##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936655_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 963668 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.649755932541083 31.0 31.0 34.0 30.0 34.0 2 31.720959915655598 33.0 31.0 34.0 30.0 34.0 3 31.718253589410462 33.0 31.0 34.0 30.0 34.0 4 35.381215314817965 37.0 35.0 37.0 33.0 37.0 5 35.39697177866236 37.0 35.0 37.0 33.0 37.0 6 35.400037149723765 37.0 35.0 37.0 33.0 37.0 7 35.322706575293566 37.0 35.0 37.0 33.0 37.0 8 35.28977822237534 37.0 35.0 37.0 33.0 37.0 9 36.81685497494988 39.0 37.0 39.0 33.0 39.0 10-11 36.70766384273422 39.0 37.0 39.0 32.0 39.0 12-13 36.63466671094194 39.0 37.0 39.0 32.0 39.0 14-15 37.741994649609616 40.0 37.0 41.0 32.5 41.0 16-17 37.66293111320496 40.0 37.0 41.0 32.0 41.0 18-19 37.704816389046854 40.0 37.0 41.0 32.0 41.0 20-21 37.59537413300016 40.0 37.0 41.0 32.0 41.0 22-23 37.67664797419858 40.0 37.0 41.0 32.0 41.0 24-25 37.70810797909654 40.0 37.0 41.0 32.0 41.0 26-27 37.608890198699136 40.0 37.0 41.0 32.0 41.0 28-29 37.46677278896881 40.0 37.0 41.0 32.0 41.0 30-31 37.208518390150964 39.0 36.5 41.0 31.5 41.0 32-33 36.96433834059033 39.0 36.0 41.0 31.0 41.0 34-35 36.75922672538675 39.0 35.0 41.0 30.5 41.0 36-37 36.528327702071664 39.0 35.0 41.0 30.0 41.0 38-39 36.296963269507756 39.0 35.0 40.0 30.0 41.0 40-41 36.0544544386656 39.0 35.0 40.0 30.0 41.0 42-43 35.76448009065363 38.0 35.0 40.0 28.5 41.0 44-45 35.40267291224779 38.0 35.0 40.0 27.5 41.0 46-47 35.05656927489551 38.0 35.0 40.0 25.5 41.0 48-49 34.79225884848309 38.0 34.0 40.0 24.0 41.0 50-51 33.78046173578453 37.5 33.0 39.5 22.5 40.5 52-53 33.869308205730604 38.0 33.5 39.5 23.0 40.5 54-55 34.29364158610642 38.0 34.0 40.0 23.0 41.0 56-57 34.17048091251344 38.0 34.0 40.0 22.0 41.0 58-59 33.93316474138396 38.0 34.0 40.0 21.5 41.0 60-61 33.65186558026208 37.5 33.5 40.0 20.0 41.0 62-63 33.29829775399826 37.0 33.0 40.0 18.5 41.0 64-65 32.92852465786972 36.5 33.0 39.5 17.5 41.0 66-67 32.46328662983517 36.0 32.0 39.0 13.0 41.0 68-69 32.08217767944977 35.0 32.0 39.0 9.5 40.5 70-71 31.686535196769015 35.0 31.0 38.5 7.5 40.0 72-73 31.308614066255185 35.0 31.0 37.5 7.0 40.0 74-75 30.882265987871342 35.0 31.0 37.0 2.0 39.0 76-77 30.443225779002727 35.0 30.5 36.5 2.0 39.0 78-79 29.966175591593785 34.0 30.0 36.0 2.0 38.5 80-81 29.549278901032306 34.0 29.0 35.5 2.0 37.0 82-83 29.160262663074835 34.0 29.0 35.0 2.0 37.0 84-85 28.801191904265785 34.0 29.0 35.0 2.0 36.5 86-87 28.483244748191286 34.0 29.0 35.0 2.0 36.0 88-89 28.087915132597534 34.0 28.0 35.0 2.0 36.0 90-91 27.69394386863526 34.0 27.0 35.0 2.0 35.0 92-93 27.267983371866663 34.0 26.0 35.0 2.0 35.0 94-95 26.806132921296545 33.0 25.0 35.0 2.0 35.0 96-97 26.271444626157557 33.0 24.5 35.0 2.0 35.0 98-99 25.574052993354556 33.0 22.0 35.0 2.0 35.0 100-101 22.790707484320325 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3263341207089354 1101 2 0.29184389225669705 1101 3 0.2419915935148964 1101 4 0.44241243894833815 1101 5 0.3586892245290372 1101 6 0.3610054768040101 1101 7 0.4160583699650928 1101 8 0.4351989537847061 1101 9 0.3768577151080663 1101 10-11 0.6270485319715036 1101 12-13 0.6987063197433727 