##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936661_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2274743 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.897340490771924 31.0 31.0 34.0 30.0 34.0 2 32.029239786648425 31.0 31.0 34.0 30.0 34.0 3 32.13893393671285 33.0 31.0 34.0 30.0 34.0 4 35.86983408675178 37.0 35.0 37.0 35.0 37.0 5 35.65372483836636 37.0 35.0 37.0 33.0 37.0 6 35.76868463822067 37.0 35.0 37.0 33.0 37.0 7 35.71850798090158 37.0 35.0 37.0 33.0 37.0 8 35.697001375540005 37.0 35.0 37.0 33.0 37.0 9 37.32964251346196 39.0 37.0 39.0 34.0 39.0 10-11 37.199265807170306 39.0 37.0 39.0 33.0 39.0 12-13 37.10620276664221 39.0 37.0 39.0 33.0 39.0 14-15 38.18919477936629 40.0 37.5 41.0 33.0 41.0 16-17 38.12698929065833 40.0 37.0 41.0 33.0 41.0 18-19 38.300699463631716 40.0 38.0 41.0 33.5 41.0 20-21 38.33016455045691 40.0 38.0 41.0 34.0 41.0 22-23 38.232658370637914 40.0 38.0 41.0 33.5 41.0 24-25 38.10403702747959 40.0 37.0 41.0 33.0 41.0 26-27 37.87698038855379 40.0 37.0 41.0 33.0 41.0 28-29 37.80798380300544 40.0 37.0 41.0 33.0 41.0 30-31 37.79357448291961 40.0 37.0 41.0 33.0 41.0 32-33 37.64310012164012 40.0 37.0 41.0 33.0 41.0 34-35 37.459656981030385 40.0 36.0 41.0 32.0 41.0 36-37 37.20848794786927 39.0 36.0 41.0 31.5 41.0 38-39 37.031185500955495 39.0 36.0 41.0 31.0 41.0 40-41 36.82523476278419 39.0 35.5 41.0 31.0 41.0 42-43 36.57606991207358 39.0 35.0 40.5 30.0 41.0 44-45 36.31494392992967 39.0 35.0 40.0 30.0 41.0 46-47 35.949163048309195 39.0 35.0 40.0 29.5 41.0 48-49 35.56953752577763 38.0 35.0 40.0 28.0 41.0 50-51 35.24923123183586 38.0 34.5 40.0 27.0 41.0 52-53 34.979502739430345 38.0 34.0 40.0 26.5 41.0 54-55 34.71839434169047 38.0 34.0 40.0 26.0 41.0 56-57 34.37650231256894 38.0 33.5 40.0 25.5 41.0 58-59 34.036178592482756 37.0 33.0 40.0 24.5 41.0 60-61 33.637295949476496 37.0 33.0 39.5 23.0 41.0 62-63 33.244277045802534 36.0 32.0 39.0 22.5 40.0 64-65 32.81856719638219 36.0 31.5 39.0 22.0 40.0 66-67 32.33818501694478 35.0 31.0 38.5 20.5 40.0 68-69 32.03564314737972 35.0 31.0 38.0 20.0 40.0 70-71 31.579846822256407 35.0 31.0 37.5 18.5 39.5 72-73 31.122369208301773 34.5 30.0 37.0 18.0 39.0 74-75 30.59827483808061 34.0 30.0 36.0 14.0 39.0 76-77 29.19080287311578 32.5 28.0 35.0 12.0 37.0 78-79 29.974541739440454 34.0 29.0 35.0 11.5 37.0 80-81 29.77234790919238 34.0 29.0 35.0 7.5 37.0 82-83 29.428905155439537 34.0 29.0 35.0 7.0 36.5 84-85 29.007937599983823 34.0 29.0 35.0 2.0 36.0 86-87 28.649904626588587 33.5 29.0 35.0 2.0 36.0 88-89 28.238041176519722 33.0 27.5 35.0 2.0 35.0 90-91 27.725374910484394 33.0 27.0 35.0 2.0 35.0 92-93 27.229081483051054 33.0 26.0 34.5 2.0 35.0 94-95 26.620137088013898 32.0 25.0 34.0 2.0 35.0 96-97 25.832868811993265 32.0 24.0 34.0 2.0 35.0 98-99 24.86668142291239 32.0 19.0 34.0 2.0 35.0 100-101 21.589199746960425 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21738755166516555 1101 2 0.31671995198645675 1101 3 0.26215419471336787 1101 4 0.19175664416599858 1101 5 0.11877512518077538 1101 6 0.23610147040089657 1101 7 0.20697078891024745 1101 8 0.1753244464080197 1101 9 0.30490123236876343 1101 10-11 0.2537480099959666 1101 12-13 0.21099506862390882 1101 14-15 0.42318313252339124 1101 16-17 0.38593630946712665 1101 18-19 0.3051936521510825 1101 20-21 0.2072200583498116 1101 22-23 0.23246281422058956 1101 24-25 0.25861994893822526 1101 26-27 0.2570006859673484 1101 28-29 0.22849654334520153 1101 30-31 