FastQCFastQC Report
Thu 26 May 2016
SRR936658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313442
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68200.2947988322162388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.2750447169196375No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49650.21461527887883078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36280.1568226045865857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT9750.022.8933624
GTATCAA152350.022.3065011
GGTATCA117300.021.6879481
GAGTACT86750.021.64989712-13
GTACATG86800.021.1796951
GTACTTT89200.020.86892114-15
TAAGGTG11250.020.684765
ATACCGT3001.6734703E-1020.5792276
TACATGG86600.020.3474452
AGTACTT90450.020.21300912-13
GGTTGCG3102.6011548E-1019.915387
ACATGGG86250.019.8238453
AGAGTAC136650.019.30426210-11
TCAACGC177050.019.2326684
ATCAACG177450.019.1905613
CAACGCA177900.019.1403625
TACCGTC2251.6723152E-618.9962087
AACGCAG180400.018.9540886
TATCAAC180150.018.850622
TACTTTT100900.018.00192814-15
Produced by FastQC (version 0.11.5)