##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936659_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219368 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96207343712264 31.0 31.0 34.0 30.0 34.0 2 32.08630745329301 33.0 31.0 34.0 30.0 34.0 3 32.20865309403398 34.0 31.0 34.0 30.0 34.0 4 35.92521204234719 37.0 35.0 37.0 35.0 37.0 5 35.79314831970183 37.0 35.0 37.0 35.0 37.0 6 35.841649965215325 37.0 35.0 37.0 35.0 37.0 7 35.76969885120449 37.0 35.0 37.0 33.0 37.0 8 35.73576261350078 37.0 35.0 37.0 33.0 37.0 9 37.35847051953529 39.0 37.0 39.0 34.0 39.0 10-11 37.26361153265254 39.0 37.0 39.0 33.5 39.0 12-13 37.139039357150324 39.0 37.0 39.0 33.0 39.0 14-15 38.259008420415185 40.0 38.0 41.0 33.0 41.0 16-17 38.23411394595218 40.0 37.5 41.0 33.0 41.0 18-19 38.36665302915064 40.0 38.0 41.0 33.5 41.0 20-21 38.3685328435843 40.0 38.0 41.0 34.0 41.0 22-23 38.26586127221804 40.0 38.0 41.0 34.0 41.0 24-25 38.15209532623702 40.0 37.5 41.0 33.0 41.0 26-27 37.949804629065575 40.0 37.0 41.0 33.0 41.0 28-29 37.979734996629674 40.0 37.0 41.0 33.0 41.0 30-31 37.864513005504264 40.0 37.0 41.0 33.0 41.0 32-33 37.67858575053799 40.0 37.0 41.0 33.0 41.0 34-35 37.510002171789445 40.0 36.5 41.0 32.0 41.0 36-37 37.339328133054096 39.5 36.0 41.0 32.0 41.0 38-39 37.12976960107562 39.0 36.0 41.0 31.0 41.0 40-41 36.90825744085704 39.0 36.0 41.0 31.0 41.0 42-43 36.64609924987654 39.0 35.0 40.5 30.5 41.0 44-45 36.37112299537526 39.0 35.0 40.0 30.0 41.0 46-47 36.02128669062544 39.0 35.0 40.0 29.5 41.0 48-49 35.63290270022817 38.0 35.0 40.0 28.0 41.0 50-51 35.32593242761003 38.0 34.5 40.0 27.0 41.0 52-53 35.0516279859852 38.0 34.0 40.0 27.0 41.0 54-55 34.712818469041636 38.0 34.0 40.0 26.0 41.0 56-57 34.353582641544804 37.5 33.0 40.0 25.5 41.0 58-59 33.96921601104459 37.0 33.0 40.0 24.5 41.0 60-61 33.58238246203423 36.5 32.5 39.0 23.5 40.5 62-63 33.234838251249904 36.0 32.0 39.0 23.0 40.0 64-65 32.90039033634801 36.0 32.0 39.0 22.0 40.0 66-67 32.44060764145469 35.0 31.0 38.5 21.5 40.0 68-69 31.97611775063892 35.0 31.0 38.0 20.0 40.0 70-71 31.46761938533853 35.0 30.5 37.0 18.5 39.0 72-73 30.898875941258954 34.0 30.0 36.5 17.5 39.0 74-75 30.366369164554953 34.0 29.0 36.0 14.0 39.0 76-77 29.002249288986775 32.5 28.0 34.5 12.5 36.5 78-79 29.787166887149855 34.0 29.0 35.0 11.5 37.0 80-81 29.645686970344713 34.0 29.0 35.0 7.5 37.0 82-83 29.236794438777167 34.0 29.0 35.0 7.0 36.0 84-85 28.78802186027734 33.0 29.0 35.0 2.0 36.0 86-87 28.361956421828197 33.0 27.5 35.0 2.0 35.5 88-89 27.860915584977345 33.0 27.0 35.0 2.0 35.0 90-91 27.273174840765478 32.5 26.0 34.0 2.0 35.0 92-93 26.68435924100915 32.0 25.0 34.0 2.0 35.0 94-95 25.91999096139081 32.0 24.0 34.0 2.0 35.0 96-97 25.067112123811825 31.0 21.0 34.0 2.0 35.0 98-99 23.89126431488604 31.0 8.5 34.0 2.0 35.0 100-101 20.05057408235137 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2775744168277363 1101 2 0.4127060861659828 1101 3 0.29932637183537736 1101 4 0.22598973124723187 1101 5 0.19134787391570995 1101 6 0.20112266453782013 1101 7 0.2319136916310498 1101 8 0.22486262956876146 1101 9 0.27507496207856263 1101 10-11 0.31715683096843605 1101 12-13 0.363866557724549 1101 14-15 0.4234562004064486 1101 16-17 0.3583859953024984 1101 18-19 0.378342839811161 1101 20-21 0.2708234483673593 1101 22-23 0.26497327777443047 1101 24-25 0.3105409578718792 1101 26-27 0.2364743556069655 1101 28-29 0.34492522710624485 1101 30-31 0.40067401573349315 1101 32-33 0.32127647757149447 1101 34-35 0.4967803710309582 