0.2797185136344993 1101 32-33 0.33708850214202357 1101 34-35 0.37689117639364866 1101 36-37 0.35725393362070434 1101 38-39 0.361177936345932 1101 40-41 0.2359015419453243 1101 42-43 0.3937145179543933 1101 44-45 0.4757710800761501 1101 46-47 0.3690861365971969 1101 48-49 0.38159094840458607 1101 50-51 0.446030781856841 1101 52-53 0.34068033176998824 1101 54-55 0.3598384449102596 1101 56-57 0.4387421504641509 1101 58-59 0.3419083828351859 1101 60-61 0.38009139447616036 1101 62-63 0.3181133789209767 1101 64-65 0.22148177120249102 1101 66-67 0.09064052509801712 1101 68-69 0.15553314674118468 1101 70-71 0.21221069699181072 1101 72-73 0.20698845204233152 1101 74-75 0.3171986044110646 1101 76-77 0.26820206265180957 1101 78-79 -0.05772449481159825 1101 80-81 0.1887674891693969 1101 82-83 0.26212780990340434 1101 84-85 0.2684232052391309 1101 86-87 0.1790968926701204 1101 88-89 0.046881661750443726 1101 90-91 0.018130035938501265 1101 92-93 0.10054520536374056 1101 94-95 0.2825217197194263 1101 96-97 0.3646159182983979 1101 98-99 0.7650589354338493 1101 100-101 1.0241067930398913 1102 1 0.15015445229316882 1102 2 0.250225677882284 1102 3 0.20230934801961098 1102 4 0.11233292787490257 1102 5 0.049694962639215134 1102 6 0.10754587379949498 1102 7 0.1678130497155692 1102 8 0.18825388859494296 1102 9 0.17302092206215747 1102 10-11 0.25190094682640307 1102 12-13 0.22078450342257128 1102 14-15 0.3033087328189197 1102 16-17 0.2671701476643946 1102 18-19 0.17479099238011742 1102 20-21 0.03378082672808347 1102 22-23 0.010999940190295376 1102 24-25 0.14668792086286686 1102 26-27 -0.06198712341573298 1102 28-29 -0.06056884269100493 1102 30-31 0.14137348223442103 1102 32-33 0.14757982094513267 1102 34-35 0.22598611544055558 1102 36-37 0.35282098201375334 1102 38-39 0.34307671728424083 1102 40-41 0.2314685903383733 1102 42-43 0.23154237166698977 1102 44-45 0.2905106441692382 1102 46-47 0.27156120124440974 1102 48-49 0.17139515970861652 1102 50-51 0.1480994926067538 1102 52-53 0.057340841559422984 1102 54-55 0.30608890667604527 1102 56-57 0.19142039206001726 1102 58-59 0.07611599273543845 1102 60-61 0.22918633352307438 1102 62-63 0.012793836992642582 1102 64-65 0.06725199654420067 1102 66-67 0.14771477703742875 1102 68-69 0.08737651578439909 1102 70-71 -0.07722410167830063 1102 72-73 0.1199917767560379 1102 74-75 0.04290972373134494 1102 76-77 -0.038964542446084494 1102 78-79 -0.05587743164203829 1102 80-81 0.2949736214191212 1102 82-83 0.21816770646786665 1102 84-85 0.06561566922139761 1102 86-87 0.07196722883561435 1102 88-89 -0.04288560829019161 1102 90-91 0.026995939152392623 1102 92-93 0.12954409712583725 1102 94-95 0.4461715165424778 1102 96-97 0.5278963024875374 1102 98-99 0.36590858449184793 1102 100-101 0.8015356811078611 1103 1 0.03928275241711532 1103 2 0.06642689973070048 1103 3 -0.01719066962049709 1103 4 0.007752636773354027 1103 5 -0.1327234944295057 1103 6 -0.003936022716374055 1103 7 -0.10266700962107933 1103 8 -0.016112703269442363 1103 9 -0.023598712102653963 1103 10-11 -0.13620697272977367 1103 12-13 -0.08977730716332388 1103 14-15 -0.006797935593908733 1103 16-17 0.11230004404627891 1103 18-19 -0.07621974447247482 1103 20-21 0.026251727761327004 1103 22-23 -0.11462204511913399 1103 24-25 -0.1765510166289701 1103 26-27 -0.018479073418866676 1103 28-29 0.08916160689008024 1103 30-31 0.09140894776916042 1103 32-33 0.12071867657864743 1103 34-35 0.0364565681268445 1103 36-37 