##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936658_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2313442 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.518161682895013 31.0 31.0 34.0 30.0 34.0 2 31.743920962790508 31.0 31.0 34.0 30.0 34.0 3 32.02304099259891 33.0 31.0 34.0 30.0 34.0 4 35.81934234789547 37.0 35.0 37.0 35.0 37.0 5 35.63349718730792 37.0 35.0 37.0 33.0 37.0 6 35.72764002728402 37.0 35.0 37.0 33.0 37.0 7 35.66832148806843 37.0 35.0 37.0 33.0 37.0 8 35.64159637457952 37.0 35.0 37.0 33.0 37.0 9 37.28524985713928 39.0 37.0 39.0 34.0 39.0 10-11 37.17580190037183 39.0 37.0 39.0 33.0 39.0 12-13 37.045407016903816 39.0 37.0 39.0 33.0 39.0 14-15 38.19499451466689 40.0 37.5 41.0 33.0 41.0 16-17 38.10268509000874 40.0 37.0 41.0 33.0 41.0 18-19 38.148062281224256 40.0 37.5 41.0 33.0 41.0 20-21 38.21203924714776 40.0 38.0 41.0 33.0 41.0 22-23 38.12712961898332 40.0 37.0 41.0 33.0 41.0 24-25 38.02709642169546 40.0 37.0 41.0 33.0 41.0 26-27 37.82170570950126 40.0 37.0 41.0 33.0 41.0 28-29 37.74530872180932 40.0 37.0 41.0 32.5 41.0 30-31 37.74768202531121 40.0 37.0 41.0 33.0 41.0 32-33 37.58555152884749 40.0 36.5 41.0 32.5 41.0 34-35 37.43309190375207 40.0 36.0 41.0 32.0 41.0 36-37 37.21547482063522 39.0 36.0 41.0 31.5 41.0 38-39 37.056950855046296 39.0 36.0 41.0 31.0 41.0 40-41 36.82558131995529 39.0 35.5 41.0 30.5 41.0 42-43 36.59476204720066 39.0 35.0 40.5 30.0 41.0 44-45 36.34063421516511 39.0 35.0 40.0 30.0 41.0 46-47 35.98040084860567 39.0 35.0 40.0 29.5 41.0 48-49 35.54714317454252 38.0 35.0 40.0 28.0 41.0 50-51 35.346050603386644 38.0 34.5 40.0 27.0 41.0 52-53 35.07096460598537 38.0 34.0 40.0 26.5 41.0 54-55 34.790589735986465 38.0 34.0 40.0 26.0 41.0 56-57 34.496763696690905 38.0 33.5 40.0 26.0 41.0 58-59 34.11907733152593 37.0 33.0 40.0 25.0 41.0 60-61 33.73797787020379 37.0 33.0 39.5 24.0 41.0 62-63 33.42533484738325 36.0 32.0 39.0 23.0 40.5 64-65 32.980165485021885 36.0 32.0 39.0 22.5 40.0 66-67 32.567033450590074 35.0 31.0 38.5 22.0 40.0 68-69 32.105384963184726 35.0 31.0 38.0 20.5 40.0 70-71 31.627084664322684 35.0 31.0 37.0 20.0 39.5 72-73 31.207927192469057 34.0 30.0 37.0 18.5 39.0 74-75 30.835904250030907 34.0 30.0 36.0 18.0 39.0 76-77 29.422052076516287 32.5 28.5 35.0 16.0 37.0 78-79 30.24009916825233 34.0 30.0 35.0 17.0 37.0 80-81 30.094002140533455 34.0 30.0 35.0 14.0 37.0 82-83 29.751747828560216 34.0 29.5 35.0 9.5 36.5 84-85 29.312853963920425 34.0 29.0 35.0 7.0 36.0 86-87 28.929946590405116 34.0 29.0 35.0 3.5 36.0 88-89 28.512057790945267 33.0 29.0 35.0 2.0 35.0 90-91 28.05808704086811 33.0 27.0 35.0 2.0 35.0 92-93 27.593597116331424 33.0 27.0 35.0 2.0 35.0 94-95 27.045811824977672 33.0 25.5 34.0 2.0 35.0 96-97 26.342040345078892 32.0 25.0 34.0 2.0 35.0 98-99 25.451415466650992 32.0 22.0 34.0 2.0 35.0 100-101 22.588837973893444 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36917070065944557 1101 2 0.3678091361737863 1101 3 0.174345158547478 1101 4 0.0963865367857295 1101 5 0.14752142592489292 1101 6 0.04349913466410271 1101 7 0.15492277774906427 1101 8 0.09089340132675261 1101 9 0.2404234191873016 1101 10-11 0.15925990389188627 1101 12-13 0.1017769785289957 1101 14-15 0.2664067038786513 1101 16-17 0.25271411681080735 1101 18-19 0.2258593824007349 1101 20-21 0.03207337947686284 1101 22-23 0.04255799564887042 1101 24-25 0.07714246720072282 1101 26-27 0.05380244556757674 1101 28-29 0.13961706570723464 1101 30-31 0.051732285045652304 1101 32-33 0.1082883518162916 1101 34-35 0.1910977707977537 