1101 36-37 0.5248804766369446 1101 38-39 0.42260948322679326 1101 40-41 0.4112450789692659 1101 42-43 0.4368917746252734 1101 44-45 0.60709558457323 1101 46-47 0.6101579816236864 1101 48-49 0.7337151729398883 1101 50-51 0.7675693773268932 1101 52-53 0.6262821903456128 1101 54-55 0.6034010609379123 1101 56-57 0.6330904334636855 1101 58-59 0.606157947841794 1101 60-61 0.7350522050189028 1101 62-63 0.6174649199562054 1101 64-65 0.6173157050641365 1101 66-67 0.4615718012217016 1101 68-69 0.42893744129201394 1101 70-71 0.501015759743467 1101 72-73 0.49481755117611925 1101 74-75 0.5222445648309844 1101 76-77 0.4886875706268903 1101 78-79 0.5888540519905163 1101 80-81 0.7318068841721939 1101 82-83 0.5932856397357291 1101 84-85 0.5780718905038356 1101 86-87 0.5626846551384794 1101 88-89 0.44077089320723317 1101 90-91 0.44268849481445827 1101 92-93 0.47116465565640553 1101 94-95 0.4757253586345449 1101 96-97 0.6240698654402763 1101 98-99 0.32130435631671617 1101 100-101 0.3201451604150769 1102 1 0.2195905847483779 1102 2 0.3030949683866737 1102 3 0.31089829044406514 1102 4 0.25302470699254087 1102 5 0.11148996968084646 1102 6 0.12685692074959576 1102 7 0.19071364081237618 1102 8 0.08257088963966908 1102 9 0.28416574992952803 1102 10-11 0.26070394669253716 1102 12-13 0.2749242530693792 1102 14-15 0.3692806808310749 1102 16-17 0.4000243221325803 1102 18-19 0.34315406315855057 1102 20-21 0.30986756585958375 1102 22-23 0.30672454280156813 1102 24-25 0.23346235808091365 1102 26-27 0.3095471940758543 1102 28-29 0.43185822857395095 1102 30-31 0.5121273808214752 1102 32-33 0.41977936209305966 1102 34-35 0.3076241927360144 1102 36-37 0.3673229178146826 1102 38-39 0.46656202531891466 1102 40-41 0.6185933616980037 1102 42-43 0.5668323995967555 1102 44-45 0.68769323750832 1102 46-47 0.5289217260057342 1102 48-49 0.72571200308969 1102 50-51 0.6603649872668598 1102 52-53 0.6330407237073032 1102 54-55 0.6138309630979109 1102 56-57 0.7730061895705234 1102 58-59 0.7419712888681218 1102 60-61 0.5992448302739604 1102 62-63 0.4365996968488659 1102 64-65 0.5525535456071964 1102 66-67 0.554762168414733 1102 68-69 0.3235050368034109 1102 70-71 0.0700784850144558 1102 72-73 0.23083374576954796 1102 74-75 0.5135081039027583 1102 76-77 0.6128434295503062 1102 78-79 0.5167935861853792 1102 80-81 0.6755892117659634 1102 82-83 0.6177619230653058 1102 84-85 0.37280352365754865 1102 86-87 0.5066540432015643 1102 88-89 0.26013975890483465 1102 90-91 0.17782961140752818 1102 92-93 0.36548166734961285 1102 94-95 0.4033868641168574 1102 96-97 0.401391608745584 1102 98-99 0.13394360756946 1102 100-101 0.10671042837481437 1103 1 0.21077135638254063 1103 2 0.22346637665016544 1103 3 0.17028281266607337 1103 4 0.1317350854226902 1103 5 0.1491968414638265 1103 6 0.127208229675972 1103 7 0.1677870828453294 1103 8 0.17837300565321357 1103 9 0.12031406414057955 1103 10-11 0.18678970815893337 1103 12-13 0.2052987320649251 1103 14-15 0.24262500867359904 1103 16-17 0.2456448784289762 1103 18-19 0.2591029730486767 1103 20-21 0.18658647791854577 1103 22-23 0.2593287597970999 1103 24-25 0.2593997366205798 1103 26-27 0.15869887750270806 1103 28-29 0.24930664349641773 1103 30-31 0.3040683196924405 1103 32-33 0.26487896762210283 1103 34-35 0.15192369003784734 1103 36-37 0.17937908429559712 1103 38-39 0.06411037861200697 1103 40-41 0.3277692674029069 1103 42-43 0.4056380780889626 1103 44-45 0.35422204333713125 1103 46-47 0.31587175896778774 1103 48-49 0.39208899020763965 1103 50-51 