1101 14-15 0.6417845098433475 1101 16-17 0.6375755505659271 1101 18-19 0.5972043827506042 1101 20-21 0.512367688019566 1101 22-23 0.4281284820868123 1101 24-25 0.5770719557263533 1101 26-27 0.37427775679458364 1101 28-29 0.45077313613328585 1101 30-31 0.5004900288915906 1101 32-33 0.27107434346336134 1101 34-35 0.3555689852282029 1101 36-37 0.15040506584413293 1101 38-39 0.18655550327939352 1101 40-41 0.33564025964074773 1101 42-43 0.4003005737626424 1101 44-45 0.19410504992572442 1101 46-47 0.5066642830129169 1101 48-49 0.6893957069312577 1101 50-51 0.6317277685547964 1101 52-53 0.6970767795555162 1101 54-55 0.6207218475933516 1101 56-57 0.7047014024673999 1101 58-59 0.8068019791633887 1101 60-61 0.7573643593560604 1101 62-63 0.6911799934765028 1101 64-65 0.75959179993162 1101 66-67 0.9312958244059075 1101 68-69 0.819025581890525 1101 70-71 0.7973092594043472 1101 72-73 0.648301470164796 1101 74-75 0.7019253495428259 1101 76-77 0.6658564648183578 1101 78-79 0.6071563379198501 1101 80-81 0.597496959039912 1101 82-83 0.4590469013439282 1101 84-85 0.1371774214059549 1101 86-87 0.24397945414870392 1101 88-89 0.5385934157296077 1101 90-91 0.4709897552145641 1101 92-93 0.3370308253732226 1101 94-95 0.5524949723667021 1101 96-97 0.5135653928118415 1101 98-99 0.5981711094120179 1101 100-101 0.8028207401148926 1102 1 0.30102730437475955 1102 2 0.4078639961101409 1102 3 0.2888846343462177 1102 4 0.05806773854062186 1102 5 0.1552169465656803 1102 6 0.15836056873056492 1102 7 0.29570383579286386 1102 8 0.29368525793164224 1102 9 0.41632089200263067 1102 10-11 0.48850163635800214 1102 12-13 0.39363965678134605 1102 14-15 0.3941912403533294 1102 16-17 0.2542954199979093 1102 18-19 0.2893625832828377 1102 20-21 0.08174481432930492 1102 22-23 0.13819373522701284 1102 24-25 0.16506207462476397 1102 26-27 -0.0331419708319558 1102 28-29 0.036927142188318385 1102 30-31 0.47768873350202057 1102 32-33 0.30012696377674075 1102 34-35 0.28807608064536083 1102 36-37 0.1915209243844629 1102 38-39 -0.04910419042909808 1102 40-41 -0.17255368656374515 1102 42-43 0.012037957915417508 1102 44-45 0.29496030753207947 1102 46-47 0.5180843543478488 1102 48-49 0.48132066942175555 1102 50-51 0.5155746577880791 1102 52-53 0.6917117386089302 1102 54-55 0.7368888507649345 1102 56-57 0.6454325442995881 1102 58-59 0.6808313116899356 1102 60-61 0.6640349261867584 1102 62-63 0.6717082444535976 1102 64-65 0.6123101833908109 1102 66-67 0.7241021828052538 1102 68-69 0.868410765795101 1102 70-71 0.753797475557807 1102 72-73 0.6403326323725196 1102 74-75 0.8539892978260362 1102 76-77 0.8325884772412557 1102 78-79 0.571054351203049 1102 80-81 0.7014592272682911 1102 82-83 0.7175143221109117 1102 84-85 0.33419847817546966 1102 86-87 0.5175425164051966 1102 88-89 0.6349850951052538 1102 90-91 0.383671359818198 1102 92-93 0.4610565561651754 1102 94-95 0.10019197181306794 1102 96-97 0.24212838303364848 1102 98-99 0.3975174460363071 1102 100-101 0.7375753179195641 1103 1 0.2583019078237818 1103 2 0.20791532082812836 1103 3 0.3939873918342265 1103 4 0.4156967526738313 1103 5 0.32101415450102166 1103 6 0.40431780173398124 1103 7 0.4328650926541684 1103 8 