##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936654_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1094796 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.493900233468153 31.0 31.0 34.0 30.0 34.0 2 31.542358576392314 31.0 31.0 34.0 30.0 34.0 3 31.566063449263606 33.0 31.0 34.0 28.0 34.0 4 35.24268082820909 37.0 35.0 37.0 33.0 37.0 5 35.18497966744489 37.0 35.0 37.0 33.0 37.0 6 35.30159317352274 37.0 35.0 37.0 33.0 37.0 7 35.146866630860906 37.0 35.0 37.0 32.0 37.0 8 35.13073485836631 37.0 35.0 37.0 32.0 37.0 9 36.647291367524176 39.0 37.0 39.0 32.0 39.0 10-11 36.53230921559816 39.0 37.0 39.0 32.0 39.0 12-13 36.45148593893292 39.0 37.0 39.0 32.0 39.0 14-15 37.50924601478266 40.0 37.0 41.0 32.0 41.0 16-17 37.46929062583349 40.0 37.0 41.0 32.0 41.0 18-19 37.49382122331466 40.0 37.0 41.0 32.0 41.0 20-21 37.423767989652866 40.0 37.0 41.0 32.0 41.0 22-23 37.327483385032465 39.5 37.0 41.0 32.0 41.0 24-25 37.180594832279255 39.0 36.0 41.0 31.0 41.0 26-27 36.95995920701208 39.0 36.0 41.0 31.0 41.0 28-29 37.043803594459604 39.0 36.0 41.0 31.0 41.0 30-31 36.93431516008462 39.0 36.0 41.0 31.0 41.0 32-33 36.72589733612472 39.0 35.0 41.0 30.0 41.0 34-35 36.537689670038986 39.0 35.0 41.0 30.0 41.0 36-37 36.271210344210246 39.0 35.0 40.0 30.0 41.0 38-39 36.06294597349643 38.0 35.0 40.0 30.0 41.0 40-41 35.87589834087812 38.0 35.0 40.0 29.0 41.0 42-43 35.56098853119668 38.0 35.0 40.0 27.5 41.0 44-45 35.20631880277239 38.0 34.5 40.0 26.5 41.0 46-47 34.741101538551476 38.0 34.0 40.0 24.5 41.0 48-49 34.379391229050896 38.0 34.0 40.0 23.0 41.0 50-51 33.6241445894943 37.5 33.0 39.5 22.5 40.5 52-53 33.79199184140242 38.0 33.0 39.5 23.0 40.5 54-55 34.1323634722816 38.0 34.0 40.0 22.5 41.0 56-57 34.04532488244385 38.0 34.0 40.0 22.0 41.0 58-59 33.81218190420864 38.0 33.5 40.0 21.0 41.0 60-61 33.49021781226822 37.0 33.0 40.0 19.5 41.0 62-63 33.168982623246706 37.0 33.0 40.0 18.5 41.0 64-65 32.85178928311758 36.0 32.5 39.5 17.5 41.0 66-67 32.47977020376399 36.0 32.0 39.0 14.5 41.0 68-69 32.09902484115762 35.0 32.0 39.0 12.0 40.5 70-71 31.69500208257977 35.0 31.0 38.0 8.0 40.0 72-73 31.29555323548862 35.0 31.0 37.5 7.0 40.0 74-75 30.885132024596363 35.0 31.0 37.0 6.0 39.0 76-77 30.488415193332823 35.0 30.0 36.5 2.0 39.0 78-79 30.053702242244217 34.5 30.0 36.0 2.0 38.5 80-81 29.66679089072302 34.0 29.5 35.5 2.0 37.0 82-83 29.273886641894926 34.0 29.0 35.0 2.0 37.0 84-85 28.89328057464587 34.0 29.0 35.0 2.0 36.5 86-87 28.58708060679798 34.0 29.0 35.0 2.0 36.0 88-89 28.216475946203676 34.0 28.5 35.0 2.0 36.0 90-91 27.869673893583826 34.0 27.0 35.0 2.0 35.0 92-93 27.533859732772132 34.0 27.0 35.0 2.0 35.0 94-95 27.125015071300954 34.0 26.0 35.0 2.0 35.0 96-97 26.57922023829097 33.0 25.0 35.0 2.0 35.0 98-99 25.956824833119597 33.0 24.5 35.0 2.0 35.0 100-101 23.959436735245653 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22044156504812662 1101 2 0.3347108805507588 1101 3 0.32078825254243526 1101 4 0.4105442412604887 1101 5 0.47288923481982437 1101 6 0.4016311229362799 1101 7 0.293411929329757 1101 8 0.35790681937344004 1101 9 0.645620674813955 1101 10-11 0.34878927654546743 1101 12-13 0.6279940366532131 1101 14-15 0.5364813235559254 1101 16-17 0.5507800236283416 1101 18-19 0.6110544128308035 1101 20-21 