##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936657_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2380303 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.235641848957886 31.0 31.0 34.0 28.0 34.0 2 31.28020634347812 31.0 31.0 34.0 28.0 34.0 3 31.328054453571667 31.0 31.0 34.0 28.0 34.0 4 34.94920016485296 37.0 35.0 37.0 32.0 37.0 5 34.91066179389767 37.0 35.0 37.0 32.0 37.0 6 35.02860392143353 37.0 35.0 37.0 32.0 37.0 7 34.881578941840594 37.0 35.0 37.0 32.0 37.0 8 34.82745642046412 37.0 35.0 37.0 32.0 37.0 9 36.28948247344981 39.0 37.0 39.0 32.0 39.0 10-11 36.19031631687226 39.0 35.0 39.0 32.0 39.0 12-13 36.076751783281374 39.0 35.0 39.0 31.0 39.0 14-15 37.10948438077001 39.0 36.0 41.0 31.0 41.0 16-17 36.976667676342046 39.0 36.0 41.0 31.0 41.0 18-19 36.91123609053133 39.0 36.0 41.0 31.0 41.0 20-21 36.80357836796408 39.0 36.0 41.0 30.0 41.0 22-23 36.66056590274431 39.0 36.0 40.5 30.0 41.0 24-25 36.79917703754522 39.0 36.0 41.0 30.0 41.0 26-27 36.77736384821596 39.0 36.0 41.0 30.0 41.0 28-29 36.677119047449 39.0 36.0 41.0 30.0 41.0 30-31 36.491783609061535 39.0 36.0 41.0 30.0 41.0 32-33 36.30936649661828 39.0 35.0 40.5 30.0 41.0 34-35 36.08977785601245 39.0 35.0 40.0 29.0 41.0 36-37 35.89246957215111 38.0 35.0 40.0 28.5 41.0 38-39 35.67723016775595 38.0 35.0 40.0 27.0 41.0 40-41 35.48842185217596 38.0 34.5 40.0 27.0 41.0 42-43 35.244810219539275 38.0 34.0 40.0 26.0 41.0 44-45 34.98554847849202 38.0 34.0 40.0 25.5 41.0 46-47 34.72490519064169 38.0 34.0 40.0 24.0 41.0 48-49 34.63380880501348 38.0 34.0 40.0 24.0 41.0 50-51 33.71135376462577 37.5 32.0 39.5 23.5 40.5 52-53 33.862292951779665 37.5 33.0 39.5 23.0 40.5 54-55 34.2713102071459 38.0 33.0 40.0 23.0 41.0 56-57 34.15740117959773 38.0 33.0 40.0 23.0 41.0 58-59 33.882770806909875 37.5 33.0 40.0 22.5 41.0 60-61 33.54140271217572 37.0 33.0 40.0 22.0 41.0 62-63 33.19701105279454 36.5 32.0 39.5 20.5 41.0 64-65 32.84107569498505 36.0 32.0 39.0 20.0 41.0 66-67 32.437841736955335 35.5 31.5 39.0 18.5 40.5 68-69 32.012698383357076 35.0 31.0 38.5 17.5 40.0 70-71 31.570895806122163 35.0 31.0 38.0 14.0 40.0 72-73 31.171852280991118 35.0 30.5 37.0 12.0 39.5 74-75 30.74218933471915 35.0 30.0 37.0 8.0 39.0 76-77 30.309775898278495 34.0 30.0 36.0 7.0 39.0 78-79 29.868382092531917 34.0 29.0 36.0 4.5 37.5 80-81 29.397045040064228 34.0 29.0 35.0 2.0 37.0 82-83 28.917496007861185 34.0 29.0 35.0 2.0 37.0 84-85 28.591599682897513 34.0 28.0 35.0 2.0 36.0 86-87 28.26526286779456 34.0 27.5 35.0 2.0 36.0 88-89 27.852692283293344 33.5 27.0 35.0 2.0 35.5 90-91 27.429448897892414 33.0 26.0 35.0 2.0 35.0 92-93 27.04175560842464 33.0 25.5 35.0 2.0 35.0 94-95 26.557493520782856 33.0 25.0 35.0 2.0 35.0 96-97 25.950626873973608 33.0 23.5 35.0 2.0 35.0 98-99 25.201100868250805 32.0 19.0 35.0 2.0 35.0 100-101 22.68887553391312 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2502267345791971 1101 2 0.2934161212777475 1101 3 0.299314256407623 1101 4 0.4265147738321673 1101 5 0.3035674943493021 1101 6 0.18226440004065125 1101 7 0.1904990685531729 1101 8 0.2135524158837967 1101 9 0.44645883753516813 1101 10-11 0.2829406545187041 1101 12-13 0.4565067962959901 1101 14-15 0.6998264617556629 1101 16-17 0.5696095777530346 1101 18-19 0.515714163532877 1101 20-21 0.5560969199310506 1101 22-23 0.5477176976164202 1101 24-25 0.5606090866032005 1101 26-27 0.5028862596366892 1101 28-29 