FastQCFastQC Report
Thu 26 May 2016
SRR936657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380303
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68840.28920687828398317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64820.2723182720855286No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51780.21753533058606403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37230.15640865889762773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153450.023.5954191
GGTATCA116400.021.5944581
GTACATG90150.021.346611
GAGTACT90550.021.1680412-13
TACATGG88350.021.0201822
TCAACGC176350.020.3885654
ATCAACG177800.020.222293
GTACTTT95600.019.99953314-15
TATCAAC180500.019.9724252
CAACGCA180400.019.9308415
AACGCAG181650.019.7949336
ACATGGG89900.019.548273
AGTACTT95050.019.46618812-13
AGAGTAC143550.019.0781110-11
ATAGCCC7800.018.268083
TAAGGTG11050.018.0531625
ACGCAGA201300.017.8154497
CGTACAC2956.3511834E-817.7107493
TACTTTT104700.017.67146914-15
CGCAGAG203900.017.5416878
Produced by FastQC (version 0.11.5)