0.6544162282826491 1101 22-23 0.4999605316601361 1101 24-25 0.4035708063224419 1101 26-27 0.5373729533308946 1101 28-29 0.6105161621735746 1101 30-31 0.46892637724225494 1101 32-33 0.49863092017582034 1101 34-35 0.4599625559307867 1101 36-37 0.6095137870193241 1101 38-39 0.6556265083570025 1101 40-41 0.48984138643167796 1101 42-43 0.6485285774579097 1101 44-45 0.5983233876329521 1101 46-47 0.6185266048460107 1101 48-49 0.6293278028229636 1101 50-51 0.8469595890593311 1101 52-53 0.9485954843574618 1101 54-55 1.0482335952815731 1101 56-57 0.8497862961634013 1101 58-59 0.8463388860002894 1101 60-61 0.8695644778334994 1101 62-63 0.9139083387349061 1101 64-65 1.0314325903607582 1101 66-67 0.9876314342372012 1101 68-69 0.927481532513589 1101 70-71 0.7870109405025438 1101 72-73 0.8325795433715477 1101 74-75 0.7988071988597163 1101 76-77 0.955281300295173 1101 78-79 0.845631491199125 1101 80-81 0.7983880877344269 1101 82-83 0.8823950277267194 1101 84-85 0.9395293584848439 1101 86-87 0.6630395157771751 1101 88-89 0.4713422426859495 1101 90-91 0.4697423593328658 1101 92-93 0.19510195930126528 1101 94-95 0.16664196483372606 1101 96-97 0.23874887226872588 1101 98-99 0.22527745096378382 1101 100-101 0.35353640171626566 1102 1 -0.04386981988320571 1102 2 0.04417051346052858 1102 3 0.018683259601864677 1102 4 0.10759211701456195 1102 5 0.060304200806221786 1102 6 0.2004631085607187 1102 7 -0.07747114087945306 1102 8 0.06632037347432629 1102 9 0.21522663040374113 1102 10-11 0.24016073078754374 1102 12-13 0.33941105629125445 1102 14-15 0.0463548956262656 1102 16-17 -0.04890551096284668 1102 18-19 0.06913745736937216 1102 20-21 -0.04946324803618296 1102 22-23 -0.5008641378302983 1102 24-25 -0.14759399922241556 1102 26-27 -0.0552242742393787 1102 28-29 -0.1940885098839189 1102 30-31 -0.200974790772122 1102 32-33 -0.13827384172893886 1102 34-35 -0.20832136140973034 1102 36-37 -0.3683549076283512 1102 38-39 -0.43956666325127003 1102 40-41 -0.35917029371208287 1102 42-43 -0.2362519385220665 1102 44-45 -0.2140402370622141 1102 46-47 -0.12868171244316784 1102 48-49 0.011637519162334797 1102 50-51 -0.22128261348206735 1102 52-53 -0.2845260661495317 1102 54-55 -0.18930651510220287 1102 56-57 -0.2306013305854151 1102 58-59 -0.2689640276179901 1102 60-61 -0.7006286937747674 1102 62-63 -0.3958850125678808 1102 64-65 -0.30901151180840003 1102 66-67 -0.5198676031135854 1102 68-69 -0.39645313569331364 1102 70-71 -0.23960951900201266 1102 72-73 -0.44069410571072254 1102 74-75 -0.6585602764322864 1102 76-77 -0.4209669348479359 1102 78-79 -0.5995905598854598 1102 80-81 -0.5657756909846121 1102 82-83 -0.5338641174407819 1102 84-85 -0.6722374236997268 1102 86-87 -0.5893318101116698 1102 88-89 -0.7384607351089407 1102 90-91 -0.8616111254572765 1102 92-93 -0.8125511019232228 1102 94-95 -0.6749797782031095 1102 96-97 -0.7691544777505257 1102 98-99 -0.8207821049340502 1102 100-101 -0.6582624687753267 1103 1 0.08371390591480576 1103 2 0.3002406338163617 1103 3 0.2698344893245235 1103 4 0.19167113323644003 1103 5 0.16451011394070747 1103 6 0.10119570929332156 1103 7 -0.026228336065223345 1103 8 -0.1093075700107562 1103 9 0.17490742579882124 1103 10-11 0.18850910860020775 1103 12-13 0.33477016147178773 1103 14-15 0.41253098144520806 1103 