0.4680819731393484 1103 52-53 0.4293592810215472 1103 54-55 0.28176822567986193 1103 56-57 0.2889894573018523 1103 58-59 0.26772592663984085 1103 60-61 0.14715018301089344 1103 62-63 0.3367154398264489 1103 64-65 0.3638669385084441 1103 66-67 0.27052361333374364 1103 68-69 0.28893095592934515 1103 70-71 0.12148597545331441 1103 72-73 0.4706061680188256 1103 74-75 0.34824559925908005 1103 76-77 0.22049728288956416 1103 78-79 0.03715361341397028 1103 80-81 0.21796896469014015 1103 82-83 0.06487704545438078 1103 84-85 0.2594348876423709 1103 86-87 0.3452552925385639 1103 88-89 0.13760080033394928 1103 90-91 0.08996523039624904 1103 92-93 0.33753006036895883 1103 94-95 0.3939659440568164 1103 96-97 0.3642696792092224 1103 98-99 0.4770704288034615 1103 100-101 0.4306571994893176 1104 1 0.11773652252191269 1104 2 0.20687073030280345 1104 3 0.12884343778763707 1104 4 0.07346050445149643 1104 5 -0.061249480201119866 1104 6 0.059393192997013955 1104 7 0.16002347344280565 1104 8 0.08945797486287432 1104 9 0.09734577167961334 1104 10-11 0.06807112528196768 1104 12-13 0.15146002135139014 1104 14-15 0.1442647661338583 1104 16-17 0.06849133892258408 1104 18-19 0.12229785244796432 1104 20-21 0.15886825549646488 1104 22-23 0.016376143554857947 1104 24-25 -0.015145769024471178 1104 26-27 -0.003764787038697648 1104 28-29 0.025355085421246315 1104 30-31 0.18053935928359266 1104 32-33 0.26370458954061604 1104 34-35 0.12306410149174951 1104 36-37 0.0027470444956705364 1104 38-39 0.24054680025856356 1104 40-41 0.02228955012802203 1104 42-43 0.05945394809679527 1104 44-45 0.2963117091668934 1104 46-47 0.12278727333870165 1104 48-49 -0.019038136163921138 1104 50-51 0.0055289086482588345 1104 52-53 0.055124355441208195 1104 54-55 -0.10145576669705747 1104 56-57 0.05194375090842129 1104 58-59 0.16314181484886348 1104 60-61 -0.03295458088223313 1104 62-63 -0.020865622373435144 1104 64-65 -0.05894084446203607 1104 66-67 0.036010013299481614 1104 68-69 0.05647034276346119 1104 70-71 -0.02204042429828945 1104 72-73 0.1150894684294208 1104 74-75 0.1319751206927613 1104 76-77 -0.005807269370148305 1104 78-79 -0.02072995430426161 1104 80-81 -0.07074112429939206 1104 82-83 -0.10175214817948586 1104 84-85 -0.08364723361679438 1104 86-87 -0.08668631912304292 1104 88-89 -0.04596352748014354 1104 90-91 0.083143130942819 1104 92-93 0.0901812774395303 1104 94-95 0.0556779174045694 1104 96-97 0.5109848556090597 1104 98-99 0.364225297710302 1104 100-101 0.13023500331977544 1105 1 0.15642656171456437 1105 2 0.21207727922836028 1105 3 0.13719054439592782 1105 4 0.08264592975390883 1105 5 0.12202010075247927 1105 6 0.11353847814459073 1105 7 0.0905318108234141 1105 8 0.18996052684051534 1105 9 0.25930442550043153 1105 10-11 0.22991494952116653 1105 12-13 0.1321775932892919 1105 14-15 0.24647423160529058 1105 16-17 0.27276485043628895 1105 18-19 0.17505420164535224 1105 20-21 0.1428540781217862 1105 22-23 0.07365806460983748 1105 24-25 0.14731126400504024 1105 26-27 0.16728093395929733 1105 28-29 0.15930896910829517 1105 30-31 0.19743970101139752 1105 32-33 0.2530306151946675 1105 34-35 0.04838816563066217 1105 36-37 0.18120429337968602 1105 38-39 0.12436750045547029 1105 40-41 0.14864099390607777 1105 42-43 0.29377767088437423 1105 44-45 0.18720211531222475 1105 46-47 0.3840231596536512 1105 48-49 0.30606809688617176 1105 50-51 0.4932162596992242 1105 52-53 0.4345205087129784 1105 54-55 0.3371287610521705 1105 56-57 0.4439225281427994 1105 58-59 0.43131626294526626 1105 60-61 