-93 27.204990190100595 33.0 26.0 35.0 2.0 35.0 94-95 26.731055112599535 33.0 25.0 35.0 2.0 35.0 96-97 26.132400671196702 33.0 24.5 35.0 2.0 35.0 98-99 25.41430548417997 32.5 21.0 35.0 2.0 35.0 100-101 22.903177633693126 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3137145813984574 1101 2 0.3998368166262338 1101 3 0.3476662105519388 1101 4 0.396810297509937 1101 5 0.3334187179201038 1101 6 0.19520077928629576 1101 7 0.3642554353885217 1101 8 0.44222987433089145 1101 9 0.5648367371181919 1101 10-11 0.45787933609113196 1101 12-13 0.6093009433992975 1101 14-15 0.7135947709573571 1101 16-17 0.698174109178261 1101 18-19 0.598685222124594 1101 20-21 0.6355540148487719 1101 22-23 0.5860369549988604 1101 24-25 0.5836695924846182 1101 26-27 0.5177769133145915 1101 28-29 0.44083022689350315 1101 30-31 0.5528903140032284 1101 32-33 0.5616317916565023 1101 34-35 0.439428701654748 1101 36-37 0.5402086475433023 1101 38-39 0.3676956135203966 1101 40-41 0.38426836028053657 1101 42-43 0.43779539002713364 1101 44-45 0.40356050290090195 1101 46-47 0.5755310966405816 1101 48-49 0.7404599856569547 1101 50-51 0.6859220315637558 1101 52-53 0.7959462190213955 1101 54-55 0.8512433403008615 1101 56-57 0.7067317017261203 1101 58-59 0.8531508642237 1101 60-61 0.764010756424554 1101 62-63 0.7208627347109342 1101 64-65 0.69388085998321 1101 66-67 0.7339394543980262 1101 68-69 0.5921236343334328 1101 70-71 0.5462499085393411 1101 72-73 0.5813011371298309 1101 74-75 0.5306389477757882 1101 76-77 0.5735057913997359 1101 78-79 0.5990566523973193 1101 80-81 0.6726798291416323 1101 82-83 0.6916304522625119 1101 84-85 0.7469795019164742 1101 86-87 0.5645590339872477 1101 88-89 0.5925127061155138 1101 90-91 0.48563287877400896 1101 92-93 0.42600682075736174 1101 94-95 0.4989407354514377 1101 96-97 0.48027357518311575 1101 98-99 0.6564618215997378 1101 100-101 1.2180756231076977 1102 1 0.23724616617865735 1102 2 0.39170973868017 1102 3 0.2508801004669756 1102 4 0.31443128014754507 1102 5 0.38328942349823336 1102 6 0.1708195454480972 1102 7 0.21279625548456949 1102 8 0.3133048650955814 1102 9 0.3908433865456047 1102 10-11 0.35795321861791507 1102 12-13 0.31912731946135864 1102 14-15 0.34436684336223067 1102 16-17 0.20851766292779672 1102 18-19 0.28080565064325214 1102 20-21 0.4381029620227679 1102 22-23 0.37547175366897534 1102 24-25 0.17085097408786964 1102 26-27 0.18438201120893893 1102 28-29 0.27422512899915574 1102 30-31 0.23353309198623862 1102 32-33 0.13994727004586593 1102 34-35 -0.005159403258446105 1102 36-37 0.22214436974500273 1102 38-39 0.1550986427039902 1102 40-41 0.16871608839284136 1102 42-43 0.25253495412022886 1102 44-45 -0.036409944088383384 1102 46-47 0.06463342394017957 1102 48-49 0.13203737760376555 1102 50-51 0.2561104320070555 1102 52-53 0.17219298666083915 1102 54-55 0.33147976438656457 1102 56-57 0.41083218196254023 1102 58-59 0.5637160655535922 1102 60-61 0.34546624220216415 1102 62-63 0.38986901472570423 1102 64-65 0.39484133283138334 1102 66-67 0.47867532436477234 1102 68-69 0.28606526713727476 1102 70-71 0.18182434518508117 1102 72-73 0.18030372301826603 1102 74-75 0.176187525537145 1102 76-77 0.42943486417402355 1102 78-79 0.3509960687253546 1102 80-81 0.485941742699076 1102 82-83 0.5538395398133034 1102 84-85 0.46881178858067685 1102 86-87 0.39647628555725234 