0.5316555496770974 1101 30-31 0.5156097625979683 1101 32-33 0.48142482037086864 1101 34-35 0.4114180049753102 1101 36-37 0.5035088476948317 1101 38-39 0.2733474171977548 1101 40-41 0.34493580190089546 1101 42-43 0.4068731480967287 1101 44-45 0.27799416478364236 1101 46-47 0.427104361408837 1101 48-49 0.6774284587742869 1101 50-51 0.5467557430930015 1101 52-53 0.640913531209307 1101 54-55 0.8407522292868919 1101 56-57 0.6309653949682499 1101 58-59 0.5194880626527691 1101 60-61 0.48254841572497753 1101 62-63 0.5338851734444603 1101 64-65 0.6318884545585348 1101 66-67 0.4771089753739588 1101 68-69 0.4443136505953511 1101 70-71 0.5129797426947356 1101 72-73 0.305180855967361 1101 74-75 0.1598355879758202 1101 76-77 0.4092691294105464 1101 78-79 0.30002526747864167 1101 80-81 0.3772028110670078 1101 82-83 0.5727469330261563 1101 84-85 0.44240684676708497 1101 86-87 0.4321111354193725 1101 88-89 0.48413298629501966 1101 90-91 0.3661525922562987 1101 92-93 0.26895147169021527 1101 94-95 0.3463896247710494 1101 96-97 0.23567567048268145 1101 98-99 0.32559855088839385 1101 100-101 0.823229600915365 1102 1 0.2910564049600701 1102 2 0.4624105972650199 1102 3 0.38560645067752475 1102 4 0.4164043740002654 1102 5 0.4184541424966426 1102 6 0.26862744280876427 1102 7 0.35785343372062783 1102 8 0.37619414319826916 1102 9 0.5359804467739124 1102 10-11 0.4041516481120837 1102 12-13 0.3233663198085637 1102 14-15 0.5059708647817516 1102 16-17 0.4313989018928268 1102 18-19 0.5920533565263639 1102 20-21 0.5376097383391993 1102 22-23 0.4385386300158487 1102 24-25 0.3109867218641469 1102 26-27 0.22533203404715607 1102 28-29 0.3033544979407168 1102 30-31 0.15585940717466684 1102 32-33 0.18814184521392718 1102 34-35 -0.06895306754457664 1102 36-37 0.11090521389450458 1102 38-39 0.0629241861411387 1102 40-41 0.16688522832804864 1102 42-43 0.23598414497084974 1102 44-45 -0.029411086200674674 1102 46-47 0.14237286381327863 1102 48-49 0.21786648376325957 1102 50-51 0.22331128569867076 1102 52-53 0.21846710575858452 1102 54-55 0.3703564976599836 1102 56-57 0.4519325165418522 1102 58-59 0.42546087189274573 1102 60-61 0.2571232385686315 1102 62-63 0.28303623610172224 1102 64-65 0.24916180351088002 1102 66-67 0.4265816774476008 1102 68-69 0.25819413517865186 1102 70-71 0.15265181244233617 1102 72-73 0.1392186318759876 1102 74-75 -0.023680468527832232 1102 76-77 0.19557461256092523 1102 78-79 0.03216356323652647 1102 80-81 0.18202336841618916 1102 82-83 0.3483938021544226 1102 84-85 0.33931915432565063 1102 86-87 0.19806644061236156 1102 88-89 0.3199653355150538 1102 90-91 0.2736623671150902 1102 92-93 0.16731240764100264 1102 94-95 0.2687678186500726 1102 96-97 0.2130285331513555 1102 98-99 0.39515320233484275 1102 100-101 0.46293581836255626 1103 1 0.18766964112269235 1103 2 0.31073917555638886 1103 3 0.29682670205885486 1103 4 0.2730290459487108 1103 5 0.48252852607895136 1103 6 0.41430061206612123 1103 7 0.24303371597498113 1103 8 0.3542643118619466 1103 9 0.48727563576301947 1103 10-11 0.5197962896921027 1103 12-13 0.4634691775192721 1103 14-15 0.3259713513561593 1103 16-17 0.48910360168671474 1103 18-19 0.37189755231154464 1103 20-21 0.43891140211474067 1103 22-23 0.5679657491503747 1103 24-25 0.5873343398893383 1103 26-27 0.42355163511793137 1103 28-29 0.2519804787485782 1103 30-31 0.27724697836514167 1103 32-33 0.5271272294455187 1103 