0.4138962649035136 1105 62-63 0.48431300351178663 1105 64-65 0.2534448750049094 1105 66-67 0.49619003219930136 1105 68-69 0.21959282263063074 1105 70-71 0.16719072576702843 1105 72-73 0.1372915797877603 1105 74-75 0.37977531847573687 1105 76-77 0.2162951033980498 1105 78-79 0.33818700194629514 1105 80-81 0.5073718554592297 1105 82-83 0.28745989499643443 1105 84-85 0.3955174721622079 1105 86-87 0.4572261129674935 1105 88-89 0.49239049400240376 1105 90-91 0.5375251971976063 1105 92-93 0.519141297920612 1105 94-95 0.6994538089652842 1105 96-97 0.7700010170883189 1105 98-99 0.7212687434511302 1105 100-101 0.5338010579384971 1106 1 0.06224667077359314 1106 2 0.18526313733191557 1106 3 0.15525935483359632 1106 4 0.07791940373353157 1106 5 0.11803530399173923 1106 6 0.08813263558980822 1106 7 0.08667114573611201 1106 8 0.14907223378365586 1106 9 0.16434573584117373 1106 10-11 0.08826608639725464 1106 12-13 0.17262411362342078 1106 14-15 0.1878039913067724 1106 16-17 0.22224870073553404 1106 18-19 0.11434769177228787 1106 20-21 0.09957803580682167 1106 22-23 0.05125739885595948 1106 24-25 0.125393181387075 1106 26-27 0.07630874149467104 1106 28-29 0.24374707433522502 1106 30-31 0.24808287312320232 1106 32-33 0.13552094142631432 1106 34-35 0.1550767882996169 1106 36-37 0.1660643102615964 1106 38-39 0.16033439776899172 1106 40-41 -0.0930086854261063 1106 42-43 0.18660425451545848 1106 44-45 0.18976551939041286 1106 46-47 0.14692894269325052 1106 48-49 0.2200953500800935 1106 50-51 0.18547819725521464 1106 52-53 0.27491404352731763 1106 54-55 0.20678319207679863 1106 56-57 0.30667591829783447 1106 58-59 0.35776246495816366 1106 60-61 0.35912392919433955 1106 62-63 0.4033749013456429 1106 64-65 0.3948186079266023 1106 66-67 0.14602954524945488 1106 68-69 0.16611655762685018 1106 70-71 0.1418054584622226 1106 72-73 0.31627601945738704 1106 74-75 0.3130861720731417 1106 76-77 0.28828297182255724 1106 78-79 0.1568569837788587 1106 80-81 0.09337504003180541 1106 82-83 0.12317587628621851 1106 84-85 0.16285254537949 1106 86-87 0.26503661389491384 1106 88-89 0.12816350784461505 1106 90-91 0.09744117535859331 1106 92-93 0.002036633494437723 1106 94-95 -0.031402026209619294 1106 96-97 0.07181612823207928 1106 98-99 0.09717515973230562 1106 100-101 -0.0061451947473365465 1107 1 0.07309928833534585 1107 2 0.14252699037799488 1107 3 0.05742736181749564 1107 4 0.022115030857740692 1107 5 0.06907766071000054 1107 6 0.17084224202956477 1107 7 0.026732222944971795 1107 8 -0.042368060515485695 1107 9 0.09433130656324806 1107 10-11 0.11646883848695211 1107 12-13 0.18118899415743783 1107 14-15 0.18803083330812598 1107 16-17 0.21465472168935662 1107 18-19 0.22807422027091206 1107 20-21 0.14451782874970576 1107 22-23 0.13359883009430717 1107 24-25 0.19414987809967243 1107 26-27 0.2371822533313903 1107 28-29 0.2319030863394218 1107 30-31 0.2780472837398307 1107 32-33 0.1643947382332982 1107 34-35 0.09295672649934517 1107 36-37 0.1959233634018176 1107 38-39 0.15628597692603563 1107 40-41 0.08653029010579871 1107 42-43 0.128265188104983 1107 44-45 0.11997784350368335 1107 46-47 0.3045515250980344 1107 48-49 0.30572519284668687 1107 50-51 0.23757086788381798 1107 52-53 0.2258095732157983 1107 54-55 0.2555785901614698 1107 56-57 0.26398017757539805 1107 58-59 0.37467149812565737 1107 60-61 0.12144871590082573 1107 62-63 -0.004903573439626996 1107 64-65 -0.054265464326825 1107 66-67 -0.02195271641492269 1107 68-69 -0.018180340273332263 1107 70-71 -0.010045589990873793 