1101 36-37 0.07634771744226754 1101 38-39 0.20059045619809268 1101 40-41 0.2426146117301471 1101 42-43 0.28293084369000354 1101 44-45 0.3807233516112589 1101 46-47 0.3182201572720018 1101 48-49 0.31854509230470285 1101 50-51 0.22953640420396226 1101 52-53 0.13726854645099706 1101 54-55 0.22997780130356205 1101 56-57 0.158127591581966 1101 58-59 0.1569775762145511 1101 60-61 0.14351190709515294 1101 62-63 0.019668160953798974 1101 64-65 0.042131590344403946 1101 66-67 0.04405256730270679 1101 68-69 -0.10137032073900798 1101 70-71 -0.0043605939328514864 1101 72-73 0.06017880146678678 1101 74-75 0.138610057277468 1101 76-77 0.4281940308199914 1101 78-79 0.40263936244052445 1101 80-81 0.24783956215707903 1101 82-83 0.1450546143484246 1101 84-85 0.20173119041156795 1101 86-87 0.1994331856300846 1101 88-89 0.004928020346703477 1101 90-91 0.03863220817212465 1101 92-93 0.2275378516779618 1101 94-95 -0.17160168740031523 1101 96-97 -0.12012000814738855 1101 98-99 0.08701718000465775 1101 100-101 0.15489293722400888 1102 1 0.4379612996475295 1102 2 0.511078599206261 1102 3 0.14127514059415702 1102 4 0.120966265853788 1102 5 0.1965555372354899 1102 6 0.1612148193384968 1102 7 0.09470537934853951 1102 8 -0.013402991687776478 1102 9 0.1341397567097431 1102 10-11 0.14353181510455926 1102 12-13 0.10854733922754178 1102 14-15 0.21456861019495221 1102 16-17 0.3559098080136778 1102 18-19 0.18851157760230564 1102 20-21 0.199939219202669 1102 22-23 0.041309750178001536 1102 24-25 0.18522831671923967 1102 26-27 0.013887642238046283 1102 28-29 0.03640178906287872 1102 30-31 0.12799897347391465 1102 32-33 0.3090456555538452 1102 34-35 0.30028921848830237 1102 36-37 0.2087299599753223 1102 38-39 0.19804696019678403 1102 40-41 0.2306622696377758 1102 42-43 0.21802926053347704 1102 44-45 0.09924726217108315 1102 46-47 0.1537837283508381 1102 48-49 0.19142513147559015 1102 50-51 0.16082349413377273 1102 52-53 0.013922700196708604 1102 54-55 0.2316324446853173 1102 56-57 0.17716105004451066 1102 58-59 0.15895178871658544 1102 60-61 0.2757044972697855 1102 62-63 0.17364857551231694 1102 64-65 0.170161294930665 1102 66-67 0.11253763340356926 1102 68-69 0.15417921410675461 1102 70-71 -0.05559219177845165 1102 72-73 -0.07072637235400947 1102 74-75 -0.3534434762626013 1102 76-77 -0.07184057012663914 1102 78-79 0.11563626141229122 1102 80-81 0.13464550307433143 1102 82-83 0.20934415245842786 1102 84-85 0.29000571301643063 1102 86-87 0.1578640653428316 1102 88-89 0.203863225177777 1102 90-91 0.2888701712862094 1102 92-93 0.39015152884460136 1102 94-95 0.030667285996123894 1102 96-97 -0.21512996408014473 1102 98-99 -0.40557887026464456 1102 100-101 -0.4304346292848855 1103 1 0.36591875352820225 1103 2 0.4044719846142719 1103 3 0.2382038426051274 1103 4 0.12751437953169642 1103 5 -0.05417938049760096 1103 6 0.01979484260590425 1103 7 0.013999379680932122 1103 8 0.010812398041323945 1103 9 -0.044288031224439806 1103 10-11 0.09109472020676179 1103 12-13 0.16652939008344703 1103 14-15 0.1269422183053095 1103 16-17 0.08848750454018983 1103 18-19 0.2082273828208514 1103 20-21 0.15259103904644178 1103 22-23 0.0774586615956494 1103 24-25 0.039893099833754775 1103 26-27 0.10845603180094798 1103 28-29 0.15160870280727323 1103 30-31 0.12555489835343536 1103 32-33 0.059363089491029086 1103 34-35 0.08027326369354881 1103 36-37 0.13435302794939474 1103 38-39 0.23383954786505967 1103 40-41 0.2795856533838261 1103 42-43 0.2581309723499956 1103 44-45 0.3118754822142904 1103 46-47 