34-35 0.4204324018504124 1103 36-37 0.524816427709716 1103 38-39 0.4343666578567493 1103 40-41 0.2631687137866763 1103 42-43 0.509131763922845 1103 44-45 0.5163285171974152 1103 46-47 0.32350971273681495 1103 48-49 0.2984689504123139 1103 50-51 0.3075862306179289 1103 52-53 0.3475208627051387 1103 54-55 0.34629912616068736 1103 56-57 0.4027597960142302 1103 58-59 0.24609924721822551 1103 60-61 0.34302402424469847 1103 62-63 0.3273580157386107 1103 64-65 0.2739350701346055 1103 66-67 0.1841155999221442 1103 68-69 0.2865669582730668 1103 70-71 0.35219971015770213 1103 72-73 0.35475281591645214 1103 74-75 0.2560607485466555 1103 76-77 0.22540574292218452 1103 78-79 0.3424108717591956 1103 80-81 0.23919180657412653 1103 82-83 0.1504993563626762 1103 84-85 0.2715771839618917 1103 86-87 0.29413624524629967 1103 88-89 0.24116306545235489 1103 90-91 0.32846808747949297 1103 92-93 0.38652511995092453 1103 94-95 0.5569424128753333 1103 96-97 0.751580351266238 1103 98-99 0.8390108183056064 1103 100-101 0.9853550560214579 1104 1 0.09496165153298364 1104 2 0.2514876529389909 1104 3 0.19100759637431253 1104 4 0.2380072439258143 1104 5 0.31949019018789926 1104 6 0.1944114857382644 1104 7 0.3833510654299559 1104 8 0.24706916373314414 1104 9 0.477152085626912 1104 10-11 0.4576841526453208 1104 12-13 0.22606033966373928 1104 14-15 0.35117223112624885 1104 16-17 0.2691953859070324 1104 18-19 0.4798459995782025 1104 20-21 0.5339644574415772 1104 22-23 0.30602536377305967 1104 24-25 0.42803459971947433 1104 26-27 0.009951562070739328 1104 28-29 0.2804654116978753 1104 30-31 0.24910298569549383 1104 32-33 -0.07765904344980612 1104 34-35 -0.19492956085493773 1104 36-37 -0.0780105732653098 1104 38-39 0.07142932251996115 1104 40-41 0.1875014640240309 1104 42-43 0.17976543226938446 1104 44-45 0.20065399475409862 1104 46-47 0.1864824236312046 1104 48-49 0.017485034677157785 1104 50-51 0.21140689763274167 1104 52-53 0.2656713004009106 1104 54-55 0.23160088901148868 1104 56-57 0.22305917201608594 1104 58-59 0.20625221913883252 1104 60-61 0.3012254668198935 1104 62-63 0.5354458560514743 1104 64-65 0.26716365443949996 1104 66-67 0.28244469886797674 1104 68-69 0.29963669164014917 1104 70-71 0.37443371533294467 1104 72-73 0.29803658609119665 1104 74-75 0.24439495759190422 1104 76-77 0.36408353540196003 1104 78-79 0.18660703763612219 1104 80-81 0.3013640153224877 1104 82-83 0.28326983120144433 1104 84-85 0.37495978143023123 1104 86-87 0.5273219089878758 1104 88-89 0.6436490727506659 1104 90-91 0.6539836895688111 1104 92-93 0.41950902287078407 1104 94-95 0.2746083522739333 1104 96-97 0.4927376633005487 1104 98-99 0.6882905185558279 1104 100-101 0.9653941640341124 1105 1 0.3774337234055203 1105 2 0.39113846058967283 1105 3 0.4243007946043349 1105 4 0.4121251309510541 1105 5 0.5001919480432733 1105 6 0.32184985570688696 1105 7 0.38832844917681797 1105 8 0.46212810498568757 1105 9 0.5703414731221059 1105 10-11 0.49951991268336116 1105 12-13 0.29398777780770047 1105 14-15 0.1986450191589455 1105 16-17 0.281767528096033 1105 18-19 0.35792841930117447 1105 20-21 0.2705669653864362 1105 22-23 0.19395074807702883 1105 24-25 0.4112935437406051 1105 26-27 0.38553497834399053 1105 28-29 0.12738984987941393 1105 30-31 0.15125721523569524 1105 32-33 0.19640026534687394 1105 34-35 0.19201353986712633 1105 36-37 0.1837713914204997 1105 38-39 0.13393357781085768 