1102 88-89 0.31138969170842046 1102 90-91 0.3881079434212218 1102 92-93 0.2398228532656752 1102 94-95 0.34138624708793586 1102 96-97 0.28503899816058365 1102 98-99 0.3840200040895745 1102 100-101 0.548699930459005 1103 1 0.1864264641166038 1103 2 0.29290071348062696 1103 3 0.2504710147556928 1103 4 0.2820746595414576 1103 5 0.3209774330450301 1103 6 0.15014795025954442 1103 7 0.2156708376302845 1103 8 0.18976386310739457 1103 9 0.4417528678208029 1103 10-11 0.37730750290530324 1103 12-13 0.29993193339907975 1103 14-15 0.28631703182308144 1103 16-17 0.3554129975249012 1103 18-19 0.2855712932640557 1103 20-21 0.4715128410583205 1103 22-23 0.5068778402877996 1103 24-25 0.45013152410772506 1103 26-27 0.28024603111850865 1103 28-29 0.08936705222640029 1103 30-31 0.09248788897608051 1103 32-33 0.34067752922363326 1103 34-35 0.3152731787985914 1103 36-37 0.3464958556390272 1103 38-39 0.3244617058609265 1103 40-41 0.26151663288867155 1103 42-43 0.37816792159640045 1103 44-45 0.5559655219158373 1103 46-47 0.517706350881177 1103 48-49 0.44083174572901385 1103 50-51 0.4636091378273406 1103 52-53 0.45474972293660443 1103 54-55 0.48129786538597585 1103 56-57 0.4770307612270912 1103 58-59 0.4262846817882249 1103 60-61 0.44319572041059985 1103 62-63 0.428031769613213 1103 64-65 0.4339716166091989 1103 66-67 0.4599523773881913 1103 68-69 0.5206606161622105 1103 70-71 0.5197340160399371 1103 72-73 0.4446616362253799 1103 74-75 0.2955766507485329 1103 76-77 0.25813008529788917 1103 78-79 0.33694713191578174 1103 80-81 0.3687869393121197 1103 82-83 0.21799328081290525 1103 84-85 0.40276956791517904 1103 86-87 0.4122212807436334 1103 88-89 0.48411535704583386 1103 90-91 0.6393620039057772 1103 92-93 0.6472322054317736 1103 94-95 0.7185536634933989 1103 96-97 0.6582528345375529 1103 98-99 0.4341392498288741 1103 100-101 0.8052294120169954 1104 1 0.27112785538053075 1104 2 0.34905538369287825 1104 3 0.3398861908942621 1104 4 0.3495021356412735 1104 5 0.3595094932579386 1104 6 0.2992134267524662 1104 7 0.4239846903082807 1104 8 0.3425300592352869 1104 9 0.4505846675373206 1104 10-11 0.4950632431694899 1104 12-13 0.34883673985137875 1104 14-15 0.528100180355267 1104 16-17 0.3890125202257124 1104 18-19 0.512879976861349 1104 20-21 0.6069792189771945 1104 22-23 0.43682092048149457 1104 24-25 0.46815612943854745 1104 26-27 0.12131056940882701 1104 28-29 0.3356121425675411 1104 30-31 0.38909959991347165 1104 32-33 0.21922370232627486 1104 34-35 0.1931188310934715 1104 36-37 0.3055526773838082 1104 38-39 0.3640362213067476 1104 40-41 0.6043183830805603 1104 42-43 0.5845801065025853 1104 44-45 0.4206924205627729 1104 46-47 0.4797293559161844 1104 48-49 0.3194344927896182 1104 50-51 0.1991491544024413 1104 52-53 0.31223935353498433 1104 54-55 0.4103625904059456 1104 56-57 0.2809364564954109 1104 58-59 0.4068921964323451 1104 60-61 0.32035714804410986 1104 62-63 0.41234156946096334 1104 64-65 0.4119985759859688 1104 66-67 0.36576812333681374 1104 68-69 0.35415278355110047 1104 70-71 0.5389248988512811 1104 72-73 0.4159693172894414 1104 74-75 0.36697910564556935 1104 76-77 0.5562495100054967 1104 78-79 0.42191367357890996 1104 80-81 0.533018933244545 1104 82-83 0.5396404981356078 1104 84-85 0.3941975814320138 1104 86-87 0.6146757081077219 1104 88-89 0.7052344226689193 1104 