0.24519838681850814 1103 48-49 0.3894879534799145 1103 50-51 0.3010900721626939 1103 52-53 0.2550794228159532 1103 54-55 0.3285415355943968 1103 56-57 0.3810536875715158 1103 58-59 0.2759010990041091 1103 60-61 0.3288499185760898 1103 62-63 0.1725520157649285 1103 64-65 0.15826200957251046 1103 66-67 0.10641032743713907 1103 68-69 0.19082671286028585 1103 70-71 0.10319391425445446 1103 72-73 0.18755449851186867 1103 74-75 0.19896669491905072 1103 76-77 0.04639974896925381 1103 78-79 0.24909612513175006 1103 80-81 0.33066345221842397 1103 82-83 0.4037363744959883 1103 84-85 0.34200538062487595 1103 86-87 0.3231913048309494 1103 88-89 0.33338026024508594 1103 90-91 0.247775273496611 1103 92-93 0.5641728019042667 1103 94-95 0.3471967857468279 1103 96-97 -0.05630563301815528 1103 98-99 -0.13465382456080732 1103 100-101 -0.002041410072653349 1104 1 0.27466840064248643 1104 2 0.34269615406519804 1104 3 0.15194980326281993 1104 4 0.039645562569063486 1104 5 0.04622270365227621 1104 6 0.021503220592414607 1104 7 0.06682095792288578 1104 8 -0.05708651356319905 1104 9 -0.006115115853972952 1104 10-11 -0.08002059051082 1104 12-13 -0.018935555688635475 1104 14-15 0.10922201076739668 1104 16-17 -0.08109169123669346 1104 18-19 -0.0461544984505764 1104 20-21 -0.10878832611558664 1104 22-23 -0.11650843164017743 1104 24-25 0.011107150029864954 1104 26-27 0.05263958444876238 1104 28-29 -0.11488620330457167 1104 30-31 -0.1118594155134005 1104 32-33 -0.020334881450381204 1104 34-35 -0.002821180857480954 1104 36-37 -0.045493292035040156 1104 38-39 -0.08979152964919734 1104 40-41 0.034886339421063894 1104 42-43 0.06429639969117318 1104 44-45 0.09419056324781394 1104 46-47 0.015217148004644798 1104 48-49 0.01770072095357733 1104 50-51 0.044621231628660496 1104 52-53 -0.08565955413067172 1104 54-55 0.11384888192124265 1104 56-57 0.02020207498657811 1104 58-59 0.12112624694201912 1104 60-61 -0.11571420247125275 1104 62-63 -0.18749398956877883 1104 64-65 -0.23831328596071444 1104 66-67 -0.13980296902249023 1104 68-69 -0.17644461034465664 1104 70-71 -0.07704496539164296 1104 72-73 -0.10000795883349767 1104 74-75 -0.23757568288656472 1104 76-77 0.0517415479052552 1104 78-79 0.10360586751832912 1104 80-81 0.1005361940924594 1104 82-83 -0.04968024443403607 1104 84-85 0.06349310568373667 1104 86-87 0.04875462006546982 1104 88-89 0.002458563872067998 1104 90-91 0.017284645867100323 1104 92-93 0.10218928269494754 1104 94-95 0.08374252110584379 1104 96-97 -0.05647097539267065 1104 98-99 0.06579444307868343 1104 100-101 0.17584341351189536 1105 1 0.4008709421476162 1105 2 0.3779144664107328 1105 3 0.20605329759074564 1105 4 0.09661352341230867 1105 5 -0.07526547148654572 1105 6 -0.015666760969700988 1105 7 0.10111183296318416 1105 8 0.017892350815756686 1105 9 -0.02012645489435272 1105 10-11 -0.02596499601000346 1105 12-13 -0.013494305892599812 1105 14-15 0.054541444338440215 1105 16-17 0.07935311480169105 1105 18-19 0.11364792400144808 1105 20-21 0.08322346093742539 1105 22-23 0.03783362372576704 1105 24-25 -0.053997456370005636 1105 26-27 0.03227554860547599 1105 28-29 -0.04956022010732397 1105 30-31 -0.10975487904443781 1105 32-33 -0.038385654130024705 1105 34-35 -0.0889670300034453 1105 36-37 0.043123520150452066 1105 38-39 -0.032495874291434745 1105 40-41 -0.039985780832807905 1105 42-43 0.037511868179912256 1105 44-45 -0.04827354315177246 1105 46-47 0.16475976861698172 1105 48-49 0.254231488561814 1105 50-51 0.03613855601539484 1105 52-53 0.027389794346397878 1105 54-55 