FastQCFastQC Report
Thu 26 May 2016
SRR936661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274743
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT163460.7185866711096593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87520.38474676040326317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78650.34575334444374595No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69650.3061884353529168No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54380.23905997292880998No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47570.20912252505008258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46290.20349551575716465No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45960.20204480242383424No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC42000.18463624242386942No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC40310.17720683171681373No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33850.14880801919161857No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33300.1463901636360679No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28920.12713524121186437No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26240.11535369050481746No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25590.11249622484825758No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24370.10713298161594519No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22800.10023110303010055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG105300.024.0882342
GTACATG107600.024.0205961
ACATGGG108000.022.8692863
GAGTACT100200.021.6408712-13
GTACTTT101250.021.34607514-15
TATAGGG7750.020.8386362
GTGTAGC14100.020.5545441
AGTACTT101250.020.50161612-13
CATGGGG46200.020.4585344
ACTTTTT119500.018.26357816-17
TACTTTT115550.017.75887514-15
CATGGGA59050.017.534774
CTACACT11000.017.2715284
AGAGTAC185300.017.17554710-11
TACCTGG15700.016.9426162
TGTACGC2002.7618968E-416.6249432
GTACCTG17750.016.5955051
TACACTG19450.016.3616925
TAGCCCT15650.016.0851464
GTATAGT5400.015.8371081
Produced by FastQC (version 0.11.5)