FastQCFastQC Report
Thu 26 May 2016
SRR936659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219368
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64940.2926058229189571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60490.2725550697315632No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50830.22902916505960258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33590.15134939316057544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1703.1548589E-622.3499364
TACATGG84450.021.9928152
GTACATG86250.021.9208321
GTATCAA149650.021.7443731
GAGTACT85300.021.63346712-13
ACATGGG82800.021.397483
GTACTTT88400.020.92997414-15
GGTATCA117750.020.8978631
GTAAGGT8400.019.7890074
AGAGTAC131800.019.08204510-11
AGTACTT89800.019.041912-13
TAAGGTG10400.018.7234425
TCAACGC174750.018.6713094
ATCAACG174950.018.6520653
CAACGCA177000.018.4339625
TATCAAC176850.018.4256482
AACGCAG179850.018.1418466
ACTTTTT102850.018.0110716-17
GTATACG1350.00939816117.594191
GTACGGT1350.00941036317.5902276
Produced by FastQC (version 0.11.5)