1105 40-41 0.02521745270220066 1105 42-43 0.21914251700878395 1105 44-45 -0.04337920456641342 1105 46-47 0.03868815455104624 1105 48-49 0.15875730238488472 1105 50-51 0.2087674166129716 1105 52-53 0.03976352148901441 1105 54-55 0.07318195311530928 1105 56-57 0.3698118306370901 1105 58-59 0.35813283877134694 1105 60-61 0.06652713975307734 1105 62-63 0.08509185822509835 1105 64-65 0.2637609048929903 1105 66-67 0.27233476333933737 1105 68-69 0.22808763014897337 1105 70-71 0.3476419094482246 1105 72-73 0.216448569442381 1105 74-75 0.37372373612777565 1105 76-77 0.20800603359460723 1105 78-79 0.41824296780104575 1105 80-81 0.13911475260019301 1105 82-83 0.33377515494115073 1105 84-85 0.4884876937371949 1105 86-87 0.5080855928027397 1105 88-89 0.43930611932876573 1105 90-91 0.5486554579702414 1105 92-93 0.6372957998138737 1105 94-95 0.4963910769087967 1105 96-97 0.7087358055217656 1105 98-99 0.6622177685488317 1105 100-101 1.2507062982733217 1106 1 0.15291645539272025 1106 2 0.1530376680272525 1106 3 0.23689922689350595 1106 4 0.09344538276357639 1106 5 0.1265303306875083 1106 6 0.23628448976132432 1106 7 0.29235911584048324 1106 8 0.28727588008381133 1106 9 0.44015151785057327 1106 10-11 0.39424894851042325 1106 12-13 0.4860316791153352 1106 14-15 0.6109733091861997 1106 16-17 0.5805093662193457 1106 18-19 0.5119196417418124 1106 20-21 0.3276315345661942 1106 22-23 0.3132686595073011 1106 24-25 0.29401607333503677 1106 26-27 0.044866445676589706 1106 28-29 0.3125233935318903 1106 30-31 0.32724953205649854 1106 32-33 0.11556475338637284 1106 34-35 0.13207978814730126 1106 36-37 0.1448931235735884 1106 38-39 0.29496043365967495 1106 40-41 0.06967743464267784 1106 42-43 -0.13115761186985964 1106 44-45 0.3525634823395265 1106 46-47 0.2710879620962672 1106 48-49 0.20250803891474334 1106 50-51 0.034153133075434994 1106 52-53 0.29038265692869203 1106 54-55 0.29753724085547617 1106 56-57 0.31762611027148324 1106 58-59 0.2519293245950678 1106 60-61 0.4002179856158605 1106 62-63 0.6280364464101496 1106 64-65 0.5210381200933227 1106 66-67 0.45910687480999 1106 68-69 0.46446773885139336 1106 70-71 0.46894809494791545 1106 72-73 0.6044365838029329 1106 74-75 0.2996001222112845 1106 76-77 0.4120090780431056 1106 78-79 0.5905326317632849 1106 80-81 0.6617968696928642 1106 82-83 0.6916674147911337 1106 84-85 0.5496426776329955 1106 86-87 0.6293663328379147 1106 88-89 0.5882544109923309 1106 90-91 0.8576646536158421 1106 92-93 0.9871060847355189 1106 94-95 0.7446421328205126 1106 96-97 0.9462773983477923 1106 98-99 1.3580339036755085 1106 100-101 1.5730096607525965 1107 1 0.21380656298414635 1107 2 0.20803419684714441 1107 3 0.03450047469938511 1107 4 0.1094382766970412 1107 5 0.1685549533293056 1107 6 0.23065284108719908 1107 7 0.2508256121313224 1107 8 0.24434526427523906 1107 9 0.3715002153243461 1107 10-11 0.4142202847480263 1107 12-13 0.3756340546757926 1107 14-15 0.3011194019106682 1107 16-17 0.3185132557600312 1107 18-19 0.36300942555853766 1107 20-21 0.4447111807180377 1107 22-23 0.6173841300568341 1107 24-25 0.26618008886894273 1107 26-27 0.2243718555638452 1107 28-29 0.24078274070750183 1107 30-31 0.22290631268095495 1107 32-33 0.13708872271872252 1107 34-35 0.22279877716989205 1107 36-37 0.36873118771614344 1107 38-39 0.2907284739435525 1107 40-41 0.12450176867630347 1107 42-43 0.2453988865127812 1107 44-45 0.25340861616944466 