##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936658_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2313442 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.237985650818132 31.0 31.0 34.0 28.0 34.0 2 31.29130490412122 31.0 31.0 34.0 28.0 34.0 3 31.331354319667405 31.0 31.0 34.0 28.0 34.0 4 34.969990602746904 37.0 35.0 37.0 32.0 37.0 5 34.88961210179464 37.0 35.0 37.0 32.0 37.0 6 34.996824644836565 37.0 35.0 37.0 32.0 37.0 7 34.823028630067235 37.0 35.0 37.0 32.0 37.0 8 34.80830381742875 37.0 35.0 37.0 32.0 37.0 9 36.30204128739774 39.0 37.0 39.0 32.0 39.0 10-11 36.16847234553535 39.0 35.0 39.0 31.5 39.0 12-13 36.06352331288184 39.0 35.0 39.0 30.5 39.0 14-15 37.12285373914712 39.0 36.0 41.0 31.0 41.0 16-17 37.05664308852351 39.0 36.0 41.0 31.0 41.0 18-19 36.998336677556644 39.0 36.0 41.0 31.0 41.0 20-21 36.88958249223452 39.0 36.0 41.0 30.0 41.0 22-23 36.78007963891034 39.0 36.0 41.0 30.0 41.0 24-25 36.625059975568874 39.0 36.0 41.0 30.0 41.0 26-27 36.41367127423121 39.0 35.5 40.0 29.5 41.0 28-29 36.52928493560677 39.0 36.0 41.0 30.0 41.0 30-31 36.464839619925634 39.0 36.0 41.0 30.0 41.0 32-33 36.31845298045077 39.0 35.0 40.0 30.0 41.0 34-35 36.15250717329417 39.0 35.0 40.0 29.5 41.0 36-37 35.91739105627027 38.0 35.0 40.0 28.5 41.0 38-39 35.721164394871366 38.0 35.0 40.0 27.0 41.0 40-41 35.56550672115402 38.0 35.0 40.0 27.0 41.0 42-43 35.306325163976446 38.0 34.0 40.0 26.5 41.0 44-45 35.0436827463148 38.0 34.0 40.0 25.5 41.0 46-47 34.72298916506227 38.0 33.5 40.0 24.5 41.0 48-49 34.472195758527775 38.0 33.0 40.0 24.0 41.0 50-51 33.781663858441235 37.5 32.5 39.5 23.5 40.5 52-53 33.97039886887158 37.5 33.0 39.5 23.5 40.5 54-55 34.31851544149367 38.0 33.0 40.0 23.5 41.0 56-57 34.22669360200083 38.0 33.5 40.0 23.0 41.0 58-59 33.974222176306995 37.5 33.0 40.0 23.0 41.0 60-61 33.64130395315725 37.0 33.0 40.0 22.0 41.0 62-63 33.30573535018384 36.5 32.5 40.0 21.5 41.0 64-65 32.98450404202915 36.0 32.0 39.0 20.5 41.0 66-67 32.60432831253172 36.0 32.0 39.0 19.5 41.0 68-69 32.21284605362918 35.0 31.0 39.0 18.5 40.0 70-71 31.808597535620088 35.0 31.0 38.0 17.5 40.0 72-73 31.3946545882715 35.0 31.0 37.0 14.5 39.5 74-75 30.96938112129027 35.0 30.0 37.0 12.0 39.0 76-77 30.560029600915 34.5 30.0 36.0 8.0 39.0 78-79 30.113319893042487 34.0 29.5 36.0 7.0 38.5 80-81 29.713470015673614 34.0 29.0 35.0 4.5 37.0 82-83 29.309966059231222 34.0 29.0 35.0 2.0 37.0 84-85 28.927844527764258 34.0 29.0 35.0 2.0 36.0 86-87 28.58898558943773 34.0 29.0 35.0 2.0 36.0 88-89 28.212147095107635 34.0 27.5 35.0 2.0 35.5 90-91 27.85645976860453 34.0 27.0 35.0 2.0 35.0 92-93 27.480324123103152 33.0 26.5 35.0 2.0 35.0 94-95 27.047669230523177 33.0 25.5 35.0 2.0 35.0 96-97 26.45486163041909 33.0 25.0 35.0 2.0 35.0 98-99 25.768038489834627 33.0 23.5 35.0 2.0 35.0 100-101 23.65402633824405 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29630867904133495 1101 2 0.3386791656046775 1101 3 0.42214204777934583 1101 4 0.4220944324719369 1101 5 0.5344611085768562 1101 6 0.27018504610350647 1101 7 0.3512238436489312 1101 8 0.358982706674837 1101 9 0.5035813091236676 1101 10-11 0.531031670621168 1101 12-13 0.47184344582951354 1101 14-15 0.6317978124532218 1101 16-17 0.7654169842042009 1101 18-19 0.7847768675805256 