0.06642549463877145 1105 56-57 0.07180241247426267 1105 58-59 0.07971450116530576 1105 60-61 -0.014818617840354875 1105 62-63 -0.12193724859911015 1105 64-65 -2.0992580915901726E-4 1105 66-67 0.022620441001734548 1105 68-69 -0.14138158156325886 1105 70-71 -0.242783697277126 1105 72-73 -0.34308145905722043 1105 74-75 -0.2985294210345515 1105 76-77 -0.27498146715149474 1105 78-79 -0.172849690254548 1105 80-81 -0.3284103110395016 1105 82-83 -0.22402992965968238 1105 84-85 -0.13673534948572197 1105 86-87 -0.07808056871101599 1105 88-89 -0.10374928214803703 1105 90-91 -0.3469049174854675 1105 92-93 -0.23001925751730923 1105 94-95 -0.1471477931227092 1105 96-97 -0.3066366348837448 1105 98-99 -0.3001120519104532 1105 100-101 -0.2103090733845896 1106 1 0.35036480593906205 1106 2 0.29906983347743576 1106 3 0.11674947962992732 1106 4 0.0020139118314475013 1106 5 -0.003970123728741726 1106 6 0.04295439236267384 1106 7 0.05455240324221933 1106 8 0.07738959985753979 1106 9 0.02457521549668229 1106 10-11 -0.0699583587961925 1106 12-13 0.03377807583152759 1106 14-15 0.06694921248535479 1106 16-17 0.05860032123296577 1106 18-19 -0.06890818770236962 1106 20-21 -9.317486153292975E-6 1106 22-23 0.0421229836008834 1106 24-25 0.029200747088260925 1106 26-27 -0.06849366696597059 1106 28-29 -0.10063341523515845 1106 30-31 -0.12158239228318024 1106 32-33 -0.15900679743640467 1106 34-35 -0.27780480102251914 1106 36-37 -0.21131726185416966 1106 38-39 -0.12727949251068793 1106 40-41 0.004233314456094206 1106 42-43 -0.2238132391130634 1106 44-45 -0.09587206484716404 1106 46-47 -0.04352321354086541 1106 48-49 -0.22381790542530666 1106 50-51 -0.1078007105884069 1106 52-53 -0.011690900821250239 1106 54-55 -0.23879905538537827 1106 56-57 -0.13680855267004688 1106 58-59 -0.2046710021783653 1106 60-61 -0.16432179991669926 1106 62-63 -0.4920289304964527 1106 64-65 -0.20378710037543613 1106 66-67 -0.1601673008534661 1106 68-69 -0.2111888051212958 1106 70-71 -0.18554709068980202 1106 72-73 -0.12184414416229217 1106 74-75 -0.13449988796909196 1106 76-77 -0.13657788102724666 1106 78-79 -0.12037292846470393 1106 80-81 -0.2122073175109911 1106 82-83 -0.13238821004687296 1106 84-85 -0.05293108890637299 1106 86-87 -0.15341742765872368 1106 88-89 -0.2308885067605786 1106 90-91 -0.2793317232422794 1106 92-93 -0.003166865047663947 1106 94-95 -0.08953602235959224 1106 96-97 -0.1014583961457447 1106 98-99 -0.13956176295516087 1106 100-101 -0.07928971199377699 1107 1 0.5625015395223762 1107 2 0.6236515176835802 1107 3 0.396112616693582 1107 4 0.15401858638220745 1107 5 0.09327064239801075 1107 6 0.1467587094827678 1107 7 0.20911142836644103 1107 8 0.17199349710068645 1107 9 0.24752407793345554 1107 10-11 0.3092147631316706 1107 12-13 0.3469711181255022 1107 14-15 0.46947352936756204 1107 16-17 0.4068275900404501 1107 18-19 0.4353775605677157 1107 20-21 0.33202317539943493 1107 22-23 0.3077267500041856 1107 24-25 0.32263697307245565 1107 26-27 0.3913927699974735 1107 28-29 0.39536667694745375 1107 30-31 0.41417070995923666 1107 32-33 0.41346769622827395 1107 34-35 0.4049000302386929 1107 36-37 0.4989639248631974 1107 38-39 0.5270754670627653 1107 40-41 0.7449544650140965 1107 42-43 0.5128585669822883 1107 44-45 0.32982800265430257 1107 46-47 0.5410387257434977 1107 48-49 0.7212784254091957 1107 50-51 0.7034761970713745 1107 52-53 0.3796770892111354 1107 54-55 0.5817920136058774 1107 56-57 0.593104557779597 1107 58-59 0.47643066930063327 1107 60-61 0.5804557574818503 1107 62-63 