16-17 0.3979122546049112 1103 18-19 0.30938143957763486 1103 20-21 0.17714585714435316 1103 22-23 0.32075412485939125 1103 24-25 0.16083417974147807 1103 26-27 0.08631811194586447 1103 28-29 0.20458365128823885 1103 30-31 0.19076055721323115 1103 32-33 0.21497707402198074 1103 34-35 0.21665574481737337 1103 36-37 0.4299574942554756 1103 38-39 0.25049743931283786 1103 40-41 0.032702416910630916 1103 42-43 0.24004319491591986 1103 44-45 0.40886661798750623 1103 46-47 0.4912653048252764 1103 48-49 0.5743762685082174 1103 50-51 0.15044030645513828 1103 52-53 0.5716192766029522 1103 54-55 0.5349314493143353 1103 56-57 0.5353360058876362 1103 58-59 0.5602876730622768 1103 60-61 0.846646947250882 1103 62-63 0.41370747827460974 1103 64-65 0.39653859284884163 1103 66-67 0.46493420222393667 1103 68-69 0.5803881777551396 1103 70-71 0.5711701774919682 1103 72-73 0.5695819277795984 1103 74-75 0.4769250715530582 1103 76-77 0.3754943419704837 1103 78-79 0.514276525410196 1103 80-81 0.4610656224638454 1103 82-83 0.6122701054791548 1103 84-85 0.4078711581945704 1103 86-87 0.4511770857543702 1103 88-89 0.4625367792814288 1103 90-91 0.41330056999013465 1103 92-93 0.3911871241364331 1103 94-95 0.16432882985549924 1103 96-97 0.41132040451007157 1103 98-99 0.05878356748876712 1103 100-101 0.05155241871813132 1104 1 0.2863629865061128 1104 2 0.2803122740163886 1104 3 0.4875023421564286 1104 4 0.4495778128620884 1104 5 0.3343626401591351 1104 6 0.3298564921623779 1104 7 0.47100285642274287 1104 8 0.38023053097527537 1104 9 0.5375251931954708 1104 10-11 0.5732601902574928 1104 12-13 0.5406803799979158 1104 14-15 0.6296217544382685 1104 16-17 0.4451098412550678 1104 18-19 0.5972644285572741 1104 20-21 0.5307850852486311 1104 22-23 0.33848683793804213 1104 24-25 0.40650510118808114 1104 26-27 0.41389273062247867 1104 28-29 0.3138966813395001 1104 30-31 0.4412675840665443 1104 32-33 0.48051903918947403 1104 34-35 0.4387712274089637 1104 36-37 0.49109660478831074 1104 38-39 0.474477898938801 1104 40-41 0.36900034686939875 1104 42-43 0.3852596047543031 1104 44-45 0.35788568023766487 1104 46-47 0.7626324451742263 1104 48-49 0.6936040004766824 1104 50-51 0.42206071529023603 1104 52-53 0.4611045684152728 1104 54-55 0.7398942379522282 1104 56-57 0.5536825398814642 1104 58-59 0.6900512760783784 1104 60-61 0.6048362882237441 1104 62-63 0.3537141994421802 1104 64-65 0.5921554314310882 1104 66-67 0.45630200881107186 1104 68-69 0.6674914721070095 1104 70-71 0.6382015769056828 1104 72-73 0.33194750442833865 1104 74-75 0.2251840344161593 1104 76-77 0.24109940381306316 1104 78-79 0.3851634843855969 1104 80-81 0.40164354489804666 1104 82-83 0.2311377748996044 1104 84-85 0.4129159529148261 1104 86-87 0.39857856088408994 1104 88-89 0.4808362846975527 1104 90-91 0.45184195905755686 1104 92-93 0.2137233165460195 1104 94-95 0.44613631445364277 1104 96-97 0.7716778608910637 1104 98-99 0.7594113990010491 1104 100-101 0.5666383046581558 1105 1 0.1406354232620899 1105 2 0.17790190994925226 1105 3 0.11885555559449656 1105 4 0.3115965135329972 1105 5 0.3264450676333013 1105 6 0.12211002060064402 1105 7 0.3447847483758082 1105 8 -0.05036560222599462 1105 9 0.3472885381362616 1105 10-11 0.29408789117135115 1105 12-13 0.31442219611836464 1105 14-15 