FastQCFastQC Report
Thu 26 May 2016
SRR936654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094796
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68290.6237691770886997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56310.5143423980357984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.32627083036474375No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23630.21583929791486267No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20600.18816290888896195No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15800.14431912429347568No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA14710.134362931541584No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA14370.13125733013273708No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.1304352591715717No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA11880.10851336687382855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11390.10403764719637265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG5800.031.9399642
TAAGGTA3250.029.225714
GGTATCA80850.027.1026171
GGTAAGG7200.027.0451643
GTATCAA111550.025.80381
GTACATG81900.023.9388181
GTAAGGT8550.023.8847544
TAAGGTG8250.023.6008955
TACATGG83050.022.6492252
ACATGGG81200.022.343213
GAGTACT69900.022.182212-13
GGTGTGC10850.021.8846118
GTATAGG5500.021.8425711
CATATAG2858.367351E-1121.6639063
ATACCGT1100.00288093521.5861856
GTACTTT71850.021.48103314-15
ATAGGGG4000.021.3722763
ATCAACG135900.021.2132643
TATAGGG4050.021.1113152
TCAACGC136800.021.072744
Produced by FastQC (version 0.11.5)