##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936659_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219368 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.372291120715445 31.0 31.0 34.0 30.0 34.0 2 31.457509975812933 31.0 31.0 34.0 28.0 34.0 3 31.47064389501876 33.0 31.0 34.0 28.0 34.0 4 35.09593046308679 37.0 35.0 37.0 32.0 37.0 5 35.10078995461771 37.0 35.0 37.0 32.0 37.0 6 35.08447540020402 37.0 35.0 37.0 32.0 37.0 7 34.98285187494818 37.0 35.0 37.0 32.0 37.0 8 34.94377903979872 37.0 35.0 37.0 32.0 37.0 9 36.44165591285447 39.0 37.0 39.0 32.0 39.0 10-11 36.30839432667318 39.0 37.0 39.0 32.0 39.0 12-13 36.20716595895769 39.0 35.0 39.0 32.0 39.0 14-15 37.30650009372037 40.0 37.0 41.0 32.0 41.0 16-17 37.19466330054322 39.5 36.5 41.0 31.0 41.0 18-19 37.145325606208615 39.0 36.0 41.0 31.0 41.0 20-21 36.99005054592118 39.0 36.0 41.0 31.0 41.0 22-23 37.10822585528854 39.0 36.0 41.0 31.0 41.0 24-25 37.15112793371807 39.0 37.0 41.0 31.0 41.0 26-27 37.05185282476813 39.0 36.0 41.0 31.0 41.0 28-29 36.91383605603036 39.0 36.0 41.0 30.0 41.0 30-31 36.69897150900617 39.0 36.0 41.0 30.0 41.0 32-33 36.50654781000718 39.0 35.5 41.0 30.0 41.0 34-35 36.31597080790567 39.0 35.0 40.5 30.0 41.0 36-37 36.11214589018135 39.0 35.0 40.0 29.5 41.0 38-39 35.89198005918802 38.0 35.0 40.0 28.5 41.0 40-41 35.66741455225091 38.0 35.0 40.0 27.5 41.0 42-43 35.42684043385324 38.0 34.5 40.0 27.0 41.0 44-45 35.157612662703976 38.0 34.0 40.0 26.0 41.0 46-47 34.995453660681775 38.0 34.0 40.0 25.5 41.0 48-49 34.8287287191669 38.0 34.0 40.0 24.0 41.0 50-51 33.862829868683335 37.5 33.0 39.5 23.5 40.5 52-53 33.968510404763876 37.5 33.0 39.5 23.5 40.5 54-55 34.3969720208636 38.0 34.0 40.0 24.0 41.0 56-57 34.26321254519305 38.0 34.0 40.0 23.0 41.0 58-59 34.01239429423151 38.0 33.0 40.0 23.0 41.0 60-61 33.70348495607759 37.0 33.0 40.0 22.0 41.0 62-63 33.318246681037124 37.0 32.5 40.0 21.5 41.0 64-65 32.94710566251293 36.0 32.0 39.0 20.0 41.0 66-67 32.469304775053075 35.5 31.5 39.0 18.5 40.5 68-69 32.06423315105922 35.0 31.0 38.5 18.0 40.0 70-71 31.66309417816243 35.0 31.0 38.0 14.5 40.0 72-73 31.264966873452263 35.0 31.0 37.0 12.5 39.5 74-75 30.833383647957437 35.0 30.0 37.0 9.0 39.0 76-77 30.368840588852322 34.0 30.0 36.0 7.0 39.0 78-79 29.87833721131421 34.0 29.0 36.0 4.5 38.0 80-81 29.425258902534416 34.0 29.0 35.0 2.0 37.0 82-83 29.012273539133663 34.0 29.0 35.0 2.0 37.0 84-85 28.630107084539382 34.0 28.5 35.0 2.0 36.0 86-87 28.260135543091547 34.0 27.5 35.0 2.0 36.0 88-89 27.8416495146366 33.5 27.0 35.0 2.0 35.5 90-91 27.429682233861172 33.0 26.0 35.0 2.0 35.0 92-93 26.959928457110312 33.0 25.5 35.0 2.0 35.0 94-95 26.435523311140827 33.0 25.0 35.0 2.0 35.0 96-97 25.819402866041145 32.5 23.5 35.0 2.0 35.0 98-99 25.019914678412952 32.0 18.0 35.0 2.0 35.0 100-101 22.069597741338974 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4239311230953824 1101 2 0.3948996552681834 1101 3 0.4003461361838525 1101 4 0.5231471494883522 1101 5 0.3967391466571897 1101 6 0.4875937510364565 1101 7 0.49818184139003563 1101 8 0.4901168855894582 1101 9 0.5429009316878037 1101 10-11 0.7577692074628146 1101 12-13 0.6867117534088365 1101 14-15 0.7339922059513029 1101 16-17 0.797478322624265 1101 18-19 0.8249500264262863 