FastQCFastQC Report
Thu 26 May 2016
SRR936660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319426
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT165990.715651199908943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89380.38535396257522336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80530.34719797053236445No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT71480.3081796961834523No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57120.24626782660882476No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47640.20539564530189797No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47040.20280879838373805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.1984973868534715No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43740.18858114033385845No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC42070.18138108307831333No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34200.14745027433511565No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34010.14663110614436503No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29090.12541896141545364No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27210.11731350773855256No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26310.11343323736131267No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24830.10705234829651818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT7850.024.1985154
TAAGGTG9000.022.689475
GTACATG114500.022.3654841
GAGTACT103050.022.30466812-13
TACATGG112800.022.0248372
ACATGGG111800.021.4108523
GTACTTT106800.021.1435414-15
AGTACTT106600.020.53724312-13
TACCTGG16600.020.3175052
ACTTTTT122600.018.4961516-17
TACTTTT118750.018.25599714-15
GTACCTG19300.017.4781741
CCTACAC9550.017.4064353
CATGGGA57450.017.1937944
AGAGTAC196850.017.12857810-11
ACCGTCG2253.546761E-516.8851878
CATGGGG50600.016.5181184
TATACAG14200.016.3872535
GTATATA11450.016.182821
GTGTAGC13100.015.9579261
Produced by FastQC (version 0.11.5)