0.4475044767812193 1107 64-65 0.4439563583758144 1107 66-67 0.46810220840791317 1107 68-69 0.38728750543302226 1107 70-71 0.2736219573611294 1107 72-73 0.45593084996477984 1107 74-75 0.790228173311533 1107 76-77 0.6169205979595738 1107 78-79 0.5316934489666103 1107 80-81 0.5784244010406105 1107 82-83 0.48122351974506117 1107 84-85 0.5082071731242017 1107 86-87 0.9938220003332745 1107 88-89 1.0911239587081099 1107 90-91 0.9762312003145404 1107 92-93 1.0496768199970425 1107 94-95 0.8389841641466553 1107 96-97 1.0727135958016554 1107 98-99 1.255776770270728 1107 100-101 1.2084847171430653 1108 1 0.2897583019284049 1108 2 0.23934057379081963 1108 3 0.007099513354010156 1108 4 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-1.5392177516574677 2316 52-53 -1.6461067610827484 2316 54-55 -1.2897455014426384 2316 56-57 -1.1372261553427023 2316 58-59 -1.4393428240780253 2316 60-61 -1.2869310246157326 2316 62-63 -1.2791898083260733 2316 64-65 -1.3044153043959383 2316 66-67 -1.3315928379769026 2316 68-69 -1.2480202808427414 2316 70-71 -1.1610644140006734 2316 72-73 -1.197503308380945 2316 74-75 -1.2857220957912219 2316 76-77 -1.3353902334263026 2316 78-79 -1.1997955567695264 2316 80-81 -1.1580438908650628 2316 82-83 -1.1556709990567242 2316 84-85 -1.2769151624044461 2316 86-87 -1.3696729716942073 2316 88-89 -1.201455798391244 2316 90-91 -1.2994101317440965 2316 92-93 -1.4513013331082618 2316 94-95 -1.6109892796604406 2316 96-97 -1.734979290679469 2316 98-99 -2.1479955369313117 2316 100-101 -2.6479741915744484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 11.0 4 30.0 5 55.0 6 191.0 7 408.0 8 984.0 9 1720.0 10 2946.0 11 4567.0 12 6211.0 13 8547.0 14 10971.0 15 14316.0 16 17517.0 17 21824.0 18 19483.0 19 14317.0 20 13644.0 21 14176.0 22 15225.0 23 16824.0 24 20443.0 25 24389.0 26 30493.0 27 37675.0 28 46523.0 29 57741.0 30 70888.0 31 87483.0 32 110066.0 33 138787.0 34 182216.0 35 247407.0 36 349038.0 37 445130.0 38 265272.0 39 15916.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.052546234815544 18.971345247913668 14.946898108671036 23.029210408599745 2 16.0129882593813 20.189947457294462 39.13454546712168 24.662518816202557 3 20.153839104389093 23.328488937573 29.319862397258316 27.19780956077959 4 13.542371870193268 16.537838681423956 35.910844117273236 34.00894533110954 5 14.162014867889491 37.09148532792263 33.86400004841271 14.88249975577516 6 33.330422807228366 35.54353210497605 16.50289914335436 14.623145944441227 7 29.62555361232311 32.11401020643699 19.65435053050822 18.60608565073168 8 27.006728502378706 34.59144426356918 18.811148064226376 19.59067916982574 9 26.212524142414743 16.70011014301301 19.670383633128665 37.416982081443585 10-11 25.71564547279458 26.144704240791615 26.41099645721775 21.728653829196055 12-13 26.63924425848732 23.233488362442625 27.126268648393854 23.0009987306762 14-15 23.17728803602771 25.057603574587905 25.485840035289414 26.27926835409497 16-17 22.762273702993202 28.002258107184012 26.24364475098144 22.991823438841347 18-19 22.61027564345154 26.633977993392094 28.609951883373753 22.14579447978261 20-21 23.458789976148093 25.607039208244686 28.69758567537029 22.23658514023693 22-23 22.944631253250062 25.54150419055449 28.71157685795514 22.80228769824031 24-25 23.033492951195665 25.67289346350589 28.88455816052445 22.409055424773992 26-27 22.768589566669398 25.79903144298224 28.899153058333223 22.53322593201514 28-29 22.543530375950642 26.346370473087287 28.69136118389828 22.418737967063795 30-31 