FastQCFastQC Report
Thu 26 May 2016
SRR936654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094796
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70110.6403932787478215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52600.4804548061922038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.2954888399299961No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16580.15144373929024219No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13200.12057040763758728No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.11499859334524423No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA11690.10677788373359057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.032.379951
GTATCAA93850.030.5817361
GTACATG75500.027.3780021
TACATGG76650.026.5320762
ACATGGG76100.026.0370033
CCTATAC3150.025.643543
CATGGGA47600.025.554814
GTATAGG5250.025.343011
ATCAACG115250.024.7783953
TCAACGC115900.024.5984334
CAACGCA117300.024.348695
TATCAAC117850.024.2720532
AACGCAG119050.023.9907726
GAGTACT63250.023.81160712-13
AGGTAAG3650.023.4325352
GTACTTT66550.022.556914-15
GGTAAGG4100.022.0195963
ACGCAGA131850.021.6607597
GGGTATG2205.6132194E-821.6001437
CGCAGAG132050.021.5559778
Produced by FastQC (version 0.11.5)