90-91 0.5936797781909142 1104 92-93 0.531677085388953 1104 94-95 0.5561488304975875 1104 96-97 0.6463605616008685 1104 98-99 0.9505559449960082 1104 100-101 1.4356876781554142 1105 1 0.4427043972660556 1105 2 0.4211823713732308 1105 3 0.489795608189862 1105 4 0.44692124735420435 1105 5 0.5118718622059149 1105 6 0.42675369004577846 1105 7 0.3867943516229104 1105 8 0.46522807235059815 1105 9 0.5491995047736609 1105 10-11 0.486862137419358 1105 12-13 0.39456305508410594 1105 14-15 0.2426556516720737 1105 16-17 0.39267549629229137 1105 18-19 0.33976937924612827 1105 20-21 0.4449198189248307 1105 22-23 0.32711206588568587 1105 24-25 0.4607319300880235 1105 26-27 0.5158135837841371 1105 28-29 0.36620961633791893 1105 30-31 0.2646608890338129 1105 32-33 0.3036282096441525 1105 34-35 0.36209674176723183 1105 36-37 0.3345502129007869 1105 38-39 0.29919982291136193 1105 40-41 0.10400496061951969 1105 42-43 0.5038626373632056 1105 44-45 0.18442992613818632 1105 46-47 0.374633354239684 1105 48-49 0.4935533159570795 1105 50-51 0.509669939992726 1105 52-53 0.46940791793095826 1105 54-55 0.4300011943975477 1105 56-57 0.6221509643037564 1105 58-59 0.5819111578377871 1105 60-61 0.3638786665678566 1105 62-63 0.3237893844290056 1105 64-65 0.4004794356906274 1105 66-67 0.610479186573123 1105 68-69 0.6665338087077686 1105 70-71 0.6527201611828879 1105 72-73 0.6978574965809266 1105 74-75 0.7074913534142873 1105 76-77 0.39019533811960727 1105 78-79 0.48894195355611103 1105 80-81 0.4902338844722607 1105 82-83 0.502839612034883 1105 84-85 0.6343216988791092 1105 86-87 0.7206400983345986 1105 88-89 0.4802432979750577 1105 90-91 0.5034965970997689 1105 92-93 0.6773261251108131 1105 94-95 0.8104454549907274 1105 96-97 1.1079209149895028 1105 98-99 1.1427542549286152 1105 100-101 1.6187847440999015 1106 1 0.11236982126901296 1106 2 0.024525848321960808 1106 3 0.23219877015825574 1106 4 0.2440349413349736 1106 5 0.26028437056518783 1106 6 0.18911229476290004 1106 7 0.3222083342809441 1106 8 0.2824893495196008 1106 9 0.5158233939461212 1106 10-11 0.381991952199634 1106 12-13 0.4726109848750326 1106 14-15 0.7093733613713695 1106 16-17 0.6640752835546735 1106 18-19 0.7564349210663366 1106 20-21 0.50490929702773 1106 22-23 0.41308866341345407 1106 24-25 0.3166350159834579 1106 26-27 0.13137980899438872 1106 28-29 0.31397141351926905 1106 30-31 0.23071327288341337 1106 32-33 0.26010106776568875 1106 34-35 0.2739671881179717 1106 36-37 0.2395191846361442 1106 38-39 0.22458517447753934 1106 40-41 0.13832035990085245 1106 42-43 0.03894368213392596 1106 44-45 0.31701857778723763 1106 46-47 0.3310389715395914 1106 48-49 0.48477093141923433 1106 50-51 0.28674936865071743 1106 52-53 0.46799344433994605 1106 54-55 0.6925432740703386 1106 56-57 0.5603193209897412 1106 58-59 0.5713774290251052 1106 60-61 0.6436975546229746 1106 62-63 0.7335645334544978 1106 64-65 0.5633572861973661 1106 66-67 0.6256691964701062 1106 68-69 0.6053687410728372 1106 70-71 0.6236790419623226 1106 72-73 0.8115658920516147 1106 74-75 0.6046144794409223 1106 76-77 0.6769341982651973 1106 78-79 0.7218424959654044 1106 80-81 0.8279522859104631 1106 82-83 1.1270504072613328 1106 84-85 1.1359338954809353 1106 86-87 1.1274900633143794 1106 88-89 1.035259038326501 1106 90-91 1.0674394522333763 1106 92-93 1.2760903104661772 1106 