##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936656_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2438061 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88389667034582 31.0 31.0 34.0 30.0 34.0 2 32.0488100995012 31.0 31.0 34.0 30.0 34.0 3 32.13438384027307 33.0 31.0 34.0 30.0 34.0 4 35.82842389915593 37.0 35.0 37.0 35.0 37.0 5 35.6564433785701 37.0 35.0 37.0 33.0 37.0 6 35.73811442781784 37.0 35.0 37.0 33.0 37.0 7 35.685189993195415 37.0 35.0 37.0 33.0 37.0 8 35.649802445467934 37.0 35.0 37.0 33.0 37.0 9 37.28466802102162 39.0 37.0 39.0 34.0 39.0 10-11 37.17335066677987 39.0 37.0 39.0 33.0 39.0 12-13 37.06810453060855 39.0 37.0 39.0 33.0 39.0 14-15 38.21180315012627 40.0 38.0 41.0 33.0 41.0 16-17 38.10469877496912 40.0 37.0 41.0 33.0 41.0 18-19 38.24023352984195 40.0 37.5 41.0 33.5 41.0 20-21 38.2515507200189 40.0 38.0 41.0 33.0 41.0 22-23 38.163950573837155 40.0 37.5 41.0 33.0 41.0 24-25 38.02974371847136 40.0 37.0 41.0 33.0 41.0 26-27 37.83241026373007 40.0 37.0 41.0 33.0 41.0 28-29 37.75603379078703 40.0 37.0 41.0 32.5 41.0 30-31 37.734893835716164 40.0 37.0 41.0 33.0 41.0 32-33 37.58779476805543 40.0 36.5 41.0 32.5 41.0 34-35 37.42410854363365 40.0 36.0 41.0 32.0 41.0 36-37 37.22733454987386 39.0 36.0 41.0 31.0 41.0 38-39 37.053491893763116 39.0 36.0 41.0 31.0 41.0 40-41 36.8639730507153 39.0 35.5 41.0 31.0 41.0 42-43 36.58759665980466 39.0 35.0 41.0 30.0 41.0 44-45 36.32953933474183 39.0 35.0 40.0 30.0 41.0 46-47 35.980534121172525 39.0 35.0 40.0 29.5 41.0 48-49 35.61392372873361 38.0 35.0 40.0 28.0 41.0 50-51 35.3165394549193 38.0 34.5 40.0 27.0 41.0 52-53 34.95027831543182 38.0 34.0 40.0 26.0 41.0 54-55 34.75846461593865 38.0 34.0 40.0 26.0 41.0 56-57 34.42933708385475 38.0 33.5 40.0 26.0 41.0 58-59 34.04672073422281 37.0 33.0 40.0 25.0 41.0 60-61 33.61933561137313 36.5 33.0 39.5 23.0 41.0 62-63 33.28291580891536 36.0 32.0 39.0 23.0 40.0 64-65 32.84107985813316 35.5 31.5 39.0 22.0 40.0 66-67 32.40928898005423 35.0 31.0 38.5 21.5 40.0 68-69 32.04098625916251 35.0 31.0 38.0 20.5 40.0 70-71 31.671186036772667 35.0 31.0 37.0 19.5 39.5 72-73 31.20542574611546 34.5 30.0 37.0 18.0 39.0 74-75 30.704485244626774 34.0 30.0 36.0 17.5 39.0 76-77 29.227548039200002 32.5 28.0 35.0 13.5 37.0 78-79 30.00221590025844 34.0 29.0 35.0 13.5 37.0 80-81 29.787457122688892 34.0 29.0 35.0 8.0 37.0 82-83 29.499146452857417 34.0 29.0 35.0 7.0 36.5 84-85 29.12778884531601 34.0 29.0 35.0 6.0 36.0 86-87 28.752876158553867 33.5 29.0 35.0 2.0 36.0 88-89 28.29261244899123 33.0 27.5 35.0 2.0 35.0 90-91 27.81656775609798 33.0 27.0 35.0 2.0 35.0 92-93 27.1884360153417 32.5 26.0 34.5 2.0 35.0 94-95 26.63403171618758 32.0 25.0 34.0 2.0 35.0 96-97 25.9140517813131 32.0 24.5 34.0 2.0 35.0 98-99 25.006477893703234 32.0 20.5 34.0 2.0 35.0 100-101 21.750777564630255 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.309604775807113 1101 2 0.2971146894767429 1101 3 0.3088902854461466 1101 4 0.2762883703440977 1101 5 0.26513822586165503 1101 6 0.30065935398007326 1101 7 0.2934629770121049 1101 8 0.22384027479346713 1101 9 0.1804005616841593 1101 10-11 0.33505344772371615 1101 12-13 0.3053794425756209 1101 14-15 0.4543443497543578 1101 16-17 0.39317742665249966 1101 18-19 0.3678333481595004 1101 20-21 0.379017743377176 1101 22-23 0.3121849838884998 1101 24-25 0.38982470551112414 1101 26-27 0.42898332205655265 1101 28-29 0.3656423445775445 1101 30-31 0.4456170890134459 1101 32-33 0.29610981144164583 1101 34-35 0.3216931242644776 1101 36-37 0.3792947145981671 1101 38-39 0.29589866807802423 1101 