1101 20-21 0.6993043282673881 1101 22-23 0.6237887338315673 1101 24-25 0.6005430302776489 1101 26-27 0.6159569419838036 1101 28-29 0.5931029710533551 1101 30-31 0.563691815230321 1101 32-33 0.4461241627217589 1101 34-35 0.5224536070373205 1101 36-37 0.5616076064646478 1101 38-39 0.5220352776698576 1101 40-41 0.4891281595034158 1101 42-43 0.34924186908169474 1101 44-45 0.4491009126242247 1101 46-47 0.3746920610867477 1101 48-49 0.385878036514832 1101 50-51 0.5207148931796794 1101 52-53 0.5626961741360148 1101 54-55 0.784384050318323 1101 56-57 0.5651152705515656 1101 58-59 0.602171866413002 1101 60-61 0.44947934538956247 1101 62-63 0.5849372890837756 1101 64-65 0.6655631902830947 1101 66-67 0.7189645384421368 1101 68-69 0.5936296384123665 1101 70-71 0.4083993945427373 1101 72-73 0.5268054148817214 1101 74-75 0.4503149212123887 1101 76-77 0.4055032938239407 1101 78-79 0.3408802704571876 1101 80-81 0.23568939250277765 1101 82-83 0.26359768144984486 1101 84-85 0.33506289906583575 1101 86-87 0.2386771315259395 1101 88-89 0.20852516676549726 1101 90-91 0.25974598644807045 1101 92-93 0.15042913098555744 1101 94-95 -0.0015094215321092008 1101 96-97 0.10965615625222824 1101 98-99 0.06334262979265048 1101 100-101 0.2065650486521342 1102 1 0.21179292908213654 1102 2 0.1891697809139643 1102 3 0.23588580491987088 1102 4 0.32269635837368327 1102 5 0.2240964929411433 1102 6 0.35600518646583623 1102 7 0.07041039497910617 1102 8 0.12691024054132782 1102 9 0.34526500424362894 1102 10-11 0.4181986365098638 1102 12-13 0.4662997866161973 1102 14-15 0.3242574174801618 1102 16-17 0.17191347514593502 1102 18-19 0.25862083689666804 1102 20-21 0.03757134311968002 1102 22-23 -0.17482853399933873 1102 24-25 -0.026965048716959927 1102 26-27 0.17855397152518293 1102 28-29 0.15028658456731137 1102 30-31 0.09633552340724094 1102 32-33 0.07643687693752099 1102 34-35 0.014153296934495074 1102 36-37 -0.08815997962969391 1102 38-39 -0.22968399766880054 1102 40-41 -0.40275236930349934 1102 42-43 -0.16521381885693387 1102 44-45 -0.12765051548088735 1102 46-47 0.019660561168358015 1102 48-49 0.04293630320326258 1102 50-51 0.029686983925564903 1102 52-53 -0.0829176662426292 1102 54-55 -0.3269549410314383 1102 56-57 -0.08796062303419916 1102 58-59 -0.1106843438040741 1102 60-61 -0.6251310675368273 1102 62-63 -0.4525032201377073 1102 64-65 -0.24435716225484327 1102 66-67 -0.5252264196123804 1102 68-69 -0.1653365767010655 1102 70-71 -0.13337308791197344 1102 72-73 -0.2658294798465306 1102 74-75 -0.36935604337060823 1102 76-77 -0.3229226895284327 1102 78-79 -0.2750710923631168 1102 80-81 -0.3145799073176896 1102 82-83 -0.3919720916854601 1102 84-85 -0.6013803847598531 1102 86-87 -0.5765237824613294 1102 88-89 -0.6301577122944018 1102 90-91 -0.5932535239158909 1102 92-93 -0.5326676537377963 1102 94-95 -0.5070341481519876 1102 96-97 -0.6683125070413887 1102 98-99 -0.7701305218313124 1102 100-101 -0.19519307114548212 1103 1 0.2374848021085647 1103 2 0.38430423329887375 1103 3 0.34039502878558636 1103 4 0.26088017090484783 1103 5 0.34041425926987756 1103 6 0.15443962223116614 1103 7 0.14639909366616166 1103 8 0.03498203711769321 1103 9 0.28860620417714955 1103 10-11 0.3889628046999931 1103 12-13 0.3607465208691423 1103 