1107 46-47 0.23649916809640104 1107 48-49 0.2937266353453367 1107 50-51 0.21078261718834312 1107 52-53 0.3537513235407914 1107 54-55 0.36152495985055566 1107 56-57 0.43545372752613076 1107 58-59 0.520016825618228 1107 60-61 0.5541270801077474 1107 62-63 0.5098928608175157 1107 64-65 0.5821889451428035 1107 66-67 0.5454534653229004 1107 68-69 0.5085828001351551 1107 70-71 0.6773860184243112 1107 72-73 0.5416239123281521 1107 74-75 0.612045363966093 1107 76-77 0.5276162010865875 1107 78-79 0.46827789564876454 1107 80-81 0.530541545448262 1107 82-83 0.7500166640158028 1107 84-85 0.6967590212828583 1107 86-87 0.5411475029742441 1107 88-89 0.41686868254472387 1107 90-91 0.5286408131264189 1107 92-93 0.6350915876322141 1107 94-95 0.5454178430482166 1107 96-97 0.6647446828542023 1107 98-99 0.7993886188720047 1107 100-101 1.193050572172325 1108 1 -0.012157067105917463 1108 2 0.19525559673612847 1108 3 0.14014098616843285 1108 4 0.25603744258443584 1108 5 0.4022696298106325 1108 6 0.12267138859110815 1108 7 0.012642832456066344 1108 8 0.18783576049222717 1108 9 0.19950949657731343 1108 10-11 0.12755721023297184 1108 12-13 0.2751673666729175 1108 14-15 0.3675596213402912 1108 16-17 0.3668413425517443 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2316 84-85 -2.4564352341603772 2316 86-87 -2.7506772412666187 2316 88-89 -3.1275159312043037 2316 90-91 -2.9303238179972197 2316 92-93 -3.451989538436994 2316 94-95 -3.621476764139768 2316 96-97 -4.70402194756473 2316 98-99 -6.091774781448759 2316 100-101 -6.146666741080981 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6499.0 3 2740.0 4 2698.0 5 2984.0 6 3238.0 7 3854.0 8 4706.0 9 5908.0 10 7468.0 11 8963.0 12 10687.0 13 12706.0 14 14325.0 15 16274.0 16 19179.0 17 22751.0 18 20216.0 19 16539.0 20 16962.0 21 18444.0 22 20848.0 23 23281.0 24 26820.0 25 30664.0 26 35943.0 27 41758.0 28 49551.0 29 59467.0 30 71169.0 31 86516.0 32 107579.0 33 135196.0 34 176125.0 35 238539.0 36 324991.0 37 404914.0 38 289607.0 39 40183.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5540281175418 17.886658225156346 14.210188563952848 23.349125093349013 2 15.827237158533322 19.040361446542544 40.232232429086665 24.90016896583747 3 19.969543646333157 23.073335185030334 28.927443031953537 28.02967813668297 4 12.989360789937809 15.71746575630358 36.533698958103905 34.7594744956547 5 14.32684195858517 36.62820097326485 33.926775207933744 15.118181860216234 6 33.373277487175066 34.786636431842396 17.14146409124248 14.698621989740055 7 29.7333876953987 31.784785066684602 19.85149908999584 18.63032814792086 8 26.83975497252372 34.07676546919819 19.315394071848107 19.768085486429975 9 26.323122469839817 16.69666153349562 19.929886823665488 37.05032917299908 10-11 25.655214881226346 25.733185378535207 26.818824726104985 21.79277501413347 12-13 26.546919778074646 22.960775549291725 27.275168817642903 23.217135854990726 14-15 23.29985143282221 24.709789673425387 25.617268255165705 26.3730906385867 16-17 22.8547717109834 27.713192549257126 26.256609892657153 23.175425847102325 18-19 22.68145298996211 26.510574954642134 28.42145813048479 22.38651392491096 20-21 23.565527234011174 25.52588108676804 28.359900581064228 22.548691098156556 22-23 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27.54622058797272 22.934570153520724 48-49 23.385021930193087 26.128480682944865 27.391609390491872 