40-41 0.2998514786697939 1101 42-43 0.4272306050928165 1101 44-45 0.5354537729532325 1101 46-47 0.5178162549280216 1101 48-49 0.4642443959617637 1101 50-51 0.48460258157768266 1101 52-53 0.5818073362098417 1101 54-55 0.5349902791569008 1101 56-57 0.5036947250659836 1101 58-59 0.40532563973386004 1101 60-61 0.5624120016099994 1101 62-63 0.5740861468409548 1101 64-65 0.3753918716779978 1101 66-67 0.2537012087941619 1101 68-69 0.27015994519674535 1101 70-71 0.35090158488515755 1101 72-73 0.4155515852799141 1101 74-75 0.3113460651317155 1101 76-77 0.23470403777739435 1101 78-79 0.4080864969350415 1101 80-81 0.49273427948729065 1101 82-83 0.4151841423752387 1101 84-85 0.4350266794916955 1101 86-87 0.4435524431335125 1101 88-89 0.3090971284649022 1101 90-91 0.41360459549760265 1101 92-93 0.4362335501934602 1101 94-95 0.27793779970944854 1101 96-97 0.35287116315290845 1101 98-99 0.17237717139266806 1101 100-101 0.7053074425035852 1102 1 0.30349751569694305 1102 2 0.26761812749576563 1102 3 0.2175235754570366 1102 4 0.2151940974608877 1102 5 0.2825881922450435 1102 6 0.19076859369975807 1102 7 0.21330045132439324 1102 8 0.20877090925070974 1102 9 0.3269821549910006 1102 10-11 0.3973980303169711 1102 12-13 0.3777287207215281 1102 14-15 0.38761123140677967 1102 16-17 0.3424496159903683 1102 18-19 0.27807839770994036 1102 20-21 0.3215120513661489 1102 22-23 0.3153710526083273 1102 24-25 0.2925078875116327 1102 26-27 0.28603086500248054 1102 28-29 0.2742104925083666 1102 30-31 0.37257222272263135 1102 32-33 0.4795442058262509 1102 34-35 0.48763002642905207 1102 36-37 0.46749816826902446 1102 38-39 0.3011745428861019 1102 40-41 0.28654849005508964 1102 42-43 0.37496549203519436 1102 44-45 0.41245994941095887 1102 46-47 0.3728616927962207 1102 48-49 0.36019348375086224 1102 50-51 0.22466717869482977 1102 52-53 0.1427397677773925 1102 54-55 0.11777794195665336 1102 56-57 0.11567237651657081 1102 58-59 0.2852663148161909 1102 60-61 0.44404592719973834 1102 62-63 0.3475057068277465 1102 64-65 0.23592293171326872 1102 66-67 0.05834999602985391 1102 68-69 0.14345314982588775 1102 70-71 0.18098290476002177 1102 72-73 0.3854100026442069 1102 74-75 0.10646288046975982 1102 76-77 0.05612108079587941 1102 78-79 -0.013396511944687717 1102 80-81 0.1236234752170624 1102 82-83 0.011746123353496785 1102 84-85 0.062411446764013334 1102 86-87 0.15155592075739932 1102 88-89 0.135573708683129 1102 90-91 0.15679170344905557 1102 92-93 0.20564395733779506 1102 94-95 -0.1306055714185419 1102 96-97 0.14304845987064496 1102 98-99 0.19087270618189933 1102 100-101 0.9878747121569482 1103 1 0.15304273383538103 1103 2 0.219537432053869 1103 3 0.13105640783071948 1103 4 0.10449365782466202 1103 5 0.15911004292113518 1103 6 0.19882569116081328 1103 7 0.09247218505839072 1103 8 0.08805555259161935 1103 9 0.17564060724033226 1103 10-11 0.15146388748059536 1103 12-13 0.20050578902168326 1103 14-15 0.21602717566576501 1103 16-17 0.18112858629030626 1103 18-19 0.17229530481225908 1103 20-21 0.06821662128405137 1103 22-23 -0.03183875287344051 1103 24-25 0.027462682391451665 1103 26-27 -0.16953094919708178 1103 28-29 -0.17361745814416452 1103 30-31 -0.06312061234361721 1103 32-33 -0.12047363871792527 1103 34-35 -0.003496789856605176 1103 36-37 -0.006081174226203245 1103 38-39 0.07568923161311147 1103 40-41 0.07817609664448355 1103 42-43 0.09028500145628726 1103 44-45 0.154550593344986 1103 46-47 0.20069918170688794 1103 48-49 0.10777376991130438 1103 50-51 0.24983643197368366 1103 52-53 0.26154906040314785 1103 54-55 0.2942775028284572 