##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936655_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 963668 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.971777624659115 31.0 31.0 34.0 30.0 34.0 2 32.08745231760316 33.0 31.0 34.0 30.0 34.0 3 32.18710593274862 33.0 31.0 34.0 30.0 34.0 4 35.92846187691197 37.0 35.0 37.0 35.0 37.0 5 35.798698306885775 37.0 35.0 37.0 35.0 37.0 6 35.868285550625316 37.0 35.0 37.0 35.0 37.0 7 35.79455061286667 37.0 35.0 37.0 33.0 37.0 8 35.76865268951548 37.0 35.0 37.0 33.0 37.0 9 37.38174350502455 39.0 37.0 39.0 34.0 39.0 10-11 37.27977166410008 39.0 37.0 39.0 34.0 39.0 12-13 37.17015507415417 39.0 37.0 39.0 33.0 39.0 14-15 38.24875164475732 40.0 38.0 41.0 33.0 41.0 16-17 38.230262912123266 40.0 37.5 41.0 33.0 41.0 18-19 38.41717946429684 40.0 38.0 41.0 33.5 41.0 20-21 38.43571437466015 40.0 38.0 41.0 34.0 41.0 22-23 38.32931569793746 40.0 38.0 41.0 34.0 41.0 24-25 38.202046244142174 40.0 38.0 41.0 33.0 41.0 26-27 37.98437791853626 40.0 37.0 41.0 33.0 41.0 28-29 37.98362454704318 40.0 37.0 41.0 33.0 41.0 30-31 37.81020174998028 40.0 37.0 41.0 33.0 41.0 32-33 37.559894071402184 40.0 36.0 41.0 33.0 41.0 34-35 37.353070767110665 39.5 36.0 41.0 32.0 41.0 36-37 37.13535782032817 39.0 36.0 41.0 31.5 41.0 38-39 36.881159278921785 39.0 35.0 41.0 31.0 41.0 40-41 36.62247112075943 39.0 35.0 41.0 31.0 41.0 42-43 36.303212828484504 39.0 35.0 40.0 30.0 41.0 44-45 35.91408555643645 39.0 35.0 40.0 29.5 41.0 46-47 35.41485138035091 38.0 35.0 40.0 27.5 41.0 48-49 34.897202148457765 38.0 34.5 40.0 25.5 41.0 50-51 34.55944370882918 38.0 34.0 40.0 24.0 41.0 52-53 34.28134482000025 38.0 34.0 40.0 23.5 41.0 54-55 33.93670434215933 38.0 33.0 40.0 23.0 41.0 56-57 33.591669018790704 37.5 33.0 40.0 22.0 41.0 58-59 33.217455077889895 37.0 33.0 40.0 20.5 41.0 60-61 32.832643607549485 36.0 32.0 39.0 19.5 40.5 62-63 32.51731042226161 36.0 32.0 39.0 18.0 40.0 64-65 32.198586027553056 35.5 31.0 39.0 16.5 40.0 66-67 31.747005192659714 35.0 31.0 38.5 13.0 40.0 68-69 31.293685688432113 35.0 30.5 38.0 9.0 40.0 70-71 30.795298276999965 34.5 30.0 37.0 7.5 39.5 72-73 30.240712050208163 34.0 29.0 36.5 6.0 39.0 74-75 29.715040864696142 34.0 29.0 36.0 2.0 39.0 76-77 28.33972280910023 32.5 27.0 34.5 2.0 36.5 78-79 29.11693342520453 34.0 29.0 35.0 2.0 37.0 80-81 28.974286268714952 34.0 29.0 35.0 2.0 37.0 82-83 28.574247562438515 34.0 28.5 35.0 2.0 36.5 84-85 28.13953923965515 33.0 27.5 35.0 2.0 36.0 86-87 27.753868033389093 33.0 27.0 35.0 2.0 35.5 88-89 27.26706085498325 33.0 26.0 35.0 2.0 35.0 90-91 26.70887587841456 32.5 25.0 34.5 2.0 35.0 92-93 26.152903800894084 32.0 24.5 34.0 2.0 35.0 94-95 25.430400822689975 32.0 21.5 34.0 2.0 35.0 96-97 24.638810254153917 31.0 18.0 34.0 2.0 35.0 98-99 23.543311078089133 31.0 2.0 34.0 2.0 35.0 100-101 19.845967179568067 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2627959351000335 1101 2 0.37018717577513627 1101 3 0.27929695634329477 1101 4 0.1663220637628342 1101 5 0.19276953573744748 1101 6 0.15780318965985884 1101 7 0.23319629862288593 1101 8 0.11581381056971907 1101 9 0.2557410564246254 1101 10-11 0.2516772729935397 1101 12-13 0.34073163501070525 1101 14-15 0.3330249305484827 1101 16-17 0.3974659094186137 1101 18-19 0.4396232210267783 