1107 72-73 0.03236260859371143 1107 74-75 -0.07322194599954202 1107 76-77 -0.3219983051703146 1107 78-79 -0.15863797306267813 1107 80-81 0.027837018654071954 1107 82-83 0.0817417669760232 1107 84-85 0.1909102426425342 1107 86-87 0.35550643203497145 1107 88-89 0.26075265763724786 1107 90-91 0.256325034734985 1107 92-93 0.13817532894735152 1107 94-95 0.3566873332346461 1107 96-97 0.45771974093905854 1107 98-99 0.691783603854919 1107 100-101 0.3785734343330276 1108 1 0.01087125357960872 1108 2 0.1801532097921168 1108 3 -0.13060330319329694 1108 4 0.0365845916813754 1108 5 0.011705433424353373 1108 6 0.019656923397384674 1108 7 0.05589754623338905 1108 8 0.03332484725520146 1108 9 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-1.2561483952613983 2316 100-101 -0.9902880150232853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 22.0 5 69.0 6 160.0 7 345.0 8 852.0 9 1600.0 10 2610.0 11 3954.0 12 5749.0 13 8155.0 14 10538.0 15 13720.0 16 17187.0 17 21661.0 18 19904.0 19 15104.0 20 14450.0 21 14844.0 22 16140.0 23 18118.0 24 20932.0 25 25209.0 26 30672.0 27 37899.0 28 46819.0 29 57514.0 30 71254.0 31 87313.0 32 109647.0 33 139994.0 34 185113.0 35 250886.0 36 349057.0 37 414812.0 38 199698.0 39 7357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23791361213605 18.947568121966125 14.797522247677897 23.016996018219935 2 16.07819033332147 20.152094338865933 39.077321982435606 24.69239334537699 3 20.15359229145676 23.356293021252206 29.24332162368221 27.24679306360882 4 13.517822037804608 16.451687176342357 35.99246449087327 34.038026294979765 5 14.178354929978564 37.150946108345664 33.76709868969187 14.903600271983903 6 33.329037816171095 35.58846482422023 16.448060889406353 14.634436470202328 7 29.65479206148805 32.14492659588944 19.611166832930966 18.589114509691544 8 27.014135366261932 34.564352166219756 18.807638531861233 19.613873935657068 9 26.2423711286787 16.758712514615667 19.633588886911348 37.36532746979429 10-11 25.685420353902554 26.175312070823765 26.377802149080274 21.761465426193404 12-13 26.629112432007673 23.28054653396823 27.090076994892236 23.00026403913186 14-15 23.20601149120497 25.052949073591158 25.489653612466174 26.2513858227377 16-17 22.799414314156603 28.07919330539182 26.188308497559003 22.93308388289257 18-19 22.63978189750165 26.706246295323776 28.499450395582258 22.154521411592317 20-21 23.453048911678266 25.66015164298677 28.647547770019028 22.23925167531594 22-23 22.956296057656132 25.591374109260983 28.66542907543374 22.786900757649146 24-25 23.031802643961015 25.718298455027227 28.85140960714116 22.398489293870597 26-27 22.730072462135585 25.84783221875076 28.856056189019746 22.56603913009391 28-29 22.48374818501217 26.36685902627663 28.67316927007357 22.47622351863763 30-31 22.805273323226814 25.875055393233946 28.504057139007898 22.815614144531338 32-33 22.67881623903221 25.862367679644212 28.621750498190295 22.83706558313329 34-35 23.244851700915063 25.931517320728435 28.364076951376973 22.45955402697953 36-37 23.24915003017784 25.74984449230144 28.531297656266368 22.469707821254346 38-39 22.84820739993741 26.05652318791699 28.531122134362153 22.564147277783448 40-41 23.09647300333834 25.95048498062736 28.376987672611154 22.576054343423145 42-43 23.024365670473294 26.072197866790063 27.928770442811267 22.97466601992537 44-45 23.058593880252808 26.017419461239466 28.005868670473973 22.91811798803375 46-47 23.493174633499265 26.47670417884731 27.468157601623528 22.561963586029897 48-49 