1101 20-21 0.7817765637407987 1101 22-23 0.7525731876681832 1101 24-25 0.6931373290794554 1101 26-27 0.6653849531899354 1101 28-29 0.579566063879362 1101 30-31 0.5790759148634379 1101 32-33 0.5123697377584406 1101 34-35 0.3636755379848893 1101 36-37 0.3335336849076569 1101 38-39 0.4295470741653773 1101 40-41 0.4352432982553296 1101 42-43 0.5347545802321534 1101 44-45 0.40697679403939446 1101 46-47 0.5219025594644577 1101 48-49 0.4628224381041619 1101 50-51 0.4048047560483852 1101 52-53 0.47499442423005434 1101 54-55 0.404660168433729 1101 56-57 0.43322490165628125 1101 58-59 0.4623206005108855 1101 60-61 0.46264560017641543 1101 62-63 0.37819122730289934 1101 64-65 0.34888701227338714 1101 66-67 0.505254840895212 1101 68-69 0.5202552099988722 1101 70-71 0.45920215191559066 1101 72-73 0.2854613951272853 1101 74-75 0.39869533841889293 1101 76-77 0.36668712414970983 1101 78-79 0.2852347442915928 1101 80-81 0.2737618204156398 1101 82-83 0.22595183011220854 1101 84-85 0.20686328047301572 1101 86-87 0.2319838875640201 1101 88-89 0.32338966922575096 1101 90-91 0.4570901737392141 1101 92-93 0.298185311221534 1101 94-95 0.28212944202816104 1101 96-97 0.28443729272996876 1101 98-99 0.2105221110141251 1101 100-101 0.5666521912952014 1102 1 0.3027242215818404 1102 2 0.3540963270658537 1102 3 0.28178503479427164 1102 4 0.3014778962632292 1102 5 0.265231355044655 1102 6 0.31356153452080804 1102 7 0.2864119826224467 1102 8 0.4134410448877688 1102 9 0.4792224595050598 1102 10-11 0.5051930724479661 1102 12-13 0.5568197711785317 1102 14-15 0.5512429715655784 1102 16-17 0.3710879860347518 1102 18-19 0.3885113489192378 1102 20-21 0.30100911745663694 1102 22-23 0.20098822747029743 1102 24-25 0.34895954231715365 1102 26-27 0.03953467035309899 1102 28-29 -0.08507136520085368 1102 30-31 0.17971353486466057 1102 32-33 0.17506321198024466 1102 34-35 0.12650188480272107 1102 36-37 -0.10081967196791908 1102 38-39 -0.10989741590357482 1102 40-41 -0.099473721018569 1102 42-43 -0.011545026765382715 1102 44-45 0.007389169370426885 1102 46-47 -0.04449429090049506 1102 48-49 0.2343530981222841 1102 50-51 0.24402161493460284 1102 52-53 0.3531290558262228 1102 54-55 0.22674221813917228 1102 56-57 0.2177088499026496 1102 58-59 0.17546487993500648 1102 60-61 0.04330572358718854 1102 62-63 -0.027333830026044836 1102 64-65 0.06023852942855257 1102 66-67 0.18102254337851775 1102 68-69 0.17260245038315958 1102 70-71 0.1949815202337959 1102 72-73 -0.03510093742200837 1102 74-75 -0.003986119010281897 1102 76-77 0.05313952410394407 1102 78-79 0.05400098047351065 1102 80-81 0.13523290886891814 1102 82-83 0.14786184033911454 1102 84-85 -0.07600148919693694 1102 86-87 -0.026723128010779362 1102 88-89 -0.017407692695194044 1102 90-91 0.1433649188912618 1102 92-93 0.15748565593221642 1102 94-95 -0.009161612129531704 1102 96-97 0.04430962834663177 1102 98-99 -0.010042608047722723 1102 100-101 0.07840864347755883 1103 1 0.3062433234678714 1103 2 0.14370093273446827 1103 3 0.21909314088269483 1103 4 0.3907198119705555 1103 5 0.3818620533463033 1103 6 0.3354668056578589 1103 7 0.4076547916336466 1103 8 0.40967671391009475 1103 9 0.20354802581891818 1103 10-11 0.4641184880167941 1103 12-13 0.08236675874743327 1103 14-15 0.4113777831450349 