1103 56-57 0.14032020078400365 1103 58-59 0.2733032943000424 1103 60-61 0.24264090748501133 1103 62-63 0.30796600716222144 1103 64-65 0.475249766923028 1103 66-67 0.21203400534966477 1103 68-69 0.11690281773320521 1103 70-71 0.14842054887376577 1103 72-73 0.2304154671389611 1103 74-75 0.2066349755399024 1103 76-77 0.18567132842862222 1103 78-79 0.04054532246175313 1103 80-81 0.08811270530436488 1103 82-83 0.23055382838635552 1103 84-85 0.4709422819458311 1103 86-87 0.42325752187658594 1103 88-89 0.25197671850525083 1103 90-91 0.32349524460676804 1103 92-93 0.29851630026294274 1103 94-95 0.18570497699319688 1103 96-97 0.3852542360416358 1103 98-99 0.20829691925972327 1103 100-101 0.5952025329443806 1104 1 0.12231041219252603 1104 2 0.1613312947888872 1104 3 0.06822766172738426 1104 4 0.1268781442066924 1104 5 0.06709133129369604 1104 6 0.003764976735851633 1104 7 0.02834737563907197 1104 8 0.08926968819307035 1104 9 0.08405587041177398 1104 10-11 0.20105487324046578 1104 12-13 0.13232734154041026 1104 14-15 0.21023763840827314 1104 16-17 0.23496299445239544 1104 18-19 0.09527809285697941 1104 20-21 0.09254424702907471 1104 22-23 0.20958954406724928 1104 24-25 0.165934217033886 1104 26-27 0.15876671776153728 1104 28-29 0.17628198609796897 1104 30-31 0.07176451429777586 1104 32-33 0.05264650247805491 1104 34-35 0.09281860355824989 1104 36-37 0.21792557009383273 1104 38-39 0.11145921579583273 1104 40-41 0.0876323132251784 1104 42-43 0.20929347989171276 1104 44-45 0.12435010106517552 1104 46-47 0.018524817615030997 1104 48-49 0.10618102124031026 1104 50-51 0.12165548971068318 1104 52-53 0.16159393589467896 1104 54-55 0.13660710656079544 1104 56-57 0.037080067977015574 1104 58-59 0.07144582864660975 1104 60-61 0.07041266986864514 1104 62-63 -0.03481655617376589 1104 64-65 0.1217741715710261 1104 66-67 0.05698758941323234 1104 68-69 0.00450780434063347 1104 70-71 0.01249093943324553 1104 72-73 0.1941199606256454 1104 74-75 0.16004116805193647 1104 76-77 0.1905387647995802 1104 78-79 0.11648148357114252 1104 80-81 0.25542610824524914 1104 82-83 0.34077412602222523 1104 84-85 0.59638520763421 1104 86-87 0.6067322176801291 1104 88-89 0.5039057148257662 1104 90-91 0.5136644412641154 1104 92-93 0.42504237914136667 1104 94-95 0.3797687343383416 1104 96-97 0.6138163778945689 1104 98-99 0.6982204474468112 1104 100-101 1.4217121880554622 1105 1 -0.04536995527401544 1105 2 0.05685675610195062 1105 3 0.06594683492767217 1105 4 0.11590166523306777 1105 5 0.154966519382711 1105 6 0.02730976172041011 1105 7 6.923506925318179E-4 1105 8 0.05858148038440447 1105 9 0.058713350414940635 1105 10-11 0.23760353879840324 1105 12-13 0.10372593186273349 1105 14-15 0.17618758693127745 1105 16-17 0.12524572194111983 1105 18-19 0.11177052969866708 1105 20-21 0.1395506618544502 1105 22-23 0.21500254794782592 1105 24-25 0.12822533923235113 1105 26-27 0.11061196999880707 1105 28-29 0.17809160041649363 1105 30-31 0.27576388073477887 1105 32-33 0.1800401826871294 1105 34-35 0.22987411951611847 1105 36-37 0.1384332124475236 1105 38-39 0.1239681252755247 1105 40-41 0.22787148325764406 1105 42-43 0.22453858957730688 1105 44-45 0.3161217016037057 1105 46-47 0.21589553116535853 1105 48-49 0.33140870794340316 1105 50-51 0.334318830171604 1105 52-53 0.3653398771317171 1105 54-55 0.319576141169172 1105 56-57 0.1638164265736748 1105 58-59 0.11731974960962788 1105 60-61 0.2420607256221956 1105 62-63 0.290989256766764 1105 64-65 0.17196424047099867 1105 66-67 0.21044050776280443 1105 68-69 0.12290488995083848 1105 70-71 