0.1402557908423745 1103 38-39 0.2655641039225429 1103 40-41 0.17731449653667397 1103 42-43 0.22050096985929457 1103 44-45 0.17329591933498278 1103 46-47 0.2661911962598822 1103 48-49 0.23414602500039194 1103 50-51 0.2501067993168462 1103 52-53 0.27182386223734056 1103 54-55 0.08294075052006633 1103 56-57 -0.03387196351453525 1103 58-59 0.32192029024948 1103 60-61 0.19026395588946343 1103 62-63 0.18397856705853854 1103 64-65 0.15394645632935777 1103 66-67 0.1925167321925798 1103 68-69 0.2724842309350066 1103 70-71 0.35279238392675083 1103 72-73 0.09368056438934502 1103 74-75 0.06895537357841519 1103 76-77 0.05585184739447513 1103 78-79 0.044112176482681065 1103 80-81 0.17095022775662372 1103 82-83 0.27022818745656707 1103 84-85 0.08421417452105118 1103 86-87 0.07002736635705276 1103 88-89 0.17371491141339135 1103 90-91 0.19734389675079456 1103 92-93 0.3422634504629123 1103 94-95 0.3551815881503231 1103 96-97 0.29889897568419954 1103 98-99 0.5335564391187084 1103 100-101 0.8075193832978584 1104 1 0.12405929097614532 1104 2 0.1910088751111374 1104 3 0.12416025387286567 1104 4 0.030543046555258968 1104 5 -0.08830902350844383 1104 6 0.09912507673125504 1104 7 0.09344650890763972 1104 8 0.037467602787842225 1104 9 0.09465224598977784 1104 10-11 0.08074412217911942 1104 12-13 0.044671522296575006 1104 14-15 0.0855828545371935 1104 16-17 0.1470886872974404 1104 18-19 0.0419609053552179 1104 20-21 0.01039330014162232 1104 22-23 0.10031722291490297 1104 24-25 0.10053093704699023 1104 26-27 0.048308156266131164 1104 28-29 0.13860689033699458 1104 30-31 -0.04657265850711667 1104 32-33 0.03222170645211975 1104 34-35 0.05205119742936404 1104 36-37 0.23138184886434487 1104 38-39 0.07765402278481304 1104 40-41 0.1930266146500017 1104 42-43 0.11538975826714193 1104 44-45 0.18574275938410523 1104 46-47 0.07711305858633466 1104 48-49 0.07820307659911663 1104 50-51 -0.02098898914693592 1104 52-53 0.08703936774308829 1104 54-55 0.05754423823697152 1104 56-57 0.09487869420220107 1104 58-59 -0.05668516097516374 1104 60-61 0.03273259577039056 1104 62-63 -0.02035489849418326 1104 64-65 0.033842861228677634 1104 66-67 -0.11755815749531706 1104 68-69 0.16636892054179953 1104 70-71 0.002456548103097589 1104 72-73 -0.1340568003686471 1104 74-75 -0.03616608537216237 1104 76-77 -0.12823860205324067 1104 78-79 -0.1112461743529991 1104 80-81 0.03703347815040203 1104 82-83 -0.001836688000310005 1104 84-85 -0.10374799735533102 1104 86-87 -0.14339892190313108 1104 88-89 0.019631431592650017 1104 90-91 0.13899828437790873 1104 92-93 0.1247821970800409 1104 94-95 0.18990059206154086 1104 96-97 0.19434944742561555 1104 98-99 0.47059730942686073 1104 100-101 0.8465808192004154 1105 1 0.09383066708755905 1105 2 0.1881486356985036 1105 3 0.10993424129998886 1105 4 0.0871442063644281 1105 5 0.04648784998526878 1105 6 0.026465955061375723 1105 7 0.12359994830593735 1105 8 0.07531461418898289 1105 9 0.01067793540772044 1105 10-11 0.05795130588035846 1105 12-13 -0.05530732149511408 1105 14-15 0.14595822963323712 1105 16-17 0.2098844956006758 1105 18-19 0.0706787668684683 1105 20-21 0.24332684366353163 1105 22-23 0.15906967390622384 1105 24-25 0.12369083508513512 1105 26-27 0.16024579280708195 1105 28-29 0.14837641528017542 1105 30-31 0.14402840382359017 1105 32-33 0.19542201961320416 1105 34-35 0.2031743291755035 1105 36-37 0.09943898784695193 1105 38-39 0.24967367653332673 1105 40-41 0.3205545009464501 1105 42-43 