23.094887996370176 50-51 23.197242530136982 26.392976845756287 27.457864519484538 22.951916104622192 52-53 23.409409490575364 26.432779765101987 27.092045309171464 23.06576543515118 54-55 23.107995805302863 26.268513554619293 27.30265092758938 23.320839712488468 56-57 23.192515683366178 26.499972688042828 27.036022370593848 23.271489257997153 58-59 23.42869303511998 26.22160710676833 27.08995349239782 23.25974636571387 60-61 23.237037717072692 26.295230414325417 27.502537237145695 22.965194631456196 62-63 23.002149437138204 26.34806176732805 27.53201678207917 23.11777201345457 64-65 22.966912818853906 26.46905172766907 27.24573872438482 23.318296729092207 66-67 22.93328112359612 26.470840938792133 27.120150528739128 23.475727408872615 68-69 22.971125293761972 26.352124435323628 27.32495820207798 23.351792068836417 70-71 23.205819121711922 26.21736921954177 27.067440622514603 23.509371036231705 72-73 23.07906572250219 26.317023319126054 27.205422993948076 23.39848796442368 74-75 22.98954376217857 26.78673610603452 26.87794669873112 23.345773433055793 76-77 23.1591726365561 26.46108063436631 26.925024126742176 23.45472260233541 78-79 23.082253995943137 26.534642150708944 27.0118991800565 23.37120467329142 80-81 23.244157485502427 26.500028990938862 26.83023158298089 23.425581940577818 82-83 23.248051126049905 26.56545903599196 26.961651471237293 23.224838366720842 84-85 23.22219161856044 26.63667787937213 26.800214883926333 23.340915618141096 86-87 23.111525922975655 26.684677792223372 26.784732598645782 23.41906368615519 88-89 23.13267914432212 26.691638503151903 26.502238516260334 23.673443836265644 90-91 23.028777700800475 26.558422761679534 26.827432525939926 23.585367011580065 92-93 23.21889575192235 26.4651245850666 26.97317114164461 23.342808521366443 94-95 22.996497526083683 26.36535979348177 27.12953019119071 23.50861248924384 96-97 22.901864099354768 26.288183113403473 27.15464667739741 23.655306109844346 98-99 22.807688396558692 26.501768640478968 26.843352828017885 23.847190134944455 100-101 22.865563249983452 26.276656884901932 26.930280498145038 23.927499366969577 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 98.0 1 82.5 2 92.5 3 140.0 4 153.0 5 168.0 6 263.5 7 566.5 8 1010.0 9 1268.5 10 1263.5 11 1205.0 12 1280.0 13 1462.5 14 1645.0 15 1810.0 16 2020.0 17 2218.0 18 2448.0 19 2744.0 20 3173.0 21 3741.5 22 4444.0 23 5466.5 24 6782.0 25 8516.0 26 11029.0 27 14163.0 28 17045.5 29 20482.5 30 24582.0 31 28746.5 32 34173.5 33 38143.0 34 40687.5 35 44329.0 36 49101.0 37 53373.5 38 56135.0 39 58771.5 40 61343.0 41 64266.0 42 66823.0 43 70458.0 44 74911.0 45 79637.5 46 83493.0 47 88787.0 48 98162.0 49 99242.0 50 92102.0 51 88173.0 52 87967.5 53 88669.5 54 88637.5 55 84191.5 56 77282.5 57 70684.5 58 66018.5 59 60340.0 60 51131.5 61 42979.5 62 35889.5 63 28281.5 64 20706.0 65 15286.5 66 11397.5 67 8833.0 68 6904.5 69 5391.0 70 4387.5 71 3272.5 72 2473.0 73 2059.5 74 1578.5 75 1162.5 76 875.5 77 564.5 78 369.5 79 251.5 80 166.0 81 109.5 82 69.5 83 45.5 84 33.0 85 27.0 86 21.5 87 16.0 88 14.0 89 10.5 90 6.0 91 5.0 92 4.5 93 3.0 94 1.5 95 3.0 96 3.0 97 2.5 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0332730748984478 2 0.02235009576511898 3 0.021173774935375873 4 0.0495315092238257 5 0.054614895666644125 6 0.04822915401946727 7 0.05011966963869726 8 0.04108720612459842 9 0.04230553841254664 10-11 