0.3229523468338229 1105 16-17 0.38795246366805003 1105 18-19 0.20925717337804173 1105 20-21 -0.00841966903252711 1105 22-23 -0.12471970625854567 1105 24-25 -0.11848934425999147 1105 26-27 -0.02765988603868408 1105 28-29 0.047999242247499296 1105 30-31 -0.1339026343986589 1105 32-33 -0.03185878662392838 1105 34-35 -0.003985740698567497 1105 36-37 0.04477226463793471 1105 38-39 -0.08661386641200863 1105 40-41 -0.22814513255590896 1105 42-43 0.01726379091380892 1105 44-45 -0.06284307697546154 1105 46-47 0.1106983190454045 1105 48-49 0.27205400016521253 1105 50-51 0.27211221169691413 1105 52-53 0.3104298201351128 1105 54-55 0.3305045534300177 1105 56-57 0.7213459111933958 1105 58-59 0.4769023538691002 1105 60-61 0.5147487235677133 1105 62-63 0.042594920890223875 1105 64-65 0.26740621685511456 1105 66-67 -0.04036785561633138 1105 68-69 0.27946552871568286 1105 70-71 0.3806100098575662 1105 72-73 0.5004757246668419 1105 74-75 0.5441637826236452 1105 76-77 0.5903229086003599 1105 78-79 0.5191911886218001 1105 80-81 0.6838485255142182 1105 82-83 0.5688109263758712 1105 84-85 0.3176906872826599 1105 86-87 0.5886247000032938 1105 88-89 0.7413494422371052 1105 90-91 0.5605170271137965 1105 92-93 0.533607885689289 1105 94-95 0.7281289762156895 1105 96-97 0.7663548351449414 1105 98-99 0.3766171167633665 1105 100-101 0.4875103381640429 1106 1 -0.025016194782974566 1106 2 -0.1058075005587682 1106 3 -0.1980571247648122 1106 4 -0.0072936852150746745 1106 5 0.2573813586655618 1106 6 0.024489905591316585 1106 7 0.3299420144967655 1106 8 0.28024137913894975 1106 9 0.3247737192012323 1106 10-11 0.1736078739033431 1106 12-13 0.059896817135516756 1106 14-15 0.10847434416613311 1106 16-17 0.010482502797785287 1106 18-19 0.04880947826898563 1106 20-21 0.06135385447021946 1106 22-23 -0.01010859606986969 1106 24-25 -0.07360858756725719 1106 26-27 -0.10974433321354127 1106 28-29 -0.1222616042167104 1106 30-31 0.03076520560666296 1106 32-33 -0.09513605429457783 1106 34-35 -0.06783611788266342 1106 36-37 0.012369853759096827 1106 38-39 -0.2303234983046849 1106 40-41 -0.21643084051635242 1106 42-43 -0.009158743829736693 1106 44-45 -0.07644550335123057 1106 46-47 0.10905011032396317 1106 48-49 -0.12818426506466807 1106 50-51 -0.08337231628333797 1106 52-53 -0.17885626971384738 1106 54-55 -0.025997237018344777 1106 56-57 -0.03479806631392535 1106 58-59 -0.0980501867964918 1106 60-61 0.052123433692194965 1106 62-63 0.1179027839195399 1106 64-65 -0.16598882744948895 1106 66-67 0.11242850924770664 1106 68-69 -0.006173525750476472 1106 70-71 0.11844381640058899 1106 72-73 0.18847999739059418 1106 74-75 0.08029541793671413 1106 76-77 -0.016302027059726498 1106 78-79 0.01608156447759157 1106 80-81 0.091967254102169 1106 82-83 0.24229402642513165 1106 84-85 0.22827342702839815 1106 86-87 0.21729760971709666 1106 88-89 0.2391665895851922 1106 90-91 0.23073387337876028 1106 92-93 0.253426239583721 1106 94-95 0.1921667001600973 1106 96-97 0.28277770196546825 1106 98-99 0.2794287018221162 1106 100-101 0.7179474068098877 1107 1 0.3039062136071138 1107 2 0.3282626118383405 1107 3 0.3901641596541907 1107 4 0.4501876167529204 1107 5 0.3119002238308184 1107 6 0.19789900599661792 1107 7 0.3691013342644496 1107 8 0.4404177047145197 1107 9 