FastQCFastQC Report
Thu 26 May 2016
SRR936658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313442
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62140.26860409727151147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51490.2225687957597381No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40530.17519349955607272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.12777497771718505No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28190.12185306569172687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG88500.025.0204262
GTACATG91050.024.8948351
ACATGGG87650.023.690393
GTATCAA128150.023.3981531
GGTATCA96750.022.1020431
GAGTACT72050.021.20033512-13
GTACTTT75700.020.27226814-15
CATGGGG52750.020.267624
TCAACGC148850.019.7918114
ATCAACG149350.019.6619193
CAACGCA149850.019.5658745
TATCAAC151700.019.4517232
AACGCAG152000.019.3203836
AGAGTAC117800.019.13880510-11
TAGCCCT15450.018.7604834
AGTACTT77200.018.73983412-13
GTGTAGC14450.018.4158171
GTATAGC9000.017.9517691
GTATTAC4002.1827873E-1117.819771
ACGCAGA166100.017.5929557
Produced by FastQC (version 0.11.5)