1103 16-17 0.4493834935322738 1103 18-19 0.5440312582145381 1103 20-21 0.5928104775976735 1103 22-23 0.3629356299101332 1103 24-25 0.4049364109005609 1103 26-27 0.22681067333364524 1103 28-29 0.3547487285531048 1103 30-31 0.2640141487963348 1103 32-33 0.20585213243540323 1103 34-35 0.5685651161147405 1103 36-37 0.39623629051331477 1103 38-39 0.37061095130444244 1103 40-41 0.20943247563283762 1103 42-43 0.3163677129074003 1103 44-45 0.4957661264252806 1103 46-47 0.5126346390460057 1103 48-49 0.5631037396476017 1103 50-51 0.47107884436123726 1103 52-53 0.650528811456077 1103 54-55 0.5185854518991917 1103 56-57 0.5307143664835223 1103 58-59 0.36141789129241886 1103 60-61 0.3690627234604946 1103 62-63 0.48862195143231446 1103 64-65 0.5561301905657459 1103 66-67 0.6485801518150822 1103 68-69 0.5034373244509354 1103 70-71 0.44651957046925617 1103 72-73 0.30589312081882625 1103 74-75 0.32549935084393056 1103 76-77 0.3244112151754166 1103 78-79 0.19976841654108313 1103 80-81 0.29630483056740076 1103 82-83 0.037849750673739635 1103 84-85 0.24462309340897548 1103 86-87 0.3298406154478073 1103 88-89 0.09359540595260896 1103 90-91 0.005267011927998055 1103 92-93 -0.13164846204831449 1103 94-95 -0.065968007841672 1103 96-97 0.09751725492843377 1103 98-99 0.0746535505308934 1103 100-101 0.3722542951706522 1104 1 0.31248258342900925 1104 2 0.3373142788443211 1104 3 0.23654352926391553 1104 4 0.3078477538266853 1104 5 0.32329214918804894 1104 6 0.4456468903469144 1104 7 0.4466557427349045 1104 8 0.46910269842480545 1104 9 0.4766363212063425 1104 10-11 0.5710812451754848 1104 12-13 0.4912664702050833 1104 14-15 0.36518490944521176 1104 16-17 0.3837958717672265 1104 18-19 0.3447273644095432 1104 20-21 0.23922163171355493 1104 22-23 0.34050110511279996 1104 24-25 0.4215133207315205 1104 26-27 0.16746231152972513 1104 28-29 0.12600062227958375 1104 30-31 0.3603048984889696 1104 32-33 0.285156445581471 1104 34-35 0.30411123203489154 1104 36-37 0.14602059752095187 1104 38-39 0.07773354879232386 1104 40-41 0.004016239459943449 1104 42-43 0.19683074576054338 1104 44-45 0.2572107921390625 1104 46-47 -0.05607952736637145 1104 48-49 0.19907419305345542 1104 50-51 0.16996986564412708 1104 52-53 0.1133569012414597 1104 54-55 0.12798953253401635 1104 56-57 0.228422756695565 1104 58-59 0.3127642884387569 1104 60-61 0.47303968289860876 1104 62-63 0.5701489953632048 1104 64-65 0.35920672655859676 1104 66-67 0.3944054775181911 1104 68-69 0.3879084104372694 1104 70-71 0.18252495409496916 1104 72-73 0.0676781665805386 1104 74-75 -0.0024496876158899283 1104 76-77 0.1484115328039799 1104 78-79 0.23347897840459808 1104 80-81 0.4177665286668031 1104 82-83 0.2157618960405543 1104 84-85 0.12832598222291836 1104 86-87 0.15189746560742634 1104 88-89 0.3157359904962327 1104 90-91 0.19744969117846978 1104 92-93 -0.0722429984801316 1104 94-95 0.049421051460296184 1104 96-97 0.3361301370864531 1104 98-99 0.47929984971201733 1104 100-101 0.48754699524542033 1105 1 0.4420028216721512 1105 2 0.2561680411759504 1105 3 0.3080192964572923 1105 4 0.29185600900688513 1105 5 0.31122029724416933 1105 6 0.28425579585157834 1105 7 0.4301463235717975 1105 8 0.504701123690829 1105 9 0.5577574595126151 1105 10-11 0.5516825756926735 1105 12-13 