FastQCFastQC Report
Thu 26 May 2016
SRR936656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438061
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70410.2887950711651595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65300.26783579245966366No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53310.2186573674735784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.1550002235382954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1554.6011337E-827.5749284
CGTATAG1252.2569437E-422.799951
GTATCAA153500.022.7751941
TACATGG91350.022.6696012
GTACATG91550.022.621471
GAGTACT89900.021.76635412-13
ACATGGG91100.021.7394243
GTACTTT93400.020.9748314-15
GGTATCA118650.020.4571821
TAAGGTG12450.020.2166925
GTAAGGT10950.019.9502014
CGTACAC2902.4465407E-919.652313
TCAACGC177400.019.5421714
ATCAACG178450.019.4017683
CAACGCA179200.019.372385
TATCAAC181950.019.1867432
GTATAGG7200.019.1319031
AGAGTAC142650.019.07752610-11
AACGCAG181750.019.0483236
AGTACTT93850.019.0030712-13
Produced by FastQC (version 0.11.5)