FastQCFastQC Report
Thu 26 May 2016
SRR936660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319426
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129900.5600523577816235No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT90190.38884620591473923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74670.32193309896500255No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61080.26334101626868023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.2554080190529899No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56780.24480194668853417No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40930.17646607393380948No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38980.16805882144978973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34610.14921795306252497No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34120.14710536141269434No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30220.13029085644465485No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29150.12567764610726964No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28100.12115066400048978No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24190.10429304491714761No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23560.1015768556530797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118000.024.8455261
TACATGG115000.024.8325732
ACATGGG115800.023.7603343
GAGTACT81450.022.49427812-13
GTATAGG5300.022.4134751
GTACTTT84000.021.69451114-15
AGTACTT84350.021.10111812-13
CATGGGG48800.020.4507924
TACCTGG17050.020.0656472
CATGGGA61200.020.0345174
GTATAGC8750.019.5496731
ATACCGT1951.0287489E-519.497346
ACTTTTT101250.018.39594316-17
TACTTTT98750.018.09316414-15
GTACCTG20450.017.8913251
AGAGTAC169800.017.48081210-11
TAAGGTG8700.017.4803735
GTGTACG2253.5295263E-516.8947811
GTATAGA6000.016.6307981
ATGGGAG27150.016.6292935
Produced by FastQC (version 0.11.5)