0.46886346975486504 1107 10-11 0.5547865811276793 1107 12-13 0.6226135680651979 1107 14-15 0.6931628495770781 1107 16-17 0.4271018150444732 1107 18-19 0.90519187913808 1107 20-21 0.909013989012486 1107 22-23 0.7700673116725767 1107 24-25 0.8401611028184064 1107 26-27 0.9551367932645434 1107 28-29 0.6055451897497761 1107 30-31 0.5706369308396049 1107 32-33 0.7027517992966992 1107 34-35 0.5990046459162812 1107 36-37 0.898364087662074 1107 38-39 0.728931505246905 1107 40-41 0.758045823504041 1107 42-43 0.8224607773335038 1107 44-45 0.7030973872182713 1107 46-47 0.8243010191109903 1107 48-49 0.7697746201565678 1107 50-51 0.6404128339276625 1107 52-53 0.4582264194600967 1107 54-55 0.5773078229407105 1107 56-57 0.7805969948986302 1107 58-59 0.610287673062281 1107 60-61 0.5711661780201069 1107 62-63 0.7053970387141746 1107 64-65 0.32421716427741387 1107 66-67 0.33162376266349725 1107 68-69 0.2977645513815119 1107 70-71 0.44369765001944117 1107 72-73 0.34444456514223276 1107 74-75 0.1366778188058042 1107 76-77 0.39760972658586624 1107 78-79 0.2999908111244771 1107 80-81 0.31800243565065855 1107 82-83 0.2170777977868461 1107 84-85 0.3157008285242391 1107 86-87 0.430160602237887 1107 88-89 0.4869186474132974 1107 90-91 0.5865835370231025 1107 92-93 0.5887832779825857 1107 94-95 0.5600019067785773 1107 96-97 0.6186006242902913 1107 98-99 0.6423000510052503 1107 100-101 1.1429672538829685 1108 1 0.05736082985700719 1108 2 0.09060578048072188 1108 3 0.07373039656688718 1108 4 0.23387499236942944 1108 5 0.2594471650785479 1108 6 0.07910952775831959 1108 7 0.21462136541440202 1108 8 0.06840939806053825 1108 9 0.3542900510475846 1108 10-11 0.22722752600957108 1108 12-13 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-1.6660231441563909 2316 64-65 -1.7518576197172209 2316 66-67 -2.4631784770814917 2316 68-69 -1.9296754704287373 2316 70-71 -1.343958586873434 2316 72-73 -1.4221605854323833 2316 74-75 -1.4950833589477384 2316 76-77 -2.2330378700922644 2316 78-79 -2.268444093665387 2316 80-81 -2.6082264880973476 2316 82-83 -2.2476357329572174 2316 84-85 -2.393660802211439 2316 86-87 -2.311390022709684 2316 88-89 -2.595446506516744 2316 90-91 -2.5485970266879967 2316 92-93 -2.9187884441883725 2316 94-95 -2.991905441598238 2316 96-97 -3.276069495666089 2316 98-99 -4.109202549447733 2316 100-101 -4.498094737560702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2020.0 3 774.0 4 710.0 5 866.0 6 988.0 7 1263.0 8 1519.0 9 1801.0 10 2551.0 11 3337.0 12 4604.0 13 5888.0 14 7111.0 15 9048.0 16 11749.0 17 15477.0 18 12490.0 19 7569.0 20 7167.0 21 7372.0 22 8147.0 23 9212.0 24 10455.0 25 12045.0 26 13863.0 27 16418.0 28 19609.0 29 23530.0 30 29037.0 31 35790.0 32 44953.0 33 58493.0 34 77974.0 35 107510.0 36 149894.0 37 192822.0 38 155336.0 39 25401.