FastQCFastQC Report
Thu 26 May 2016
SRR936655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963668
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61800.6412997007268063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47870.4967478426179971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.3050843236467331No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15230.15804198126325664No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.12244881017113778No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11800.12244881017113778No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA11000.11414719592224708No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG9960.10335509739868919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88450.031.3113351
GGTATCA59250.028.382181
GTATAGG3950.026.4579661
ATCAACG105900.026.0635493
TCAACGC105650.025.9903284
CAACGCA106050.025.7144475
TATCAAC107950.025.5659432
AACGCAG107950.025.3952036
GTACATG66500.025.2878841
GTGTACG1151.2807966E-424.7847351
TACATGG67500.024.771212
AGGTAAG3650.024.7268662
GAGTACT54800.024.61762212-13
ACATGGG65600.024.1877823
TATAGGG3750.024.0674822
GTACTTT56400.023.88023814-15
CGTATAG1401.7779132E-523.7520371
ACGCAGA117800.023.2314177
GGTAAGG4500.023.225423
CGCAGAG118050.023.1017258
Produced by FastQC (version 0.11.5)