14-15 0.35917515056973315 1103 16-17 0.43400687717353037 1103 18-19 0.372069066911628 1103 20-21 0.33055754887006117 1103 22-23 0.3532233190835896 1103 24-25 0.33384299515872584 1103 26-27 0.36951757464966306 1103 28-29 0.38218886144947817 1103 30-31 0.35469217954817367 1103 32-33 0.34453626201314336 1103 34-35 0.20183420103953154 1103 36-37 0.4351981722732461 1103 38-39 0.3873118474367345 1103 40-41 0.31677430511896887 1103 42-43 0.41249434135578866 1103 44-45 0.4975906345938981 1103 46-47 0.415362405941508 1103 48-49 0.5466564295120548 1103 50-51 -0.026073694153218696 1103 52-53 0.2171813864414318 1103 54-55 0.47204023929097616 1103 56-57 0.4880932243980709 1103 58-59 0.5284165370335572 1103 60-61 0.6988506509275254 1103 62-63 0.5189872236050235 1103 64-65 0.6031286280060826 1103 66-67 0.5926259985361995 1103 68-69 0.40250553730924565 1103 70-71 0.43936083264643955 1103 72-73 0.38204387897015835 1103 74-75 0.25608919512033523 1103 76-77 0.21925547234625498 1103 78-79 0.35361662576884 1103 80-81 0.5115626886603728 1103 82-83 0.500465335603888 1103 84-85 0.3011537684726555 1103 86-87 0.4231365133671545 1103 88-89 0.534809879528769 1103 90-91 0.5055811112843713 1103 92-93 0.5188719839554068 1103 94-95 0.4946028802674931 1103 96-97 0.5335977472381508 1103 98-99 0.39345432111367984 1103 100-101 0.45131050206375534 1104 1 0.2789822435158307 1104 2 0.2890899117576353 1104 3 0.23356905420432028 1104 4 0.2636629659789733 1104 5 0.22341147181534637 1104 6 0.1164222506610102 1104 7 0.22563242033006503 1104 8 0.3039032894714069 1104 9 0.3645336426516934 1104 10-11 0.4193050832864742 1104 12-13 0.34316308224110514 1104 14-15 0.39951978629574825 1104 16-17 0.12316752993672964 1104 18-19 0.2539115551738931 1104 20-21 0.36377115230600054 1104 22-23 0.3239268155985897 1104 24-25 0.3362013680746401 1104 26-27 0.2721798404089242 1104 28-29 0.16202380686524975 1104 30-31 0.22519977310057726 1104 32-33 0.1453811320826759 1104 34-35 0.16449553490642188 1104 36-37 0.18103124294083983 1104 38-39 0.14097212881340937 1104 40-41 0.006411730464790821 1104 42-43 -0.062100148419432344 1104 44-45 -0.05504100205578055 1104 46-47 0.1787573309652828 1104 48-49 0.029425876598388356 1104 50-51 0.02146741189339707 1104 52-53 0.18715266222949367 1104 54-55 0.3683761207104155 1104 56-57 0.31963798675181465 1104 58-59 0.3185673939634128 1104 60-61 0.31695738823528785 1104 62-63 0.2828706383875712 1104 64-65 0.3004114080054805 1104 66-67 0.19723139693389413 1104 68-69 0.22877763891839464 1104 70-71 0.2825823434632433 1104 72-73 -0.008681599241377569 1104 74-75 0.00797038432463637 1104 76-77 0.1136789187583318 1104 78-79 0.1790592061240055 1104 80-81 0.17672444050839786 1104 82-83 0.11478557493577668 1104 84-85 0.21550703516654934 1104 86-87 0.114963337614995 1104 88-89 0.12133608626086456 1104 90-91 0.054925977242618274 1104 92-93 -0.05484919932863974 1104 94-95 0.01081033869549941 1104 96-97 0.13981874551415174 1104 98-99 -0.011143197311753283 1104 100-101 -0.0055165003399757495 1105 1 0.17584808818166664 1105 2 0.32926024183718994 1105 3 0.23020000494055637 1105 4 0.23343831721120978 1105 5 0.3755966136016724 1105 6 0.2644403917611129 1105 7 0.4043678535235955 1105 8 0.11639650247810351 1105 9 0.3999057461335127 