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.598019100099215 19.21319430614605 14.669840881228225 21.518945712526516 2 15.302432264800533 20.59016698926961 40.57626415745825 23.53113658847161 3 18.51276359600444 26.826616973367496 30.229785834669297 24.430833595958763 4 11.99070567262806 16.979873559868096 38.51060505413315 32.51881571337069 5 12.597607792357326 38.68115138129019 34.1784679380391 14.542772888313383 6 29.972243522193757 37.251989884468344 18.010362296521805 14.765404296816092 7 27.71244063466771 34.18200556604239 21.278143141945044 16.827410657344856 8 27.01461896674119 32.95786254827384 20.425166074787786 19.602352410197188 9 27.641406703914694 16.12167974245508 21.01139731273364 35.22551624089658 10-11 24.423871753610197 26.90739821863769 27.327106698064846 21.34162332968727 12-13 26.20962307176024 24.854035625404634 27.78597892191734 21.15036238091779 14-15 23.346968420042895 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30.923285191136184 21.623750962554684 40-41 21.158559142829738 26.18968454417158 31.35709490164556 21.294661411353122 42-43 21.19285085984605 26.269856565968418 30.528850764850578 22.00844180933495 44-45 21.21206446927936 26.27474800988322 30.617929547810757 21.895257973026666 46-47 21.970332567723162 26.675204232489126 29.72854103078319 21.625922169004525 48-49 21.647435472932088 27.09563684442486 29.083639137096757 22.173288545546303 50-51 21.842414986383115 27.152063815531974 29.170185256313935 21.835335941770975 52-53 22.101605648198767 27.093880432940885 28.871068144025596 21.93344577483475 54-55 21.521255902514373 27.22283701592988 29.133472754235722 22.122434327320025 56-57 21.877560452950693 27.450247858696336 28.373038527480936 22.29915316087203 58-59 21.942437171677312 26.805335189432654 28.743734979708048 22.508492659181982 60-61 21.57198471302206 27.215451758696663 29.196383226706907 22.01618030157437 62-63 21.563969895445176 27.258560931397913 29.42917076374392 21.748298409412996 64-65 21.529392474981993 27.184185779277037 29.139406162894844 22.147015582846127 66-67 21.394138438302246 27.373395391662136 28.745867307162694 22.486598862872924 68-69 21.79336809961096 27.115641262437894 29.055921091694003 22.035069546257144 70-71 21.884383252809208 27.1037897950403 28.776617950256828 22.235209001893665 72-73 21.701627794496254 27.267350436875176 28.636595940584424 22.39442582804414 74-75 21.521520104277897 28.00518283799201 28.31447239458259 22.1588246631475 76-77 21.705234110710876 27.350755249616242 28.73259737999882 22.21141325967406 78-79 21.544231290223397 27.40488352660666 28.63611298803172 22.41477219513822 80-81 21.786546314991277 27.671094585088206 28.162246361584913 22.380112738335605 82-83 22.031211392811485 27.4886607618744 28.342180778352894 22.13794706696122 84-85 21.55262816542708 27.549149644017913 28.21516591487488 22.68305627568013 86-87 21.773449143187037 27.74914807410625 27.98089566745271 22.496507115254005 88-89 21.73876108134771 27.387154086111376 27.985147226066342 22.888937606474567 90-91 21.386119710712357 27.273636621908143 28.48041772594774 22.859825941431765 92-93 21.824394660045392 27.292563354522688 28.50801646365722 22.3750255217747 94-95 21.35748817644332 26.906418632692166 28.789053133499433 22.947040057365086 96-97 21.5058910145187 26.784716839893292 28.759760696517638 22.949631449070367 98-99 21.261577648127204 27.142127564715146 28.288243924989448 23.3080508621682 100-101 21.348642639991887 26.90148734382524 28.608181716005692 23.14168830017718 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 298.0 1 234.5 2 241.0 3 301.0 4 278.0 5 293.5 6 487.5 7 926.5 8 1477.0 9 1740.0 10 1608.5 11 1405.5 12 1385.0 13 1519.0 14 1640.5 15 1718.0 16 1799.5 17 1921.0 18 2060.0 19 2266.5 20 