0.005747453252837431 1105 72-73 0.13778037085768702 1105 74-75 0.010504460995630183 1105 76-77 0.027091390126262382 1105 78-79 -0.005376439926131127 1105 80-81 0.07420729142414828 1105 82-83 0.24834520387128123 1105 84-85 0.3053026068580955 1105 86-87 0.46848481132866127 1105 88-89 0.33044408498494704 1105 90-91 0.1426222301292448 1105 92-93 0.09389483815776245 1105 94-95 0.3682497670460343 1105 96-97 0.3484009981527443 1105 98-99 0.12933631028042214 1105 100-101 0.8960251681774203 1106 1 0.11536753879150652 1106 2 0.13729712497141122 1106 3 0.05128700804438324 1106 4 0.08518018235496783 1106 5 0.12223908094144065 1106 6 0.01147623453880442 1106 7 -0.012947630208309135 1106 8 -0.012326858628341597 1106 9 0.10682581234245703 1106 10-11 -0.0015783670634377245 1106 12-13 0.09315943238170377 1106 14-15 0.15703653355294733 1106 16-17 0.12092351753933173 1106 18-19 0.16491522485354437 1106 20-21 0.09199743519888415 1106 22-23 0.10927865046839713 1106 24-25 -0.005795305268961215 1106 26-27 -0.06772143815315701 1106 28-29 -0.2876877852817188 1106 30-31 -0.11932060310508064 1106 32-33 -0.08019643464004389 1106 34-35 -0.04571562135294727 1106 36-37 -0.2235522564136403 1106 38-39 0.05176596805029021 1106 40-41 0.054627714629511104 1106 42-43 0.10247913188995739 1106 44-45 -0.03716099992028177 1106 46-47 0.06747665292792249 1106 48-49 -0.05464673130318687 1106 50-51 0.007840379978361511 1106 52-53 -0.20592602692903483 1106 54-55 -0.03690444475594035 1106 56-57 -0.1891617631146829 1106 58-59 -0.011236005915726821 1106 60-61 -0.2705148582012029 1106 62-63 -0.0033817293836335693 1106 64-65 -0.08254834334287153 1106 66-67 -0.08426219699879312 1106 68-69 0.06415899987436546 1106 70-71 0.08908105865392812 1106 72-73 0.23128999049335874 1106 74-75 0.09068296465018122 1106 76-77 0.04164572273725753 1106 78-79 -0.03316836522720479 1106 80-81 0.1538422037027054 1106 82-83 0.11904439079679463 1106 84-85 0.3902166538523595 1106 86-87 0.5532355496525305 1106 88-89 0.23135109086569017 1106 90-91 0.36076778909925267 1106 92-93 0.5379018656678909 1106 94-95 0.4176315610880188 1106 96-97 0.5488465091027948 1106 98-99 0.6759832112246329 1106 100-101 0.8251151967581372 1107 1 0.12112092532667873 1107 2 0.09932857862960986 1107 3 0.03771885392210805 1107 4 0.10434273713036646 1107 5 0.06603308741828329 1107 6 0.0217546038708889 1107 7 0.01669087122609625 1107 8 0.15170037062549824 1107 9 0.1103952492226199 1107 10-11 0.10561385529285872 1107 12-13 0.04696492932536245 1107 14-15 0.11555113796889316 1107 16-17 0.1060878246924446 1107 18-19 0.04225748096376947 1107 20-21 0.07509424645845542 1107 22-23 -0.01665365905021332 1107 24-25 0.05437093145639693 1107 26-27 0.1445374876132064 1107 28-29 0.05262489238840118 1107 30-31 0.03248849317024849 1107 32-33 0.1546363122120269 1107 34-35 0.22589069590350874 1107 36-37 0.22082795954626278 1107 38-39 -0.03633802882761472 1107 40-41 -0.038420743180260786 1107 42-43 0.01350383802426336 1107 44-45 -0.009714873033786375 1107 46-47 -0.055249305417234496 1107 48-49 0.07201011900440335 1107 50-51 0.0868738925716741 1107 52-53 0.23289104201268174 1107 54-55 0.12317259681301351 1107 56-57 -0.06591468437835601 1107 58-59 -0.025330444073212277 1107 60-61 -0.00797168741938492 1107 62-63 0.05891804352474139 1107 64-65 0.07769896259379294 1107 66-67 0.14517535715152263 1107 68-69 0.21661735644410385 1107 70-71 0.16875880378297836 1107 72-73 0.2411268682606682 1107 74-75 0.3266765802119913 1107 76-77 0.10685670379701051 1107 78-79 0.12071407001254642 1107 80-81 0.37183060017925484 1107 