0.2046987082503975 1105 44-45 0.26492448152713166 1105 46-47 0.2540645227696885 1105 48-49 0.3747852880717133 1105 50-51 0.3836564744684594 1105 52-53 0.443971277808906 1105 54-55 0.4846767063900401 1105 56-57 0.4283600389928921 1105 58-59 0.5122667041921432 1105 60-61 0.4468733685039794 1105 62-63 0.49578639668704483 1105 64-65 0.41971272809985294 1105 66-67 0.4995163939999898 1105 68-69 0.6227782138368099 1105 70-71 0.5206698755610724 1105 72-73 0.5269179596290812 1105 74-75 0.31466780902607283 1105 76-77 0.1895730803250366 1105 78-79 0.3818875136645765 1105 80-81 0.6841410816086402 1105 82-83 0.5588056765995724 1105 84-85 0.5601583427574717 1105 86-87 0.7235239152858561 1105 88-89 0.7521854395025471 1105 90-91 0.7562154404023289 1105 92-93 0.657956392471462 1105 94-95 0.7599902184315255 1105 96-97 0.6736693306790151 1105 98-99 0.8941683543882597 1105 100-101 0.9659792479521947 1106 1 0.03060246440389136 1106 2 0.11610496965892025 1106 3 0.12476121741774904 1106 4 0.03100203643728605 1106 5 -0.053684143204328905 1106 6 -0.03699904863228909 1106 7 0.027224936828339708 1106 8 -0.08460955616176591 1106 9 0.026517606625937162 1106 10-11 -0.00929993099247639 1106 12-13 -0.005946142629362328 1106 14-15 0.14019334987266063 1106 16-17 0.1421409925888355 1106 18-19 0.02441666497870898 1106 20-21 0.0042633924912820476 1106 22-23 0.028265112062250353 1106 24-25 -0.02722039886476324 1106 26-27 0.06529308718473459 1106 28-29 -0.02482935879832837 1106 30-31 0.11386052173949679 1106 32-33 0.13547845191018837 1106 34-35 0.10702711993622671 1106 36-37 0.007608939315240093 1106 38-39 0.2804532251249441 1106 40-41 0.3055101378353484 1106 42-43 0.3424468222994861 1106 44-45 0.23730916768413834 1106 46-47 0.03955871947849943 1106 48-49 0.1572567307198014 1106 50-51 0.1705542295915521 1106 52-53 0.07230542686422581 1106 54-55 0.06157554303216983 1106 56-57 0.08310274994617828 1106 58-59 0.1830039818870688 1106 60-61 0.05018168358562747 1106 62-63 0.1497572130039444 1106 64-65 0.06091762029873138 1106 66-67 0.10585337900153746 1106 68-69 0.1482214908294992 1106 70-71 0.28506211935313175 1106 72-73 0.15119157961328966 1106 74-75 0.29912276425801565 1106 76-77 -0.005185751761050739 1106 78-79 0.23520461921300395 1106 80-81 0.36298441949302074 1106 82-83 0.5261076398815838 1106 84-85 0.3499005429471431 1106 86-87 0.4610910707024729 1106 88-89 0.42320134583133395 1106 90-91 0.6534253045892022 1106 92-93 0.6439556369079291 1106 94-95 0.945614714021211 1106 96-97 0.8580105014425392 1106 98-99 1.2032330003974145 1106 100-101 1.4582251397213035 1107 1 0.02982175292819278 1107 2 0.12717583921916997 1107 3 0.05795897353871737 1107 4 0.005069086223826957 1107 5 -0.08224866234318284 1107 6 -0.011962964528713371 1107 7 -0.009816966431984042 1107 8 -0.09267563862774608 1107 9 -0.031523531156310014 1107 10-11 0.0705620925355035 1107 12-13 -0.026740678410703822 1107 14-15 0.06959608655200356 1107 16-17 0.09915425706722658 1107 18-19 0.07947205740999408 1107 20-21 0.11419708914107929 1107 22-23 0.11225443345259833 1107 24-25 -0.009726749286151914 1107 26-27 0.07421414392532455 1107 28-29 0.048811727190376075 1107 30-31 -0.08128497622113429 1107 32-33 -0.15284115232621787 1107 34-35 -0.13536760778588786 1107 36-37 0.016108221441513138 1107 38-39 0.11171957722201142 1107 40-41 -0.14284484596013414 1107 42-43 -0.16651422210204458 1107 44-45 -0.08608627981586636 1107 46-47 0.03346536161940605 1107 48-49 -0.1350702083961366 1107 50-51 -0.020347815872518993 1107 52-53 -0.019801389699040328 1107 54-55 0.03766387387103265 1107 56-57 -0.0817597115418991 1107 58-59 -0.21836264403026462 1107 60-61 -0.1034918495336754 1107 62-63 -0.006679277069594036 1107 64-65 -0.025922250065072205 1107 66-67 0.11276838808444012 1107 68-69 0.1868495713075191 1107 70-71 0.14811675773138333 1107 72-73 0.10833432794618147 1107 74-75 0.00516870817627435 1107 76-77 -0.031168210507111382 1107 78-79 0.0632129290297847 1107 80-81 0.4128633140055733 1107 82-83 0.26179205726136345 1107 84-85 0.13438418799381324 1107 86-87 0.15078206129204474 1107 88-89 0.14648958831650916 1107 90-91 0.1604812225726029 1107 92-93 0.07809976767138949 1107 94-95 0.19983722060013065 1107 96-97 0.39176389563328584 1107 98-99 0.6640574697992356 1107 100-101 1.119719116817894 1108 1 -0.059143782867764116 1108 2 0.016242893305459916 1108 3 -0.025155091049157363 1108 4 -0.0864457065694495 1108 5 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-0.47872059128948763 2316 90-91 -0.7899665659142379 2316 92-93 -0.6360715783254953 2316 94-95 -0.7938027556724307 2316 96-97 -0.6348915614543991 2316 98-99 -0.7154220875859281 2316 100-101 -0.942649033110964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 32.0 5 54.0 6 156.0 7 364.0 8 906.0 9 1747.0 10 2824.0 11 4552.0 12 6288.0 13 8746.0 14 11365.0 15 14959.0 16 19045.0 17 24240.0 18 21491.0 19 15007.0 20 14003.0 21 14466.0 22 15439.0 23 17250.0 24 20293.0 25 24240.0 26 29712.0 27 36680.0 28 45726.0 29 56312.0 30 69492.0 31 86265.0 32 107747.0 33 137183.0 34 179815.0 35 243825.0 36 345282.0 37 436944.0 38 249033.0 39 13249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.965108941108696 19.15037149258884 14.994096137312631 21.890423428989834 2 15.89205206155767 21.06347667627269 38.91339853118001 24.13107273098964 3 20.911041259094805 24.289231310311475 29.43490207284637 25.364825357747346 4 13.254253024749268 16.74160583171483 36.9006222265988 33.103518916937105 5 13.694728410768436 38.03787891443438 33.521239078885245 14.74615359591194 6 31.93623532194243 36.296032204134185 16.77383647090941 14.993896003013973 7 28.91438725165876 32.918971505792086 19.878465391474993 18.288175851074165 8 26.732118749238925 33.75071381690151 18.941436461173854 20.575730972685705 9 26.10814223188207 17.45740552193952 20.18944849185038 36.245003754328025 10-11 25.56972058485093 26.392344421885028 26.43450320663937 21.603431786624675 12-13 27.316171209756284 23.667873629198514 26.8078885365376 22.208066624507605 14-15 23.379733836024986 25.34472859574588 26.336688811828257 24.938848756400876 16-17 22.177468723657036 28.39695210210656 26.806004794075655 22.61957438016075 18-19 21.890538843289107 27.378961051863882 29.134587951254275 21.595912153592735 20-21 23.333910687932658 25.679032752271358 28.90970540408301 22.077351155712975 22-23 22.558591882477508 25.78891293849531 29.086715524466673 22.565779654560508 24-25 23.00696524674224 25.876788460304162 29.26819189333905 21.848054399614547 26-27 22.654461588647283 25.58733805586586 29.82403268755712 21.93416766792973 28-29 21.943908634183927 26.920646225642614 29.32391186553196 21.8115332746415 30-31 22.45863273890735 25.912405309587065 29.05374320436995 22.57521874713563 32-33 22.425916547531756 26.00099704053049 29.181943191549596 22.39114322038816 34-35 23.207440492023178 25.84337314967024 29.035399447885208 21.913786910421376 36-37 23.066445544972353 