FastQCFastQC Report
Thu 26 May 2016
SRR936659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219368
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60710.27354634292285007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.21898125952974No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39070.17604110719808522No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28330.12764895231435255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.12449490125116701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG83650.024.7026351
GTATCAA133550.024.2583071
TACATGG84350.023.8223742
ACGACGG1601.8573228E-623.755196
ACATGGG83800.022.8465273
GAGTACT70650.021.45002712-13
TCAACGC150250.021.3426554
ATCAACG151250.021.2015463
CAACGCA152450.021.0662965
GGTATCA99650.020.8316731
TATCAAC153950.020.7665862
GTGTAGC14900.020.7227341
AACGCAG155050.020.6526896
TAGATCG1404.848021E-420.3606745
AGTACTT76300.020.23523112-13
GTACTTT75000.020.17584814-15
GTATAGG6150.020.0825211
TAAGGTG8900.019.7506165
TAGACCG1456.1413314E-419.6585835
CATGGGG47850.019.161724
Produced by FastQC (version 0.11.5)