FastQCFastQC Report
Thu 26 May 2016
SRR936656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438061
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62270.25540788355992733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52200.214104569163774No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41540.17038129890925616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28720.11779852924106492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28190.11562467058863582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG90150.024.4597022
GTACATG92400.024.4303341
ACATGGG91100.023.5830063
GTATAGC9600.023.266681
GTATCAA137100.023.0164551
TAGTCGA1302.9277327E-421.9397475
TAGCCCT17900.020.9791954
GAGTACT74700.020.77375212-13
GGTATCA103700.020.7141671
ATAGCCC9000.020.0687453
GTACTTT77600.019.84103614-15
TCAACGC158050.019.7298644
ATCAACG157900.019.7168833
CATGGGG54350.019.678764
TATCAAC160350.019.4416792
CAACGCA160200.019.406135
AACGCAG162300.019.154646
AGTACTT77850.018.71217212-13
AGAGTAC121500.018.54400310-11
GGACCGT3851.2732926E-1118.5201846
Produced by FastQC (version 0.11.5)