25.51937511431265 29.467799886039085 21.946379454675917 38-39 22.49534848548798 26.13696116293139 29.45852941797155 21.909160933609083 40-41 22.72024882402075 26.23477821251154 29.034202312392843 22.010770651074868 42-43 22.38384795760735 26.418275719270913 28.35179849599997 22.846077827121764 44-45 22.80393754129631 25.919465217462022 28.411553251192785 22.865043990048882 46-47 23.50077503294879 26.622645557166486 27.900724565687007 21.975854844197716 48-49 23.427319538002518 26.543336332955413 27.77345980689585 22.255884322146223 50-51 22.65493424913715 26.923124266119437 28.225041026973397 22.19690045777002 52-53 23.310082540935806 27.28996638881922 27.19162329627846 22.208327773966513 54-55 22.713944126425318 26.74679107440153 27.52987135282421 23.009393446348945 56-57 23.218685397197675 26.78611739699545 27.233052016349284 22.76214518945759 58-59 23.872748601974173 26.451015737942885 27.124591516646735 22.55164414343621 60-61 23.103253324235993 26.7292929310692 28.134075540147137 22.033378204547674 62-63 22.8079191745826 26.734094453122285 27.98405543580475 22.47393093649036 64-65 22.67302488793866 27.45905702504973 27.45043988102186 22.41747820598975 66-67 22.91723314015534 26.85210728137787 27.097065621534526 23.13359395693226 68-69 22.800708937843964 26.891521211533714 27.363743452073606 22.944026398548715 70-71 23.34909921692253 26.789795594491334 27.05868311277362 22.80242207581252 72-73 23.16477493283792 26.480917879599303 27.65024809635995 22.704059091202833 74-75 22.658128929936264 27.758635281032003 26.930997985366705 22.652237803665027 76-77 23.078079622698244 26.859911600674906 26.91301801837447 23.148990758252378 78-79 23.077905472047366 27.037287542470622 27.113716530444876 22.771090455037136 80-81 23.43967136676554 26.983407872830472 26.875961968370284 22.700958792033703 82-83 23.28921927659571 26.981615503856883 27.19237468533399 22.536790534213416 84-85 22.96028444405366 27.327819573663646 26.971714962438657 22.740181019844037 86-87 23.02229594291697 27.334165255644656 26.699518585594323 22.944020215844056 88-89 22.93120359741361 27.347712025774197 26.2976676868073 23.423416690004895 90-91 23.013925570544778 26.733112063625082 26.75634846066014 23.49661390517 92-93 23.324458953722953 27.21196036598903 26.837583466573584 22.625997213714435 94-95 22.98849336700242 27.025354686005286 26.945079940633615 23.04107200635867 96-97 22.428399319211866 26.968239778451515 27.441265757732886 23.162095144603732 98-99 22.658611268032875 27.385812855959006 26.41061431023909 23.544961565769025 100-101 22.381020000650658 27.123250151893103 26.697465869114307 23.798263978341936 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 348.0 1 263.5 2 251.5 3 335.0 4 291.5 5 264.0 6 444.5 7 937.5 8 1536.5 9 1924.0 10 1916.5 11 1787.5 12 1870.0 13 2015.5 14 2252.0 15 2512.5 16 2730.0 17 2964.0 18 3268.0 19 3621.5 20 4019.0 21 4557.5 22 5266.5 23 6237.0 24 7463.5 25 9118.5 26 11425.5 27 14239.5 28 17079.0 29 20709.5 30 25070.5 31 29198.5 32 34001.5 33 37749.0 34 40741.5 35 44109.0 36 49252.5 37 54213.5 38 56075.5 39 57932.5 40 60248.0 41 62455.0 42 64277.0 43 66922.0 44 70747.0 45 74949.5 46 79471.5 47 93174.0 48 120853.5 49 119178.5 50 92531.0 51 81134.5 52 78065.0 53 77022.5 54 76382.0 55 72536.5 56 65222.0 57 58581.5 58 54511.5 59 49118.0 60 41146.5 61 34490.0 62 28575.0 63 22303.0 64 16035.0 65 11900.5 66 9340.5 67 7447.0 68 5997.0 69 4779.0 70 3716.5 71 2752.5 72 2206.0 73 1867.0 74 1433.5 75 1074.0 76 782.5 77 517.5 78 360.5 79 244.0 80 186.5 81 141.0 82 101.0 83 65.0 84 37.0 85 21.0 86 13.0 87 6.0 88 1.5 89 1.