1105 10-11 0.3789752766312162 1105 12-13 0.2387997349045392 1105 14-15 0.3436734205139018 1105 16-17 0.2907170742771825 1105 18-19 0.2446717211659859 1105 20-21 -0.1536218861008507 1105 22-23 -0.27751933277205154 1105 24-25 -0.2455460119327313 1105 26-27 -0.10759806074289457 1105 28-29 -0.0012224994468965633 1105 30-31 0.024307116470986045 1105 32-33 -0.08896685319658104 1105 34-35 -0.10610402026480159 1105 36-37 -0.13658739370215756 1105 38-39 -0.32244974386799186 1105 40-41 -0.36061316152607503 1105 42-43 -0.18595467451246606 1105 44-45 -0.0782660220827367 1105 46-47 -0.34999855559655657 1105 48-49 -0.10749414461967177 1105 50-51 -0.08394255377223203 1105 52-53 0.10118327490950918 1105 54-55 0.0961130770331593 1105 56-57 0.19649954175360307 1105 58-59 0.10072566067760391 1105 60-61 0.1542310864260088 1105 62-63 -0.1122054299491353 1105 64-65 -0.0786708796334068 1105 66-67 -0.28547470450912726 1105 68-69 -0.06346716586919143 1105 70-71 0.1801595076683995 1105 72-73 0.15285338980562102 1105 74-75 0.03204651463263275 1105 76-77 0.1836345552745442 1105 78-79 0.07508900692326037 1105 80-81 0.1758258568082134 1105 82-83 0.25936779179094316 1105 84-85 -0.05164728585269174 1105 86-87 0.2198486793582326 1105 88-89 -0.03689367257984699 1105 90-91 0.06136253427695948 1105 92-93 -0.08133197614273513 1105 94-95 0.012342209278976668 1105 96-97 0.07375230490055529 1105 98-99 -0.22944758323028225 1105 100-101 -0.08602452562198692 1106 1 0.023368215382152613 1106 2 0.017871321985648336 1106 3 -0.07012182974717263 1106 4 0.12272293832561587 1106 5 0.24252516535737811 1106 6 0.17948461527963389 1106 7 0.39165399741089146 1106 8 0.38472757406317015 1106 9 0.42390150926271275 1106 10-11 0.45140547943365306 1106 12-13 0.5066446190776688 1106 14-15 0.3134487083044135 1106 16-17 0.25901640176829943 1106 18-19 0.25937256883502613 1106 20-21 0.2691155252206343 1106 22-23 0.22738721603599998 1106 24-25 0.12160104770605074 1106 26-27 0.21367089503713288 1106 28-29 0.11305188924707466 1106 30-31 0.07541201146040066 1106 32-33 0.061372689181268925 1106 34-35 -0.16260423169551075 1106 36-37 -0.04074035570774015 1106 38-39 -0.06724005591848936 1106 40-41 -0.046008696180365405 1106 42-43 0.014261651240616402 1106 44-45 -0.30959325547805605 1106 46-47 -0.13160877291901585 1106 48-49 -0.15746366852019378 1106 50-51 -0.04891516509183447 1106 52-53 -0.23261383477083797 1106 54-55 -7.283045264259158E-4 1106 56-57 -0.12399497903589918 1106 58-59 -0.14409590891082047 1106 60-61 -0.08019794156481908 1106 62-63 -0.17931263622705984 1106 64-65 -0.1560858488093544 1106 66-67 -0.1631322587188393 1106 68-69 -0.26914486172863405 1106 70-71 -0.10467805044555334 1106 72-73 0.09515256454764653 1106 74-75 -0.158663833558645 1106 76-77 -0.4174640439749169 1106 78-79 -0.1958061411401566 1106 80-81 -0.10882563049031901 1106 82-83 -0.008630652409337358 1106 84-85 0.025957661330917148 1106 86-87 0.07368673641326495 1106 88-89 -0.040313940203350995 1106 90-91 -0.01909164069802216 1106 92-93 -0.05729072739819685 1106 94-95 -0.2278103393014277 1106 96-97 -0.1616518255766657 1106 98-99 -0.08900016696067681 1106 100-101 0.24934644532560313 1107 1 0.47992120997906085 1107 2 0.5441351902656102 1107 3 0.5459219599694123 1107 4 0.518449530631635 