1101 20-21 0.27572316672459607 1101 22-23 0.33709597158888727 1101 24-25 0.3583838387368843 1101 26-27 0.3131712489291161 1101 28-29 0.42650490176205125 1101 30-31 0.2604958405588178 1101 32-33 0.21580506499871177 1101 34-35 0.2797597367333964 1101 36-37 0.35480260994493307 1101 38-39 0.26325706796370696 1101 40-41 0.29318351655821573 1101 42-43 0.3387851284958927 1101 44-45 0.5002938949820717 1101 46-47 0.4564079753831365 1101 48-49 0.49230128616369484 1101 50-51 0.4848128668905929 1101 52-53 0.5913361888291391 1101 54-55 0.6782030326220365 1101 56-57 0.7401158816431419 1101 58-59 0.7042651808147724 1101 60-61 0.7292522264526369 1101 62-63 0.7088819103670403 1101 64-65 0.861068479691987 1101 66-67 0.5849980643993327 1101 68-69 0.6334894931146202 1101 70-71 0.41198332919400826 1101 72-73 0.49040797296379424 1101 74-75 0.4480630513337083 1101 76-77 0.4905948155332389 1101 78-79 0.4672326332399912 1101 80-81 0.5857445541627513 1101 82-83 0.6301347722324842 1101 84-85 0.7897066149236309 1101 86-87 1.0314126173186615 1101 88-89 0.6072751128257785 1101 90-91 0.6271636333511701 1101 92-93 0.6425091126610063 1101 94-95 0.6355512569007544 1101 96-97 0.7755572806688953 1101 98-99 0.011553515519668878 1101 100-101 0.11499662030277236 1102 1 0.1839333834665986 1102 2 0.3055236909837191 1102 3 0.22347418638300098 1102 4 0.12981694003810418 1102 5 0.04451956712399152 1102 6 0.09662258222217446 1102 7 0.18527573436485767 1102 8 0.16382956352777 1102 9 0.3015638675476069 1102 10-11 0.2936035218178361 1102 12-13 0.3552867590441551 1102 14-15 0.3647809099424464 1102 16-17 0.290309159870894 1102 18-19 0.2314481067488856 1102 20-21 0.22176583801794436 1102 22-23 0.3212802404470523 1102 24-25 0.2433032730793201 1102 26-27 0.12922474600760125 1102 28-29 0.2085461899893133 1102 30-31 0.3604051283003642 1102 32-33 0.36269074800079437 1102 34-35 0.4730666333347955 1102 36-37 0.5423912383426597 1102 38-39 0.5362448817519692 1102 40-41 0.5987304965552127 1102 42-43 0.5177342246663272 1102 44-45 0.48990108980709834 1102 46-47 0.3780645880649445 1102 48-49 0.607382366354777 1102 50-51 0.7056712322793715 1102 52-53 0.8137721139706926 1102 54-55 0.5256209490906087 1102 56-57 0.6861433741978473 1102 58-59 0.5242638636089794 1102 60-61 0.4827047463290697 1102 62-63 0.33211242961368725 1102 64-65 0.1575504082459176 1102 66-67 0.2809344845221169 1102 68-69 0.26479535859085956 1102 70-71 0.18678101461645014 1102 72-73 0.11149983366104976 1102 74-75 0.4515320362825719 1102 76-77 0.381689166572702 1102 78-79 0.44431633961012196 1102 80-81 0.2852971158192972 1102 82-83 0.35572555961283214 1102 84-85 0.14925054270782212 1102 86-87 0.40503032303430686 1102 88-89 0.10452518846220471 1102 90-91 0.24490588087472176 1102 92-93 0.4631275510723718 1102 94-95 0.4590618881922133 1102 96-97 0.5125213168344267 1102 98-99 0.2325917103714481 1102 100-101 0.08621773181864967 1103 1 0.18769089308323217 1103 2 0.2800261113493647 1103 3 0.22667090592312888 1103 4 0.09307276404294385 1103 5 0.1715860623480907 1103 6 0.20391663503800572 1103 7 0.18816828741840652 1103 8 0.18360092541565365 1103 9 0.1292354541837284 1103 10-11 0.231491698763854 1103 12-13 0.2982771532179882 1103 14-15 0.3335676476353129 1103 16-17 0.2799764136202967 1103 18-19 0.24473526584470306 