82-83 0.3377578716037988 1107 84-85 0.4951356498660253 1107 86-87 0.6392663434513786 1107 88-89 0.4178053850064245 1107 90-91 0.6710359398515706 1107 92-93 0.9121716176933994 1107 94-95 0.798186690869418 1107 96-97 0.9934976804890319 1107 98-99 0.7032611120239629 1107 100-101 1.1116945869698256 1108 1 0.08846685503791818 1108 2 0.11378618366589421 1108 3 0.035129966183525596 1108 4 0.03329986724258305 1108 5 0.021260109041897124 1108 6 0.09256156746268118 1108 7 0.11252073243738181 1108 8 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-1.1884344220691645 2316 54-55 -1.055825041720034 2316 56-57 -1.0491369457854844 2316 58-59 -1.8687849797839107 2316 60-61 -1.149466516740297 2316 62-63 -0.9952682011896172 2316 64-65 -0.9514356790544625 2316 66-67 -0.8190724482055103 2316 68-69 -1.3523902915279074 2316 70-71 -1.1535276701630934 2316 72-73 -0.7816261077754589 2316 74-75 -1.093015651149532 2316 76-77 -0.8214996564321488 2316 78-79 -0.9458992648420832 2316 80-81 -0.7593739554120305 2316 82-83 -0.9075292212333146 2316 84-85 -0.7554526423750154 2316 86-87 -1.085576340668709 2316 88-89 -1.2802551349312061 2316 90-91 -1.1997783340578536 2316 92-93 -1.1950580842362264 2316 94-95 -1.191554930763413 2316 96-97 -1.5082794703302191 2316 98-99 -2.3933916901003194 2316 100-101 -3.0514206304891225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 31.0 5 75.0 6 166.0 7 396.0 8 1022.0 9 1831.0 10 3025.0 11 4744.0 12 6600.0 13 9019.0 14 11707.0 15 15216.0 16 18761.0 17 22980.0 18 20986.0 19 15784.0 20 15225.0 21 15683.0 22 16782.0 23 19082.0 24 22199.0 25 26603.0 26 33032.0 27 40812.0 28 50239.0 29 62409.0 30 77256.0 31 93817.0 32 117536.0 33 148918.0 34 193801.0 35 262483.0 36 368958.0 37 462869.0 38 263362.0 39 14643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.179277471622456 18.966482727329044 14.827786094317352 23.026453706731147 2 16.16689139769023 20.130170334766497 38.97024648761204 24.73269177993124 3 20.102369436512777 23.338352810298638 29.240059938379805 27.319217814808784 4 13.478046693663531 16.447332531876764 35.937739047546394 34.136881726913316 5 14.208660241707111 37.112385930716755 33.7675021082924 14.911451719283738 6 33.34498193441428 35.50370560867837 16.50947207637545 14.641840380531907 7 29.77353724947817 32.05231534403774 19.56677868191157 18.607368724572517 8 27.07003639367514 34.5725558138209 18.795099876500217 19.562307916003743 9 26.24794867724803 16.687728485874636 19.660459684970967 37.40386315190637 10-11 25.75483140085502 26.120839470382407 26.392202656127147 21.73212647263543 12-13 26.631169405935985 23.208978073882236 27.11092179603315 23.04893072414863 14-15 23.18524248652511 24.99943602538583 25.4687039207105 26.346617567378562 16-17 22.839814680562682 27.991819727197086 26.172525251116614 22.99584034112361 18-19 22.65029824314943 26.608005857193984 28.50412885006424 22.237567049592343 20-21 23.49799697382469 25.629793512139358 28.578325152652045 22.293884361383903 22-23 23.00956498199388 25.52156222570404 28.614615719055315 22.85425707324676 24-25 23.03303731941079 25.71258471383612 28.77544901460628 22.47892895214681 26-27 22.81570123358046 25.83429210118846 28.759446683278473 22.590559981952605 28-29 22.60971591551153 26.27183176957899 28.58995085909927 22.528501455810208 30-31 22.856757885825417 25.798252236078355 28.46370398717489 22.881285890921337 32-33 22.746026042384507 25.796842492140687 28.549796707948573 22.907334757526236 34-35 23.29496129333005 25.86795454419607 28.323106098748717 22.513978063725162 36-37 23.26062480389 25.661605917856317 