23.56062427187769 26.446663991674935 27.278292036552614 22.714419699894766 50-51 23.322139259208527 26.728551737935202 27.291266864894748 22.658042137961516 52-53 23.61105322385696 26.75005435143809 26.953018954852826 22.68587346985213 54-55 23.29938290865827 26.653475196830083 27.08064722160205 22.966494672909597 56-57 23.337754032479747 26.823842574442143 26.886593069461306 22.951810323616808 58-59 23.639954418449985 26.508446960531586 26.896944513159216 22.954654107859213 60-61 23.3368029036248 26.704944588258883 27.346071612832002 22.612180895284315 62-63 23.10526279031678 26.69020794015584 27.369907016175127 22.834622253352254 64-65 23.113324127701002 26.83887912977711 27.137735500058913 22.910061242462973 66-67 23.097102276324257 26.776385200051095 26.95107634210176 23.175436181522883 68-69 23.16012775994072 26.67994701977206 27.040508155994736 23.119417064292485 70-71 23.347978615379866 26.626415985339246 26.91441758587384 23.111187813407046 72-73 23.30897654868124 26.689863175294725 26.9409333891167 23.060226886907333 74-75 23.191038941024747 27.15726193469544 26.51351702098322 23.13818210329659 76-77 23.34481420852928 26.86522542932751 26.654014273593578 23.13594608854963 78-79 23.287974931664994 26.902295136926856 26.68735417134174 23.1223757600664 80-81 23.505285310331605 26.90214960811044 26.50299428448868 23.08957079706927 82-83 23.45423786169017 26.981566815085305 26.569632433501685 22.994562889722843 84-85 23.340062906982062 27.172788280673917 26.455988430630534 23.03116038171349 86-87 23.2751863891588 27.133323135145293 26.327208187447802 23.264282288248104 88-89 23.250221408257904 27.1693559706333 26.103644973576323 23.476777647532472 90-91 23.23777615805856 27.07561697971433 26.335635612743914 23.350971249483198 92-93 23.305863735534864 26.99537962836964 26.491797871583046 23.206958764512446 94-95 23.088787257530356 26.90859936467885 26.58348165003267 23.419131727758128 96-97 23.085263989806098 26.84787006739763 26.672617315035012 23.39424862776126 98-99 22.961844182095003 27.017586704557694 26.219581705790034 23.800987407557265 100-101 22.760143215296967 27.190269434366265 26.184238747433895 23.865348602902877 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 174.0 1 133.5 2 112.5 3 152.0 4 152.5 5 156.5 6 289.0 7 570.0 8 939.5 9 1258.0 10 1309.0 11 1237.5 12 1291.0 13 1450.5 14 1682.0 15 1894.5 16 2117.5 17 2344.0 18 2626.0 19 2944.5 20 3367.5 21 3877.0 22 4613.0 23 5682.5 24 6905.5 25 8488.5 26 10693.5 27 13631.5 28 16559.0 29 19841.0 30 23869.0 31 27676.5 32 32554.5 33 36259.5 34 38411.0 35 41869.5 36 46437.0 37 50238.5 38 52609.5 39 55215.5 40 57744.0 41 60204.0 42 62312.5 43 65721.5 44 69982.0 45 73821.0 46 76910.5 47 82095.5 48 92006.5 49 93123.0 50 85137.0 51 81368.5 52 81358.0 53 81869.0 54 82348.0 55 78446.0 56 70564.5 57 64264.0 58 61282.0 59 55956.5 60 46751.5 61 38738.0 62 32106.0 63 25448.0 64 18537.0 65 13484.0 66 10323.0 67 8216.0 68 6462.5 69 5150.0 70 4104.5 71 2951.5 72 2260.0 73 1913.0 74 1467.0 75 1069.5 76 785.0 77 519.0 78 350.5 79 234.5 80 155.0 81 104.0 82 74.0 83 48.0 84 25.5 85 10.5 86 6.0 87 3.5 88 2.5 89 2.0 90 3.0 91 2.