94-95 1.383866016417425 1106 96-97 1.4771024383782212 1106 98-99 1.8428858536320583 1106 100-101 2.1244733009452226 1107 1 0.13112784260338373 1107 2 0.17602005391947273 1107 3 0.032151927469712405 1107 4 0.13782200132789058 1107 5 0.21816213018983888 1107 6 0.174055988327936 1107 7 0.25205474357940716 1107 8 0.19190968076976844 1107 9 0.12020642242114832 1107 10-11 0.25454556422792507 1107 12-13 0.307585716481654 1107 14-15 0.20989224887655666 1107 16-17 0.17846036853796932 1107 18-19 0.33319885295782825 1107 20-21 0.50066679661834 1107 22-23 0.5643572243527473 1107 24-25 0.4416126419350732 1107 26-27 0.2698113786814602 1107 28-29 0.32211651921998197 1107 30-31 0.3531440656673581 1107 32-33 0.23111763027402077 1107 34-35 0.4552445844261541 1107 36-37 0.4211545657185596 1107 38-39 0.3157560893571443 1107 40-41 0.05691500307762709 1107 42-43 0.36169361453573146 1107 44-45 0.3804075891780059 1107 46-47 0.5594532513237311 1107 48-49 0.4212467236509667 1107 50-51 0.30112493276235597 1107 52-53 0.41881242971969357 1107 54-55 0.4605481944568126 1107 56-57 0.5507241865359873 1107 58-59 0.4711178900637947 1107 60-61 0.5269173991661802 1107 62-63 0.49128310410507936 1107 64-65 0.43236151727273153 1107 66-67 0.4412356312083361 1107 68-69 0.47541550759440554 1107 70-71 0.7978015032219012 1107 72-73 0.5445559027530713 1107 74-75 0.6377394961024443 1107 76-77 0.5877131579005095 1107 78-79 0.5904154315548027 1107 80-81 0.873792426667027 1107 82-83 0.9819294731495738 1107 84-85 0.7237544156251978 1107 86-87 0.6113236232444557 1107 88-89 0.7375566150742436 1107 90-91 0.7524747365837321 1107 92-93 0.712954554261426 1107 94-95 0.6701986147394656 1107 96-97 0.8025639150273349 1107 98-99 1.034108752470896 1107 100-101 1.2103877425841851 1108 1 0.056904161578874124 1108 2 0.12805589023468045 1108 3 0.12495202073739975 1108 4 0.06046954811867522 1108 5 0.24215075870642977 1108 6 0.06728152573814583 1108 7 -0.04002736785754024 1108 8 0.06154530902628608 1108 9 0.14409640242239874 1108 10-11 3.5829347362437147E-4 1108 12-13 0.19683894722053452 1108 14-15 0.21950124203393528 1108 16-17 0.2899644893554978 1108 18-19 0.14467391730356383 1108 20-21 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-2.806702721858347 2316 74-75 -2.3952001639529463 2316 76-77 -2.105499323088548 2316 78-79 -2.963469713374348 2316 80-81 -2.7366616950832707 2316 82-83 -2.3868047215670565 2316 84-85 -2.0699506772998326 2316 86-87 -2.900243502658146 2316 88-89 -3.139749785535887 2316 90-91 -2.933354140421617 2316 92-93 -3.718700835925585 2316 94-95 -4.322979746907716 2316 96-97 -5.278798450223565 2316 98-99 -6.657998752446307 2316 100-101 -6.868608568958859 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5290.0 3 2187.0 4 2175.0 5 2380.0 6 2707.0 7 3039.0 8 3818.0 9 4857.0 10 6088.0 11 7563.0 12 9548.0 13 11210.0 14 13020.0 15 15600.0 16 18406.0 17 22703.0 18 19673.0 19 15312.0 20 15568.0 21 17143.0 22 18997.0 23 21110.0 24 24241.0 25 27880.0 26 32514.0 27 38027.0 28 45017.0 29 54313.0 30 65940.0 31 80645.0 32 100384.0 33 127871.0 34 166591.0 35 226544.0 36 311628.0 37 394562.0 38 297025.0 39 43156.