28.501483770808168 22.576285507445515 38-39 22.923566441382548 25.956360302826155 28.47466143293824 22.64541182285306 40-41 23.140510952273235 25.88815143871827 28.362561885896366 22.608775723112128 42-43 22.99646115525694 25.97243372228836 27.951294995677706 23.079810126776984 44-45 23.1452350048164 25.958434790134387 27.943012877844335 22.953317327204875 46-47 23.551099728636256 26.360694750243635 27.454127386737632 22.634078134382477 48-49 23.597017695512495 26.358182110889967 27.274427590110527 22.77037260348701 50-51 23.381571159680714 26.619515770537138 27.30169778500028 22.69721528478187 52-53 23.68226980840473 26.638764655118667 26.91529644930223 22.76366908717437 54-55 23.36866987266582 26.54489776288964 27.043516834366716 23.042915530077828 56-57 23.41622200366533 26.69124577087284 26.89494125115503 22.997590974306803 58-59 23.65162404923359 26.43956788296252 26.889158160308668 23.019649907495214 60-61 23.415479054143727 26.543424496273627 27.35437939233048 22.686717057252164 62-63 23.15864629854566 26.62619460473695 27.332507155219343 22.882651941498057 64-65 23.2032759863424 26.71867647788804 27.110329473416044 22.96771806235352 66-67 23.16534669892533 26.67457341597206 26.893851377860106 23.26622850724251 68-69 23.221729133968722 26.561467214074607 27.04876528664164 23.168038365315024 70-71 23.42446324082528 26.48056362892105 26.913346606192306 23.181626524061365 72-73 23.376059270111853 26.524876248867695 26.933090407797845 23.165974073222607 74-75 23.24763870644899 27.074190402652974 26.530326381988644 23.14784450890939 76-77 23.426445570819617 26.709946544463048 26.681439659229923 23.182168225487413 78-79 23.38888593773298 26.762959579578556 26.69094909652584 23.157205386162623 80-81 23.575019537139024 26.7813050603754 26.519620210324224 23.124055192161354 82-83 23.54883504365894 26.866381281934 26.595997366310513 22.988786308096543 84-85 23.438197798636022 26.965467653940085 26.48710024504047 23.109234302383427 86-87 23.375016920924928 26.97718444013274 26.341552458969318 23.30624617997301 88-89 23.327520342050086 27.015637630433265 26.13743142379989 23.519410603716757 90-91 23.377327903823232 26.869714532160245 26.378522644992398 23.374434919024125 92-93 23.442979828895997 26.82113920539865 26.49053397381088 23.24534699189447 94-95 23.14343857892804 26.7442452042514 26.666844457938343 23.445471758882213 96-97 23.210844500764924 26.719044437732677 26.672324248373307 23.39778681312909 98-99 23.011391733391008 26.880042285536533 26.24541041470112 23.863155566371343 100-101 22.858729949945015 26.927336448166635 26.33290658106176 23.881027020826593 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 154.0 2 135.0 3 177.5 4 176.0 5 181.0 6 294.5 7 623.0 8 1027.0 9 1297.5 10 1362.5 11 1308.0 12 1403.0 13 1597.0 14 1783.0 15 1995.5 16 2224.0 17 2443.5 18 2772.5 19 3209.5 20 3652.5 21 4171.0 22 4995.5 23 6050.0 24 7269.5 25 9020.0 26 11595.5 27 14924.0 28 18032.5 29 21679.5 30 25952.0 31 29942.0 32 35086.0 33 39078.5 34 41666.5 35 45409.0 36 50430.0 37 54659.0 38 57412.0 39 60341.0 40 62997.5 41 65714.5 42 68283.0 43 72011.5 44 76358.5 45 80394.5 46 83902.5 47 89861.5 48 100699.0 49 101979.0 50 93761.0 51 89902.0 52 89676.5 53 90443.0 54 91208.5 55 86879.5 56 78664.5 57 71800.5 58 68278.5 59 62569.0 60 52363.5 61 43098.5 62 35653.5 63 28260.0 64 20634.0 65 15241.0 66 11696.5 67 9266.5 68 7147.0 69 5665.5 70 4576.0 71 3286.0 72 2484.0 73 2113.5 74 1650.5 75 1187.5 76 891.0 77 579.0 78 379.5 79 278.0 80 198.5 81 141.5 82 93.5 83 61.0 84 42.5 85 23.0 86 12.5 87 7.5 88 4.0 89 2.0 90 4.0 91 3.