22.798756139120844 25.87966328959187 28.510894156845083 22.810686414442202 32-33 22.706448043873838 25.832787410349578 28.62699090781571 22.83377363796088 34-35 23.2168518642993 25.8785927991322 28.465596206843564 22.43895912972494 36-37 23.278738695940383 25.70665434416732 28.534395615772308 22.480211344119986 38-39 22.888161593090466 25.95897754880664 28.598185256979807 22.55467560112309 40-41 23.05406920109045 25.904577304132797 28.440888672945125 22.600464821831626 42-43 22.95399918728007 25.999148373263242 28.018325105264523 23.028527334192166 44-45 23.034655932677413 25.985271023286515 28.061199127344654 22.918873916691414 46-47 23.468406783412178 26.43277796220986 27.551689974271724 22.547125280106233 48-49 23.568203953690748 26.445499822542583 27.27650200088102 22.70979422288565 50-51 23.28718538584932 26.651606770728648 27.38000604297776 22.681201800444274 52-53 23.615096644486485 26.667225728002684 27.03720041678887 22.68047721072196 54-55 23.292674597252855 26.59278898237017 27.128596365786834 22.985940054590145 56-57 23.37237392426562 26.780509660941448 26.9166103092837 22.930506105509235 58-59 23.64119945414125 26.477542546335098 26.884861756560312 22.99639624296334 60-61 23.354520747859247 26.614873928111205 27.404646908365155 22.625958415664392 62-63 23.140318851267438 26.66206875087265 27.40732447553717 22.790287922322744 64-65 23.142950648443332 26.76699618430507 27.162832381787382 22.92722078546422 66-67 23.1671159379193 26.70441380908248 26.98608008382554 23.14239016917267 68-69 23.172932262576023 26.625668565930265 27.06445227357263 23.136946897921085 70-71 23.370656207098076 26.533057608316014 26.957538116793522 23.13874806779239 72-73 23.316058928643983 26.58337663101128 26.996246285837294 23.10431815450744 74-75 23.166243883841453 27.14886025862335 26.56014859247576 23.124747265059437 76-77 23.361696085214042 26.77229376488461 26.750248495572464 23.11576165432888 78-79 23.296241402393594 26.864022455312764 26.751560003138486 23.088176139155156 80-81 23.49712412674896 26.839509433888853 26.58442145436679 23.0789449849954 82-83 23.46788131775781 26.9565952303344 26.630488820258673 22.945034631649115 84-85 23.39818720408598 27.088903082315024 26.510049053777596 23.0028606598214 86-87 23.3668080485432 27.04448763065287 26.376679274532222 23.21202504627171 88-89 23.31078802364555 27.074736339643657 26.146909578938992 23.467566057771798 90-91 23.281375146324933 26.96758531162482 26.43918341229205 23.311856129758198 92-93 23.401556416533133 26.915229634765588 26.554676662959263 23.128537285742013 94-95 23.083929457617682 26.82799199057959 26.716332162699135 23.371746389103592 96-97 23.146643922193842 26.759634555229123 26.765708297813106 23.328013224763925 98-99 22.990056790549346 26.943780511897398 26.287838211130115 23.778324486423138 100-101 22.72034511402246 27.07607383949509 26.389343104474243 23.814237942008205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 145.0 2 147.5 3 179.5 4 174.0 5 163.0 6 278.0 7 624.0 8 1049.5 9 1339.5 10 1350.5 11 1291.0 12 1382.5 13 1513.0 14 1736.0 15 1980.5 16 2254.0 17 2503.5 18 2690.0 19 3055.5 20 3587.5 21 4153.5 22 4868.5 23 5906.5 24 7200.5 25 8903.0 26 11216.5 27 14265.0 28 17384.0 29 20774.5 30 24827.5 31 28799.5 32 34084.5 33 37680.0 34 39526.5 35 43254.0 36 48451.5 37 52533.0 38 54902.0 39 57635.5 40 60341.0 41 63013.5 42 65156.5 43 68479.0 44 72506.5 45 76267.0 46 80032.5 47 85717.5 48 95751.5 49 96774.5 50 88751.5 51 85065.0 52 85054.0 53 85416.0 54 85763.0 55 81999.0 56 