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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26.34351085471617 28.05180018046316 23.26084400047918 44-45 22.707846593430652 25.93147977063354 28.157443144712897 23.20323049122291 46-47 23.302758343279546 26.37757804755279 27.884962424194466 22.434701184973193 48-49 23.101198211299483 26.320204121467043 27.799416544600575 22.7791811226329 50-51 22.494473535726005 26.70641192946963 28.26246028935659 22.536654245447767 52-53 22.93317131747861 27.046990679325766 27.269088390448832 22.75074961274679 54-55 22.464713653579413 26.479059001453 27.724374122100322 23.331853222867267 56-57 23.010090382337093 26.585505102788943 27.260388318907875 23.14401619596609 58-59 23.58783742343763 26.32635380997738 27.253466588438208 22.83234217814678 60-61 22.987563524419887 26.415650957411213 28.229045349808025 22.36774016836087 62-63 22.577435841072706 26.64047919986811 28.021278232675716 22.76080672638347 64-65 22.448902435727312 27.2366112808862 27.54994358699659 22.764542696389896 66-67 22.625974817895873 26.748803026829215 27.13674081697747 23.488481338297447 68-69 22.524842153397 26.713427073990047 27.548804938532157 23.212925834080796 70-71 23.10972831208467 26.487818699748335 27.16997854800387 23.23247444016313 72-73 22.840692877440773 26.295584273535532 27.855688340554835 23.008034508468853 74-75 22.424296778127502 27.42307571410264 27.214692510580175 22.937934997189686 76-77 22.725149461428874 26.623753315716918 27.17510700310628 23.47599021974792 78-79 22.732135716959654 26.78770325263568 27.39901912488847 23.081141905516198 80-81 23.100644183269527 26.730572062177842 27.05185033422613 23.116933420326504 82-83 22.997591249187227 26.70031473347451 27.4831477567931 22.818946260545154 84-85 22.803386327227866 26.962956742305543 27.198537272660722 23.03511965780587 86-87 22.771567078845663 27.19161421558132 27.098171638034536 22.938647067538476 88-89 22.719703018003916 27.006206761932567 26.611920429646148 23.662169790417373 90-91 22.653176065099554 26.525979592644205 27.12013171141308 23.700712630843153 92-93 23.080635016667287 26.863272415977114 27.246478193588498 22.8096143737671 94-95 22.71540900446669 26.64811170960532 27.35991509359511 23.27656419233288 96-97 22.148502863433606 26.640484106250838 27.884185035932507 23.326827994383045 98-99 22.35482027041882 27.084907112234802 26.84744421237771 23.712828404968672 100-101 22.31803879229783 26.575010829388212 27.248148009701374 23.858802368612587 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 200.0 1 156.5 2 192.0 3 266.5 4 238.0 5 253.0 6 409.0 7 820.5 8 1424.5 9 1784.0 10 1702.5 11 1567.5 12 1609.0 13 1778.5 14 2013.5 15 2164.5 16 2326.0 17 2488.5 18 2693.5 19 3030.0 20 3396.5 21 3937.5 22 4784.5 23 5732.5 24 6900.0 25 8708.0 26 11113.5 27 13854.0 28 16599.0 29 20048.0 30 24183.5 31 28733.5 32 33772.5 33 37411.5 34 40550.0 35 44046.0 36 49424.5 37 54478.5 38 56121.5 39 57639.5 40 59909.0 41 62729.0 42 64722.5 43 67241.0 44 71031.0 45 75772.5 46 81269.5 47 94073.5 48 119081.5 49 118182.0 50 94139.0 51 82749.5 52 78967.5 53 77648.5 54 76780.5 55 72798.5 56 66176.0 57 59601.5 58 54712.5 59 48980.5 60 41310.5 61 34941.0 62 29070.0 63 22705.0 64 16408.5 65 12144.5 66 9488.5 67 7593.0 68 6033.0 69 4825.5 70 3863.0 71 2765.0 72 2104.0 73 1838.5 74 1405.0 75 1007.5 76 736.5 77 451.5 78 316.0 79 220.0 80 144.5 81 98.5 82 74.0 83 47.5 84 29.0 85 22.0 86 16.0 87 12.5 88 13.5 89 11.5 90 6.5 91 6.0 92 6.0 93 3.0 94 2.0 95 2.0 