0.39768742039162674 1105 14-15 0.5392369065387115 1105 16-17 0.48394671893344565 1105 18-19 0.4991574645685475 1105 20-21 0.3800309355451006 1105 22-23 0.12252428311873587 1105 24-25 0.25163926933053204 1105 26-27 0.06894574657822972 1105 28-29 0.10306906071022581 1105 30-31 0.03474028833853282 1105 32-33 -0.1362187045440848 1105 34-35 -0.011955308883976556 1105 36-37 -0.0011816707083980305 1105 38-39 -0.04560110853235955 1105 40-41 -0.028538731280477236 1105 42-43 -0.14487526415621943 1105 44-45 0.16319783710331137 1105 46-47 0.2181076297920228 1105 48-49 0.37387650420271257 1105 50-51 0.36042429586978386 1105 52-53 0.33100696091518955 1105 54-55 0.18938869019942928 1105 56-57 0.08080227034523091 1105 58-59 0.06764815369032107 1105 60-61 0.078385470535423 1105 62-63 0.08518163080876917 1105 64-65 0.1741409725047376 1105 66-67 0.3308771095916683 1105 68-69 0.25602224357420056 1105 70-71 0.16964160840019815 1105 72-73 0.059417809642507535 1105 74-75 0.14636772456458047 1105 76-77 0.09456806748564972 1105 78-79 2.014284035354308E-4 1105 80-81 0.16505642504408868 1105 82-83 0.030845483191185963 1105 84-85 -0.09267170487086673 1105 86-87 -0.21939065248005107 1105 88-89 -0.17561382809812542 1105 90-91 -0.17481589019178045 1105 92-93 -0.2716878633388937 1105 94-95 -0.07780432073187526 1105 96-97 0.02215264744935297 1105 98-99 0.28367950015320176 1105 100-101 0.5381098170662995 1106 1 0.22673911498475618 1106 2 0.1858670031630929 1106 3 0.3229589684640004 1106 4 0.25942703442972714 1106 5 0.12621955245412408 1106 6 0.3554743879875488 1106 7 0.48350661898476943 1106 8 0.4583153278911709 1106 9 0.46614760434196967 1106 10-11 0.569964280452453 1106 12-13 0.5669288840434987 1106 14-15 0.5445876618374328 1106 16-17 0.5114374634718217 1106 18-19 0.437393624907223 1106 20-21 0.26314379062119997 1106 22-23 0.38495122397696235 1106 24-25 0.3103006673728643 1106 26-27 0.3211076782166984 1106 28-29 0.2569557764446131 1106 30-31 0.3308053823079078 1106 32-33 0.4035173745138465 1106 34-35 0.20831209454668453 1106 36-37 0.1056142493385579 1106 38-39 0.20339954647477043 1106 40-41 0.27290160423552834 1106 42-43 0.19597211646142654 1106 44-45 0.21864116402260692 1106 46-47 0.33831504191530115 1106 48-49 0.30113908452270977 1106 50-51 -0.06273360710073206 1106 52-53 0.08146964897467512 1106 54-55 0.23279158488202967 1106 56-57 0.16667163077123348 1106 58-59 0.3068524390557812 1106 60-61 0.3078095403810863 1106 62-63 0.3878478091108235 1106 64-65 0.4457296810796194 1106 66-67 0.24748579146292826 1106 68-69 0.34837263770076987 1106 70-71 0.2079090227136362 1106 72-73 0.07308827779916172 1106 74-75 0.22980580462508726 1106 76-77 0.15600032253984963 1106 78-79 0.09905496187113272 1106 80-81 0.37970610505643876 1106 82-83 0.30250884081351614 1106 84-85 0.36962321430070944 1106 86-87 0.3980702188358727 1106 88-89 0.1829933941206967 1106 90-91 0.1644514472620422 1106 92-93 0.16382182131224354 1106 94-95 0.30920643893356115 1106 96-97 0.5057937933241305 1106 98-99 0.6327809568808966 1106 100-101 0.1870909510924612 1107 1 0.23484907097386198 1107 2 0.2828089957644977 1107 3 0.2588901034856761 1107 4 0.3386769459081833 1107 5 0.34160177945420855 1107 6 0.2940216492779868 1107 7 0.44289853355607534 1107 8 0.39692000856320675 