0.05032972692972282 12-13 0.04346085351318719 14-15 0.03814640405024066 16-17 0.04638064985844239 18-19 0.04904837745446693 20-21 0.01149013381909782 22-23 0.01926225358704333 24-25 0.005860598419612965 26-27 0.010859961946021159 28-29 0.006133672897946186 30-31 0.009578612470765276 32-33 0.004075111445895754 34-35 0.00804519424627873 36-37 0.009956715594611275 38-39 0.01176320829743104 40-41 0.0017434755155121009 42-43 0.0018485041610248778 44-45 0.002100572910255543 46-47 2.3106302012810973E-4 48-49 9.662635387175498E-4 50-51 2.100572910255543E-4 52-53 0.0032978994691012027 54-55 0.006511776021792184 56-57 0.01651050307460857 58-59 0.011007002049739046 60-61 0.010649904654995605 62-63 0.006091661439741075 64-65 2.100572910255543E-5 66-67 0.006700827583715182 68-69 0.016804583282044344 70-71 0.006301718730766629 72-73 0.0028567791579475386 74-75 0.004075111445895754 76-77 0.006889879145638181 78-79 0.0058395926905104095 80-81 0.010460853093072604 82-83 0.006154678627048741 84-85 0.01737173796781334 86-87 0.004516231757049418 88-89 0.006511776021792184 90-91 0.0038650541548701993 92-93 0.016930617656659677 94-95 0.011385105173585043 96-97 0.009494589554355054 98-99 0.02138383222640143 100-101 0.03678103165857456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2380303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.85300873466682 #Duplication Level Percentage of deduplicated Percentage of total 1 78.84980900576409 46.40548498143045 2 12.350496692832804 14.537277794815253 3 3.9141968527253415 6.910867846879497 4 1.610376764391717 3.791020711234008 5 0.8720931297117998 2.566265229018574 6 0.5058889501785552 1.7863852082177962 7 0.34058599574848397 1.4031157407883508 8 0.25110339175745217 1.1822552086724638 9 0.1840454523870342 0.9748465755218847 >10 1.0015954990079086 10.986142044970892 >50 0.0799935630754254 3.2164032734736026 >100 0.036519056438213224 3.9212858398364983 >500 0.002364267769118381 0.9621193911809744 >1k 7.880892563720664E-4 0.8728983975902705 >5k 1.4328895570401206E-4 0.4836317563694524 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6312 0.2651763241906598 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5158 0.21669510142196183 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4094 0.17199490989172386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2918 0.1225894350425135 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2835 0.11910248401148929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.402291641022172E-5 2 0.0 0.0 0.0 0.0 8.402291641022172E-5 3 0.0 0.0 0.0 0.0 8.402291641022172E-5 4 0.0 0.0 0.0 4.201145820511086E-5 8.402291641022172E-5 5 0.0 0.0 0.0 4.201145820511086E-5 8.402291641022172E-5 6 0.0 0.0 0.0 4.201145820511086E-5 1.260343746153326E-4 7 4.201145820511086E-5 0.0 0.0 4.201145820511086E-5 1.260343746153326E-4 8 4.201145820511086E-5 0.0 0.0 4.201145820511086E-5 1.260343746153326E-4 9 4.201145820511086E-5 0.0 0.0 4.201145820511086E-5 1.6804583282044343E-4 10-11 4.201145820511086E-5 0.0 0.0 4.201145820511086E-5 1.6804583282044343E-4 12-13 4.201145820511086E-5 0.0 0.0 4.201145820511086E-5 3.7810312384599777E-4 14-15 4.201145820511086E-5 0.0 0.0 4.201145820511086E-5 4.4112031115366406E-4 16-17 6.30171873076663E-5 0.0 0.0 4.201145820511086E-5 5.461489566664413E-4 18-19 1.47040103717888E-4 0.0 0.0 4.201145820511086E-5 6.721833312817737E-4 20-21 