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011715046806117778 2 0.009867674040537665 3 0.005226713190421778 4 4.05520850981E-4 5 0.005361886807415444 6 0.0 7 4.5057872331222227E-4 8 0.001847372765580111 9 1.3517361699366668E-4 10-11 0.0 12-13 0.0 14-15 8.335706381276111E-4 16-17 0.004032679573644389 18-19 0.002005075318739389 20-21 4.7310765947783335E-4 22-23 1.3517361699366668E-4 24-25 3.8299191481538887E-4 26-27 9.011574466244443E-5 28-29 2.2528936165611108E-5 30-31 3.8299191481538887E-4 32-33 0.0038524480843195 34-35 8.335706381276111E-4 36-37 0.0011039178721149443 38-39 0.005384415743581056 40-41 4.5057872331222216E-5 42-43 0.0016446123400896111 44-45 0.005677291913734 46-47 0.0 48-49 0.0020951910634018334 50-51 0.0017572570209176666 52-53 6.983970211339444E-4 54-55 2.027604254905E-4 56-57 0.011805162550780223 58-59 0.0022078357442298887 60-61 0.0032892246801792223 62-63 1.5770255315927776E-4 64-65 0.0033568114886760553 66-67 0.0010138021274525 68-69 0.0022303646803955 70-71 0.0049338370202688326 72-73 0.003086464254688722 74-75 0.007479606806982889 76-77 0.0011264468082805557 78-79 0.006938912339008222 80-81 0.0025457697867140553 82-83 0.0037172744673258334 84-85 0.002680943403707722 86-87 8.786285104588333E-4 88-89 0.0023204804250579443 90-91 0.008583524679097832 92-93 0.002973819573860667 94-95 8.11041701962E-4 96-97 9.011574466244443E-5 98-99 9.462153189556667E-4 100-101 0.0023430093612235555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2219368.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.876965287520086 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4014303129771 44.023584651156035 2 13.273161317435223 15.098742710148352 3 4.0252235084640136 6.868274932962526 4 1.771701827530187 4.030760933770814 5 0.9132215671772206 2.597063568807674 6 0.5705188154194334 1.946962731629292 7 0.3689650128235344 1.46899271586715 8 0.25274862824873634 1.1500459964297358 9 0.19486561752427295 0.9975028467293314 >10 1.097105032079882 11.534446914430394 >50 0.0853228189106456 3.2936867982038502 >100 0.04177566761969053 4.209254846506432 >500 0.002692714177256936 1.0555873613031337 >1k 0.0010295671854216731 0.9311276924765527 >5k 2.3759242740500148E-4 0.7939652995787198 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6494 0.2926058229189571 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6049 0.2725550697315632 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5083 0.22902916505960258 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3359 0.15134939316057544 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3517361699366668E-4 2 4.505787233122222E-5 0.0 0.0 0.0 1.3517361699366668E-4 3 4.505787233122222E-5 0.0 0.0 0.0 1.3517361699366668E-4 4 4.505787233122222E-5 0.0 0.0 0.0 1.3517361699366668E-4 5 4.505787233122222E-5 0.0 0.0 0.0 1.3517361699366668E-4 6 4.505787233122222E-5 0.0 0.0 0.0 1.802314893248889E-4 7 4.505787233122222E-5 0.0 0.0 0.0 1.802314893248889E-4 8 4.505787233122222E-5 0.0 0.0 0.0 1.802314893248889E-4 9 4.505787233122222E-5 0.0 0.0 0.0 2.252893616561111E-4 10-11 4.505787233122222E-5 0.0 0.0 2.252893616561111E-5 2.252893616561111E-4 12-13 4.505787233122222E-5 0.0 0.0 4.505787233122222E-5 2.478182978217222E-4 14-15 9.011574466244445E-5 0.0 0.0 4.505787233122222E-5 3.1540510631855557E-4 16-17 9.011574466244445E-5 0.0 0.0 4.505787233122222E-5 3.604629786497778E-4 18-19 9.011574466244445E-5 0.0 0.0 4.505787233122222E-5 4.05520850981E-4 20-21 9.011574466244445E-5 0.0 0.0 4.505787233122222E-5 4.05520850981E-4 22-23 9.011574466244445E-5 2.252893616561111E-5 0.0 2.252893616561111E-4 4.505787233122222E-4 24-25 1.3517361699366668E-4 4.505787233122222E-5 0.0 3.604629786497778E-4 4.956365956434444E-4 26-27 1.3517361699366668E-4 4.505787233122222E-5 0.0 6.308102126371111E-4 4.956365956434444E-4 28-29 1.802314893248889E-4 4.505787233122222E-5 0.0 