0.40802808543736546 1103 9 0.1561294237915405 1103 10-11 0.41285035269979176 1103 12-13 0.19800603963133057 1103 14-15 0.46774739499741 1103 16-17 0.40047821163035735 1103 18-19 0.4753031222464017 1103 20-21 0.3829034023052742 1103 22-23 0.13908786584031674 1103 24-25 0.3685810593678056 1103 26-27 0.0896489052539664 1103 28-29 0.3023137523797814 1103 30-31 0.31892490284117514 1103 32-33 0.21937616419165096 1103 34-35 0.474073887188986 1103 36-37 0.6460283151438517 1103 38-39 0.399545699357823 1103 40-41 0.34311574983682647 1103 42-43 0.4566556157794466 1103 44-45 0.5258977670125589 1103 46-47 0.790102658363061 1103 48-49 0.5439125136539431 1103 50-51 0.5605442951654425 1103 52-53 0.7982672557459907 1103 54-55 0.7768843125793481 1103 56-57 0.6753421587699151 1103 58-59 0.6822921752418196 1103 60-61 0.6906451716809912 1103 62-63 0.6605602455773436 1103 64-65 0.5626030044134112 1103 66-67 0.7741529672630421 1103 68-69 0.6826285230670059 1103 70-71 0.4257231249505615 1103 72-73 0.1905808819295025 1103 74-75 0.4062845932403043 1103 76-77 0.35293629675113536 1103 78-79 0.33093084772377424 1103 80-81 0.49014401786343953 1103 82-83 0.09712463243636549 1103 84-85 0.2139981496972716 1103 86-87 0.3045886978461816 1103 88-89 0.1251480375783487 1103 90-91 0.23695614176918767 1103 92-93 -0.03331231188167649 1103 94-95 0.21109091074205466 1103 96-97 0.18802267572500853 1103 98-99 0.09888889652686572 1103 100-101 0.5077227008992082 1104 1 0.3176037884273235 1104 2 0.42059026069254557 1104 3 0.33054709006295724 1104 4 0.3629059151492271 1104 5 0.4070736340549246 1104 6 0.42306643501628827 1104 7 0.6143870694746312 1104 8 0.5353952404778255 1104 9 0.5778329007213401 1104 10-11 0.7393031736051014 1104 12-13 0.6486675309936771 1104 14-15 0.5042893258961598 1104 16-17 0.4092675730998536 1104 18-19 0.37640059655211644 1104 20-21 0.20569341979926037 1104 22-23 0.32552182604149493 1104 24-25 0.6390941251889402 1104 26-27 0.2303743590042373 1104 28-29 0.3401335643194301 1104 30-31 0.5150857928477421 1104 32-33 0.6074597526456245 1104 34-35 0.5585684749869344 1104 36-37 0.5142575957036613 1104 38-39 0.551357967015818 1104 40-41 0.44861132167150686 1104 42-43 0.6231013297527639 1104 44-45 0.6794949679747333 1104 46-47 0.4743061352928706 1104 48-49 0.8523232943230923 1104 50-51 0.8074254453950545 1104 52-53 0.8925653095400037 1104 54-55 0.882837734840443 1104 56-57 0.7557174594478155 1104 58-59 0.9646888513844019 1104 60-61 0.9915731729930357 1104 62-63 1.0738789094741321 1104 64-65 1.0690921800093065 1104 66-67 1.1189758406503216 1104 68-69 1.0460155228483252 1104 70-71 0.7345425542092521 1104 72-73 0.6283637924914984 1104 74-75 0.6487444117401964 1104 76-77 0.59115245682111 1104 78-79 0.5765655514193746 1104 80-81 0.9202555420270109 1104 82-83 0.8181242497554138 1104 84-85 0.9278347995988661 1104 86-87 0.8886391023946167 1104 88-89 1.0189595398744338 1104 90-91 0.8122786871442145 1104 92-93 0.7781817838978995 1104 94-95 0.8705718313630904 1104 96-97 0.9753327019659856 1104 98-99 0.8877351134522975 1104 100-101 1.0445694098430067 1105 1 0.32710684359119213 1105 2 0.31277502332981655 1105 3 0.31388540851216007 1105 4 0.23433265531074454 