1107 5 0.5713859071598151 1107 6 0.4869664240809968 1107 7 0.6075850864444305 1107 8 0.6893145513356984 1107 9 0.717862953139857 1107 10-11 0.8241202426284033 1107 12-13 0.770432239249601 1107 14-15 0.8449075130824539 1107 16-17 0.9331196284572556 1107 18-19 0.9825321874963535 1107 20-21 1.0355084194276998 1107 22-23 1.1225410123243122 1107 24-25 1.1054594254704142 1107 26-27 1.0174427252189062 1107 28-29 0.9207548619877883 1107 30-31 0.8597481776745823 1107 32-33 0.9879154098768623 1107 34-35 0.8015397040264531 1107 36-37 0.9083833890475148 1107 38-39 0.9260436227031761 1107 40-41 0.8448667859198551 1107 42-43 0.8740448288051468 1107 44-45 0.7352312853186973 1107 46-47 0.8646704690425082 1107 48-49 0.8415293850735921 1107 50-51 0.9320104673255329 1107 52-53 0.7701657684870753 1107 54-55 0.9245331521373359 1107 56-57 0.9603435660845179 1107 58-59 0.8901161081340447 1107 60-61 0.7359817431706261 1107 62-63 0.8338207847293191 1107 64-65 0.7095100374670409 1107 66-67 0.7910975852546471 1107 68-69 0.9202498999198312 1107 70-71 0.9832504128702233 1107 72-73 0.9549268212137889 1107 74-75 0.7692040122525441 1107 76-77 0.9241004288445893 1107 78-79 0.9051958987719431 1107 80-81 0.8958407427779171 1107 82-83 0.8606941693957175 1107 84-85 0.8638693979622083 1107 86-87 0.940737472536064 1107 88-89 1.0823383767026407 1107 90-91 1.143721445665193 1107 92-93 1.0973125305138964 1107 94-95 1.1396849549246681 1107 96-97 1.2054048578117325 1107 98-99 1.4647225982799341 1107 100-101 1.7815508885277325 1108 1 0.022917399593705312 1108 2 0.13142882294719982 1108 3 -0.019270923656211636 1108 4 0.0914285186864916 1108 5 0.31101014559673246 1108 6 0.23859234840784183 1108 7 0.3567147286382806 1108 8 0.10353976155794697 1108 9 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2316 78-79 -2.6805668836089076 2316 80-81 -2.7321724999102805 2316 82-83 -2.652183371146741 2316 84-85 -2.662052101931568 2316 86-87 -2.7578714255589745 2316 88-89 -3.027767139903826 2316 90-91 -3.0513705272828986 2316 92-93 -3.1048301116502586 2316 94-95 -3.162926404044242 2316 96-97 -3.4420785003073853 2316 98-99 -3.9084428113935985 2316 100-101 -4.3868445862969985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5953.0 3 2481.0 4 2513.0 5 2771.0 6 3053.0 7 3722.0 8 4424.0 9 5571.0 10 6816.0 11 8623.0 12 10141.0 13 11834.0 14 13156.0 15 15375.0 16 17940.0 17 21415.0 18 19459.0 19 15245.0 20 15976.0 21 17259.0 22 19018.0 23 21793.0 24 24796.0 25 28502.0 26 33648.0 27 39778.0 28 46918.0 29 56489.0 30 67844.0 31 82513.0 32 103047.0 33 130347.0 34 171108.0 35 230111.0 36 316624.0 37 398368.0 38 294639.0 39 44150.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.562178492021395 17.859664185605347 14.264956943491548 23.31320037888171 2 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22.99592376621524 25.86757211216343 27.957625323656423 23.17887879796491 46-47 23.328975377513597 26.181040968603046 27.587438107697633 22.902545546185724 48-49 23.327350160421798 26.14197734319026 27.440891402710044 23.0897810936779 50-51 23.116303939994918 26.411167266936513 27.523943072632363 22.948585720436206 52-53 23.385739860090286 26.419861845263327 27.154726086329745 23.03967220831665 54-55 23.049429256756433 26.275910602073928 