1103 20-21 0.2692630826909763 1103 22-23 0.21104555142081693 1103 24-25 0.2877080684287634 1103 26-27 0.188836515035554 1103 28-29 0.2483536412578431 1103 30-31 0.3762871570854216 1103 32-33 0.3169955411891934 1103 34-35 0.20377934457653168 1103 36-37 0.053507091737650114 1103 38-39 0.0490063676835959 1103 40-41 0.28891180787474013 1103 42-43 0.49657574474239397 1103 44-45 0.3974752675310924 1103 46-47 0.24656903980890377 1103 48-49 0.059860809973223184 1103 50-51 0.16973933747883052 1103 52-53 0.18471854177031588 1103 54-55 0.20675695419066642 1103 56-57 0.07227624578880665 1103 58-59 0.06147806596883498 1103 60-61 -0.07083530856137088 1103 62-63 0.12933008963875636 1103 64-65 -0.0974224166665536 1103 66-67 -0.1842071176734983 1103 68-69 -0.12421358811787542 1103 70-71 -0.21403207696845783 1103 72-73 -0.044185864571215205 1103 74-75 0.09200562836452164 1103 76-77 -0.07631694917263943 1103 78-79 -0.26343963566757367 1103 80-81 -0.010911608302237141 1103 82-83 -0.051762986871157324 1103 84-85 0.06223812752867275 1103 86-87 0.12232648286488157 1103 88-89 0.04330452459048573 1103 90-91 0.06830508993380491 1103 92-93 0.06311485495792368 1103 94-95 0.03054425409963457 1103 96-97 0.39744103417029564 1103 98-99 0.6018371289650446 1103 100-101 0.2665722505548729 1104 1 0.26331138291484635 1104 2 0.3116000234420824 1104 3 0.23058245195417726 1104 4 0.21193346817589997 1104 5 0.10100056048935357 1104 6 0.15068844785235314 1104 7 0.17110618376653264 1104 8 0.17589263763957774 1104 9 0.25036217624801793 1104 10-11 0.2299951428924274 1104 12-13 0.21548822909815613 1104 14-15 0.3319180193825275 1104 16-17 0.29414517592075384 1104 18-19 0.1798708442396375 1104 20-21 0.3151041607578904 1104 22-23 0.0806060150114618 1104 24-25 0.1709972112234297 1104 26-27 0.2074047415826854 1104 28-29 0.33137900753819594 1104 30-31 0.3433738616557278 1104 32-33 0.6393987775766803 1104 34-35 0.4785005341676083 1104 36-37 0.3222283906661474 1104 38-39 0.5348991076792231 1104 40-41 0.41794635849796435 1104 42-43 0.7010736126627961 1104 44-45 0.908721987408633 1104 46-47 0.8138689523389786 1104 48-49 0.535212056419752 1104 50-51 0.5466541089636365 1104 52-53 0.6474481503423632 1104 54-55 0.38808895725210846 1104 56-57 0.566255854360854 1104 58-59 0.49287430476174876 1104 60-61 0.46781136758119857 1104 62-63 0.456243494405868 1104 64-65 0.28676329293326575 1104 66-67 0.40845770848000384 1104 68-69 0.3530223620242623 1104 70-71 0.2762286927669493 1104 72-73 0.4358709908847196 1104 74-75 0.6791940167310173 1104 76-77 0.6729805579342916 1104 78-79 0.40917134180892134 1104 80-81 0.18826972780097861 1104 82-83 0.30780677039978244 1104 84-85 0.11810544657524957 1104 86-87 0.3171971913713314 1104 88-89 0.20700723615838257 1104 90-91 0.3338774713027064 1104 92-93 0.20418608828396856 1104 94-95 0.348620462278074 1104 96-97 0.4008878961864788 1104 98-99 0.32722391007153817 1104 100-101 0.623403105573459 1105 1 0.1518812243881662 1105 2 0.24506724274445446 1105 3 0.23302373441084256 1105 4 0.052854147860834644 1105 5 0.11454583246302974 1105 6 0.22192148065441586 1105 7 0.14412388804946374 1105 8 0.08320651477783514 1105 9 0.21609876272553663 1105 10-11 0.19326908213098903 1105 12-13 0.09792922669105764 1105 14-15 0.14317013805677448 