2549.5 21 2873.5 22 3261.5 23 3753.0 24 4302.5 25 5244.5 26 6877.0 27 8456.5 28 9610.5 29 11253.0 30 13774.5 31 17110.5 32 22242.0 33 25910.5 34 27823.0 35 30567.5 36 35470.5 37 39258.0 38 38924.0 39 38080.5 40 35983.5 41 34062.0 42 32491.5 43 32535.0 44 33966.0 45 35261.0 46 35161.0 47 35078.5 48 38906.5 49 37807.0 50 32538.0 51 30148.0 52 29808.0 53 30773.5 54 31282.0 55 29613.0 56 26925.0 57 24544.0 58 23806.5 59 22116.5 60 18527.0 61 16152.0 62 13936.0 63 10331.0 64 7012.0 65 5408.5 66 4233.0 67 3395.0 68 2721.0 69 2098.5 70 1653.0 71 1305.0 72 1017.5 73 901.5 74 734.0 75 549.0 76 407.5 77 259.5 78 176.0 79 114.5 80 74.0 81 59.0 82 40.0 83 26.0 84 16.5 85 9.5 86 8.0 87 7.0 88 5.5 89 4.0 90 2.5 91 3.0 92 2.0 93 0.5 94 0.5 95 1.0 96 1.5 97 2.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01297045294283136 2 0.00502376698489947 3 0.007398638650488311 4 0.033339544536151025 5 0.0450312204282807 6 0.05818435580692659 7 0.027585047807993455 8 0.02365737543798114 9 0.013883865121903989 10-11 0.04247366632687733 12-13 0.039870441616520334 14-15 0.017674525665055407 16-17 0.02155652742611409 18-19 0.02790474207066887 20-21 0.021739209861928614 22-23 0.01392953573085762 24-25 0.0034709662804759973 26-27 0.0026488953193106297 28-29 0.0019181655760525248 30-31 0.006576567689322942 32-33 9.134121790726309E-4 34-35 0.0015984713133771042 36-37 0.004795413940131312 38-39 0.004841084549084944 40-41 0.0028315777551251556 42-43 6.393885253508416E-4 44-45 0.001004753396979894 46-47 0.002420542274542472 48-49 3.196942626754208E-4 50-51 0.0014157888775625778 52-53 4.1103548058268386E-4 54-55 0.0026945659282642614 56-57 0.0017354831402379986 58-59 0.0024662128834961033 60-61 7.307297432581048E-4 62-63 0.0010504240059335255 64-65 0.0031512720178005767 66-67 0.0017811537491916303 68-69 0.005206449420713996 70-71 0.008494733265375467 72-73 0.0029685895819860503 74-75 0.0026488953193106297 76-77 0.0023292010566352086 78-79 0.03301985027347561 80-81 0.020643115247041457 82-83 0.00324261323570784 84-85 0.007124614996766521 86-87 0.0064395558624620475 88-89 0.004658402113270417 90-91 0.009773510316077151 92-93 0.01301612355178499 94-95 0.004886755158038576 96-97 0.00324261323570784 98-99 0.02100848011867051 100-101 0.03918538248221587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1094796.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.95060230871312 #Duplication Level Percentage of deduplicated Percentage of total 1 75.45439372939416 33.917204449750535 2 12.692243612801438 11.410479900886834 3 4.572753997459429 6.1664413918613095 4 2.1117281866342537 3.7969381560598507 5 1.1754232771271185 2.64179921372727 6 0.7596672647818015 2.0488500663692775 7 0.5187021873621822 1.632118301854394 8 0.383142886167363 1.377800280281734 9 0.28203669348727695 1.1409947320869906 >10 1.7205053925281293 15.179724811189462 >50 0.1981395768016974 6.248494477096853 >100 0.12247142397062188 10.166172630660517 >500 0.006747173464935585 2.062092957948115 >1k 0.0016356784157419597 1.0831111783847454 >5k 4.0891960393548994E-4 1.127777451842105 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7011 0.6403932787478215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5260 0.4804548061922038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3235 