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01976980887680825 2 0.013617378728423942 3 0.009187629021587236 4 0.0 5 0.002830117868256783 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0013330265321499339 14-15 3.281296079138299E-4 16-17 2.0508100494614367E-5 18-19 0.0013740427331391625 20-21 0.0 22-23 4.1016200989228734E-5 24-25 0.0 26-27 0.0014765832356122344 28-29 0.0029326583707298547 30-31 0.001292010331160705 32-33 0.007752061986964231 34-35 0.0018252209440206787 36-37 6.562592158276597E-4 38-39 0.001168961728193019 40-41 2.2558910544075806E-4 42-43 0.004860419817223605 44-45 0.0014970913361068488 46-47 2.0508100494614367E-4 48-49 5.53718713354588E-4 50-51 8.203240197845747E-5 52-53 2.0508100494614367E-4 54-55 0.0011279455272037902 56-57 0.01449922704969236 58-59 0.0254095365128272 60-61 0.02061064099708744 62-63 0.013535346326445482 64-65 0.01821119323921756 66-67 0.0024404639588591097 68-69 0.0010459131252253328 70-71 1.230486029676862E-4 72-73 1.0254050247307184E-4 74-75 0.0017842047430314501 76-77 0.0021943667529237374 78-79 0.009085088519114165 80-81 0.016242415591734578 82-83 0.01706273961151915 84-85 0.02905997840086856 86-87 0.010295066448296412 88-89 0.04064705518032568 90-91 0.04640983141931231 92-93 0.02924455130532009 94-95 0.030598085937964634 96-97 0.049752651799934454 98-99 0.05307496408006199 100-101 0.059083837524984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2438061.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.71449553435863 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53574974864425 42.64150687589629 2 13.555394354517277 15.104639164624462 3 4.315728078230744 7.213458382262789 4 1.8659909347830037 4.158509744124854 5 0.9931125771736258 2.7665383123027683 6 0.604148719148049 2.0195904669037477 7 0.3822297055099478 1.4907014654513242 8 0.2759795474186188 1.2300849009783161 9 0.19170385303881185 0.9612615117645202 >10 1.1415534399349532 11.790270445120647 >50 0.09044854960930926 3.4417496197911928 >100 0.0437673444150362 4.336782250707595 >500 0.0028689957101572065 1.0817793993081923 >1k 0.0011034598885203853 0.9884137301540943 >5k 2.2069197770407706E-4 0.7747137306092506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7041 0.2887950711651595 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6530 0.26783579245966366 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5331 0.2186573674735784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3779 0.1550002235382954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.1016200989228734E-5 2 0.0 0.0 0.0 0.0 4.1016200989228734E-5 3 0.0 0.0 0.0 0.0 4.1016200989228734E-5 4 0.0 0.0 0.0 0.0 4.1016200989228734E-5 5 0.0 0.0 0.0 0.0 4.1016200989228734E-5 6 0.0 0.0 0.0 0.0 8.203240197845747E-5 7 4.1016200989228734E-5 0.0 0.0 0.0 8.203240197845747E-5 8 1.230486029676862E-4 0.0 0.0 0.0 1.230486029676862E-4 9 1.230486029676862E-4 0.0 0.0 0.0 1.230486029676862E-4 10-11 1.230486029676862E-4 0.0 0.0 0.0 1.230486029676862E-4 12-13 1.230486029676862E-4 0.0 0.0 0.0 2.0508100494614367E-4 14-15 2.0508100494614367E-4 0.0 0.0 2.0508100494614367E-5 2.460972059353724E-4 16-17 2.0508100494614367E-4 0.0 0.0 8.203240197845747E-5 2.8711340692460114E-4 18-19 3.076215074192155E-4 0.0 0.0 8.203240197845747E-5 2.8711340692460114E-4 20-21 3.281296079138299E-4 0.0 0.0 1.0254050247307184E-4 4.1016200989228734E-4 22-23 3.691458089030586E-4 0.0 0.0 1.6406480395691494E-4 4.1016200989228734E-4 24-25 3.691458089030586E-4 0.0 0.0 2.8711340692460114E-4 4.1016200989228734E-4 