FastQCFastQC Report
Thu 26 May 2016
SRR936657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380303
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63120.2651763241906598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.21669510142196183No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40940.17199490989172386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.1225894350425135No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28350.11910248401148929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA1551.4069683E-624.525587
GTACATG93650.024.4060941
TACATGG91900.023.9923382
GTATCAA133300.022.992731
GGTATCA102600.022.508671
ACATGGG93250.022.4729373
GAGTACT73150.021.9871412-13
GTACTTT76400.020.89590814-15
TAGCCCT16450.019.6436524
TCAACGC154500.019.5617564
ATCAACG154950.019.5352043
CAACGCA155700.019.3796565
TATCAAC158300.019.1518122
AACGCAG158150.019.0790336
AGTACTT77800.018.87136512-13
ACCGTAT2051.5855754E-518.543348
GTGTAGC14700.018.4254651
AGAGTAC124800.018.35165210-11
CATGGGG54900.018.0906074
GTACCGT2956.3202606E-817.7183196
Produced by FastQC (version 0.11.5)