27.34259334905919 23.332066792110442 56-57 23.138875224454544 26.509324427484216 27.120549705064352 23.23125064299689 58-59 23.3974521767115 26.24652214405159 27.14371333972944 23.21231233950747 60-61 23.195360474032885 26.274041183941048 27.565243063750472 22.965355278275595 62-63 22.94588125855608 26.404645430798563 27.576377412939234 23.073095897706118 64-65 22.933580994685684 26.524980396598213 27.274988825844847 23.266449782871252 66-67 22.904977461627706 26.461060574361067 27.112594169244414 23.52136779476681 68-69 22.968507044664552 26.37518258461573 27.37197895138181 23.28433141933791 70-71 23.18130954488401 26.228313001316128 27.135467022533703 23.454910431266153 72-73 23.035077416798853 26.343843972032822 27.268842713038577 23.35223589812975 74-75 22.950524525061507 26.765896717722587 26.911700787398168 23.371877969817735 76-77 23.12053561490262 26.53517355676989 26.94055469089905 23.403736137428442 78-79 23.05471267548691 26.580136184435972 27.044533975303466 23.320617164773655 80-81 23.20113880911436 26.543353325904125 26.901425348879187 23.354082516102324 82-83 23.2048922842432 26.581132959590757 27.018506006638837 23.1954687495272 84-85 23.147549147493383 26.688040483013953 26.864544141368924 23.299866228123737 86-87 23.16112049048747 26.729781668147602 26.81118086984752 23.297916971517413 88-89 23.1004955200886 26.66972719377762 26.56049058347566 23.66928670265812 90-91 23.00477845473934 26.546502446507443 26.889123725167195 23.55959537358602 92-93 23.192237075951443 26.481757119839667 27.04246462290151 23.28354118130739 94-95 22.90075642906346 26.40790540454376 27.156575001939864 23.53476316445291 96-97 22.88140373840623 26.32411523384437 27.24158806725526 23.552892960494137 98-99 22.7420846446148 26.522271860804057 26.8988270777335 23.836816416847643 100-101 22.7790317060182 26.30738325296253 27.074168960530127 23.839416080489148 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 84.0 1 75.5 2 101.0 3 154.0 4 154.5 5 165.5 6 288.5 7 581.0 8 972.0 9 1217.0 10 1219.5 11 1199.0 12 1311.0 13 1432.5 14 1605.5 15 1794.5 16 1960.0 17 2155.0 18 2413.0 19 2718.5 20 3106.0 21 3674.0 22 4420.0 23 5435.5 24 6718.0 25 8394.5 26 10869.5 27 14023.0 28 16811.5 29 19975.0 30 23933.0 31 28113.0 32 33221.0 33 36975.0 34 39459.5 35 43000.5 36 47792.0 37 52175.5 38 54883.5 39 57398.5 40 59918.0 41 62732.5 42 65518.5 43 68988.5 44 73015.0 45 77418.5 46 81154.5 47 86311.0 48 95334.0 49 96279.5 50 89278.5 51 85860.0 52 85837.5 53 86203.5 54 86241.5 55 81857.5 56 74302.0 57 68121.5 58 64228.0 59 58289.5 60 49313.0 61 41382.5 62 34272.5 63 27275.0 64 20036.5 65 14567.0 66 10917.0 67 8425.0 68 6536.0 69 5258.0 70 4222.0 71 3067.5 72 2342.5 73 1983.5 74 1525.0 75 1070.0 76 800.5 77 528.0 78 348.5 79 231.0 80 157.0 81 115.5 82 69.5 83 47.5 84 34.5 85 23.5 86 14.5 87 10.5 88 10.0 89 5.0 90 3.5 91 5.0 92 5.0 93 3.5 94 2.0 95 2.0 96 2.5 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013788977635920848 2 0.005878686390235848 3 0.008256096327463582 4 0.032808257133742705 5 0.04413337356199118 6 0.05610687451857449 7 0.026237960579949704 8 0.02381732500749965 9 0.013529623824586914 10-11 0.043722730027379114 12-13 0.04076177401465003 14-15 0.0179386386172638 16-17 0.02243410468038533 18-19 