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0355204961615444 2 0.005627009292917926 3 0.00408837393938568 4 3.956490909082916E-4 5 9.231812121193471E-4 6 1.7584404040368518E-4 7 0.0 8 0.0 9 0.0015386353535322451 10-11 4.176295959587523E-4 12-13 0.0010770447474725718 14-15 0.002417855555550671 16-17 0.0026596411111057382 18-19 0.0 20-21 0.0 22-23 0.00204418696969284 24-25 9.231812121193471E-4 26-27 0.00509947717170687 28-29 0.006616132020188654 30-31 0.0012089277777753355 32-33 4.835711111101342E-4 34-35 0.009495578181798999 36-37 0.01226512181815704 38-39 0.009451617171698078 40-41 0.0018903234343396154 42-43 3.956490909082916E-4 44-45 0.0010550642424221111 46-47 4.3961010100921294E-5 48-49 5.275321212110556E-4 50-51 8.792202020184259E-4 52-53 0.002351914040399289 54-55 0.003165192727266333 56-57 0.0011210057575734929 58-59 7.693176767661226E-4 60-61 0.00373668585857831 62-63 0.004923633131303185 64-65 0.009121909595941167 66-67 0.003099251212114951 68-69 0.0031871732323167936 70-71 0.0 72-73 9.671422222202684E-4 74-75 0.005956716868674835 76-77 0.002857465656559884 78-79 0.0036267833333260067 80-81 0.004835711111101342 82-83 0.004791750101000421 84-85 0.0057369118181702285 86-87 0.004022432424234298 88-89 0.009671422222202684 90-91 0.01292453696967086 92-93 0.0013188303030276386 94-95 0.002417855555550671 96-97 0.0011649667676744142 98-99 0.003428958787871861 100-101 0.005143438181807791 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2274743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.24881146830123 #Duplication Level Percentage of deduplicated Percentage of total 1 76.83679019818415 42.45141335488891 2 13.634454477969907 15.065748098529893 3 4.267524817965367 7.073270223121954 4 1.8177327486519286 4.017102957201096 5 0.9348940948213967 2.582589379380774 6 0.5601344281672754 1.856805685123111 7 0.3610006840160082 1.3961401111789753 8 0.24466302778167384 1.0813873196138752 9 0.1888982854285828 0.9392765182496212 >10 1.0259895499217777 10.548038267273864 >50 0.08013733336519273 3.0320879627600084 >100 0.0424665474868867 4.266837783955149 >500 0.002934490013158616 1.0879165617866278 >1k 0.001982763522404301 2.6135324362719157 >5k 3.172421635846882E-4 1.2716021678365492 >10k+ 7.931054089617205E-5 0.7162511728275753 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16346 0.7185866711096593 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8752 0.38474676040326317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7865 0.34575334444374595 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6965 0.3061884353529168 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5438 0.23905997292880998 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4757 0.20912252505008258 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4629 0.20349551575716465 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4596 0.20204480242383424 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4200 0.18463624242386942 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4031 0.17720683171681373 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3385 0.14880801919161857 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3330 0.1463901636360679 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2892 