0.2954888399299961 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1658 0.15144373929024219 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1320 0.12057040763758728 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1259 0.11499859334524423 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1169 0.10677788373359057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.13412179072631E-5 0.0 2 0.0 0.0 0.0 9.13412179072631E-5 0.0 3 0.0 0.0 0.0 9.13412179072631E-5 0.0 4 0.0 0.0 0.0 9.13412179072631E-5 0.0 5 0.0 0.0 0.0 9.13412179072631E-5 0.0 6 0.0 0.0 0.0 9.13412179072631E-5 0.0 7 0.0 0.0 0.0 9.13412179072631E-5 0.0 8 0.0 0.0 0.0 9.13412179072631E-5 0.0 9 0.0 0.0 0.0 9.13412179072631E-5 0.0 10-11 0.0 0.0 0.0 9.13412179072631E-5 0.0 12-13 0.0 0.0 0.0 9.13412179072631E-5 0.0 14-15 0.0 0.0 0.0 9.13412179072631E-5 0.0 16-17 0.0 0.0 0.0 9.13412179072631E-5 0.0 18-19 0.0 0.0 0.0 9.13412179072631E-5 0.0 20-21 0.0 0.0 0.0 1.3701182686089465E-4 0.0 22-23 0.0 0.0 0.0 3.1969426267542084E-4 0.0 24-25 0.0 0.0 0.0 5.02376698489947E-4 0.0 26-27 0.0 0.0 0.0 8.677415701189993E-4 0.0 28-29 0.0 0.0 0.0 0.0016898125312843671 0.0 30-31 0.0 0.0 0.0 0.003014260190939682 0.0 32-33 0.0 0.0 0.0 0.005571814292343049 0.0 34-35 0.0 0.0 0.0 0.007307297432581046 0.0 36-37 0.0 0.0 0.0 0.009636498489216255 0.0 38-39 0.0 0.0 0.0 0.013244476596553149 0.0 40-41 0.0 0.0 0.0 0.019090314542617986 0.0 42-43 0.0 0.0 0.0 0.03073631982579403 0.0 44-45 0.0 0.0 0.0 0.047040727222240494 0.0 46-47 0.0 0.0 0.0 0.06311678157391878 0.0 48-49 0.0 0.0 0.0 0.09385310139971281 0.0 50-51 0.0 0.0 0.0 0.12737532837167836 0.0 52-53 0.0 0.0 0.0 0.16254169726597467 0.0 54-55 0.0 0.0 0.0 0.22424268996233088 0.0 56-57 0.0 0.0 0.0 0.2839798464736809 0.0 58-59 0.0 0.0 0.0 0.3639490827514898 0.0 60-61 0.0 0.0 0.0 0.45533597126770653 0.0 62-63 0.0 0.0 0.0 0.5775505208276245 0.0 64-65 0.0 0.0 0.0 0.674783247289906 4.567060895363155E-5 66-67 0.0 0.0 0.0 0.8144896400790649 9.13412179072631E-5 68-69 0.0 0.0 0.0 0.9869418594879777 9.13412179072631E-5 70-71 0.0 0.0 0.0 1.099839604821355 9.13412179072631E-5 72-73 0.0 0.0 0.0 1.2666286687200172 9.13412179072631E-5 74-75 0.0 0.0 0.0 1.477124505387305 9.13412179072631E-5 76-77 0.0 0.0 0.0 1.626604408492541 9.13412179072631E-5 78-79 0.0 0.0 0.0 1.7701928030427587 9.13412179072631E-5 80-81 0.0 0.0 0.0 1.994618175440904 9.13412179072631E-5 82-83 0.0 0.0 0.0 2.2742136434550364 9.13412179072631E-5 84-85 0.0 0.0 0.0 2.604457816798746 9.13412179072631E-5 86-87 0.0 0.0 0.0 3.0747737478032438 9.13412179072631E-5 88-89 0.0 0.0 0.0 3.589253157665903 9.13412179072631E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6325 0.0 32.37995 1 GTATCAA 9385 0.0 30.581736 1 GTACATG 7550 0.0 27.378002 1 TACATGG 7665 0.0 26.532076 2 ACATGGG 7610 0.0 26.037003 3 CCTATAC 315 0.0 25.64354 3 CATGGGA 4760 0.0 25.55481 4 GTATAGG 525 0.0 25.34301 1 ATCAACG 11525 0.0 24.778395 3 TCAACGC 11590 0.0 24.598433 4 CAACGCA 11730 0.0 24.34869 5 TATCAAC 11785 0.0 24.272053 2 AACGCAG 11905 0.0 23.990772 6 GAGTACT 6325 0.0 23.811607 12-13 AGGTAAG 365 0.0 23.432535 2 GTACTTT 6655 0.0 22.5569 14-15 GGTAAGG 410 0.0 22.019596 3 ACGCAGA 13185 0.0 21.660759 7 GGGTATG 220 5.6132194E-8 21.600143 7 CGCAGAG 13205 0.0 21.555977 8 >>END_MODULE