1105 16-17 0.16315057408908018 1105 18-19 0.24865120003447316 1105 20-21 0.1795360711967291 1105 22-23 0.12513164562862045 1105 24-25 0.14167048672941718 1105 26-27 0.19400047717213909 1105 28-29 0.29071897819722636 1105 30-31 0.25203372007843683 1105 32-33 0.2177505558387267 1105 34-35 -0.004266632434962503 1105 36-37 0.11486893532566711 1105 38-39 0.05955121780755235 1105 40-41 0.19237698704986173 1105 42-43 0.285220944201491 1105 44-45 0.20051363805171007 1105 46-47 0.26220178716071985 1105 48-49 0.18995575024818123 1105 50-51 0.18186042154464133 1105 52-53 0.09368011490531813 1105 54-55 0.04042412795671879 1105 56-57 0.04045716758280804 1105 58-59 0.11333179596658027 1105 60-61 0.24698782643975647 1105 62-63 0.2279721901571179 1105 64-65 0.17997926239587514 1105 66-67 0.16125293416332553 1105 68-69 0.2265414265316643 1105 70-71 0.18530587660377762 1105 72-73 0.21608008088742636 1105 74-75 0.3269870347201653 1105 76-77 0.056213476790848205 1105 78-79 0.12730861765674462 1105 80-81 0.4050239900750441 1105 82-83 0.24321391193434394 1105 84-85 0.3271313549345791 1105 86-87 0.3489196201197835 1105 88-89 0.2163521919332858 1105 90-91 0.22657926167146414 1105 92-93 0.27656284909810935 1105 94-95 0.11392548015327364 1105 96-97 0.47419616150859056 1105 98-99 0.8051825763393978 1105 100-101 0.8435358520874416 1106 1 0.020683521272228944 1106 2 0.208066039895634 1106 3 0.1838911293418377 1106 4 0.11814950132468027 1106 5 0.05786707175784045 1106 6 0.0680708895541855 1106 7 7.620075411836069E-4 1106 8 0.06690742166684771 1106 9 0.10362092693570446 1106 10-11 0.13009690431912446 1106 12-13 0.11589559280940165 1106 14-15 0.18521970067209992 1106 16-17 0.18801086991778249 1106 18-19 0.17331550033370746 1106 20-21 0.039114451382168625 1106 22-23 0.006924381994963369 1106 24-25 0.06107928817414887 1106 26-27 -0.18486838608657052 1106 28-29 0.055870120903598774 1106 30-31 -0.07961595266146304 1106 32-33 0.002183987825326028 1106 34-35 0.007029986150527634 1106 36-37 0.012084684451387773 1106 38-39 0.28156498993489265 1106 40-41 -0.005509014346365859 1106 42-43 -0.030941804193354017 1106 44-45 -0.17325606392364534 1106 46-47 -0.08610783333556071 1106 48-49 -0.3278860702159818 1106 50-51 -0.3841802393339293 1106 52-53 -0.2165690091782082 1106 54-55 -0.23123649169068017 1106 56-57 -0.1252206721400455 1106 58-59 -0.10587084964531357 1106 60-61 -0.2319052904463419 1106 62-63 -0.2752653413480175 1106 64-65 -0.4027610041973766 1106 66-67 -0.7727654002679749 1106 68-69 -0.5778189823713866 1106 70-71 -0.49441186361365297 1106 72-73 -0.3090728972285923 1106 74-75 -0.5403718435823421 1106 76-77 -0.4896170296838882 1106 78-79 -0.5473348704081751 1106 80-81 -0.6210519368887617 1106 82-83 -0.4500137542439724 1106 84-85 -0.3538990309275256 1106 86-87 -0.31492412096946865 1106 88-89 -0.21578255335446528 1106 90-91 -0.05919893562851897 1106 92-93 -0.21421651172048328 1106 94-95 -0.1303518109132007 1106 96-97 -0.06683147123905187 1106 98-99 0.6003108747507859 1106 100-101 0.6449618998277558 1107 1 -0.012980031852400487 1107 2 0.09418485565517187 1107 3 -0.006219270349397732 1107 4 0.05139124294965569 1107 5 -0.013388685126656696 1107 6 0.019303345277663198 1107 7 -0.08866375853128972 1107 8 -0.15872428749779033 1107 9 -0.21563234141935794 