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03371809474740663 2 0.023123491313084596 3 0.020793557777735772 4 0.050379317575655794 5 0.05701743010089491 6 0.04879672121202264 7 0.05020347353525211 8 0.0433455559595084 9 0.043081789898902864 10-11 0.051786069898885276 12-13 0.044862210807990174 14-15 0.03980669464638423 16-17 0.04723610535343993 18-19 0.05211577747464219 20-21 0.012001355757551513 22-23 0.019254922424203527 24-25 0.005912755858573914 26-27 0.011056194040381705 28-29 0.006352365959583126 30-31 0.008264669898973204 32-33 0.0030552902020140296 34-35 0.008220708888872282 36-37 0.008968046060587943 38-39 0.01059460343432203 40-41 0.0015825963636331665 42-43 0.0017804209090873124 44-45 0.001868342929289155 46-47 3.5168808080737036E-4 48-49 8.352591919175045E-4 50-51 2.857465656559884E-4 52-53 0.0030113291919131083 54-55 0.006066619393927138 56-57 0.01536437303027199 58-59 0.00916587060604209 60-61 0.00984726626260637 62-63 0.005187399191908712 64-65 6.594151515138194E-5 66-67 0.006660093030289576 68-69 0.016837066868652856 70-71 0.005692950808069307 72-73 0.002417855555550671 74-75 0.003846588383830613 76-77 0.007011781111096945 78-79 0.006220482929280362 80-81 0.010330837373716503 82-83 0.005275321212110554 84-85 0.015891905151483046 86-87 0.0036927248484773883 88-89 0.0055830482828170035 90-91 0.003209153737367254 92-93 0.016595281313097787 94-95 0.010484700909069727 96-97 0.00883616303028518 98-99 0.0216727779797542 100-101 0.036839326464572046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2274743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.784095373080646 #Duplication Level Percentage of deduplicated Percentage of total 1 78.83600307002492 45.554671202307986 2 12.585158198387122 14.544439632418186 3 3.819449504875041 6.621103033870949 4 1.6296340357599552 3.7666771418228633 5 0.8387227963090622 2.4232419026749867 6 0.515474859651741 1.7871749071524938 7 0.31445744763685307 1.2719447401516402 8 0.2283480301231501 1.0555907480712958 9 0.17087106940378008 0.8886267153835479 >10 0.9454677183886065 10.143958295035004 >50 0.07453559710520645 2.9601361710714396 >100 0.03738908456909305 3.901221279221445 >500 0.0023584105208772827 0.8992190776605032 >1k 0.0016737106922353807 2.0942506790841553 >5k 3.803887936898593E-4 1.5209573037837405 >10k+ 7.607775873797185E-5 0.5667871702897209 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12893 0.5667893032311782 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9090 0.3996055818173745 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7672 0.33726886949426815 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6180 0.27167904242369356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6001 0.26381002161562866 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5655 0.2485995121207099 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4047 0.17791020787842848 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3894 0.17118417333298752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3422 0.15043457656535264 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3318 0.14586263151485684 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3130 0.13759796161588364 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2909 0.12788257838358005 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2657 