FastQCFastQC Report
Thu 26 May 2016
SRR936655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963668
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60540.6282246582848034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.5303693803260043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.33320604191485037No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22910.23773747805260734No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18960.19674825769870952No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15840.1643719621280358No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA13060.13552385261314062No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.1314768156668064No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA12170.12628830676124972No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA11580.12016586625269282No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG11310.11736407144369222No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10830.11238310289435781No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9650.10013822187724404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG7150.031.2222255
AGGTAAG5250.030.766762
GTAAGGT7100.028.0971954
GGTAAGG6450.027.9845563
GTATCAA100100.027.480791
GTACATG69100.026.7458421
ATACCGT908.951803E-426.3862086
TACAATC2900.026.2042357
GGTATCA72150.026.0761551
TACATGG69700.025.9689482
ACATGGG68200.025.0733683
GGTGTGC8750.024.426098
GTGTGCA8300.024.0337059
TACGACG1000.001653930523.748825
GGATATG3050.023.3655571
AAGGTGT8650.023.0612416
AGGTGTG9300.022.9815377
GAGTACT64150.022.7325212-13
ACTATAC3150.022.6190993
CATGGGA44650.022.5520694
Produced by FastQC (version 0.11.5)