73933.0 57 67115.0 58 63790.0 59 58531.0 60 49116.0 61 40462.5 62 33332.0 63 26391.0 64 19122.0 65 13928.0 66 10665.0 67 8426.0 68 6735.0 69 5353.5 70 4161.5 71 3014.0 72 2335.5 73 1992.0 74 1532.0 75 1122.5 76 825.0 77 509.5 78 328.0 79 245.5 80 164.5 81 125.0 82 87.0 83 52.5 84 33.0 85 13.5 86 6.5 87 5.5 88 5.5 89 2.0 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.048567459223097 2 0.011930275321360985 3 0.0050141736857893995 4 0.012103177862250274 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0037174046291197273 10-11 2.593538113339345E-4 12-13 2.3774099372277327E-4 14-15 2.1612817611161204E-5 16-17 0.0 18-19 2.1612817611161204E-5 20-21 0.0 22-23 0.0010806408805580602 24-25 0.0 26-27 2.1612817611161204E-5 28-29 0.0 30-31 0.0 32-33 0.0035228892706192765 34-35 5.403204402790301E-4 36-37 0.0025286996605058606 38-39 6.483845283348362E-5 40-41 0.003566114905841599 42-43 0.009596091019355575 44-45 0.0018587023145598637 46-47 0.001469671597558962 48-49 0.009120609031910028 50-51 5.619332578901913E-4 52-53 0.0017506382265040576 54-55 0.00881802958535377 56-57 0.0034796636353969543 58-59 9.941896101134154E-4 60-61 0.005143850591456367 62-63 0.003241922641674181 64-65 0.015734131220925356 66-67 0.0028528919246732787 68-69 6.483845283348362E-5 70-71 0.0011238665157803827 72-73 0.0 74-75 2.1612817611161204E-5 76-77 8.861255220576094E-4 78-79 0.009747380742633703 80-81 0.013767364818309688 82-83 0.010374152453357379 84-85 0.007110616994072037 86-87 0.00879641676774261 88-89 0.02671344256739525 90-91 0.022671845674108106 92-93 0.0022045073963384426 94-95 0.00717545544690552 96-97 0.008904480855798418 98-99 0.008861255220576094 100-101 0.010179637094856928 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2313442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.04953638748118 #Duplication Level Percentage of deduplicated Percentage of total 1 77.48248832970029 44.20340037357822 2 13.109556876443904 14.957882840928812 3 4.110305809623068 7.034731225492993 4 1.7928757732628329 4.091309266599658 5 0.9210930223972917 2.627396494875466 6 0.5715155221150345 1.9562817344947179 7 0.36918079687780303 1.4743115313527666 8 0.26257128577769284 1.1983656097825768 9 0.18524773927407462 0.9511467878173452 >10 1.0659104598680664 11.381912327189351 >50 0.08627625336336059 3.359223203950333 >100 0.03895766588811012 3.9866797861172523 >500 0.0027308821643138442 1.0339199326326256 >1k 0.001137867568463654 1.1729241946395514 >5k 1.5171567579515388E-4 0.570514690548397 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6820 0.2947988322162388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6363 0.2750447169196375 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4965 0.21461527887883078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3628 0.1568226045865857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.645127044464482E-5 2 0.0 0.0 0.0 0.0 8.645127044464482E-5 3 0.0 0.0 0.0 0.0 8.645127044464482E-5 4 0.0 0.0 0.0 0.0 8.645127044464482E-5 5 4.322563522232241E-5 0.0 0.0 0.0 8.645127044464482E-5 6 4.322563522232241E-5 0.0 0.0 0.0 8.645127044464482E-5 7 1.2967690566696724E-4 0.0 0.0 0.0 8.645127044464482E-5 8 1.2967690566696724E-4 0.0 0.0 0.0 8.645127044464482E-5 9 1.2967690566696724E-4 0.0 0.0 0.0 8.645127044464482E-5 10-11 1.2967690566696724E-4 0.0 0.0 0.0 8.645127044464482E-5 12-13 1.2967690566696724E-4 0.0 0.0 0.0 1.5128972327812845E-4 14-15 1.2967690566696724E-4 0.0 0.0 0.0 2.3774099372277327E-4 16-17 1.5128972327812845E-4 0.0 0.0 