2.520687492306652E-4 0.0 0.0 6.30171873076663E-5 8.192234349996617E-4 22-23 2.520687492306652E-4 0.0 0.0 1.6804583282044343E-4 8.612348932047727E-4 24-25 2.520687492306652E-4 0.0 0.0 3.570973947434423E-4 8.822406223073281E-4 26-27 2.520687492306652E-4 0.0 0.0 7.141947894868846E-4 9.242520805124389E-4 28-29 3.7810312384599777E-4 0.0 0.0 0.001218332287948215 9.242520805124389E-4 30-31 4.6212604025621946E-4 0.0 0.0 0.002919796345255205 9.242520805124389E-4 32-33 5.041374984613304E-4 0.0 0.0 0.005608529670382299 9.242520805124389E-4 34-35 6.30171873076663E-4 0.0 0.0 0.00827625726640684 0.0010082749969226607 36-37 7.141947894868846E-4 0.0 0.0 0.010166772885636829 0.0010082749969226607 38-39 7.772119767945509E-4 0.0 0.0 0.011616168193713153 0.0010502864551277715 40-41 7.982177058971063E-4 0.0 0.0 0.013800764020378917 0.0010922979133328825 42-43 7.982177058971063E-4 0.0 0.0 0.016447485887300904 0.001113303642435438 44-45 7.982177058971063E-4 0.0 0.0 0.019241247857940777 0.0011973265588456597 46-47 9.662635387175498E-4 0.0 0.0 0.02340038222024675 0.0013023552043584367 48-49 0.0011343093715379933 0.0 0.0 0.028252705642937054 0.0014283895789737693 50-51 0.0011343093715379933 0.0 0.0 0.03333609208575547 0.0014283895789737693 52-53 0.0011343093715379933 0.0 0.0 0.04625461548382706 0.0014914067662814357 54-55 0.0011343093715379933 0.0 0.0 0.06177784929061552 0.001512412495383991 56-57 0.0011343093715379933 0.0 0.0 0.08043093673368473 0.001512412495383991 58-59 0.0011343093715379933 0.0 0.0 0.10889369966764735 0.001512412495383991 60-61 0.0011343093715379933 0.0 0.0 0.14932972819006657 0.001512412495383991 62-63 0.0011343093715379933 0.0 0.0 0.17529280936082509 0.0015964354117942127 64-65 0.0011343093715379933 0.0 0.0 0.20180203948825004 0.0017014640573069899 66-67 0.0011343093715379933 0.0 0.0 0.2583914736905344 0.0017644812446146563 68-69 0.0011343093715379933 0.0 0.0 0.32867664326768486 0.0017644812446146563 70-71 0.0011343093715379933 0.0 0.0 0.3805187826927916 0.0017644812446146563 72-73 0.001176320829743104 0.0 0.0 0.4261012148453369 0.0019325270774350996 74-75 0.0012183322879482149 0.0 0.0 0.5273278233905516 0.0020165499938453214 76-77 0.0012183322879482149 0.0 0.0 0.5974869585930866 0.0020165499938453214 78-79 0.0012183322879482149 0.0 0.0 0.6544755016483196 0.002037555722947877 80-81 0.0012183322879482149 0.0 0.0 0.7266721925738027 0.0020795671811529876 82-83 0.0013023552043584367 0.0 0.0 0.8595544348765682 0.002226607284870876 84-85 0.0013653723916661029 0.0 0.0 1.002540432877663 0.002226607284870876 86-87 0.0013863781207686585 0.0 0.0 1.1942387166675839 0.0022476130139734312 88-89 0.0013863781207686585 0.0 0.0 1.4495423481800427 0.0023106302012810974 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTA 155 1.4069683E-6 24.52558 7 GTACATG 9365 0.0 24.406094 1 TACATGG 9190 0.0 23.992338 2 GTATCAA 13330 0.0 22.99273 1 GGTATCA 10260 0.0 22.50867 1 ACATGGG 9325 0.0 22.472937 3 GAGTACT 7315 0.0 21.98714 12-13 GTACTTT 7640 0.0 20.895908 14-15 TAGCCCT 1645 0.0 19.643652 4 TCAACGC 15450 0.0 19.561756 4 ATCAACG 15495 0.0 19.535204 3 CAACGCA 15570 0.0 19.379656 5 TATCAAC 15830 0.0 19.151812 2 AACGCAG 15815 0.0 19.079033 6 AGTACTT 7780 0.0 18.871365 12-13 ACCGTAT 205 1.5855754E-5 18.54334 8 GTGTAGC 1470 0.0 18.425465 1 AGAGTAC 12480 0.0 18.351652 10-11 CATGGGG 5490 0.0 18.090607 4 GTACCGT 295 6.3202606E-8 17.718319 6 >>END_MODULE