0.028464080793899307 20-21 0.02243410468038533 22-23 0.01400510581203246 24-25 0.003177084188840697 26-27 0.002636763748561667 28-29 0.0015128972327812843 30-31 0.006354168377681394 32-33 0.0012319306038361887 34-35 0.00179386386172638 36-37 0.005576106943679591 38-39 0.005900299207847009 40-41 0.003695791811508566 42-43 8.42899886835287E-4 44-45 0.001383220327114317 46-47 0.002096443308282637 48-49 4.106435346120629E-4 50-51 0.0016425741384482515 52-53 9.293511572799318E-4 54-55 0.0023774099372277325 56-57 0.002420635572450055 58-59 0.002399022754838894 60-61 6.916101635571585E-4 62-63 0.0012535434214473499 64-65 0.002939343195117924 66-67 0.00233418430200541 68-69 0.0056625582141242355 70-71 0.009444801296077446 72-73 0.0037822430819532105 74-75 0.0027448278366174732 76-77 0.0027448278366174732 78-79 0.0332621263035771 80-81 0.021591204793550044 82-83 0.0036741789938974046 84-85 0.007586098981517584 86-87 0.006548683736181845 88-89 0.004970948050567077 90-91 0.010655119082302475 92-93 0.012665111120140467 94-95 0.0059867504782916536 96-97 0.003306761094507664 98-99 0.021656043246383528 100-101 0.040264679209593326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2313442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.361169751449474 #Duplication Level Percentage of deduplicated Percentage of total 1 79.10486879589796 46.95757544759437 2 12.329578584971523 14.637964146926615 3 3.773865414755613 6.720631965132966 4 1.6068524842638283 3.8153857233569366 5 0.8385044312319926 2.4887301939852455 6 0.5113441360029126 1.8212391635206295 7 0.32830720678827 1.3642089882947905 8 0.2275548454187796 1.0806337445335217 9 0.18306314445700125 0.9780158154011551 >10 0.9803788789846483 10.816102239185183 >50 0.07519678367889727 3.043233024598766 >100 0.03727970686773517 3.968223428676793 >500 0.0023313357679330617 0.9656219344641596 >1k 7.285424274787459E-4 0.851017038085777 >5k 1.457084854957492E-4 0.4914171462429457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6214 0.26860409727151147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5149 0.2225687957597381 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4053 0.17519349955607272 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2956 0.12777497771718505 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2819 0.12185306569172687 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.645127044464482E-5 2 0.0 0.0 0.0 0.0 8.645127044464482E-5 3 0.0 0.0 0.0 0.0 8.645127044464482E-5 4 0.0 0.0 0.0 0.0 8.645127044464482E-5 5 0.0 0.0 0.0 0.0 8.645127044464482E-5 6 0.0 0.0 0.0 0.0 1.2967690566696724E-4 7 8.645127044464482E-5 0.0 0.0 0.0 1.2967690566696724E-4 8 8.645127044464482E-5 0.0 0.0 0.0 1.2967690566696724E-4 9 8.645127044464482E-5 0.0 0.0 0.0 1.7290254088928963E-4 10-11 8.645127044464482E-5 0.0 0.0 0.0 1.7290254088928963E-4 12-13 8.645127044464482E-5 0.0 0.0 0.0 4.106435346120629E-4 14-15 1.9451535850045085E-4 0.0 0.0 0.0 5.403204402790301E-4 16-17 2.593538113339344E-4 0.0 0.0 4.322563522232241E-5 6.483845283348362E-4 18-19 3.2419226416741803E-4 0.0 0.0 8.645127044464482E-5 6.483845283348362E-4 20-21 3.4580508177857927E-4 0.0 0.0 1.2967690566696724E-4 7.564486163906421E-4 22-23 3.4580508177857927E-4 0.0 0.0 3.241922641674181E-4 