1107 9 0.47419996372916984 1107 10-11 0.5652882338690404 1107 12-13 0.45504899121215914 1107 14-15 0.23598054970269544 1107 16-17 0.2739089885979382 1107 18-19 0.3659332550909582 1107 20-21 0.2694952780490141 1107 22-23 0.3361377494203097 1107 24-25 0.3803547053908787 1107 26-27 0.35791935008833065 1107 28-29 0.3763903054125137 1107 30-31 0.42632287802892677 1107 32-33 0.3195728969292091 1107 34-35 0.3577825425277368 1107 36-37 0.33351328125487356 1107 38-39 0.28747667346411276 1107 40-41 0.3614511516680139 1107 42-43 0.35425218622952315 1107 44-45 0.3278547772364391 1107 46-47 0.36590806751619454 1107 48-49 0.3960060044726532 1107 50-51 0.2938116884619859 1107 52-53 0.3451926153449918 1107 54-55 0.527862875297572 1107 56-57 0.4019195460400766 1107 58-59 0.4631181341297932 1107 60-61 0.56997146161941 1107 62-63 0.6397275849767539 1107 64-65 0.47255277302824794 1107 66-67 0.5583164868719024 1107 68-69 0.5893519465202743 1107 70-71 0.3749284464144438 1107 72-73 0.3231611153480358 1107 74-75 0.5269475599639399 1107 76-77 0.5385578385065131 1107 78-79 0.37195439607755887 1107 80-81 0.6281535664995026 1107 82-83 0.5178689152744056 1107 84-85 0.3864685367654772 1107 86-87 0.5020802005928147 1107 88-89 0.3156449254637046 1107 90-91 0.34175735823293607 1107 92-93 0.3476495284322816 1107 94-95 0.5237486416473764 1107 96-97 0.28407469957210907 1107 98-99 0.3314730178007359 1107 100-101 0.21901444366533696 1108 1 0.3334628882220301 1108 2 0.31295036444599944 1108 3 0.31358132722921184 1108 4 0.4890417327755614 1108 5 0.39135145208608435 1108 6 0.41959220252460483 1108 7 0.4422985116703302 1108 8 0.3080185406761302 1108 9 0.3952577942350999 1108 10-11 0.3772460017288637 1108 12-13 0.2859251663774316 1108 14-15 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-2.6082803562269667 2316 86-87 -2.60625533546051 2316 88-89 -2.9083684214838392 2316 90-91 -3.1430154142233917 2316 92-93 -3.913825072994836 2316 94-95 -3.728641096264095 2316 96-97 -4.030359466877357 2316 98-99 -5.272167909425967 2316 100-101 -5.786746172438349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5455.0 3 2275.0 4 2389.0 5 2535.0 6 2886.0 7 3343.0 8 4218.0 9 5228.0 10 6651.0 11 8114.0 12 9974.0 13 11477.0 14 13424.0 15 15358.0 16 17820.0 17 21654.0 18 18371.0 19 15264.0 20 15674.0 21 16863.0 22 18766.0 23 21168.0 24 24453.0 25 27432.0 26 32228.0 27 37564.0 28 44767.0 29 52906.0 30 64561.0 31 78412.0 32 97314.0 33 124789.0 34 164212.0 35 222408.0 36 308691.0 37 386993.0 38 276705.0 39 37016.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.586590887275904 17.84817353913098 14.24527268819007 23.319962885403047 2 15.807643760440648 19.09051949649654 40.254680858063054 24.847155884999758 3 19.996917588761278 23.035616270444635 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26.133126605765845 27.672048432285905 22.885998848309367 48-49 23.27329025467355 26.162603391712263 27.50594655825059 23.0581597953636 50-51 23.073640668862563 26.401123782831448 27.612231839912766 22.913003708393227 52-53 23.384920123534336 26.38397117652307 27.221283989290328 23.009824710652268 54-55 23.048193799866038 26.23687145954381 27.418054273533254 23.2968804670569 56-57 23.14004435524932 26.440058683743107 27.153982645400422 