1107 10-11 -0.06007470052313835 1107 12-13 -0.10575706147798769 1107 14-15 0.04840350622117029 1107 16-17 0.038551131386192594 1107 18-19 -0.06762361710241294 1107 20-21 -0.13669230451440484 1107 22-23 -0.1480106973413129 1107 24-25 -0.029373284314353043 1107 26-27 -0.017276081518211583 1107 28-29 0.061153205528000854 1107 30-31 0.0032702230390881937 1107 32-33 -0.20058219697678226 1107 34-35 -0.08494119414400814 1107 36-37 -0.01750944619065109 1107 38-39 0.17683613595454517 1107 40-41 0.26022791007319057 1107 42-43 0.09587634882240792 1107 44-45 0.12462544578421131 1107 46-47 0.2490472533165331 1107 48-49 0.3957899543582428 1107 50-51 0.27422661218669475 1107 52-53 0.19195017179605856 1107 54-55 0.09395367631385909 1107 56-57 0.3606737007303735 1107 58-59 0.351921718177195 1107 60-61 0.20963730977889128 1107 62-63 0.06558974501605519 1107 64-65 0.15796602658953418 1107 66-67 -0.23801851597019308 1107 68-69 0.09558241108988952 1107 70-71 -0.0706299181251211 1107 72-73 0.2876521231491225 1107 74-75 0.25761515500345666 1107 76-77 -0.05238399215145151 1107 78-79 0.017274367105251542 1107 80-81 0.2150205069681128 1107 82-83 0.3978928855788375 1107 84-85 0.10903080924782671 1107 86-87 0.29302736422752673 1107 88-89 0.33137889575309387 1107 90-91 0.11853038207085831 1107 92-93 -0.009083847755483987 1107 94-95 0.01829677935215912 1107 96-97 0.24308621143311981 1107 98-99 0.07397393823420728 1107 100-101 -0.29357084708822256 1108 1 0.11142058760005469 1108 2 0.23670890227911912 1108 3 -0.0373537378646418 1108 4 0.10472381466111358 1108 5 0.15585797563743853 1108 6 0.06926494684152118 1108 7 0.07421225260108599 1108 8 0.1795841820547892 1108 9 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-0.7501909505226365 2316 90-91 -0.9977016973965149 2316 92-93 -0.9947188554493174 2316 94-95 -1.0403267866243517 2316 96-97 -0.7299910925265358 2316 98-99 0.09049663122748797 2316 100-101 -0.27454765894739097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 15.0 5 32.0 6 85.0 7 207.0 8 429.0 9 854.0 10 1408.0 11 2395.0 12 3391.0 13 4761.0 14 6065.0 15 8343.0 16 11051.0 17 14681.0 18 12630.0 19 7868.0 20 6649.0 21 6817.0 22 7331.0 23 8008.0 24 9197.0 25 10662.0 26 13033.0 27 15764.0 28 19204.0 29 23484.0 30 29007.0 31 35893.0 32 44855.0 33 58457.0 34 77385.0 35 106389.0 36 147237.0 37 177704.0 38 89053.0 39 3318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32305195142151 20.5505866822202 15.088204708817566 21.03815665754073 2 15.686939917080487 22.12425473621943 39.117877509508766 23.07092783719132 3 18.974535129648963 27.06262544079402 30.23800282689326 23.724836602663753 4 12.693415242848916 17.76623619203769 37.612448309319106 31.927900255794285 5 12.483110737523738 39.54204414557454 33.54077809947801 14.434067017423699 6 30.157481622301457 37.97002702175438 17.098627328083946 14.773864027860217 7 27.930585764332793 34.760663467764694 20.54305235019675 16.765698417705757 8 27.649683029057137 33.340839493054034 19.39858019432306 19.610897283565766 9 27.983791047718864 16.26052622021138 20.299066584342068 35.456616147727686 10-11 24.729938111465774 27.49997924596438 26.472083746684543 21.297998895885307 12-13 26.46772539920387 25.327913762831184 27.160131912650414 21.044228925314528 14-15 