1105 5 0.19208695143588983 1105 6 0.47951218983404686 1105 7 0.39394239713917756 1105 8 0.4334168616375038 1105 9 0.4943421801584549 1105 10-11 0.48880679849650477 1105 12-13 0.3243244980491795 1105 14-15 0.42640329988810066 1105 16-17 0.33190569641737966 1105 18-19 0.4190418533710414 1105 20-21 0.39648662294985115 1105 22-23 -0.045639990819587695 1105 24-25 0.1144918662192893 1105 26-27 -0.01915490362590333 1105 28-29 -0.055529384875121934 1105 30-31 -0.04986172100376507 1105 32-33 -0.20407295683606463 1105 34-35 -0.07507108689259923 1105 36-37 0.008212786633592373 1105 38-39 0.08551313230801583 1105 40-41 0.05724269371782498 1105 42-43 0.10980104061604123 1105 44-45 0.10622231062311016 1105 46-47 0.1444552614732686 1105 48-49 0.29817496576122693 1105 50-51 0.17525862917490542 1105 52-53 0.3049440644004875 1105 54-55 0.27026384830167416 1105 56-57 0.24996606005456812 1105 58-59 0.384095195300425 1105 60-61 0.26605507702243614 1105 62-63 0.2107644939433584 1105 64-65 0.28955251985177455 1105 66-67 0.48645294472529343 1105 68-69 0.499310684437301 1105 70-71 0.35522081483111023 1105 72-73 0.15443737280171632 1105 74-75 0.34349864552788745 1105 76-77 0.44997539974865575 1105 78-79 0.5190957113703796 1105 80-81 0.6579601098174628 1105 82-83 0.2984501339013228 1105 84-85 0.2985992315093071 1105 86-87 0.4282223467080222 1105 88-89 0.44863237384158694 1105 90-91 0.5546820827202801 1105 92-93 0.45137610371444836 1105 94-95 0.46652391727800335 1105 96-97 0.45998993964206747 1105 98-99 0.7116551237073914 1105 100-101 0.820276228057196 1106 1 0.22193787119116237 1106 2 0.22172362858236738 1106 3 0.21226533175270745 1106 4 0.1462526576705585 1106 5 0.1107061989834861 1106 6 0.31777261479692953 1106 7 0.30387098003905777 1106 8 0.3212294138729348 1106 9 0.44010516977855474 1106 10-11 0.36954253556096006 1106 12-13 0.45749756079069925 1106 14-15 0.3903273401491205 1106 16-17 0.4906089795063764 1106 18-19 0.24128177418621988 1106 20-21 0.18313958959456045 1106 22-23 0.39865172132262927 1106 24-25 0.12475180610962155 1106 26-27 0.1440638149304121 1106 28-29 0.16331863337409658 1106 30-31 0.34816652463959485 1106 32-33 0.08999113173555884 1106 34-35 0.22712419413812057 1106 36-37 0.28003236586593516 1106 38-39 0.10911584478126457 1106 40-41 0.1461830396269903 1106 42-43 0.2392088915807875 1106 44-45 0.1986296142424706 1106 46-47 0.2536870442132084 1106 48-49 0.24520635454359052 1106 50-51 -0.03449231229191696 1106 52-53 -0.10060936076887828 1106 54-55 0.0774491492924696 1106 56-57 -0.054312083166372815 1106 58-59 -0.06856074077488472 1106 60-61 0.1785493003983447 1106 62-63 0.19312358528448215 1106 64-65 0.2743815481662466 1106 66-67 0.3090130789723986 1106 68-69 0.28504102998593694 1106 70-71 0.11883217994980555 1106 72-73 -0.015935182580133045 1106 74-75 -0.00927200364994718 1106 76-77 0.18992177956701184 1106 78-79 0.07400625153799112 1106 80-81 0.26550757646053214 1106 82-83 -0.049085992532184264 1106 84-85 0.043172154628589254 1106 86-87 -0.019242002098469158 1106 88-89 -0.014418208081590222 1106 90-91 -0.06652345366179802 1106 92-93 -0.04287742535221284 1106 94-95 -0.08763443268873061 1106 96-97 0.021356533220103557 