23.26591431560715 58-59 23.388956416631277 26.16572472037319 27.20652562067633 23.238793242319204 60-61 23.143220447048254 26.217527676357506 27.678047215512187 22.961204661082053 62-63 22.903899716741837 26.346568944514697 27.672292854653392 23.077238484090074 64-65 22.89004623189029 26.446416538495072 27.417036162074982 23.24650106753965 66-67 22.863489318094402 26.44355510870954 27.20049193119723 23.492463641998828 68-69 22.902016378497223 26.344656140584167 27.430996537008255 23.32233094391035 70-71 23.133925494289553 26.203967570070137 27.211604413279872 23.450502522360438 72-73 23.068470075351826 26.26451235817737 27.343191274491822 23.323826291978982 74-75 22.933411516655795 26.751226046404298 26.998440990896995 23.316921446042915 76-77 23.08913325697175 26.430331848391237 27.054151749830258 23.426383144806756 78-79 23.03677407531717 26.494451869201747 27.164460766137278 23.304313289343806 80-81 23.199641966568514 26.503965874093076 26.91800403125803 23.378388128080374 82-83 23.14869619508501 26.529843597505316 27.080576924377286 23.240883283032392 84-85 23.130142442053696 26.578382562199398 26.96365096908592 23.327824026660988 86-87 23.10412256701757 26.67133257290779 26.894097298115803 23.330447561958835 88-89 23.10668139167061 26.58975623748959 26.628040947288557 23.67552142355125 90-91 22.98144793004587 26.508317746878067 26.93805992636146 23.572174396714594 92-93 23.16492880913493 26.388348061856192 27.15584922463431 23.29087390437457 94-95 22.925671802673513 26.3091319739859 27.260168969097435 23.505027254243156 96-97 22.846607779289734 26.230187137657524 27.30396805921996 23.61923702383278 98-99 22.701585455463444 26.441763700868687 26.981188212542094 23.875462631125778 100-101 22.85576637372755 26.183828877024634 27.010884782541062 23.94951996670675 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 73.0 2 102.0 3 145.0 4 133.5 5 147.5 6 261.5 7 530.0 8 947.0 9 1254.5 10 1225.0 11 1151.0 12 1257.0 13 1408.0 14 1572.0 15 1744.5 16 1896.0 17 2089.0 18 2357.0 19 2633.5 20 2985.0 21 3546.5 22 4326.0 23 5219.5 24 6524.5 25 8200.5 26 10478.5 27 13458.5 28 16287.5 29 19368.5 30 23116.5 31 27235.5 32 32358.5 33 35852.5 34 38106.5 35 41643.0 36 46100.0 37 49938.5 38 52601.0 39 55169.0 40 57487.5 41 60301.5 42 62945.5 43 65946.0 44 69840.0 45 74400.0 46 77783.5 47 82698.0 48 91528.0 49 92624.5 50 85698.5 51 82240.5 52 82180.5 53 82412.0 54 82424.5 55 77969.5 56 71276.5 57 65352.0 58 61206.5 59 55728.0 60 47047.0 61 39494.5 62 32684.5 63 25800.5 64 18906.5 65 13859.0 66 10500.0 67 8202.0 68 6407.5 69 4993.0 70 3944.5 71 2944.5 72 2284.5 73 1915.5 74 1454.5 75 1085.5 76 803.0 77 488.5 78 314.0 79 213.5 80 153.5 81 110.5 82 69.0 83 44.0 84 30.5 85 24.5 86 17.0 87 12.5 88 12.0 89 7.0 90 3.0 91 3.0 92 1.0 93 0.5 94 2.0 95 4.0 96 3.