23.279978000653752 26.316199094083526 26.521405260126702 23.882417645136016 16-17 21.208585028916673 29.426668506928483 27.31195862316592 22.052787840988927 18-19 21.132125986866683 27.149366578945184 31.181822144001593 20.53668529018654 20-21 22.257481223976235 25.991205427211288 31.1817674756463 20.569545873166177 22-23 21.74774338191001 25.99688377346704 31.346198447490337 20.909174397132617 24-25 21.584149271713056 26.411602366173742 31.340387302440526 20.663861059672676 26-27 21.157775455629384 26.494888794573228 31.357771927439664 20.989563822357724 28-29 20.80198823764421 26.675072055454812 31.651632954312557 20.87130675258842 30-31 21.142928299772223 26.53136722823803 31.01269632081688 21.313008151172866 32-33 20.95721635175418 26.692887033630736 30.995431325901347 21.35446528871374 34-35 21.579524387921715 26.475683680039765 31.099911950592713 20.844879981445803 36-37 22.174026289382446 25.93827277076227 30.7789373626978 21.10876357715748 38-39 21.42245975430995 26.683910804394923 30.84170244782005 21.051926993475075 40-41 21.304121336393862 26.688340797868143 31.137590954561112 20.86994691117688 42-43 21.460488534821 26.846533569516957 30.07678043121152 21.616197464450526 44-45 21.40248686209559 26.78160776402414 30.1587029795854 21.65720239429487 46-47 22.283504277406742 27.39869955212791 29.072616295238607 21.24517987522674 48-49 22.1019850547553 27.65274980257375 28.37884254776885 21.866422594902097 50-51 22.061371262359785 27.76935214149811 28.575394109641422 21.59388248650068 52-53 22.358859147110252 27.72524684407963 28.427409940176307 21.48848406863381 54-55 21.8109462029175 27.986987212913167 28.40767173324824 21.79439485092109 56-57 22.286307323084905 28.16272945449828 27.66924823247065 21.881714989946165 58-59 22.090714329817366 27.351226039704184 28.03570585424295 22.522353776235494 60-61 21.723670597220767 27.947215110927548 28.544788703747326 21.78432558810436 62-63 21.763834517611105 27.967322753958186 28.86187403850394 21.406968689926764 64-65 21.571963447006254 27.980878791086315 28.551163191309637 21.895994570597797 66-67 21.56962955314657 28.01136914919725 28.193331572685608 22.22566972497057 68-69 21.988695089098552 27.797638566619487 28.222428843607446 21.99123750067452 70-71 22.04133223263481 27.924759542168914 28.013123287401136 22.020784937795142 72-73 21.87403037425608 27.91484415941555 27.890665130831376 22.320460335496996 74-75 21.784785482863327 28.673181777614502 27.450223431568503 22.091809307953667 76-77 21.886909342034876 27.99523276091528 28.049349329976685 22.068508567073156 78-79 21.913269078597466 28.014943766296103 27.735111364786157 22.336675790320278 80-81 22.19849872645472 28.518386112778675 27.14937033576039 22.133744825006215 82-83 22.26449829007632 28.52435999146984 27.19818977947666 22.012951938977178 84-85 21.746198192607615 28.635292664842705 27.213983034358304 22.404526108191376 86-87 21.813331008869284 28.699451986846025 26.891091595298356 22.596125408986335 88-89 21.575653426396073 28.592042139737313 26.87471916700133 22.957585266865284 90-91 21.45585380648288 28.428460164207227 27.322710316889953 22.792975712419942 92-93 21.873265058128013 28.273935841784713 27.24576064521418 22.60703845487309 94-95 21.411456690298696 28.053346394593966 