0.11680440383814789 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2405 0.10572622929271572 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2330 0.10242915353514662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.396101010092129E-5 0.0 10-11 0.0 0.0 0.0 4.396101010092129E-5 0.0 12-13 0.0 0.0 0.0 8.792202020184258E-5 1.7584404040368515E-4 14-15 0.0 0.0 0.0 8.792202020184258E-5 2.1980505050460647E-4 16-17 0.0 0.0 0.0 8.792202020184258E-5 2.1980505050460647E-4 18-19 0.0 0.0 0.0 1.0990252525230322E-4 2.1980505050460647E-4 20-21 0.0 0.0 0.0 1.7584404040368515E-4 2.1980505050460647E-4 22-23 0.0 0.0 0.0 3.07727070706449E-4 2.1980505050460647E-4 24-25 0.0 0.0 0.0 3.956490909082916E-4 2.1980505050460647E-4 26-27 0.0 0.0 0.0 6.154541414128981E-4 2.1980505050460647E-4 28-29 0.0 0.0 0.0 0.0011429862626239535 2.1980505050460647E-4 30-31 0.0 0.0 0.0 0.00204418696969284 2.1980505050460647E-4 32-33 0.0 0.0 0.0 0.0039345104040324555 2.1980505050460647E-4 34-35 0.0 0.0 0.0 0.005824833838372071 2.1980505050460647E-4 36-37 0.0 0.0 0.0 0.008308630909074124 2.1980505050460647E-4 38-39 0.0 0.0 0.0 0.01288057595956994 2.637660606055277E-4 40-41 0.0 0.0 0.0 0.017320637979762986 2.637660606055277E-4 42-43 0.0 0.0 0.0 0.0216727779797542 2.637660606055277E-4 44-45 0.0 0.0 0.0 0.024947873232272832 2.637660606055277E-4 46-47 0.0 0.0 0.0 0.029915467373676938 2.637660606055277E-4 48-49 0.0 0.0 0.0 0.0341796853534663 2.637660606055277E-4 50-51 0.0 0.0 0.0 0.03846588383830613 2.637660606055277E-4 52-53 0.0 0.0 0.0 0.04717016383828855 2.637660606055277E-4 54-55 0.0 0.0 0.0 0.06145749212108796 3.0772707070644904E-4 56-57 0.0 0.0 0.0 0.08515247656548455 3.2970757575690964E-4 58-59 0.0 0.0 0.0 0.11629885222198727 3.516880808073703E-4 60-61 0.0 0.0 0.0 0.15228093898959133 3.516880808073703E-4 62-63 0.0 0.0 0.0 0.17456917111075845 3.956490909082916E-4 64-65 0.0 0.0 0.0 0.20241847100969207 4.3961010100921293E-4 66-67 0.0 0.0 0.0 0.2576994412116006 4.3961010100921293E-4 68-69 0.0 0.0 0.0 0.33480705292861657 4.3961010100921293E-4 70-71 0.0 0.0 0.0 0.40830986181735696 4.3961010100921293E-4 72-73 0.0 0.0 0.0 0.49113240484749265 4.3961010100921293E-4 74-75 0.0 0.0 0.0 0.572460273534197 4.3961010100921293E-4 76-77 0.0 0.0 0.0 0.6463147705037449 4.3961010100921293E-4 78-79 0.0 0.0 0.0 0.7190702422207695 4.835711111101342E-4 80-81 0.0 0.0 0.0 0.788045067069115 4.835711111101342E-4 82-83 0.0 0.0 0.0 0.887440910907298 4.835711111101342E-4 84-85 0.0 0.0 0.0 1.0596142069675563 4.835711111101342E-4 86-87 0.0 0.0 0.0 1.2929592485832466 5.714931313119768E-4 88-89 0.0 0.0 0.0 1.5658252382796651 6.594151515138194E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 11060 0.0 23.759512 2 GTACATG 11405 0.0 23.624102 1 GAGTACT 7915 0.0 22.843954 12-13 ACATGGG 11070 0.0 22.836605 3 GTACTTT 8255 0.0 21.672346 14-15 GTGTACG 220 5.6215868E-8 21.599556 1 AGTACTT 8245 0.0 19.970093 12-13 GTATAGG 650 0.0 19.738672 1 GTGTAGC 1380 0.0 19.283083 1 TACCTGG 1720 0.0 18.78659 2 CATGGGG 4830 0.0 18.694427 4 CGTACAC 205 1.5850319E-5 18.54401 3 CATGGGA 5980 0.0 18.357626 4 ACTTTTT 10100 0.0 18.206383 16-17 GTACCTG 1835 0.0 18.127148 1 TATAGCG 135 0.009381492 17.599638 5 AGAGTAC 16300 0.0 16.820784 10-11 TAAGGTG 820 0.0 16.80551 5 TACTTTT 9645 0.0 16.750767 14-15 GTCCTAC 525 0.0 16.292238 1 >>END_MODULE