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013292072337710556 2 0.012481030635748555 3 0.014688866379978445 4 0.017617628081507887 5 0.029422790632288108 6 0.045102930203553444 7 0.005136597445759334 8 0.023880672335547776 9 0.034153867227066446 10-11 0.03859206765169183 12-13 0.0302113033980845 14-15 0.024939532335331503 16-17 0.027327599568886276 18-19 0.023632854037726053 20-21 0.05064504850029378 22-23 0.012661262125073444 24-25 0.002838645956867 26-27 9.912731912868889E-4 28-29 0.0 30-31 0.003063935318523111 32-33 0.0021177199995674446 34-35 0.0 36-37 2.9287617015294443E-4 38-39 4.5057872331222216E-5 40-41 0.0015544965954271668 42-43 0.003897505956650722 44-45 0.0019149595740769446 46-47 9.912731912868889E-4 48-49 7.659838296307777E-4 50-51 0.003514514041835333 52-53 0.002973819573860667 54-55 0.0049338370202688326 56-57 6.30810212637111E-4 58-59 0.005204184254256166 60-61 0.0024781829782172223 62-63 6.533391488027223E-4 64-65 5.632234041402778E-4 66-67 0.016153247230743167 68-69 0.041025192757577836 70-71 0.04994665147915983 72-73 0.01401299829501011 74-75 0.029332674887625663 76-77 0.024263664250363165 78-79 0.053551281265657606 80-81 0.07650826721841533 82-83 0.047445939564777 84-85 0.01782038850699839 86-87 0.019600174464081667 88-89 0.021019497442515165 90-91 0.03733044722641761 92-93 0.024511482548184887 94-95 0.011895278295442667 96-97 0.017414867656017387 98-99 0.04857238637305755 100-101 0.06763186636916456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2219368.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.53747700919793 #Duplication Level Percentage of deduplicated Percentage of total 1 79.38627419896764 47.26458474966918 2 12.12564756580254 14.438609263412111 3 3.7395308059329313 6.679266881502579 4 1.589147313466282 3.784552865589096 5 0.8249405183272049 2.45574385719309 6 0.4964002703426428 1.7732651809730837 7 0.32367833530989204 1.3489694012821707 8 0.2293848437146675 1.0925595887136381 9 0.17444256297253408 0.9347283074162528 >10 0.9957353385414742 10.988416351055577 >50 0.0755151940149247 3.0649350835176 >100 0.03574016155709376 3.7627718331007176 >500 0.002729022932072152 1.0952899364888293 >1k 7.580619255754295E-4 1.0423036835861519 >5k 7.580619255754295E-5 0.2740030165000229 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6071 0.27354634292285007 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4860 0.21898125952974 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3907 0.17604110719808522 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2833 0.12764895231435255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2763 0.12449490125116701 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 4.505787233122222E-5 3 0.0 0.0 0.0 0.0 4.505787233122222E-5 4 0.0 0.0 0.0 0.0 4.505787233122222E-5 5 0.0 0.0 0.0 0.0 4.505787233122222E-5 6 0.0 0.0 0.0 0.0 4.505787233122222E-5 7 0.0 0.0 0.0 0.0 4.505787233122222E-5 8 0.0 0.0 0.0 0.0 4.505787233122222E-5 9 0.0 0.0 0.0 0.0 4.505787233122222E-5 10-11 2.252893616561111E-5 0.0 0.0 0.0 6.758680849683334E-5 12-13 4.505787233122222E-5 0.0 0.0 0.0 3.1540510631855557E-4 14-15 4.505787233122222E-5 0.0 0.0 0.0 4.505787233122222E-4 16-17 4.505787233122222E-5 0.0 0.0 0.0 4.956365956434444E-4 18-19 1.1264468082805555E-4 0.0 0.0 0.0 5.406944679746667E-4 20-21 1.802314893248889E-4 0.0 0.0 0.0 6.308102126371111E-4 22-23 1.802314893248889E-4 0.0 0.0 1.802314893248889E-4 6.758680849683333E-4 24-25 1.802314893248889E-4 0.0 0.0 3.1540510631855557E-4 6.758680849683333E-4 26-27 1.802314893248889E-4 0.0 0.0 5.857523403058889E-4 7.209259572995556E-4 28-29 2.7034723398733335E-4 0.0 0.0 0.0013967940422678889 7.209259572995556E-4 30-31 3.1540510631855557E-4 0.0 0.0 