1106 98-99 0.20556975749400763 1106 100-101 0.012498024202180602 1107 1 0.10453631067582947 1107 2 0.10910615069542828 1107 3 0.024218625741930566 1107 4 0.13294521771641143 1107 5 0.06832055180741747 1107 6 0.1691499467131834 1107 7 0.20168069970506508 1107 8 0.32531863802203986 1107 9 0.13362001598801498 1107 10-11 0.29848983576574284 1107 12-13 0.1632714387202583 1107 14-15 -0.054636988048734736 1107 16-17 -0.0206311664643124 1107 18-19 -0.17520811760306998 1107 20-21 0.014061800007795 1107 22-23 0.015189569147899817 1107 24-25 -0.07361912878825905 1107 26-27 -0.03925533320909125 1107 28-29 -0.04879537814111501 1107 30-31 -0.007541971787460966 1107 32-33 -0.2971357688643934 1107 34-35 -0.19040546515801537 1107 36-37 0.031587336732279425 1107 38-39 -0.2432573309452053 1107 40-41 0.002703044350589323 1107 42-43 0.06939700021201389 1107 44-45 0.16951036356633153 1107 46-47 0.2520629475292324 1107 48-49 0.3298973621387873 1107 50-51 0.3126759053852908 1107 52-53 0.1377404187485638 1107 54-55 0.24457593468273586 1107 56-57 -0.05100340470799125 1107 58-59 0.09978078684807912 1107 60-61 0.23756906750194418 1107 62-63 0.416320049498907 1107 64-65 0.46070658928170616 1107 66-67 0.35915989932880166 1107 68-69 0.5785571723044747 1107 70-71 0.4041903956972419 1107 72-73 0.30902636618380797 1107 74-75 0.371276423305666 1107 76-77 0.31275259700861113 1107 78-79 0.32378339278220025 1107 80-81 0.42179384951327137 1107 82-83 0.5017852087881174 1107 84-85 0.38366541054100267 1107 86-87 0.49500511693907256 1107 88-89 0.19562238893505324 1107 90-91 0.47066083576799755 1107 92-93 0.4188787160446452 1107 94-95 0.5373151630692483 1107 96-97 0.29937517126867874 1107 98-99 0.059701681236706605 1107 100-101 -0.14434701587639154 1108 1 0.10219432341395773 1108 2 0.23250158794571973 1108 3 0.22849354585096648 1108 4 0.18590729212052537 1108 5 0.03482188305898859 1108 6 0.23145299605675973 1108 7 0.3115311679438477 1108 8 0.2690609623015945 1108 9 0.40112859216765173 1108 10-11 0.297904338950282 1108 12-13 0.13207905620836868 1108 14-15 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58-59 -1.2216671007762798 2316 60-61 -1.4365324301289633 2316 62-63 -1.5352259535176813 2316 64-65 -1.4307227874417876 2316 66-67 -1.4026569874880863 2316 68-69 -1.210386182815352 2316 70-71 -1.097325517583375 2316 72-73 -0.8386894800614471 2316 74-75 -0.6827708366239484 2316 76-77 -1.0208759204434372 2316 78-79 -1.8700306907377673 2316 80-81 -1.8572541721818077 2316 82-83 -1.7742271775183873 2316 84-85 -1.4541013978140391 2316 86-87 -1.623182795366489 2316 88-89 -1.7988338628741403 2316 90-91 -1.7352158039321708 2316 92-93 -2.370359823193894 2316 94-95 -2.172591215954778 2316 96-97 -2.459177550519339 2316 98-99 -3.7623384790387426 2316 100-101 -5.145331597743329 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1669.0 3 684.0 4 616.0 5 724.0 6 787.0 7 1020.0 8 1302.0 9 1626.0 10 2021.0 11 2829.0 12 3924.0 13 5126.0 14 6035.0 15 8088.0 16 10773.0 17 14664.0 18 10485.0 19 6710.0 20 6432.0 21 6663.0 22 6995.0 23 7950.0 24 9060.0 25 10250.0 26 11660.0 27 13926.0 28 16361.0 29 19763.0 30 24300.0 31 29856.0 32 37997.0 33 49196.0 34 67410.0 35 94619.0 36 133986.0 37 176423.0 38 140386.0 39 21350.