27.430567085623203 23.104629829484136 96-97 21.9498426583858 27.719000588896066 27.266769918047462 23.06438683467067 98-99 21.315676418336682 28.20837968420528 26.88769114546149 23.588252751996546 100-101 20.984111978357298 28.42908295071709 26.966934588836928 23.619870482088682 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 463.0 1 337.5 2 227.5 3 252.0 4 258.5 5 273.5 6 398.0 7 741.5 8 1147.0 9 1374.0 10 1334.0 11 1211.5 12 1252.0 13 1384.0 14 1518.0 15 1636.5 16 1716.0 17 1819.5 18 2021.0 19 2252.0 20 2404.0 21 2620.0 22 3016.0 23 3553.5 24 4081.5 25 4761.5 26 5967.0 27 7396.5 28 8530.5 29 10284.5 30 12776.5 31 15188.5 32 19147.0 33 21980.5 34 24042.5 35 26840.5 36 30986.5 37 34127.5 38 34257.0 39 34018.5 40 32338.5 41 30182.0 42 27934.5 43 28360.5 44 29526.5 45 30275.0 46 30125.5 47 30011.5 48 34119.0 49 33236.0 50 28468.5 51 26354.5 52 25577.0 53 27042.0 54 28500.5 55 27125.5 56 23883.5 57 21806.0 58 21575.5 59 19919.5 60 16570.5 61 14134.0 62 12098.5 63 8884.5 64 5873.0 65 4509.5 66 3615.5 67 2995.5 68 2414.0 69 1945.5 70 1571.5 71 1146.5 72 953.0 73 869.5 74 664.5 75 507.0 76 402.0 77 263.0 78 163.5 79 111.5 80 76.5 81 57.5 82 45.5 83 32.0 84 19.5 85 9.0 86 4.5 87 2.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011829800304669243 2 0.008612924783224098 3 0.006848831755334826 4 3.113105343334011E-4 5 0.0039432667682230815 6 0.0 7 4.150807124445348E-4 8 0.0015565526716670058 9 3.113105343334011E-4 10-11 0.0 12-13 0.0 14-15 3.113105343334011E-4 16-17 0.0038394965901119476 18-19 0.0012452421373336045 20-21 5.707359796112354E-4 22-23 2.594254452778343E-4 24-25 2.594254452778343E-4 26-27 1.037701781111337E-4 28-29 5.188508905556685E-5 30-31 3.113105343334011E-4 32-33 0.004202692213500915 34-35 4.6696580150010165E-4 36-37 9.339316030002033E-4 38-39 0.005759244885167921 40-41 0.0 42-43 0.0011414719592224708 44-45 0.004565887836889883 46-47 0.0 48-49 0.0017640930278892731 50-51 8.820465139446366E-4 52-53 3.113105343334011E-4 54-55 5.188508905556685E-5 56-57 0.010947753790724605 58-59 0.0017122079388337064 60-61 0.002179173740333808 62-63 5.188508905556685E-5 64-65 0.0026980246308894763 66-67 7.782763358335029E-4 68-69 0.0016603228497781392 70-71 0.003891381679167514 72-73 0.0023867140965560755 74-75 0.00643375104289029 76-77 7.782763358335029E-4 78-79 0.005759244885167921 80-81 0.0020235184731671072 82-83 0.0029574500761673107 84-85 0.0015046675826114388 86-87 5.188508905556686E-4 88-89 0.0017640930278892731 90-91 0.00710825720061266 92-93 0.0019716333841115407 94-95 4.150807124445348E-4 96-97 5.188508905556685E-5 98-99 4.150807124445348E-4 100-101 0.0018159781169448399 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 963668.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.590305864818575 #Duplication Level Percentage of deduplicated Percentage of total 1 74.72681161632558 32.5736457465831 2 12.921788790529318 11.26529451399514 3 4.625189106060143 6.048402234473653 4 2.2196837476565623 3.870267739340653 5 1.2715809977237909 2.771430231133561 6 0.7841477267098175 2.0508743550289865 7 0.5569946478140712 1.6995696944297585 8 0.38885742837078635 1.3560331392391485 9 0.2992597872925169 1.174034309500922 >10 1.85278050124388 15.609176250609028 >50 0.21919470454780987 6.591616128366503 >100 0.12392139434876462 10.12348178970725 >500 0.006924316827614359 2.023842898047917 >1k 0.002387695457798055 1.6802735791249972 >5k 4.77539091559611E-4 1.1620573904193876 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6054 0.6282246582848034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5111 0.5303693803260043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3211 0.33320604191485037 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2291 0.23773747805260734 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1896 0.19674825769870952 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1584 0.1643719621280358 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1306 0.13552385261314062 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.1314768156668064 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1217 0.12628830676124972 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1158 0.12016586625269282 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1131 0.11736407144369222 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1083 0.11238310289435781 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 965 0.10013822187724404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.188508905556686E-5 0.0 24-25 0.0 0.0 0.0 2.594254452778343E-4 0.0 26-27 0.0 0.0 0.0 0.001037701781111337 0.0 28-29 0.0 0.0 0.0 0.003164990432389578 0.0 30-31 0.0 0.0 0.0 0.005499819439890087 0.0 32-33 0.0 0.0 0.0 0.008197844070779564 0.0 34-35 0.0 0.0 0.0 0.01089586870166904 0.0 36-37 0.0 0.0 0.0 0.014060859134058617 0.0 38-39 0.0 0.0 0.0 0.0183154364366151 0.0 40-41 0.0 0.0 0.0 0.025994429616838996 0.0 42-43 0.0 0.0 0.0 0.03714972376378587 0.0 44-45 0.0 0.0 0.0 0.056191551447178904 0.0 46-47 0.0 0.0 0.0 0.07798328885051697 0.0 48-49 0.0 0.0 0.0 0.11902439429347036 0.0 50-51 0.0 0.0 0.0 0.1598060742911459 0.0 52-53 0.0 0.0 0.0 0.2038046298102666 0.0 54-55 0.0 0.0 0.0 0.2763918693990046 0.0 56-57 0.0 0.0 0.0 0.3530261459340769 0.0 58-59 0.0 0.0 0.0 0.449324871221209 0.0 60-61 0.0 0.0 0.0 0.5640428031230673 0.0 62-63 0.0 0.0 0.0 0.714768986829489 0.0 64-65 0.0 0.0 0.0 0.8301614248890696 0.0 66-67 0.0 0.0 0.0 0.9883071763304375 0.0 68-69 0.0 0.0 0.0 1.1866638717898694 0.0 70-71 0.0 0.0 0.0 1.3124852127496192 0.0 72-73 0.0 0.0 0.0 1.5104268274966066 0.0 74-75 0.0 0.0 0.0 1.7548055969483265 0.0 76-77 0.0 0.0 0.0 1.9350025112383102 0.0 78-79 0.0 0.0 0.0 2.11302025178796 0.0 80-81 0.0 0.0 0.0 2.3760257682106287 0.0 82-83 0.0 0.0 0.0 2.695067180813309 0.0 84-85 0.0 0.0 0.0 3.0684322816571683 0.0 86-87 0.0 0.0 0.0 3.5810050764371133 0.0 88-89 0.0 0.0 0.0 4.150340158643848 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 715 0.0 31.222225 5 AGGTAAG 525 0.0 30.76676 2 GTAAGGT 710 0.0 28.097195 4 GGTAAGG 645 0.0 27.984556 3 GTATCAA 10010 0.0 27.48079 1 GTACATG 6910 0.0 26.745842 1 ATACCGT 90 8.951803E-4 26.386208 6 TACAATC 290 0.0 26.204235 7 GGTATCA 7215 0.0 26.076155 1 TACATGG 6970 0.0 25.968948 2 ACATGGG 6820 0.0 25.073368 3 GGTGTGC 875 0.0 24.42609 8 GTGTGCA 830 0.0 24.033705 9 TACGACG 100 0.0016539305 23.74882 5 GGATATG 305 0.0 23.365557 1 AAGGTGT 865 0.0 23.061241 6 AGGTGTG 930 0.0 22.981537 7 GAGTACT 6415 0.0 22.73252 12-13 ACTATAC 315 0.0 22.619099 3 CATGGGA 4465 0.0 22.552069 4 >>END_MODULE