0.0030414063823575 7.659838296307777E-4 32-33 3.1540510631855557E-4 0.0 0.0 0.0054745314882435 9.011574466244444E-4 34-35 3.8299191481538887E-4 0.0 0.0 0.008178003828116833 9.462153189556667E-4 36-37 4.505787233122222E-4 0.0 0.0 0.009642384678881556 9.462153189556667E-4 38-39 4.956365956434444E-4 0.0 0.0 0.011129294465811889 0.0011940336167773889 40-41 5.181655318090556E-4 0.0 0.0 0.012841493614398333 0.0012616204252742223 42-43 5.857523403058889E-4 0.0 0.0 0.01543232127344361 0.0012616204252742223 44-45 6.308102126371111E-4 0.0 0.0 0.019127066804603833 0.0013066782976054443 46-47 7.209259572995555E-4 0.0 0.0 0.023092159569751386 0.0013066782976054443 48-49 7.659838296307777E-4 0.0 0.0 0.027800707228364113 0.0013066782976054443 50-51 7.659838296307777E-4 0.0 0.0 0.03352305701442933 0.0013517361699366666 52-53 7.659838296307777E-4 0.0 0.0 0.04649972424582133 0.0014418519145991111 54-55 7.659838296307777E-4 2.252893616561111E-5 0.0 0.06332883956153283 0.0015319676592615555 56-57 9.912731912868889E-4 4.505787233122222E-5 0.0 0.0837400557275765 0.0015770255315927777 58-59 9.912731912868889E-4 4.505787233122222E-5 0.0 0.11235180465790261 0.0015770255315927777 60-61 9.912731912868889E-4 4.505787233122222E-5 0.0 0.151664798266894 0.0015770255315927777 62-63 9.912731912868889E-4 4.505787233122222E-5 0.0 0.17786595102749972 0.0017797859570832777 64-65 9.912731912868889E-4 4.505787233122222E-5 0.0 0.20778437825543128 0.0018023148932488889 66-67 0.0010363310636181111 4.505787233122222E-5 0.0 0.268454803349422 0.0018023148932488889 68-69 0.0010363310636181111 4.505787233122222E-5 0.0 0.3443998471636971 0.0018023148932488889 70-71 0.0010813889359493332 4.505787233122222E-5 0.0 0.3986945833228198 0.0018023148932488889 72-73 0.0011264468082805555 4.505787233122222E-5 0.0 0.4472669696958774 0.0019600174464081666 74-75 0.0011264468082805555 4.505787233122222E-5 0.0 0.5522743411637907 0.002027604254905 76-77 0.0011264468082805555 4.505787233122222E-5 0.0 0.6292106581693527 0.002027604254905 78-79 0.0011264468082805555 4.505787233122222E-5 0.0 0.69030913305049 0.0020726621272362223 80-81 0.0012616204252742223 4.505787233122222E-5 0.0 0.7751981645225128 0.0020951910634018334 82-83 0.0012616204252742223 4.505787233122222E-5 0.0 0.9172205781105252 0.002275422552726722 84-85 0.0012841493614398334 4.505787233122222E-5 0.0 1.0695161865900564 0.002297951488892333 86-87 0.0013066782976054443 4.505787233122222E-5 0.0 1.2691450899535364 0.0023430093612235555 88-89 0.0013066782976054443 4.505787233122222E-5 0.0 1.5351667681970724 0.0023880672335547777 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8365 0.0 24.702635 1 GTATCAA 13355 0.0 24.258307 1 TACATGG 8435 0.0 23.822374 2 ACGACGG 160 1.8573228E-6 23.75519 6 ACATGGG 8380 0.0 22.846527 3 GAGTACT 7065 0.0 21.450027 12-13 TCAACGC 15025 0.0 21.342655 4 ATCAACG 15125 0.0 21.201546 3 CAACGCA 15245 0.0 21.066296 5 GGTATCA 9965 0.0 20.831673 1 TATCAAC 15395 0.0 20.766586 2 GTGTAGC 1490 0.0 20.722734 1 AACGCAG 15505 0.0 20.652689 6 TAGATCG 140 4.848021E-4 20.360674 5 AGTACTT 7630 0.0 20.235231 12-13 GTACTTT 7500 0.0 20.175848 14-15 GTATAGG 615 0.0 20.082521 1 TAAGGTG 890 0.0 19.750616 5 TAGACCG 145 6.1413314E-4 19.658583 5 CATGGGG 4785 0.0 19.16172 4 >>END_MODULE