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60045269954014 19.182650046235132 14.779363460027628 21.4375337941971 2 15.358582441166826 20.636755738024767 40.62799666241859 23.376665158389827 3 18.503581113969823 26.764792268405557 30.440454704434856 24.29117191318977 4 12.00181009000631 17.03518881397589 38.61636055664419 32.34664053937361 5 12.539159571751545 38.49855610432588 34.38900687796746 14.57327744595512 6 29.851976587010732 37.229061507891196 18.157836271030888 14.761125634067188 7 27.523178560532003 34.235050425173775 21.295944608065952 16.94582640622827 8 26.854391968686848 32.983437284947826 20.54505313832156 19.61711760804377 9 27.49924480001993 16.153909236900265 21.06182674311055 35.28501921996926 10-11 24.380629870743306 26.8914341357678 27.41765867969442 21.310277313794472 12-13 26.18656264418613 24.874828922913718 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21.452838901485475 38-39 21.075619404193148 26.237874454687717 31.17323601074229 21.513270130376853 40-41 21.083593756891084 26.242704205708584 31.544552534129195 21.12914950327114 42-43 21.109960955234722 26.238808680650138 30.813194795760857 21.838035568354282 44-45 21.158793295069056 26.284259102640068 30.88946286459074 21.66748473770014 46-47 21.85416959603512 26.674902623901257 29.94415564402609 21.526772136037536 48-49 21.482873162090897 27.09629818722935 29.30448714955332 22.11634150112643 50-51 21.733707126509067 27.08096275091853 29.429986161765694 21.755343960806716 52-53 21.992556399052972 27.046743800760964 29.103307073703892 21.857392726482168 54-55 21.400489092957237 27.220518866088884 29.25451461441512 22.124477426538768 56-57 21.78526566150631 27.402907974333285 28.66403676398483 22.147789600175578 58-59 21.861690866977163 26.709120984948488 28.988900234946414 22.440287913127943 60-61 21.42604754114437 27.165167418236607 29.432433680503127 21.976351360115892 62-63 21.52383630644004 27.123024407302733 29.67489271444011 21.678246571817116 64-65 21.41268704729884 27.14811240479007 29.312049893115827 22.127150654795262 66-67 21.303190385254066 27.260046496180674 28.954147293258053 22.482615825307207 68-69 21.647218327825236 27.018977377778075 29.283120094552096 22.050684199844596 70-71 21.735979713507803 27.041769513679625 28.95756603621772 22.26468473659485 72-73 21.56952080473876 27.160244618166818 28.839153436407837 22.431081140686583 74-75 21.44842286200062 27.90390141648411 28.453617450162522 22.194058271352745 76-77 21.521931037775555 27.374082779372465 28.90157721208523 22.202408970766747 78-79 21.425078516365144 27.30838113530771 28.904508526487916 22.362031821839228 80-81 21.672700347274144 27.514881694166128 28.44953101374142 22.362886944818307 82-83 21.91523621859226 27.435066620432757 28.52915954643705 22.120537614537934 84-85 21.392723458954315 27.49530744109846 28.37144204916795 22.740527050779274 86-87 21.645476799261836 27.62868439803357 28.180024006937415 22.545814795767182 88-89 21.641649298780237 27.24896830602908 28.164466965034503 22.944915430156186 90-91 21.349466403480044 27.073100724939703 28.7324117649196 22.845021106660653 92-93 