4.322563522232241E-5 2.593538113339345E-4 18-19 1.9451535850045085E-4 0.0 0.0 8.645127044464482E-5 3.4580508177857927E-4 20-21 2.1612817611161206E-4 0.0 0.0 1.2967690566696724E-4 3.674178993897405E-4 22-23 2.1612817611161206E-4 0.0 0.0 3.241922641674181E-4 4.5386916983438535E-4 24-25 2.593538113339345E-4 0.0 0.0 3.890307170009017E-4 5.18707622667869E-4 26-27 2.593538113339345E-4 0.0 0.0 7.34835798779481E-4 5.18707622667869E-4 28-29 3.025794465562569E-4 0.0 0.0 0.001383220327114317 5.18707622667869E-4 30-31 3.4580508177857927E-4 0.0 0.0 0.0028961175598956014 5.619332578901913E-4 32-33 3.4580508177857927E-4 0.0 0.0 0.005619332578901914 6.483845283348362E-4 34-35 4.106435346120629E-4 0.0 0.0 0.007910291245685002 7.780614340018034E-4 36-37 4.322563522232241E-4 0.0 0.0 0.00931512439041048 7.780614340018034E-4 38-39 5.619332578901913E-4 0.0 0.0 0.010698344717524795 8.212870692241258E-4 40-41 5.619332578901913E-4 0.0 0.0 0.0125354342144735 8.212870692241258E-4 42-43 5.835460755013525E-4 0.0 0.0 0.015042521057368198 8.645127044464482E-4 44-45 7.780614340018034E-4 0.0 0.0 0.017527995082651737 9.077383396687706E-4 46-47 9.077383396687706E-4 0.0 0.0 0.021137335623715656 9.077383396687706E-4 48-49 9.077383396687706E-4 0.0 0.0 0.025157319699391643 0.001037415245335738 50-51 9.077383396687706E-4 0.0 0.0 0.030214719020403364 0.0011238665157803827 52-53 9.077383396687706E-4 0.0 0.0 0.04171273798954113 0.0012319306038361885 54-55 9.077383396687706E-4 0.0 0.0 0.056214938606630294 0.0012751562390585112 56-57 9.077383396687706E-4 0.0 0.0 0.07363486960122623 0.0013399946918919946 58-59 9.077383396687706E-4 0.0 0.0 0.10054282752712193 0.0013399946918919946 60-61 9.077383396687706E-4 0.0 0.0 0.136484943214483 0.0013399946918919946 62-63 9.077383396687706E-4 0.0 0.0 0.16045355794526078 0.0014264459623366396 64-65 9.077383396687706E-4 0.0 0.0 0.187296677418323 0.0014264459623366396 66-67 9.941896101134154E-4 0.0 0.0 0.24286323149661845 0.0014264459623366396 68-69 0.001037415245335738 0.0 0.0 0.31200263503472314 0.0014264459623366396 70-71 0.0011022536981692215 0.0 0.0 0.36303049741467475 0.0014480587799478007 72-73 0.0011887049686138663 0.0 0.0 0.40588871473760746 0.0015561228680036068 74-75 0.0012103177862250274 0.0 0.0 0.5051347732080598 0.0016425741384482515 76-77 0.0012103177862250274 0.0 0.0 0.5732583743184398 0.0017290254088928965 78-79 0.0012103177862250274 0.0 0.0 0.6254533288493941 0.0017290254088928965 80-81 0.0014264459623366396 0.0 0.0 0.6990233599977869 0.0017290254088928965 82-83 0.0014264459623366396 0.0 0.0 0.8327634753756523 0.0017722510441152187 84-85 0.0014480587799478007 0.0 0.0 0.971733892615419 0.0017722510441152187 86-87 0.0014912844151701232 0.0 0.0 1.1627695874804729 0.0017722510441152187 88-89 0.0015128972327812843 0.0 0.0 1.4242630677579122 0.00179386386172638 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 975 0.0 22.893362 4 GTATCAA 15235 0.0 22.306501 1 GGTATCA 11730 0.0 21.687948 1 GAGTACT 8675 0.0 21.649897 12-13 GTACATG 8680 0.0 21.179695 1 GTACTTT 8920 0.0 20.868921 14-15 TAAGGTG 1125 0.0 20.68476 5 ATACCGT 300 1.6734703E-10 20.579227 6 TACATGG 8660 0.0 20.347445 2 AGTACTT 9045 0.0 20.213009 12-13 GGTTGCG 310 2.6011548E-10 19.91538 7 ACATGGG 8625 0.0 19.823845 3 AGAGTAC 13665 0.0 19.304262 10-11 TCAACGC 17705 0.0 19.232668 4 ATCAACG 17745 0.0 19.190561 3 CAACGCA 17790 0.0 19.140362 5 TACCGTC 225 1.6723152E-6 18.996208 7 AACGCAG 18040 0.0 18.954088 6 TATCAAC 18015 0.0 18.85062 2 TACTTTT 10090 0.0 18.001928 14-15 >>END_MODULE