0.001464380850764722 5.406944679746666E-4 30-31 1.802314893248889E-4 4.505787233122222E-5 0.0 0.0031540510631855555 6.082812764715E-4 32-33 1.802314893248889E-4 4.505787233122222E-5 0.0 0.0055871761690715555 8.11041701962E-4 34-35 2.252893616561111E-4 4.505787233122222E-5 0.0 0.008245590636613665 9.011574466244444E-4 36-37 2.7034723398733335E-4 4.505787233122222E-5 0.0 0.009732500423544 9.011574466244444E-4 38-39 3.8299191481538887E-4 4.505787233122222E-5 0.0 0.011309525955136778 9.011574466244444E-4 40-41 4.05520850981E-4 4.505787233122222E-5 0.0 0.012954138295226389 9.011574466244444E-4 42-43 4.2804978714661114E-4 4.505787233122222E-5 0.0 0.015567494890437277 9.011574466244444E-4 44-45 5.857523403058889E-4 4.505787233122222E-5 0.0 0.01923971148543189 9.236863827900556E-4 46-47 7.885127657963889E-4 4.505787233122222E-5 0.0 0.02318227531441383 9.912731912868889E-4 48-49 8.11041701962E-4 4.505787233122222E-5 0.0 0.027845765100695336 9.912731912868889E-4 50-51 8.11041701962E-4 4.505787233122222E-5 0.0 0.033455470205932494 9.912731912868889E-4 52-53 8.335706381276111E-4 4.505787233122222E-5 0.0 0.04670248467131183 0.0010138021274525 54-55 8.560995742932222E-4 4.505787233122222E-5 0.0 0.06355412892318894 0.0011039178721149443 56-57 8.560995742932222E-4 4.505787233122222E-5 0.0 0.0839202872169014 0.0011940336167773889 58-59 8.560995742932222E-4 4.505787233122222E-5 0.0 0.11266720976422116 0.001216562552943 60-61 8.560995742932222E-4 4.505787233122222E-5 0.0 0.1522730795433655 0.001216562552943 62-63 8.560995742932222E-4 4.505787233122222E-5 0.0 0.17872205060179294 0.001216562552943 64-65 8.560995742932222E-4 4.505787233122222E-5 0.0 0.20839265953190278 0.0012616204252742223 66-67 8.560995742932222E-4 4.505787233122222E-5 0.0 0.2690856135620591 0.0012616204252742223 68-69 8.560995742932222E-4 4.505787233122222E-5 0.0 0.3453235335464871 0.0012616204252742223 70-71 8.786285104588333E-4 4.505787233122222E-5 0.0 0.39993367481192843 0.0012841493614398334 72-73 9.236863827900556E-4 4.505787233122222E-5 0.0 0.44832582969566115 0.0014193229784335 74-75 9.462153189556667E-4 4.505787233122222E-5 0.0 0.5533332011635745 0.0014643808507647223 76-77 9.462153189556667E-4 4.505787233122222E-5 0.0 0.6301568734883084 0.0014869097869303334 78-79 9.687442551212777E-4 4.505787233122222E-5 0.0 0.6912553483694457 0.0014869097869303334 80-81 0.0010588599997837223 4.505787233122222E-5 0.0 0.7759416194159778 0.0015094387230959443 82-83 0.0010813889359493334 4.505787233122222E-5 0.0 0.9177838015146654 0.0015770255315927777 84-85 0.0011489757444461666 4.505787233122222E-5 0.0 1.0698315916963748 0.0015770255315927777 86-87 0.0012390914891086111 4.505787233122222E-5 0.0 1.267545535485778 0.0016671412762552223 88-89 0.0012616204252742223 4.505787233122222E-5 0.0 1.5288586660707013 0.0017797859570832777 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 170 3.1548589E-6 22.349936 4 TACATGG 8445 0.0 21.992815 2 GTACATG 8625 0.0 21.920832 1 GTATCAA 14965 0.0 21.744373 1 GAGTACT 8530 0.0 21.633467 12-13 ACATGGG 8280 0.0 21.39748 3 GTACTTT 8840 0.0 20.929974 14-15 GGTATCA 11775 0.0 20.897863 1 GTAAGGT 840 0.0 19.789007 4 AGAGTAC 13180 0.0 19.082045 10-11 AGTACTT 8980 0.0 19.0419 12-13 TAAGGTG 1040 0.0 18.723442 5 TCAACGC 17475 0.0 18.671309 4 ATCAACG 17495 0.0 18.652065 3 CAACGCA 17700 0.0 18.433962 5 TATCAAC 17685 0.0 18.425648 2 AACGCAG 17985 0.0 18.141846 6 ACTTTTT 10285 0.0 18.01107 16-17 GTATACG 135 0.009398161 17.59419 1 GTACGGT 135 0.009410363 17.590227 6 >>END_MODULE