21.716365742434597 27.206695531727952 28.754313281924254 22.322625443913193 94-95 21.17563544601219 26.80359356570535 28.987017353956258 23.0337536343262 96-97 21.39907316592198 26.625151142455334 29.019040057291424 22.956735634331263 98-99 21.235789813569415 27.05120105567688 28.386474503295617 23.326534627458084 100-101 21.362143582208123 26.846960047976697 28.551690477612173 23.239205892203007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 247.0 1 199.5 2 227.5 3 295.5 4 260.5 5 242.0 6 394.0 7 782.5 8 1304.0 9 1589.0 10 1418.5 11 1211.0 12 1269.0 13 1348.5 14 1408.5 15 1541.5 16 1694.0 17 1767.5 18 1878.5 19 2076.5 20 2299.0 21 2602.5 22 2974.5 23 3489.5 24 4022.0 25 4791.5 26 6160.0 27 7700.5 28 8674.5 29 10062.0 30 12473.0 31 15384.5 32 19736.0 33 23021.5 34 24752.5 35 27119.5 36 31425.0 37 34843.5 38 34648.0 39 33745.0 40 31982.0 41 30416.5 42 28896.5 43 28582.5 44 29682.0 45 30934.0 46 30747.0 47 30890.0 48 34555.0 49 33635.0 50 28787.5 51 26586.5 52 26280.5 53 26874.0 54 26894.0 55 25234.0 56 23219.5 57 21147.0 58 20331.5 59 19112.5 60 16063.0 61 13854.5 62 11993.5 63 8858.5 64 5889.5 65 4545.5 66 3547.0 67 2799.5 68 2279.0 69 1759.5 70 1392.5 71 1119.5 72 896.5 73 801.5 74 633.5 75 448.5 76 341.5 77 221.5 78 140.0 79 99.0 80 73.5 81 52.5 82 30.5 83 27.0 84 17.5 85 6.5 86 6.5 87 5.5 88 5.5 89 5.0 90 2.5 91 1.5 92 2.0 93 2.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011933570482780377 2 0.009961937098668836 3 0.014631595113669854 4 0.018678632060004068 5 0.03133859378956238 6 0.0458664187251211 7 0.00643375104289029 8 0.02480107256856096 9 0.03600825180456339 10-11 0.04057413964145328 12-13 0.029730156028839806 14-15 0.0278622928228394 16-17 0.02884810951489517 18-19 0.022414358472004882 20-21 0.05043230656201098 22-23 0.011777915215613676 24-25 0.00264613954183391 26-27 8.820465139446366E-4 28-29 0.0 30-31 0.0023867140965560755 32-33 0.002542369363722776 34-35 0.0 36-37 3.113105343334011E-4 38-39 0.0 40-41 0.0014008974045003051 42-43 0.0031649904323895785 44-45 0.0019716333841115407 46-47 7.782763358335029E-4 48-49 5.188508905556686E-4 50-51 0.0034763009667229793 52-53 0.0022310588293893746 54-55 0.004929083460278851 56-57 7.782763358335029E-4 58-59 0.004565887836889883 60-61 0.0019197482950559738 62-63 1.5565526716670056E-4 64-65 8.301614248890696E-4 66-67 0.01535798636044779 68-69 0.04041848437428658 70-71 0.048305017910732745 72-73 0.01302315735294728 74-75 0.027135901576061463 76-77 0.02360771552028292 78-79 0.05115869780878892 80-81 0.07678993180223893 82-83 0.04669658015001017 84-85 0.018107896080392833 86-87 0.020390839998837774 88-89 0.02116911633467128 90-91 0.03595636671550783 92-93 0.022777554095393848 94-95 0.011674145037502543 96-97 0.017952240813226134 98-99 0.05131435307595562 100-101 0.07269100976684917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 963668.