0.12713524121186437 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2624 0.11535369050481746 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2559 0.11249622484825758 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2437 0.10713298161594519 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2280 0.10023110303010055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.396101010092129E-5 0.0 10-11 0.0 0.0 0.0 4.396101010092129E-5 0.0 12-13 0.0 0.0 0.0 1.0990252525230322E-4 2.1980505050460644E-5 14-15 0.0 0.0 0.0 1.3188303030276386E-4 6.594151515138193E-5 16-17 0.0 0.0 0.0 1.3188303030276386E-4 8.792202020184258E-5 18-19 0.0 0.0 0.0 1.978245454541458E-4 1.3188303030276386E-4 20-21 0.0 0.0 0.0 2.637660606055277E-4 2.1980505050460647E-4 22-23 0.0 0.0 0.0 4.176295959587523E-4 2.1980505050460647E-4 24-25 0.0 0.0 0.0 5.714931313119768E-4 2.637660606055277E-4 26-27 0.0 0.0 0.0 8.352591919175045E-4 2.637660606055277E-4 28-29 0.0 0.0 0.0 0.0013627913131285599 2.637660606055277E-4 30-31 0.0 0.0 0.0 0.002285972525247907 2.857465656559884E-4 32-33 0.0 0.0 0.0 0.004242237474738905 3.516880808073703E-4 34-35 0.0 0.0 0.0 0.006176521919179442 3.516880808073703E-4 36-37 0.0 0.0 0.0 0.008682299494931954 3.516880808073703E-4 38-39 0.0 0.0 0.0 0.013408108080780993 3.516880808073703E-4 40-41 0.0 0.0 0.0 0.017870150606024503 3.516880808073703E-4 42-43 0.0 0.0 0.0 0.02220031010096525 3.516880808073703E-4 44-45 0.0 0.0 0.0 0.02545342484843343 3.516880808073703E-4 46-47 0.0 0.0 0.0 0.03061884353529168 3.956490909082916E-4 48-49 0.0 0.0 0.0 0.03475117848477828 4.3961010100921293E-4 50-51 0.0 0.0 0.0 0.03903737696961811 4.3961010100921293E-4 52-53 0.0 0.0 0.0 0.04776363747465098 4.835711111101342E-4 54-55 0.0 0.0 0.0 0.062138887777652245 5.714931313119768E-4 56-57 0.0 0.0 0.0 0.08598773575740204 5.714931313119768E-4 58-59 0.0 0.0 0.0 0.11724401393915708 5.714931313119768E-4 60-61 0.0 0.0 0.0 0.15307223717140794 6.154541414128981E-4 62-63 0.0 0.0 0.0 0.17525056676732273 6.154541414128981E-4 64-65 0.0 0.0 0.0 0.2029020421208022 6.154541414128981E-4 66-67 0.0 0.0 0.0 0.25851271989846764 6.374346464633587E-4 68-69 0.0 0.0 0.0 0.33669737636295616 6.594151515138194E-4 70-71 0.0 0.0 0.0 0.40991443868604055 6.594151515138194E-4 72-73 0.0 0.0 0.0 0.49267104020102487 6.594151515138194E-4 74-75 0.0 0.0 0.0 0.5747682265644953 6.594151515138194E-4 76-77 0.0 0.0 0.0 0.6498975928269699 6.594151515138194E-4 78-79 0.0 0.0 0.0 0.722477220503591 6.594151515138194E-4 80-81 0.0 0.0 0.0 0.791188279291331 6.594151515138194E-4 82-83 0.0 0.0 0.0 0.8902763960588076 7.033761616147406E-4 84-85 0.0 0.0 0.0 1.062032062523107 7.033761616147406E-4 86-87 0.0 0.0 0.0 1.2948056110074853 7.912981818165832E-4 88-89 0.0 0.0 0.0 1.5680892302998624 8.792202020184259E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 10530 0.0 24.088234 2 GTACATG 10760 0.0 24.020596 1 ACATGGG 10800 0.0 22.869286 3 GAGTACT 10020 0.0 21.64087 12-13 GTACTTT 10125 0.0 21.346075 14-15 TATAGGG 775 0.0 20.838636 2 GTGTAGC 1410 0.0 20.554544 1 AGTACTT 10125 0.0 20.501616 12-13 CATGGGG 4620 0.0 20.458534 4 ACTTTTT 11950 0.0 18.263578 16-17 TACTTTT 11555 0.0 17.758875 14-15 CATGGGA 5905 0.0 17.53477 4 CTACACT 1100 0.0 17.271528 4 AGAGTAC 18530 0.0 17.175547 10-11 TACCTGG 1570 0.0 16.942616 2 TGTACGC 200 2.7618968E-4 16.624943 2 GTACCTG 1775 0.0 16.595505 1 TACACTG 1945 0.0 16.361692 5 TAGCCCT 1565 0.0 16.085146 4 GTATAGT 540 0.0 15.837108 1 >>END_MODULE