26-27 3.691458089030586E-4 0.0 0.0 6.767673163222741E-4 4.1016200989228734E-4 28-29 4.511782108815161E-4 0.0 0.0 0.0014355670346230059 4.1016200989228734E-4 30-31 4.511782108815161E-4 0.0 0.0 0.0032607879786436846 4.1016200989228734E-4 32-33 4.511782108815161E-4 0.0 0.0 0.00535261422909435 4.3067011038690174E-4 34-35 4.716863113761304E-4 0.0 0.0 0.007649521484491159 5.332106128599736E-4 36-37 5.332106128599736E-4 0.0 0.0 0.009044072318124936 5.332106128599736E-4 38-39 5.742268138492023E-4 0.0 0.0 0.010254050247307184 6.152430148384311E-4 40-41 5.742268138492023E-4 0.0 0.0 0.012140795492811706 6.152430148384311E-4 42-43 6.972754168168885E-4 0.0 0.0 0.014396686547219286 6.152430148384311E-4 44-45 7.79307818795346E-4 0.0 0.0 0.017165280113992227 6.152430148384311E-4 46-47 8.818483212684179E-4 0.0 0.0 0.02089775440401204 6.767673163222741E-4 48-49 9.023564217630322E-4 4.1016200989228734E-5 0.0 0.025081406904913374 7.382916178061172E-4 50-51 9.023564217630322E-4 4.1016200989228734E-5 0.0 0.030557069736975407 7.382916178061172E-4 52-53 9.023564217630322E-4 4.1016200989228734E-5 0.0 0.04165195210456178 8.203240197845747E-4 54-55 9.023564217630322E-4 4.1016200989228734E-5 0.0 0.056704897867608725 8.203240197845747E-4 56-57 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.07428033999149324 8.408321202791891E-4 58-59 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.10038715192113733 8.613402207738035E-4 60-61 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.1359892143797879 8.613402207738035E-4 62-63 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.16217805871141042 9.023564217630322E-4 64-65 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.18824385444006528 9.228645222576465E-4 66-67 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.24501027660915786 9.433726227522609E-4 68-69 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.31375342946710516 9.638807232468752E-4 70-71 9.843888237414896E-4 4.1016200989228734E-5 0.0 0.3634445569655558 0.0010459131252253328 72-73 0.0010254050247307184 4.1016200989228734E-5 0.0 0.40831628084777205 0.0012099779291822477 74-75 0.0010664212257199472 4.1016200989228734E-5 0.0 0.5060783959055988 0.0012715022306660907 76-77 0.0010664212257199472 4.1016200989228734E-5 0.0 0.575313743175417 0.0012715022306660907 78-79 0.0010664212257199472 4.1016200989228734E-5 0.0 0.6302344362999941 0.0012715022306660907 80-81 0.0011484536276984046 4.1016200989228734E-5 0.0 0.7021973609355959 0.001292010331160705 82-83 0.0011484536276984046 4.1016200989228734E-5 0.0 0.8365459272758147 0.0014355670346230056 84-85 0.0012099779291822477 4.1016200989228734E-5 0.0 0.9747295084085262 0.0014355670346230056 86-87 0.0012715022306660907 4.1016200989228734E-5 0.0 1.1661316103247623 0.00145607513511762 88-89 0.0012715022306660907 4.1016200989228734E-5 0.0 1.4116956056472745 0.0014765832356122344 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 155 4.6011337E-8 27.574928 4 CGTATAG 125 2.2569437E-4 22.79995 1 GTATCAA 15350 0.0 22.775194 1 TACATGG 9135 0.0 22.669601 2 GTACATG 9155 0.0 22.62147 1 GAGTACT 8990 0.0 21.766354 12-13 ACATGGG 9110 0.0 21.739424 3 GTACTTT 9340 0.0 20.97483 14-15 GGTATCA 11865 0.0 20.457182 1 TAAGGTG 1245 0.0 20.216692 5 GTAAGGT 1095 0.0 19.950201 4 CGTACAC 290 2.4465407E-9 19.65231 3 TCAACGC 17740 0.0 19.542171 4 ATCAACG 17845 0.0 19.401768 3 CAACGCA 17920 0.0 19.37238 5 TATCAAC 18195 0.0 19.186743 2 GTATAGG 720 0.0 19.131903 1 AGAGTAC 14265 0.0 19.077526 10-11 AACGCAG 18175 0.0 19.048323 6 AGTACTT 9385 0.0 19.00307 12-13 >>END_MODULE