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.02498538086383 #Duplication Level Percentage of deduplicated Percentage of total 1 76.16247296360369 35.05376704720296 2 12.475624723215988 11.483808910063185 3 4.357823588075674 6.0170630080069945 4 2.0620310550088057 3.7961979664667003 5 1.1018078785984602 2.5355345752507357 6 0.7052602192582136 1.947575476863846 7 0.4977571701716428 1.6036486536259005 8 0.3578622983665162 1.317648564054499 9 0.26027603805786803 1.078128076194228 >10 1.6988186578414939 15.248765603256082 >50 0.19855301400926537 6.3330006800325025 >100 0.11445951372775934 9.76968381037175 >500 0.005666312550531234 1.8446336370197145 >1k 0.0013599150121274962 1.3258662855013612 >5k 2.2665250202124937E-4 0.6446777060895682 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6180 0.6412997007268063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4787 0.4967478426179971 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2940 0.3050843236467331 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1523 0.15804198126325664 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.12244881017113778 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1180 0.12244881017113778 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1100 0.11414719592224708 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 996 0.10335509739868919 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.556552671667006E-4 0.0 24-25 0.0 0.0 0.0 3.63195623388968E-4 0.0 26-27 0.0 0.0 0.0 0.0011414719592224708 0.0 28-29 0.0 0.0 0.0 0.0032687606105007115 0.0 30-31 0.0 0.0 0.0 0.0056035896180012205 0.0 32-33 0.0 0.0 0.0 0.008197844070779564 0.0 34-35 0.0 0.0 0.0 0.01089586870166904 0.0 36-37 0.0 0.0 0.0 0.013905203866891917 0.0 38-39 0.0 0.0 0.0 0.018004125902281697 0.0 40-41 0.0 0.0 0.0 0.025683119082505593 0.0 42-43 0.0 0.0 0.0 0.03704595358567474 0.0 44-45 0.0 0.0 0.0 0.05645097689245673 0.0 46-47 0.0 0.0 0.0 0.07829459938485038 0.0 48-49 0.0 0.0 0.0 0.1195951302730816 0.0 50-51 0.0 0.0 0.0 0.16048058044886826 0.0 52-53 0.0 0.0 0.0 0.20453102105704454 0.0 54-55 0.0 0.0 0.0 0.2762362141318379 0.0 56-57 0.0 0.0 0.0 0.35235163977635453 0.0 58-59 0.0 0.0 0.0 0.44890979050876445 0.0 60-61 0.0 0.0 0.0 0.562641905718567 0.0 62-63 0.0 0.0 0.0 0.7121747323767107 0.0 64-65 0.0 0.0 0.0 0.8271520897238468 0.0 66-67 0.0 0.0 0.0 0.9874251298164928 0.0 68-69 0.0 0.0 0.0 1.1870270674132586 0.0 70-71 0.0 0.0 0.0 1.3129521785511193 0.0 72-73 0.0 0.0 0.0 1.51027117222944 0.0 74-75 0.0 0.0 0.0 1.7550650223936044 0.0 76-77 0.0 0.0 0.0 1.9350543963273659 0.0 78-79 0.0 0.0 0.0 2.1135391026785157 0.0 80-81 0.0 0.0 0.0 2.3798652648007406 0.0 82-83 0.0 0.0 0.0 2.699373643204921 0.0 84-85 0.0 0.0 0.0 3.075281113412503 0.0 86-87 0.0 0.0 0.0 3.592679221474616 0.0 88-89 0.0 0.0 0.0 4.167410352943129 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8845 0.0 31.311335 1 GGTATCA 5925 0.0 28.38218 1 GTATAGG 395 0.0 26.457966 1 ATCAACG 10590 0.0 26.063549 3 TCAACGC 10565 0.0 25.990328 4 CAACGCA 10605 0.0 25.714447 5 TATCAAC 10795 0.0 25.565943 2 AACGCAG 10795 0.0 25.395203 6 GTACATG 6650 0.0 25.287884 1 GTGTACG 115 1.2807966E-4 24.784735 1 TACATGG 6750 0.0 24.77121 2 AGGTAAG 365 0.0 24.726866 2 GAGTACT 5480 0.0 24.617622 12-13 ACATGGG 6560 0.0 24.187782 3 TATAGGG 375 0.0 24.067482 2 GTACTTT 5640 0.0 23.880238 14-15 CGTATAG 140 1.7779132E-5 23.752037 1 ACGCAGA 11780 0.0 23.231417 7 GGTAAGG 450 0.0 23.22542 3 CGCAGAG 11805 0.0 23.101725 8 >>END_MODULE