8.212870692241258E-4 24-25 4.322563522232241E-4 0.0 0.0 3.890307170009017E-4 8.645127044464482E-4 26-27 4.322563522232241E-4 0.0 0.0 7.34835798779481E-4 8.861255220576094E-4 28-29 5.18707622667869E-4 0.0 0.0 0.0013399946918919948 9.077383396687706E-4 30-31 5.18707622667869E-4 0.0 0.0 0.0027448278366174732 9.293511572799318E-4 32-33 5.18707622667869E-4 0.0 0.0 0.0053383659499568175 9.941896101134154E-4 34-35 5.835460755013526E-4 0.0 0.0 0.0074780348934617765 9.941896101134154E-4 36-37 6.483845283348362E-4 0.0 0.0 0.008753191132520289 9.941896101134154E-4 38-39 6.916101635571585E-4 0.0 0.0 0.009985121736356475 0.0010806408805580602 40-41 6.916101635571585E-4 0.0 0.0 0.011735759962860533 0.0011670921510027051 42-43 7.780614340018034E-4 0.0 0.0 0.014199621170532912 0.0011670921510027051 44-45 8.645127044464482E-4 0.0 0.0 0.016577031107760647 0.0011887049686138663 46-47 9.077383396687706E-4 0.0 0.0 0.02025121010165805 0.0012319306038361885 48-49 9.50963974891093E-4 0.0 0.0 0.024336032630167516 0.0012535434214473499 50-51 9.50963974891093E-4 0.0 0.0 0.029371819133568077 0.0012535434214473499 52-53 9.50963974891093E-4 0.0 0.0 0.040869838102705836 0.0013399946918919948 54-55 9.941896101134154E-4 0.0 0.0 0.055501715625461974 0.001383220327114317 56-57 0.0010806408805580602 0.0 0.0 0.07320261324900301 0.0014048331447254784 58-59 0.0010806408805580602 0.0 0.0 0.10026186089817682 0.0014264459623366396 60-61 0.0010806408805580602 0.0 0.0 0.13603107404464862 0.001469671597558962 62-63 0.0010806408805580602 0.0 0.0 0.15989162468737061 0.001469671597558962 64-65 0.0010806408805580602 0.0 0.0 0.18684280824848862 0.0015345100503924456 66-67 0.0010806408805580602 0.0 0.0 0.2422796854211171 0.0015561228680036068 68-69 0.0010806408805580602 0.0 0.0 0.31111650951266556 0.001577735685614768 70-71 0.0011238665157803827 0.0 0.0 0.36203630780456136 0.0016209613208370904 72-73 0.0012319306038361887 0.0 0.0 0.404894525127494 0.0019235407673933473 74-75 0.0012967690566696723 0.0 0.0 0.5043134861388356 0.002074830490671476 76-77 0.0012967690566696723 0.0 0.0 0.5725235385196603 0.002074830490671476 78-79 0.0012967690566696723 0.0 0.0 0.6240268828870574 0.002096443308282637 80-81 0.001383220327114317 0.0 0.0 0.6968620782366708 0.002139668943504959 82-83 0.001383220327114317 0.0 0.0 0.8301267116270907 0.0022909586667830876 84-85 0.0014480587799478007 0.0 0.0 0.9691619673196907 0.0022909586667830876 86-87 0.0015128972327812843 0.0 0.0 1.1605218544489122 0.0023774099372277325 88-89 0.0015128972327812843 0.0 0.0 1.4226853320722976 0.0025286996605058606 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 8850 0.0 25.020426 2 GTACATG 9105 0.0 24.894835 1 ACATGGG 8765 0.0 23.69039 3 GTATCAA 12815 0.0 23.398153 1 GGTATCA 9675 0.0 22.102043 1 GAGTACT 7205 0.0 21.200335 12-13 GTACTTT 7570 0.0 20.272268 14-15 CATGGGG 5275 0.0 20.26762 4 TCAACGC 14885 0.0 19.791811 4 ATCAACG 14935 0.0 19.661919 3 CAACGCA 14985 0.0 19.565874 5 TATCAAC 15170 0.0 19.451723 2 AACGCAG 15200 0.0 19.320383 6 AGAGTAC 11780 0.0 19.138805 10-11 TAGCCCT 1545 0.0 18.760483 4 AGTACTT 7720 0.0 18.739834 12-13 GTGTAGC 1445 0.0 18.415817 1 GTATAGC 900 0.0 17.951769 1 GTATTAC 400 2.1827873E-11 17.81977 1 ACGCAGA 16610 0.0 17.592955 7 >>END_MODULE